LOCUS       sequence-441801-        9487 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lad-2 fluorescent neural reporter driving nuclear mNeptune2.5 
            expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9487)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 9487)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY100
FEATURES             Location/Qualifiers
     source          1..9487
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1161..1172
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             5162..5182
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5207..5227
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5249..5269
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(7078..7100)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7200..7219
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7297..7315)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7383..7487
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7488..7556,7557..8348)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7706..7725)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8519..9107
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9008..9027
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    9395..9416
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9431..9436,9437..9454,9455..9461)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9469..9485
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(9474..9487,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcactagtt ggagaaccgt
      181 cctaagtggc ctgagaggta ggcgtaggtt gcattttatt aggtaggtat tcattgcctg
      241 gtaggcaagc gtaggcatcc ttgaaggcag gcataaggta ctttgtaggc aaacataaaa
      301 ttcattctcg ccgctttgaa gttggcgtag gcattatttg caggctgtat aggtaggctt
      361 aggctccgtt tcagggcgtt ggtttccttg aatgcaagta ttggttgaaa tagaggcgaa
      421 cataggttgc ctaggtcgtg ttgggtgcgt tgtaggcaca tattggttgc ctagtaggca
      481 ggtaggtgcc agttgtttga taggcgtagg ctgcccaata ggcaggcagg catttccgtg
      541 cctcagcttg caccccattt ttccaaattt ccttcaattt ccatacaatt tcacaatacc
      601 caaaccacta atcagaaaaa attccctcat cacccaaaag gtgtgaacca tttctcctaa
      661 aactccgtcc acacactcct tacatgtcca tctgttgtta tgagttcaca caacgggtga
      721 gctaatcgaa tcgaagagag agagtccctt tggaatgccc cctcctcccc attttcctta
      781 tccaccacca gtaccactac ccgggcggtc tctaactcaa ttgggcaccc ccccccctta
      841 ggccgcccat tttgcgtgga attatcaatg tgtgtatgtg tcgtctccat ttggttttca
      901 tggtttcatc gagccgcccc tgatgtttct ctctgtgtga atttttgata atgttaacgg
      961 gaaggggcat aatgtttttg tggataataa atgagtccag ggtgttgaga aattagagta
     1021 gaatttttag ccattttttg ctgtgttcca ctaacgtttt caaaattttg aaaaaatcct
     1081 tttggcgcga aattcaaatt tcatcgctac atccatttaa gggaagttcg aagttcaatt
     1141 gtttctctaa aaattttggg tgggagcttc aaatttcgat atttttgagg ggaaaatatt
     1201 cactcgattt taattttctt gaatttttga aggaaaattt gatttttaga atttttattt
     1261 tgttccaaaa gtcttgacga gctcaaaatt aaagttaatt tttttttcat aattgagccg
     1321 caaaattaaa acaaaattaa ataagattaa aggcgcgtaa ttggaattac attttttaaa
     1381 aggtggaaat taaaattcaa aatttattta ttttaactaa aaattaaccg cagaagttct
     1441 tatgctaaat tttccagaaa aaaaaatcca gaattcaagc ttgaacagtt cttaaaaagt
     1501 atcattttgg agctttactc aaaatttaaa catttgaaga aaaaacaata ttttgtttta
     1561 attccgacgg aagtttaaaa aaattacgat ccctacctac ctactcccac ctactcctac
     1621 ctgcctgcct accttcctac ctgcgtacct acctgccttc ctacctacct acctacatac
     1681 ctacctacct tcctaccttc ctatctacct tccttcctac ctactagatt tttttttgcc
     1741 tatttaggta tataccccaa tgccaccctc tggacccatt ctattagctc ccttacccgg
     1801 ttttatgact catttccctc ttttttttct gctgacccaa tccgaaggct acccacattt
     1861 tgtaattgct ccgtctgatt tttgccgccg tctttctttt cactcctcca atattttccg
     1921 atttaaaccg gtttttatgt cctttccggg gcggctcatc ccctggttct tcccacactc
     1981 attttctatt catcagtgtc ggtgagaggg tacaagtaat tacccactac taggtggcct
     2041 agctactttt tatggggtcc cccaccccac gttgcaacac atacacaaat gagttgagta
     2101 gaaagaagac atgaaaagga agtcgagaat ggacccgcaa acagtgaatt gtacttccga
     2161 ttgatgcgaa tttcacagag aagacttgat gatgacgctg tgctttgcgg cggcggctat
     2221 agataaaact gcaacatttt ggagccgctt ccttttgttt tgaacacagg cttagagccg
     2281 gacgggctct ttggaactgc tcaactgttc aaaatttaaa acttttcatc agttgcgtta
     2341 tttatttgcg tccgaccaaa tatcgcaaac aagttgccgg ttgccgaaaa gttgccgaaa
     2401 ctttttgggt tcggcaattt cggcaattgc cggttgccga aatttttaga gttcggcaat
     2461 ttcggcaatt gccgatatca atgtcaaaca ttttgaagct cagaaaaaat catacagaaa
     2521 gcctgaaaaa tgctaaaatc taaaacgctc aaaaatatga tttcaaggaa aattttaaaa
     2581 cctagttgcc ggttgccgaa acattttggg ctcgacaact tcggcaatta ccggttgccg
     2641 aaaatctcgg ttaccgcaca tcccggtacc tacttttcta ccgtattaaa attatcaatt
     2701 attggactaa attttgaaaa attctagaag tttttatttc aattttgagc caaaaaactg
     2761 ggaaactctg ttttacgagt ttttggaagc ccgggttcaa aaaatgtttc ttccggtttt
     2821 atgatcaaat tatgtttgaa atatttatat ttttgaatat tgtattttgt tcacagctgc
     2881 acctcgtaga aacgttcttt aaaatttttt ggcggaattt ttcagaaaat ttttgagttt
     2941 ttggggtgtg aaaattttct ggaagcttcg tgagaaacct ataaaaattg acacattttt
     3001 tctcaattct cgataaatta aaaaaattct cgaaacaaaa aaatgtcctt catctacttt
     3061 ttcacatttt caagagcatt aaaattttgc aactctggaa aaatccactg tttcagtaat
     3121 tttacaagct tcttgccata tatgtggtgt tagagcgtcc gatttcggtt tgatctacgt
     3181 agatctacaa aaattgcggg agaagagaca cagggttatc aactgatttc gctgacgtca
     3241 caatttttgc gcaaaaattc ccgcattttt tgtagatcaa accgtaatgg gacagtctgg
     3301 cccaccatgc gtatattttt atccgaaatt ttatctctcc aaaaataaaa gcaatctttt
     3361 tttctaaaat ttccaaaatc ccaaaaactc gccacttgaa aatagtttca ttgtccatca
     3421 tcaacatcag ttccctctct cctcaaaact agaatagcag atgatagtct gcctcatctt
     3481 tttccatgcc atctccagtc atcgtcttcc tgtagggccc ccctcctgtg tctctgcgtc
     3541 tcttcgtctc ccctactctc tcacttcctg attggctcga ttgtccatga cgccgaggat
     3601 aagacaagat tttttcttgt gcaatttctg tgacttctgg gaccaaaaaa atatcggagc
     3661 cactgcaggc gatgtttttc tgtttacttt gtactttttg ttttgatttt tctgaaaaaa
     3721 ttaccgaaaa tactttattt tttattattc cgaaacgacg tttttccgat cgagagcagt
     3781 tttttttgta gatttttgat tattttgaaa aggtttttgg gatcggaaat tttttagtca
     3841 agcttaaaat tttttttaag tgttggtgta gatttacggg gtctaaaagt gtgaaaaaaa
     3901 attcagaaaa taaaagttcc aaaaaaattc aaaactatca cccagacaca aaatttagcg
     3961 attttccctt caaaactaca gtacctcggg tctcgaagcg acaatttttg atattttttt
     4021 tattacatgc aagaaggtgt gcgcctttac aaagtactgt agtttcaatt tttcttttcg
     4081 agttttcatg catagttttt cacatgattt tttattttct ataatattta agtttttaaa
     4141 tgaataaaca tatttttgct tgagaaacta taaaaattgg caaatttcga aactacatcg
     4201 tttcgagacc agggactgta tttttagagc gaaaattctg gaaattttca actggaaatt
     4261 ttctcttttt cttaatttag gaggacccta aaaaaagagt attcttaaaa tttcaaaaaa
     4321 taaaaaatat atccctcatt tcggcttgat ctacgttgat ctacaaaaaa tgcgggagaa
     4381 gagacgcaga gttctcaaga acttgctgac gtcacatttt cagggcaaaa aactcccgca
     4441 ttttttgtag atcaaaccgt aatgggacag cctggcacca cgtgccctag attaatcaaa
     4501 gattttaaga aaaatccgct ccaggagcat ttctaaagac tacttaaata cgtggtgtca
     4561 ggctgtctca ttacagctta atctacaaaa aatgcgggaa tatttttcca gaaaagttgt
     4621 gacgtcagca cagcagtgca aaatcagatg agatgtctgc gtctcagctc ccgcattttt
     4681 cgaagatcaa aacgaaatgg gactttctga ctccacgtgc ttacagagtg ttcaccattt
     4741 tctccaatat tcgatcaccc tcatgcacca tgccattccc ccctcttcac cccccgtttc
     4801 ccaaatacca gacgggctcc aatttctgtc aatgatttgc atctcattga cattcgcccc
     4861 gcggacactc cataatcata cccccgcccc gtgactccgc ctcctttttc gttatttgat
     4921 taatttcgaa cattttagac tgcctgccta taatgcatca tatcctgaga cgtcggtaga
     4981 tttttggtag cagacttttt ttttggattt ttccaacagg atccccggga ttggccaaag
     5041 gacccaaagg tatgtttcga atgatactaa cataacatag aacattttca ggaggaccct
     5101 tggagggtac cggttttccc agtcacgacg cgtggtaccg agctcagaaa aaatgaccgc
     5161 tccaaagaag aaacgcaaag taccgagctc agaaaaaatg acggcaccta aaaagaaacg
     5221 caaagtaccg gtcgccgaga agacagcccc aaaaaagaag cgtaaagttc cacgcgtcat
     5281 gggagtaagt ttaaacagtt cggtactaac taaccataca tatttaaatt ttcagcgcgc
     5341 tgtctccaag ggagaggagc tcatcaagga gaacatgcac accaagctct acatggaggg
     5401 aaccgtcaac aaccaccact tcaagtgcac ccacgaaggg gagggcaagc cctacgaggg
     5461 cacccagacc aacaggatta aggtggtgga gggaggcccc ctgccgttcg cattcgacat
     5521 cctggccacc tgctttatgt acgggagcaa gaccttcatc aaccacaccc aaggaatccc
     5581 agacttcttc aagcaatcct tcgtaagttt aaacatgatt ttactaacta actaatctga
     5641 tttaaatttt cagcctgagg gcttcacatg ggagagagtc accacatacg aagacggggg
     5701 cgtgcttacc gttacccagg acaccagcct ccaggacggc tgcttgatct acaacgtcaa
     5761 gctcagaggg gtgaacttcc catccaacgg ccctgtgatg cagaagaaaa cactcggctg
     5821 ggaggccagt accgagaccc tgtaccccgc tgacggcggc ctggaaggca gatgcgacat
     5881 ggccctgaag ctcgtgggcg ggggccacct gcactgcaac ctgaagacca catacagatc
     5941 caagaaaccc gctaagaacc tcaagatgcc aggagtaagt ttaaacatat atatactaac
     6001 taaccctgat tatttaaatt ttcaggtcta ctttgtggac cgcagactgg aaagaatcaa
     6061 ggaggccgac aatgagacct acgtcgagca gcacgaggtg gctgtggcca gatactgcga
     6121 cctccctagc aaactggggc acaaacttaa tggcatggac gagctgtaca agtaagcgcc
     6181 ggtcgctacc attaccaact tgtctggtgt caaaaataat aggggccgct gtcatcagag
     6241 taagtttaaa ctgagttcta ctaactaacg agtaatattt aaattttcag catctcgcgc
     6301 ccgtgcctct gacttctaag tccaattact cttcaacatc cctacatgct ctttctccct
     6361 gtgctcccac cccctatttt tgttattatc aaaaaacttc tcttaatttc tttgtttttt
     6421 agcttctttt aagtcacctc taacaatgaa attgtgtaga ttcaaaaata gaattaattc
     6481 gtaataaaaa gtcgaaaaaa attgtgctcc ctccccccat taataataat tctatcccaa
     6541 aatctacaca atgttctgtg tacacttctt atgtttttta cttctgataa atttttttga
     6601 aacatcatag aaaaaaccgc acacaaaata ccttatcata tgttacgttt cagtttatga
     6661 ccgcaatttt tatttcttcg cacgtctggg cctctcatga cgtcaaatca tgctcatcgt
     6721 gaaaaagttt tggagtattt ttggaatttt tcaatcaagt gaaagtttat gaaattaatt
     6781 ttcctgcttt tgctttttgg ggtttcccct attgtttgtc aagatttcga ggacggcgtt
     6841 tttcttgcta aaatcacaag tattgatgag cacgatgcaa gaaagatcgg aagaaggttt
     6901 gggtttgagg ctcagtggaa ggtgagtaga agttgataat ttgaaagtgg agtagtgtct
     6961 atggggtttt tgccttaaat gacagaatac attcccaata taccaaacat aactgtttcc
     7021 tactagtcgg ccgtacgggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct
     7081 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
     7141 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc
     7201 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
     7261 cgtaaggaga aaataccgca tcaggcggcc ttaagggcct cgtgatacgc ctatttttat
     7321 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     7381 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     7441 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     7501 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     7561 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     7621 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     7681 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     7741 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     7801 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     7861 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     7921 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     7981 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     8041 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     8101 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     8161 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     8221 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     8281 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     8341 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     8401 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     8461 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     8521 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     8581 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     8641 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     8701 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     8761 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     8821 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     8881 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     8941 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     9001 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     9061 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     9121 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     9181 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     9241 gcagccgaac gaccgagcgc agcgagtcag tgagcgatga agcggaagag cgcccaatac
     9301 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     9361 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     9421 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     9481 aacaatt
//
