LOCUS       sequence-441802-       16852 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  encodes three Fluorescent Protein FLP-OUT cassettes responsive to 
            three different recombinases (mFLP5, B3 and KD).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16852)
  TITLE     Multigenic Drosophila vectors and transgenic lines for 
            recombinase-directed fluorescent labeling and lineage tracing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: tRECs/3xUAS-FP attB
FEATURES             Location/Qualifiers
     source          1..16852
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      4..432
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(91..110)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     301..322
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     623..642
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(675..693)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(1145..1216,1618..1891,1966..2620,2682..2997,3201..3332,
                     3403..4017)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    4800..4894
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        4913..5151
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     intron          5683..5748
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5878..5898
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    6170..6304
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             6387..7100
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(6417..6445)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     7040..7060
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             7101..7133
                     /codon_start=1
                     /product="HSV (herpes simplex virus) epitope tag"
                     /label=HSV tag
                     /translation="QPELAPEDPED"
     intron          7238..7303
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             7433..7453
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7725..7859
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    7875..7969
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        7988..8226
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     intron          8741..8806
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8936..8956
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9228..9362
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             9413..10111
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     CDS             10112..10138
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             10142..10168
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             10172..10198
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     intron          10298..10363
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10493..10513
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    10785..10919
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    10935..11029
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        11048..11286
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     intron          11436..11501
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             11631..11651
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11923..12057
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             12926..13636
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(13071..13089)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     13339..13358
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     intron          13726..13791
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             13921..13941
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    14213..14347
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    14376..14445
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(14664..14682)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(14703..14719)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14703..14719)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(14716..14738)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    14727..14743
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(14751..14757,14758..14775,14776..14781))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14796..14817
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(14934..14951)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(15105..15693)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(15185..15204)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(15864..16655,16656..16724))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     16487..16506
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(16725..16829)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
       61 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
      121 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
      181 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
      241 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
      301 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
      361 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
      421 ttaacgcgaa ttttaacaaa atattaacgc ttacaaktyc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtacgtaccg
      661 ggcccctagt atgtatgtaa gttaataaaa cccatttttg cggaaagtag ataaaaaaaa
      721 catttttttt ttttactgca ctggatatca ttgaacttat ctgatcagtt ttaaatttac
      781 ttcgatccaa gggtatttga tgtaccaggt tctttcgatt acctctcact caaaatgaca
      841 ttccactcaa agtcagcgct gtttgcctcc ttctctgtcc acagaaatat cgccgtctct
      901 ttcgccgctg cgtccgctat ctctttcgcc accgtttgta gcgttacgta gcgtcaatgt
      961 ccgccttcag ttgcattttg tcagcggttt cgtgacgaag ctccaagcgg tttacgccat
     1021 caattaaaca caaagtgctg tgccaaaact cctctcgctt cttatttttg tttgtttttt
     1081 gagtgattgg ggtggtgatt ggttttgggt gggtaagcag gggaaagtgt gaaaaatccc
     1141 ggcaatgggc caagaggatc aggagctatt aattcgcgga ggcagcaaac acccatctgc
     1201 cgagcatctg aacaatgtga gtagtacatg tgcatacatc ttaagttcac ttgatctata
     1261 ggaactgcga ttgcaacatc aaattgtctg cggcgtgaga actgcgaccc acaaaaatcc
     1321 caaaccgcaa ttgcacaaac aaatagtgac acgaaacaga ttattctggt agctgttctc
     1381 gctatataag acaatttttg agatcatatc atgatcaaga catctaaagg cattcatttt
     1441 cgactatatt cttttttaca aaaaatataa caaccagata ttttaagctg atcctagatg
     1501 cacaaaaaat aaataaaagt ataaacctac ttcgtaggat acttcggggt actttttgtt
     1561 cggggttaga tgagcataac gcttgtagtt gatatttgag atcccctatc attgcagggt
     1621 gacagcggag cggcttcgca gagctgcatt aaccagggct tcgggcaggc caaaaactac
     1681 ggcacgctcc ggccacccag tccgccggag gactccggtt cagggagcgg ccaactagcc
     1741 gagaacctca cctatgcctg gcacaatatg gacatctttg gggcggtcaa tcagccgggc
     1801 tccggatggc ggcagctggt caaccggaca cgcggactat tctgcaacga gcgacacata
     1861 ccggcgccca ggaaacattt gctcaagaac ggtgagtttc tattcgcagt cggctgatct
     1921 gtgtgaaatc ttaataaagg gtccaattac caatttgaaa ctcagtttgc ggcgtggcct
     1981 atccgggcga acttttggcc gtgatgggca gttccggtgc cggaaagacg accctgctga
     2041 atgcccttgc ctttcgatcg ccgcagggca tccaagtatc gccatccggg atgcgactgc
     2101 tcaatggcca acctgtggac gccaaggaga tgcaggccag gtgcgcctat gtccagcagg
     2161 atgacctctt tatcggctcc ctaacggcca gggaacacct gattttccaa gccatggtgc
     2221 ggatgccacg acatctgacc tatcggcagc gagtggcccg cgtggatcag gtgatccagg
     2281 agctttcgct cagcaaatgt cagcacacga tcatcggtgt gcccggcagg gtgaaaggtc
     2341 tgtccggcgg agaaaggaag cgtctggcat tcgcctccga ggctctaacc gatccgccgc
     2401 ttctgatctg cgatgagccc acctccggac tggactcctt taccgcccac agcgtcgtcc
     2461 aggtgctgaa gaagctgtcg cagaagggca agaccgtcat cctgaccatt catcagccgt
     2521 cttccgagct gtttgagctc tttgacaaga tccttctgat ggccgagggc agggtagctt
     2581 tcttgggcac tcccagcgaa gccgtcgact tcttttccta gtgagttcga tgtgtttatt
     2641 aagggtatct agtattacat aacatctcaa ctcctatcca gcgtgggtgc ccagtgtcct
     2701 accaactaca atccggcgga cttttacgta caggtgttgg ccgttgtgcc cggacgggag
     2761 atcgagtccc gtgatcggat cgccaagata tgcgacaatt ttgccattag caaagtagcc
     2821 cgggatatgg agcagttgtt ggccaccaaa aatctggaga agccactgga gcagccggag
     2881 aatgggtaca cctacaaggc cacctggttc atgcagttcc gggcggtcct gtggcgatcc
     2941 tggctgtcgg tgctcaagga accactcctc gtaaaagtgc gacttattca gacaacggtg
     3001 agtggttcca gtggaaacaa atgatataac gcttacaatt cttggaaaca aattcgctag
     3061 attttagata gaattgcctg attccacacc cttcttagtt tttttcaatg agatgtatag
     3121 tttatagttt tgcagaagat aaataaattt catttaactc gcgaatatta atgagatgcg
     3181 agtaacattt taatttgcag atggttgcca tcttgattgg cctcatcttt ttgggccaac
     3241 aactcacgca agtgggtgtg atgaatatca acggagccat cttcctcttc ctgaccaaca
     3301 tgacctttca aaacgtcttt gccacgataa atgtaagtca tgtttagaat acatttgcat
     3361 ttcaataatt tactaacttt ctaatgaatc gattcgattt aggtgttcac ctcagagctg
     3421 ccagttttta tgagggaggc ccgaagtcga ctttatcgct gtgacacata ctttctgggc
     3481 aaaacgattg ccgaattgcc gctttttctc acagtgccac tggtcttcac ggcgattgcc
     3541 tatccgatga tcggactgcg ggccggagtg ctgcacttct tcaactgcct ggcgctggtc
     3601 actctggtgg ccaatgtgtc aacgtccttc ggatatctaa tatcctgcgc cagctcctcg
     3661 acctcgatgg cgctgtctgt gggtccgccg gttatcatac cattcctgct ctttggcggc
     3721 ttcttcttga actcgggctc ggtgccagta tacctcaaat ggttgtcgta cctctcatgg
     3781 ttccgttacg ccaacgaggg tctgctgatt aaccaatggg cggacgtgga gccgggcgaa
     3841 attagctgca catcgtcgaa caccacgtgc cccagttcgg gcaaggtcat cctggagacg
     3901 cttaacttct ccgccgccga tctgccgctg gactacgtgg gtctggccat tctcatcgtg
     3961 agcttccggg tgctcgcata tctggctcta agacttcggg cccgacgcaa ggagtagccg
     4021 acatatatcc gaaataactg cttgtttttt ttttttacca ttattaccat cgtgtttact
     4081 gtttattgcc ccctcaaaaa gctaatgtaa ttatatttgt gccaataaaa acaagatatg
     4141 acctatagaa tacaagtatt tccccttcga acatccccac aagtagactt tggatttgtc
     4201 ttctaaccaa aagacttaca cacctgcata ccttacatca aaaactcgtt tatcgctaca
     4261 taaaacaccg ggatatattt tttatataca tacttttcaa atcgcgcgcc ctcttcataa
     4321 ttcacctcca ccacaccacg tttcgtagtt gctctttcgc tgtctcccac ccgctctccg
     4381 caacacattc accttttgtt cgacgacctt ggagcgactg tcgttagttc cgcgcgattc
     4441 ggttcgctca aatggttccg agtggttcat ttcgtctcaa tagaaattag taataaatat
     4501 ttgtatgtac aatttatttg ctccaatata tttgtatata tttccctcac agctatattt
     4561 attctaattt aatattatga ctttttaagg taattttttg tgacctgttc ggagtgatta
     4621 gcgttacaat ttgaactgaa agtgacatcc agtgtttgtt ccttgtgtag atgcatctca
     4681 aaaaaatggt gggcataata gtgttgttta tatatatcaa aaataacaac tataataata
     4741 agaatacatt taatttagaa aatgcttgga tttcactgga actaggctag cctgcaggtc
     4801 ggagtactgt cctccgagcg gagtactgtc ctccgagcgg agtactgtcc tccgagcgga
     4861 gtactgtcct ccgagcggag tactgtcctc cgagcggaga ctctagcgag cgccggagta
     4921 taaatagagg cgcttcgtct acggagcgac aattcaattc aaacaagcaa agtgaacacg
     4981 tcgctaagcg aaagctaagc aaataaacaa gcgcagctga acaagctaaa caatctgcag
     5041 taaagtgcaa gttaaagtga atcaattaaa agtaaccagc aaccaagtaa atcaactgca
     5101 actactgaaa tctgccaaga agtaattatt gaatacaaga agagaactct gaatagggaa
     5161 ttgggaattg gccggccaaa cgatatcaga catttgtctg ataatgcttc attatcagac
     5221 aaatgtctga tatcgtttta acgtaagcta gctagaccgg tgtcgactaa agccaaatag
     5281 aaaattattc agttcctggc ttaagttttt aaaagtgata ttatttattt ggttgtaacc
     5341 aaccaaaaga atgtaaataa ctaatacata attatgttag ttttaagtta gcaacaaatt
     5401 gattttagct atattagcta cttggttaat aaatagaata tatttattta aagataattg
     5461 cgtttttatt gtcagggagt gagtttgctt aaaaactcgt ttaggtttgt cctcccgaaa
     5521 ttatttattt aaatgcgatg gagagttggc gccgaatcga aaactttacg cgcttaaaag
     5581 cacgagttgg catccctaac gcgtaggatc tttgtgaagg aaccttactt ctgtggtgtg
     5641 acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta
     5701 agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat
     5761 ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca
     5821 gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca
     5881 aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg
     5941 agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa
     6001 aaagctgcac tgctatacaa gaaaattatg gaaaaatatt tgatgtatag tgccttgact
     6061 agagatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
     6121 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
     6181 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     6241 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     6301 gatcactagt gatctggccg gaaacgatat cagacatttg tctgataatg cttcattatc
     6361 agacaaatgt ctgatatcgt ttcaaaatga gtaaaggaga agaacttttc actggagttg
     6421 tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct gtcagtggag
     6481 agggtgaagg tgatgcaaca tacggaaaac ttacccttaa atttatttgc actactggaa
     6541 aactacctgt tccatggcca acacttgtca ctactttaac ttatggtgtt caatgctttt
     6601 caagataccc agatcatatg aaacagcatg actttttcaa gagtgccatg cccgaaggtt
     6661 atgtccagga aagaactata tttttcaaag atgacgggaa ctacaagaca cgtgctgaag
     6721 tcaagtttga aggtgatacc cttgttaata gaatcgagtt aaaaggtatt gattttaaag
     6781 aagatggaaa cattcttgga cacaaattgg aatacaacta taactcacac aatgtataca
     6841 tcatggcaga caaacaaaag aatggaatca aagttaactt caaaattaga cacaacattg
     6901 aagatggaag cgttcaacta gcagaccatt atcaacaaaa tactccaatt ggcgatggcc
     6961 ctgtcctttt accagacaac cattacctgt ccacacaatc tgccctttcg aaagatccca
     7021 acgaaaagag agaccacatg gtccttcttg agtttgtaac agctgctggg attacacatg
     7081 gcatggatga actatacaaa cagcccgagc tggcccccga ggatcccgag gattgattaa
     7141 ttaactgaag acgtcctaga ggatctttgt gaaggaacct tacttctgtg gtgtgacata
     7201 attggacaaa ctacctacag agatttaaag ctctaaggta aatataaaat ttttaagtgt
     7261 ataatgtgtt aaactactga ttctaattgt ttgtgtattt tagattccaa cctatggaac
     7321 tgatgaatgg gagcagtggt ggaatgcctt taatgaggaa aacctgtttt gctcagaaga
     7381 aatgccatct agtgatgatg aggctactgc tgactctcaa cattctactc ctccaaaaaa
     7441 gaagagaaag gtagaagacc ccaaggactt tccttcagaa ttgctaagtt ttttgagtca
     7501 tgctgtgttt agtaatagaa ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc
     7561 tgcactgcta tacaagaaaa ttatggaaaa atatttgatg tatagtgcct tgactagaga
     7621 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
     7681 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag
     7741 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     7801 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatcc
     7861 aagcttctgc aggtcggagt actgtcctcc gagcggagta ctgtcctccg agcggagtac
     7921 tgtcctccga gcggagtact gtcctccgag cggagtactg tcctccgagc ggagactcta
     7981 gcgagcgccg gagtataaat agaggcgctt cgtctacgga gcgacaattc aattcaaaca
     8041 agcaaagtga acacgtcgct aagcgaaagc taagcaaata aacaagcgca gctgaacaag
     8101 ctaaacaatc tgcagtaaag tgcaagttaa agtgaatcaa ttaaaagtaa ccagcaacca
     8161 agtaaatcaa ctgcaactac tgaaatctgc caagaagtaa ttattgaata caagaagaga
     8221 actctgaata gggaattggg aattggcgcg ccatatgcgt acgggttgct taagaataag
     8281 taattcttaa gcaacctaac gtaagctagc tagaccggtg tcgactaaag ccaaatagaa
     8341 aattattcag ttcctggctt aagtttttaa aagtgatatt atttatttgg ttgtaaccaa
     8401 ccaaaagaat gtaaataact aatacataat tatgttagtt ttaagttagc aacaaattga
     8461 ttttagctat attagctact tggttaataa atagaatata tttatttaaa gataattgcg
     8521 tttttattgt cagggagtga gtttgcttaa aaactcgttt aggtttgtcc tcccgaaatt
     8581 atttatttaa atgcgatgga gagttggcgc cgaatcgaaa actttacgcg cttaaaagca
     8641 cgagttggca tccctaacgc gtaggatctt tgtgaaggaa ccttacttct gtggtgtgac
     8701 ataattggac aaactaccta cagagattta aagctctaag gtaaatataa aatttttaag
     8761 tgtataatgt gttaaactac tgattctaat tgtttgtgta ttttagattc caacctatgg
     8821 aactgatgaa tgggagcagt ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga
     8881 agaaatgcca tctagtgatg atgaggctac tgctgactct caacattcta ctcctccaaa
     8941 aaagaagaga aaggtagaag accccaagga ctttccttca gaattgctaa gttttttgag
     9001 tcatgctgtg tttagtaata gaactcttgc ttgctttgct atttacacca caaaggaaaa
     9061 agctgcactg ctatacaaga aaattatgga aaaatatttg atgtatagtg ccttgactag
     9121 agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac
     9181 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
     9241 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     9301 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
     9361 tcactagtga tctggccggg gttgcttaag aataagtaat tcttaagcaa ccatgagcga
     9421 gctgattaag gagaacatgc acatgaagct gtacatggag ggcaccgtgg acaaccatca
     9481 cttcaagtgc acatccgagg gcgaaggcaa gccctacgag ggcacccaga ccatgagaat
     9541 caaggtggtc gagggcggcc ctctcccctt cgccttcgac atcctggcta ctagcttcct
     9601 ctacggcagc aagaccttca tcaaccacac ccagggcatc cccgacttct tcaagcagtc
     9661 cttccctgag ggcttcacat gggagagagt caccacatac gaagacgggg gcgtgctgac
     9721 cgctacccag gacaccagcc tccaggacgg ctgcctcatc tacaacgtca agatcagagg
     9781 ggtgaacttc acatccaacg gccctgtgat gcagaagaaa acactcggct gggaggcctt
     9841 caccgagacg ctgtaccccg ctgacggcgg cctggaaggc agaaacgaca tggccctgaa
     9901 gctcgtgggc gggagccatc tgatcgcaaa catcaagacc acatatagat ccaagaaacc
     9961 cgctaagaac ctcaagatgc ctggcgtcta ctatgtggac tacagactgg aaagaatcaa
    10021 ggaggccaac aacgagacct acgtcgagca gcacgaggtg gcagtggcca gatactgcga
    10081 cctccctagc aaactggggc acaagcttaa ttacccatac gatgttcctg actatgcggg
    10141 ctatccctat gacgtcccgg actatgcagg atatccatat gacgttccag attacgctgg
    10201 cggataagtt taaacctaga ggatctttgt gaaggaacct tacttctgtg gtgtgacata
    10261 attggacaaa ctacctacag agatttaaag ctctaaggta aatataaaat ttttaagtgt
    10321 ataatgtgtt aaactactga ttctaattgt ttgtgtattt tagattccaa cctatggaac
    10381 tgatgaatgg gagcagtggt ggaatgcctt taatgaggaa aacctgtttt gctcagaaga
    10441 aatgccatct agtgatgatg aggctactgc tgactctcaa cattctactc ctccaaaaaa
    10501 gaagagaaag gtagaagacc ccaaggactt tccttcagaa ttgctaagtt ttttgagtca
    10561 tgctgtgttt agtaatagaa ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc
    10621 tgcactgcta tacaagaaaa ttatggaaaa atatttgatg tatagtgcct tgactagaga
    10681 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
    10741 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag
    10801 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
    10861 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatcc
    10921 gcatgcctgc aggtcggagt actgtcctcc gagcggagta ctgtcctccg agcggagtac
    10981 tgtcctccga gcggagtact gtcctccgag cggagtactg tcctccgagc ggagactcta
    11041 gcgagcgccg gagtataaat agaggcgctt cgtctacgga gcgacaattc aattcaaaca
    11101 agcaaagtga acacgtcgct aagcgaaagc taagcaaata aacaagcgca gctgaacaag
    11161 ctaaacaatc tgcagtaaag tgcaagttaa agtgaatcaa ttaaaagtaa ccagcaacca
    11221 agtaaatcaa ctgcaactac tgaaatctgc caagaagtaa ttattgaata caagaagaga
    11281 actctgaata gggaattggg aattcgaagt ttctatactt tctagagaat agaaacttcg
    11341 cccttcggcc gccaaaatgg atctttgtga aggaacctta cttctgtggt gtgacataat
    11401 tggacaaact acctacagag atttaaagct ctaaggtaaa tataaaattt ttaagtgtat
    11461 aatgtgttaa actactgatt ctaattgttt gtgtatttta gattccaacc tatggaactg
    11521 atgaatggga gcagtggtgg aatgccttta atgaggaaaa cctgttttgc tcagaagaaa
    11581 tgccatctag tgatgatgag gctactgctg actctcaaca ttctactcct ccaaaaaaga
    11641 agagaaaggt agaagacccc aaggactttc cttcagaatt gctaagtttt ttgagtcatg
    11701 ctgtgtttag taatagaact cttgcttgct ttgctattta caccacaaag gaaaaagctg
    11761 cactgctata caagaaaatt atggaaaaat atttgatgta tagtgccttg actagagatc
    11821 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc
    11881 cctctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
    11941 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
    12001 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatctgc
    12061 gtcgcgccac ttcaacgctc gatgggagcg tcattggtgg gcggggtaac cgtcgaaatc
    12121 agtgtttacg cttccaatcg caacaaaaaa ttcactgcaa cactgaaaag catacgaaaa
    12181 cgatgaagat tgtacgagga accataaagt attttatcca caaagacacg tatagcagaa
    12241 aagccaagtt aactcggcga taagttgtgt acacaagaat aaaatcggcc agattcagtg
    12301 ttgtcagaaa taagaaaacc ccactatgtt tttctttgcc ttttctttct cccagcgatc
    12361 attcatttcg tggtgaaaga acggggtcat tgcacggagt ttcgactgcg ggaaagcaga
    12421 gctgccgttc acttcgtcta taattagcgc tttctatttt ccccgattcg ggccgctgct
    12481 gcgcttttcc gcctgctgtt tgtggcaagt gtagcagcag gctgtgcacg cagtgtggca
    12541 tgcacttggc tttccaccgt tggtatcgat tctctgggac gatgagtcat tcctttcggg
    12601 gccacagcat aatcgttgcc agctcaccga aatggtgact tcatttctta actgccgtca
    12661 agcatgcgat tgtacataca tacatattta tatatgtaca tatttatgtg actatggtag
    12721 gtcgatataa tagcaatcaa cgcaagcaaa tgtgtcagtc ctgcttacag gaacgattct
    12781 atttagtaat tttcgttgta taaagtaatt atgtatgtat gtaagcccca taaatctgaa
    12841 acaattaggc aaaaccatgc gaggatgaac tgcaagacgg aagtttctat actttctaga
    12901 gaatagaaac ttcgcggccg cccaaatggt gagcaagggc gaggaggata acatggccat
    12961 catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt
    13021 cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa
    13081 ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta
    13141 cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt
    13201 ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt
    13261 gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac
    13321 caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc
    13381 cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct
    13441 gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt
    13501 gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga
    13561 ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga
    13621 cgagctgtac aagtaaggta cctctagagg atctttgtga aggaacctta cttctgtggt
    13681 gtgacataat tggacaaact acctacagag atttaaagct ctaaggtaaa tataaaattt
    13741 ttaagtgtat aatgtgttaa actactgatt ctaattgttt gtgtatttta gattccaacc
    13801 tatggaactg atgaatggga gcagtggtgg aatgccttta atgaggaaaa cctgttttgc
    13861 tcagaagaaa tgccatctag tgatgatgag gctactgctg actctcaaca ttctactcct
    13921 ccaaaaaaga agagaaaggt agaagacccc aaggactttc cttcagaatt gctaagtttt
    13981 ttgagtcatg ctgtgtttag taatagaact cttgcttgct ttgctattta caccacaaag
    14041 gaaaaagctg cactgctata caagaaaatt atggaaaaat atttgatgta tagtgccttg
    14101 actagagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct
    14161 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt
    14221 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
    14281 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
    14341 ctggatccac tagtgtcgac gatgtaggtc acggtctcga agccgcggtg cgggtgccag
    14401 ggcgtgccct tgggctcccc gggcgcgtac tccacctcac ccatctggtc catcatgatg
    14461 aacgggtcga ggtggcggta gttgatcccg gcgaacgcgc ggcgcaccgg gaagccctcg
    14521 ccctcgaaac cgctgggcgc ggtggtcacg gtgagcacgg gacgtgcgac ggcgtcggcg
    14581 ggtgcggata cgcggggcag cgtcagcggg ttctcgacgg tcacggcggg catgtcgaca
    14641 ctagttctag ccagcttttg ttccctttag tgagggttaa tttcgagctt ggcgtaatca
    14701 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
    14761 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
    14821 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
    14881 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
    14941 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
    15001 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
    15061 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
    15121 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
    15181 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
    15241 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
    15301 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
    15361 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
    15421 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
    15481 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
    15541 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
    15601 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
    15661 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
    15721 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
    15781 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
    15841 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
    15901 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
    15961 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
    16021 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
    16081 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
    16141 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
    16201 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
    16261 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
    16321 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
    16381 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
    16441 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
    16501 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
    16561 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
    16621 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
    16681 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
    16741 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
    16801 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc ac
//
