LOCUS       sequence-441803-       11082 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mbr-1 fluorescent neural reporter driving nuclear mNeptune2.5 and 
            mTagBF2 expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11082)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 11082)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY50
FEATURES             Location/Qualifiers
     source          1..11082
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     regulatory      2002..2011
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5373..5393
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5418..5438
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5460..5480
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6384..6437
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6447..6467
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6489..6509
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(8673..8695)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8795..8814
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8892..8910)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8978..9082
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9083..9151,9152..9943)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9301..9320)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10114..10702
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10603..10622
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10856..10873
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10990..11011
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11026..11031,11032..11049,11050..11056)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11064..11080
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(11069..11082,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagt gcaccctgct
      181 catactctca cttttacata ataagtatta ataattaatg agttcggagg gcgaaagagt
      241 aagattaagt gctagatttg atgttttcca agctttgggg aaaaacgggg gcaggagcgc
      301 atcctttagg cggctcaaga aaaggtgaat cttttcatgt cttcttttca ctctgagaaa
      361 cacttttgtg agtggtgtgt ttttgacatt tctaaaattt catacatttt tacacttttg
      421 cctgtcacca ccatactggt ccttacgccc tttgtgcaac tcgcctttca aacccactgg
      481 acccttccaa aatgatcgga tcttattgga ttctggaaat tatttcgatt cttttttatg
      541 aatatttcac catttttgag aaaattttga aatctgaaaa aattggatct ccggaaaaaa
      601 atatgtgaga aaatttgaaa ctttagagtt tctcacgacc tattctggac ggaggaatgg
      661 attttaaatt tttatgtact tatgcaaaaa ttcaggtctc tttttgaaac tggtacattt
      721 tatttttgtt ttactttttt cgatgctcat ttatttgttt tctttctagt agtaaacttt
      781 aaaaagccga tgagaaaagg gggaaaatgt attgtatgtt ttagcaaaaa aattaaatat
      841 tacctatttt taattctcaa attactattt ccgatgaaaa tcttcagaga gcctcaaaat
      901 ttggacggag aagtaagtcc attttagcgt aattttaaaa actgaaaagt tctacattga
      961 agttttttaa ataacttctg aagtatttag aaattaggtg ttatcaactg aaattttgca
     1021 atatttaaat agaacttgtt atggcaaaaa tatactcata tgaacctatg tcaacgacct
     1081 aataatatga aaaactgatt tttcaaactt ttaccttgaa aacaaattca cagcgtttgc
     1141 aaaaaccaaa tactacagca ttttagtgat cctctaccag aaatacaaaa aggtgggtgg
     1201 agctaaaaca gtgattggga tggtttctac gatccatctt tccatttatc attttgttca
     1261 cccaccacag tctgaatcat cacgtttgca cagatacgcg ctggctgtta cagtaccttc
     1321 cagcaccatt tgtcccgtgt cgtaataatt tcaattgtga ttcacgaatt tccatcaaat
     1381 cttcctacca ggttttcagt tttttaagca tcaagcaagc tcattcatta ttttcgagaa
     1441 gcactgcttt cattttccaa aagcaatttg cgacgcgcta agacccaaaa gtaacgaatt
     1501 tgattgaatt caaagccgtc ccgaatcatc gtttctcccc cttttcttgc ccccgtcttc
     1561 ctttttcgtt ttcccccgtg tgctccgcct cctatgaaaa ttcgtttcct aattagaaga
     1621 ttagcagcca cggatgcttt tgaatgcaat tctgccgtgt agccacaggc ggtgcatgat
     1681 ttagtcgttt tcttcatttg gcgaatgaaa aaatacgttg ttaccgtatg atattcctgc
     1741 agatccatat ttttgagagt accacccctt gttccttcaa ccccggcttt tgtgaaaatt
     1801 ctgaattaaa tatcactatg catgtcttct tatttccatt ctcttctaaa cttgtacata
     1861 ttttgaagaa aatgaaaaag agtgagagaa gaagtagtaa gaaaacgaaa attaaagtat
     1921 atcaggcata ttcacatgta tttcgcgagg tgtatggatg tgtccataat ttatcagaca
     1981 tggaattatg aaacgaagag cccatggcgg cctaatgaga ttgaattttg gtttggcgca
     2041 ttctaaatgt ttttgtcacc agattcttca cagtattacc tagccttcat cttccaaaac
     2101 tgtcatttta aaaataacaa gttacgatct ttgccaattt caaatcgatt caaaaagaaa
     2161 agatgaacta aaagaccagg ggcgatttta ttgaacaatc gtcagaataa aatgaatgtt
     2221 tttgtttcag aggatactgt agcttttttt gcagccgatt tgtgattttt tctatgaagt
     2281 tgattgttga gacgccaata tcctaaatct ggattcaact aaaatgcatt atttgatgta
     2341 ggacatgtta cattactttg aaaatcataa tcgaaattgc atatctgtag tcttttatct
     2401 atgtgcattg taaaagtatt gattccattt tttagacaaa cataaagtat caaagcgtta
     2461 cggcttttgt atttcaagac cgcattttcc tctaaaataa tatttcatgc aattcaatgg
     2521 actatgttga gtaagacaaa aattcctctt ttatgaattc gcagataccg aaaccgttgt
     2581 aagttcgctt agaaattgta atagtcatct gcaggagttt gttaaaaggc taaatatata
     2641 acggttagta agaaactgct agagtgtgaa cgttttccct tttgccgagc cggtcgtttt
     2701 tttctttgct aaaattggct aaaattgctt atcaggtaac atgtactttt tacgaacatc
     2761 gaaggaattt tagagtctgc cacaaaacaa ctagtattaa taatgacttt ttgctcgtta
     2821 tgtgcctaaa caatttgcga gtaatgaaac agttttctag tttcatccgt cttgatctat
     2881 ttcctttagt tccgtccttc ttttatttgt ttgtttttcc atacttgtcc ctctggtcct
     2941 tacctcatca gctctcgtct tttcgtcgcg ctgttttttt cggttcgttc aaattgtttt
     3001 aaattttggc gaatccttaa ttttcgcaga cagaagaggc gagaaaagca aaaaaattcg
     3061 aagatgcgca caattgattc tggaatttgg gtgagagagg tggggcgcca attatatctc
     3121 gcgtattgca catagggggc gagatgggaa atcgccgggt ttgctctaat taaaaacaag
     3181 actttcagga aacggcagtg ttacgtttta aaaactaacc aaaccagccc actgggaagg
     3241 ggtttctata tgctacagtg gatatgctac tagcatagga cgaggtagtt ttcccagttt
     3301 cagtttatca aacattgttc ctaatgagtg aaactctgat ttgtatcacc acagtactct
     3361 tatgaccttt atccaattca tttgtgcatt cttgcatcat tggaacattt ttttgtttgt
     3421 tttcatccct tgattacttt ctaagtattg ataggcgacg aagaatgagt aaaataattg
     3481 ccggaactga gtcaggttac tttaactcat ttttccggct tttttgtaag caccgcacaa
     3541 aaaagaaaag taccgccagt tatattcaca ttaacaattt tatgaaaacc caggaccttt
     3601 gttctcgttt tatttgctca ttagacaacg gcacgaactt attgtcaacc aaaatcgagc
     3661 ggggagagca tgttagtata atgtcatggt tttctgttgg gaatagcaaa atttcaagaa
     3721 gagttacgtg aattaagata aaccacacaa acaattagct tgaatgatat tagacgtgac
     3781 agcaacacgg ccaggtagaa atgaatcaga gccaggtatg actagcgtta ctagtagtgt
     3841 aatccttaat attaaatttt tggattatct gtgttacact ttaatttatg tactaaacaa
     3901 aaaatgccac aaggtttttg aatttttggc ttacactcac cccaatcgca tctgttagca
     3961 catcaccttt ctggcgccaa aattcaagtt tttgaatgag tataaacttc cacgaaggtg
     4021 cagatcgaga atgggaatca atatatttac attgtttttg cccgaaaact gattgttttt
     4081 tattagagaa ccaatgagag agatacagta aagtcttgta aattatattg ttttggaaat
     4141 tgagaattaa tggatccttc aaattgattt tataatcatt aaaaaaaaac acattttccc
     4201 agaatcattt agaaaacaaa aatctatctc acaagttcaa ttttccaaaa aactgaaagt
     4261 ggcggttccc accgcggatt tagacactta ggccctcttg gaaacacttt tcggaagtgt
     4321 ttggacactg ttcgataacg atttttatct tctctctaaa aagtgtgttc ggggatttcc
     4381 tgaactttct tcttttaagt ttttcttcca gacaaaaatc atagaataat tgtgaataat
     4441 aacttgtcgt ttggttcaaa agttgaattt gaaaaatttt taccacgatt ttcagaacag
     4501 ttggacaaaa aatttcagct gaaatgcttt ttgcttaaag aaccgtcacg aaaacgagca
     4561 gaaaactcaa ttttgcaaat taaaacatac tttaaccttt gtctatctga aaactagcac
     4621 caggtacata tttcttgttg tactacaaaa cattaatatt aaactttaat atgctccgtt
     4681 tttgaattga aaaactatga gataccttca tttttgatag tttttcaaca tttgtttcat
     4741 ccataacttt aaaactctta tgaccgttca gaacatgttt ctagaactta ttacatttta
     4801 tgagtaatcc cattaaattt ttttcgattt gaaatcaacg ttactttaga tggtctgcaa
     4861 tggttctact ttgaacagat tttcaaaaaa tgaaattagt catatttttt aatagatatt
     4921 ggaaatttta aaaataaagt gtgaaatcag attaatactt ttggcatttt ctgattccaa
     4981 cattcttttg aaaactgata tctgctcaca tagacgttta ttgtttcatt ttcgttacat
     5041 atttttttcc gaaaatttca gttgcctgat gtttttacaa ttctattgtc tcaatttgat
     5101 agggctaatc cttttcttcc gtcaaatttt gatctcctcc attagctcat ttctatgatt
     5161 gtttttcaat aaaattctaa taattctttt aatttacatt tcgtacctca acaaattgta
     5221 ttttcgtagg tacacccggg attggccaaa ggacccaaag gtatgtttcg aatgatacta
     5281 acataacata gaacattttc aggaggaccc ttggagggta ccggttttcc cagtcacgac
     5341 gcgtggtacc gagctcagaa aaaatgaccg ctccaaagaa gaaacgcaaa gtaccgagct
     5401 cagaaaaaat gacggcacct aaaaagaaac gcaaagtacc ggtcgccgag aagacagccc
     5461 caaaaaagaa gcgtaaagtt ccacgcgtca tgggagtaag tttaaacagt tcggtactaa
     5521 ctaaccatac atatttaaat tttcagcgcg ctgtctccaa gggagaggag ctcatcaagg
     5581 agaacatgca caccaagctc tacatggagg gaaccgtcaa caaccaccac ttcaagtgca
     5641 cccacgaagg ggagggcaag ccctacgagg gcacccagac caacaggatt aaggtggtgg
     5701 agggaggccc cctgccgttc gcattcgaca tcctggccac ctgctttatg tacgggagca
     5761 agaccttcat caaccacacc caaggaatcc cagacttctt caagcaatcc ttcgtaagtt
     5821 taaacatgat tttactaact aactaatctg atttaaattt tcagcctgag ggcttcacat
     5881 gggagagagt caccacatac gaagacgggg gcgtgcttac cgttacccag gacaccagcc
     5941 tccaggacgg ctgcttgatc tacaacgtca agctcagagg ggtgaacttc ccatccaacg
     6001 gccctgtgat gcagaagaaa acactcggct gggaggccag taccgagacc ctgtaccccg
     6061 ctgacggcgg cctggaaggc agatgcgaca tggccctgaa gctcgtgggc gggggccacc
     6121 tgcactgcaa cctgaagacc acatacagat ccaagaaacc cgctaagaac ctcaagatgc
     6181 caggagtaag tttaaacata tatatactaa ctaaccctga ttatttaaat tttcaggtct
     6241 actttgtgga ccgcagactg gaaagaatca aggaggccga caatgagacc tacgtcgagc
     6301 agcacgaggt ggctgtggcc agatactgcg acctccctag caaactgggg cacaaactta
     6361 atggcatgga cgagctgtac aaggagggac gtggatccct cctcacctgc ggagacgtcg
     6421 aggagaaccc aggaccaatg accgctccaa agaagaagcg caaggttcca gtcgagaaga
     6481 tgactgcccc aaaaaagaaa cgtaaggtcc cgcggatgtc agagcttatt aaggagaata
     6541 tgcatatgaa actgtacatg gaaggaaccg tggacaacca tcactttaag tgcacttcgg
     6601 agggggaagg caaaccgtac gagggcaccc agaccatgcg aatcaaggtg gtcgagggag
     6661 gcccactgcc cttcgccttc gacattctgg taagtttaaa catatatata ctaactaacc
     6721 ctgattattt aaattttcag gctactagct tcctgtacgg cagcaagact tttattaatc
     6781 acacccaggg catcccggac ttcttcaaac agtcgttccc tgagggcttc acatgggagc
     6841 gtgtgaccac atacgaagac ggtggcgttc tcaccgctac ccaggacacc agcctccagg
     6901 acggttgcct catatataat gtggtaagtt taaacagttc ggtactaact aaccatacat
     6961 atttaaattt tcagaagatc cggggagtaa acttcacatc caatggtccg gttatgcaga
     7021 agaaaacact cggctgggag gccttcaccg agacgctgta tccggctgac ggaggcctgg
     7081 aaggtcgaaa cgacatggcc ctgaagctcg tcggcggtag ccatctcatc gcaaacgcta
     7141 agaccacata tagatccaag aaaccggtaa gtttaaacat gattttacta actaactaat
     7201 ctgatttaaa ttttcaggct aagaacctta aaatgccggg agtctactat gttgactaca
     7261 gactggaacg gatcaaggag gccaacaacg agacctacgt cgagcagcac gaggttgcag
     7321 ttgccagata ctgcgacctc ccgagcaaac tgggtcacaa gcttaatcct aggataccgg
     7381 tgggtgaaga cccaccaaag ccatctgcca agggagccaa gaaggccgcc aagaccgtta
     7441 cgaagccaaa ggacggaaag aagagacgtc atgcccgtaa ggaatcatac tccgtctaca
     7501 tctaccgtgt cctcaagcaa gttcatccag acactggagt ttcctccaaa gccatgtcta
     7561 tcatgaactc ttttgtcaac gatgtcttcg agcgtattgc tgctgaagca tcccgtcttg
     7621 ctcactacaa caagcgttcc acaatctcat cccgcgaaat tcagaccgct gtccgtctga
     7681 tccttccagg agagcttgcc aagcacgccg tgtctgaggg aaccaaggcc gttaccaagt
     7741 acacttccag caagtaggaa ttccaactga gcgccggtcg ctaccattac caacttgtct
     7801 ggtgtcaaaa ataatagggg ccgctgtcat cagagtaagt ttaaactgag ttctactaac
     7861 taacgagtaa tatttaaatt ttcagcatct cgcgcccgtg cctctgactt ctaagtccaa
     7921 ttactcttca acatccctac atgctctttc tccctgtgct cccaccccct atttttgtta
     7981 ttatcaaaaa acttctctta atttctttgt tttttagctt cttttaagtc acctctaaca
     8041 atgaaattgt gtagattcaa aaatagaatt aattcgtaat aaaaagtcga aaaaaattgt
     8101 gctccctccc cccattaata ataattctat cccaaaatct acacaatgtt ctgtgtacac
     8161 ttcttatgtt ttttacttct gataaatttt tttgaaacat catagaaaaa accgcacaca
     8221 aaatacctta tcatatgtta cgtttcagtt tatgaccgca atttttattt cttcgcacgt
     8281 ctgggcctct catgacgtca aatcatgctc atcgtgaaaa agttttggag tatttttgga
     8341 atttttcaat caagtgaaag tttatgaaat taattttcct gcttttgctt tttggggttt
     8401 cccctattgt ttgtcaagat ttcgaggacg gcgtttttct tgctaaaatc acaagtattg
     8461 atgagcacga tgcaagaaag atcggaagaa ggtttgggtt tgaggctcag tggaaggtga
     8521 gtagaagttg ataatttgaa agtggagtag tgtctatggg gtttttgcct taaatgacag
     8581 aatacattcc caatatacca aacataactg tttcctacta gtcggccgta cgggcccttt
     8641 cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
     8701 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
     8761 ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga
     8821 gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
     8881 cggccttaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     8941 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     9001 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     9061 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     9121 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     9181 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     9241 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     9301 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     9361 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     9421 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     9481 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     9541 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     9601 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     9661 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     9721 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     9781 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     9841 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     9901 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     9961 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
    10021 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
    10081 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
    10141 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
    10201 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
    10261 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
    10321 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
    10381 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
    10441 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
    10501 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
    10561 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
    10621 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
    10681 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
    10741 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
    10801 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
    10861 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
    10921 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
    10981 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
    11041 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tt
//
