LOCUS       sequence-441804-        6153 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lin-11(intron 3) fluorescent neural reporter driving nuclear 
            mNeptune2.5 expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6153)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 6153)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY102
FEATURES             Location/Qualifiers
     source          1..6153
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1828..1848
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1873..1893
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1915..1935
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(3744..3766)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3866..3885
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3963..3981)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4049..4153
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4154..4222,4223..5014)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4372..4391)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5185..5773
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5674..5693
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    6061..6082
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6097..6102,6103..6120,6121..6127)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6135..6151
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(6140..6153,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagt ggtgagacga
      181 gcaagagata aagtttttca tattcgatgt tttcaatgct ccgtttgtca aaggttattg
      241 gacactggtg accaggtgag ccaggctaag cctcttctct atataagatt gattgtactt
      301 ttgatcttat atattaatcc aggctggtac cttaattgaa ctaaagtttc aaataagttt
      361 tatattattt aaggctgacg gtaagtgata tttgacgttt taactggcag ttctttttaa
      421 gcttgtctcc accattatca aggtttttct ataagttgag cggtgactca gatgaaatta
      481 gatattcata gtcgctattt tctcacgtct aacttatact gataagccca tttgcaaata
      541 agatacgcag atccctaaaa atcacatgct cacacctctt gaatgtgtcc ccatcatgag
      601 cccctgtcag ttgcacctgc ctcttaccga gagctctcta ccactcaagc ttcacgcctt
      661 cttgtcattc aaaatgtttt gttttcttga atagaagatg tcactgaatg tcattgatgc
      721 tgttctgagt attgttttga ctcatgtaga tgaaacagat agttgattaa aattttgatc
      781 ataaacatta tagggaacat caaaaactat ttctgtctag aggcgcgctg aatatctaaa
      841 aaccctctca acttccatac tgtttgtttt ggtataataa ttctactttt taaaaaagaa
      901 aaattaatgt agaaatatag aaaaaaatat tcgattgact tcaaaataat gaataaatat
      961 tttaaatgga acgtttgaaa aatacttcaa gtactgtttc ggaagttatc aaaactactg
     1021 ttaaagtttt tcagattttt tgaacttttc caaaaaaaaa acactttatt tcagatcaat
     1081 caaatatttt aattttttta aaatttgttt tatttgaaaa atttggttgt cgttgtatag
     1141 ttgccggaag tatttgccgg acaccatact ttaaacattt ctcattatca gttgaaaaac
     1201 aatacgttaa aacaattttc caagaatact aggattttgg agcaaccgtt caaaatgtct
     1261 cgaggcgcat attatcttct cattgctcat tgcttctgct tatattaatt tcaaatcagt
     1321 taaactaccc actgaccaac cggtagatct tatctcaatg ccattttcct tccattttcc
     1381 atcctgtctt cctgcttcaa aaccttcccc aattcccttt gggatgtgtc ctgttgtcac
     1441 tctacaatca tcttctttcc atccgtctct aacctattat actttataat tgcattacac
     1501 gggggaccta gtcaaggttt gagacagctc caactgtcat gaaaatatgc gaggggttcc
     1561 cacgattgtt tttgtttttt tttcgcggaa tttttagtgt tattttgcag ctttatatca
     1621 tggaaggcaa tcgattcgtg tgtcaaagtg attttcaaac agcaaccaaa acatcaacgc
     1681 ccacatcgac ccgggattgg ccaaaggacc caaaggtatg tttcgaatga tactaacata
     1741 acatagaaca ttttcaggag gacccttgga gggtaccggt tttcccagtc acgacgcgtg
     1801 gtaccgagct cagaaaaaat gaccgctcca aagaagaaac gcaaagtacc gagctcagaa
     1861 aaaatgacgg cacctaaaaa gaaacgcaaa gtaccggtcg ccgagaagac agccccaaaa
     1921 aagaagcgta aagttccacg cgtcatggga gtaagtttaa acagttcggt actaactaac
     1981 catacatatt taaattttca gcgcgctgtc tccaagggag aggagctcat caaggagaac
     2041 atgcacacca agctctacat ggagggaacc gtcaacaacc accacttcaa gtgcacccac
     2101 gaaggggagg gcaagcccta cgagggcacc cagaccaaca ggattaaggt ggtggaggga
     2161 ggccccctgc cgttcgcatt cgacatcctg gccacctgct ttatgtacgg gagcaagacc
     2221 ttcatcaacc acacccaagg aatcccagac ttcttcaagc aatccttcgt aagtttaaac
     2281 atgattttac taactaacta atctgattta aattttcagc ctgagggctt cacatgggag
     2341 agagtcacca catacgaaga cgggggcgtg cttaccgtta cccaggacac cagcctccag
     2401 gacggctgct tgatctacaa cgtcaagctc agaggggtga acttcccatc caacggccct
     2461 gtgatgcaga agaaaacact cggctgggag gccagtaccg agaccctgta ccccgctgac
     2521 ggcggcctgg aaggcagatg cgacatggcc ctgaagctcg tgggcggggg ccacctgcac
     2581 tgcaacctga agaccacata cagatccaag aaacccgcta agaacctcaa gatgccagga
     2641 gtaagtttaa acatatatat actaactaac cctgattatt taaattttca ggtctacttt
     2701 gtggaccgca gactggaaag aatcaaggag gccgacaatg agacctacgt cgagcagcac
     2761 gaggtggctg tggccagata ctgcgacctc cctagcaaac tggggcacaa acttaatggc
     2821 atggacgagc tgtacaagta agcgccggtc gctaccatta ccaacttgtc tggtgtcaaa
     2881 aataataggg gccgctgtca tcagagtaag tttaaactga gttctactaa ctaacgagta
     2941 atatttaaat tttcagcatc tcgcgcccgt gcctctgact tctaagtcca attactcttc
     3001 aacatcccta catgctcttt ctccctgtgc tcccaccccc tatttttgtt attatcaaaa
     3061 aacttctctt aatttctttg ttttttagct tcttttaagt cacctctaac aatgaaattg
     3121 tgtagattca aaaatagaat taattcgtaa taaaaagtcg aaaaaaattg tgctccctcc
     3181 ccccattaat aataattcta tcccaaaatc tacacaatgt tctgtgtaca cttcttatgt
     3241 tttttacttc tgataaattt ttttgaaaca tcatagaaaa aaccgcacac aaaatacctt
     3301 atcatatgtt acgtttcagt ttatgaccgc aatttttatt tcttcgcacg tctgggcctc
     3361 tcatgacgtc aaatcatgct catcgtgaaa aagttttgga gtatttttgg aatttttcaa
     3421 tcaagtgaaa gtttatgaaa ttaattttcc tgcttttgct ttttggggtt tcccctattg
     3481 tttgtcaaga tttcgaggac ggcgtttttc ttgctaaaat cacaagtatt gatgagcacg
     3541 atgcaagaaa gatcggaaga aggtttgggt ttgaggctca gtggaaggtg agtagaagtt
     3601 gataatttga aagtggagta gtgtctatgg ggtttttgcc ttaaatgaca gaatacattc
     3661 ccaatatacc aaacataact gtttcctact agtcggccgt acgggccctt tcgtctcgcg
     3721 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
     3781 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
     3841 gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
     3901 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcggccttaa
     3961 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     4021 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     4081 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     4141 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     4201 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     4261 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     4321 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     4381 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     4441 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     4501 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     4561 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     4621 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     4681 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     4741 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     4801 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     4861 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     4921 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     4981 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     5041 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     5101 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     5161 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     5221 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     5281 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     5341 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     5401 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     5461 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     5521 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     5581 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     5641 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     5701 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     5761 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     5821 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     5881 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     5941 cgatgaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     6001 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     6061 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     6121 tatgttgtgt ggaattgtga gcggataaca att
//
