LOCUS       sequence-441805-        7960 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lin-11 fluorescent neural reporter driving nuclear mNeptune2.5 
            expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7960)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 7960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY101
FEATURES             Location/Qualifiers
     source          1..7960
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3635..3655
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3680..3700
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3722..3742
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(5551..5573)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5673..5692
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5770..5788)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5856..5960
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5961..6029,6030..6821)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6179..6198)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6992..7580
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7481..7500
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    7868..7889
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7904..7909,7910..7927,7928..7934)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7942..7958
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(7947..7960,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagc acagcctatt
      181 cttgacaggt atcggtccct cacctttttt tgcaatttat gattattttc cgtttagtct
      241 cattccaatt ttcatctcta gctgctgtaa caccacctct ctcgccccta attgaatttt
      301 tccattttcg attcatttcc ccctctcaac cccttcgaca gcagcagcac tagcagtcat
      361 ttcattttca gccgacactt tcacttactt tcagatatgt attcactgtg ctcggaaaat
      421 gctggcatca gtcttgtctt cgatgttgtg actgtcgagc gcccatgtca atgacttgtt
      481 ttagtagaga tggtcttatt ctgtgtaaaa ctgatttttc aaggtaagtt ccgagcaaca
      541 ttaatttcta agaactgtga tctataaaga gccgggctgc aagttttgag ctcttggaga
      601 tagttcaaaa acatcttaca attaggttgt gatgaatttt ccattgaatt ttccagtgtg
      661 caaactttaa aagttctaac tgaacgctga agttttgaag ctgaaaacct ctaagattct
      721 tcaacagtga caatttcaaa attccaaatt tttagtcaaa agtttatgta aaaatatata
      781 aaacatgatc aatttgaaaa ctacgtttat tttcattgaa aaataaaaaa aagtcctgaa
      841 atctggtatg aaattgtaat aactattttc tggtagatgt caattttcag taagtctctc
      901 agattttttt cagtcttttg caacatattt ttcatattga aaaattggtt tttttcatgc
      961 caattcaaat aatcgtactg cgaatcgtca cccactcctg gatatattgt agttccaaaa
     1021 ctttgccaaa aagggctagg agaatatatt caagcaaatg acggcataaa atttaaagat
     1081 tccgcaattc caaaatcctc ccaaaacatg ttctaaagtt ttgaagatca tattccaaaa
     1141 ttctcaaatt tcttatgctt tcaagctcac ttgtagtaaa caaatcttac ttgcttattt
     1201 tgattttcaa atttcaaatt ctatatctaa attttcccac ggttcccacg gtgtccataa
     1261 ttattttacc tacctgattg tgattttcag gcgatatagt caacggtgtg ccggatgcga
     1321 tgggaaattg gaaaaagagg atctggtgag acgagcaaga gataaagttt ttcatattcg
     1381 atgttttcaa tgctccgttt gtcaaaggtt attggacact ggtgaccagg tgagccaggc
     1441 taagcctctt ctctatataa gattgattgt acttttgatc ttatatatta atccaggctg
     1501 gtaccttaat tgaactaaag tttcaaataa gttttatatt atttaaggct gacggtaagt
     1561 gatatttgac gttttaactg gcagttcttt ttaagcttgt ctccaccatt atcaaggttt
     1621 ttctataagt tgagcggtga ctcagatgaa attagatatt catagtcgct attttctcac
     1681 gtctaactta tactgataag cccatttgca aataagatac gcagatccct aaaaatcaca
     1741 tgctcacacc tcttgaatgt gtccccatca tgagcccctg tcagttgcac ctgcctctta
     1801 ccgagagctc tctaccactc aagcttcacg ccttcttgtc attcaaaatg ttttgttttc
     1861 ttgaatagaa gatgtcactg aatgtcattg atgctgttct gagtattgtt ttgactcatg
     1921 tagatgaaac agatagttga ttaaaatttt gatcataaac attataggga acatcaaaaa
     1981 ctatttctgt ctagaggcgc gctgaatatc taaaaaccct ctcaacttcc atactgtttg
     2041 ttttggtata ataattctac tttttaaaaa agaaaaatta atgtagaaat atagaaaaaa
     2101 atattcgatt gacttcaaaa taatgaataa atattttaaa tggaacgttt gaaaaatact
     2161 tcaagtactg tttcggaagt tatcaaaact actgttaaag tttttcagat tttttgaact
     2221 tttccaaaaa aaaaacactt tatttcagat caatcaaata ttttaatttt tttaaaattt
     2281 gttttatttg aaaaatttgg ttgtcgttgt atagttgccg gaagtatttg ccggacacca
     2341 tactttaaac atttctcatt atcagttgaa aaacaatacg ttaaaacaat tttccaagaa
     2401 tactaggatt ttggagcaac cgttcaaaat gtctcgaggc gcatattatc ttctcattgc
     2461 tcattgcttc tgcttatatt aatttcaaat cagttaaact acccactgac caaccggtag
     2521 atcttatctc aatgccattt tccttccatt ttccatcctg tcttcctgct tcaaaacctt
     2581 ccccaattcc ctttgggatg tgtcctgttg tcactctaca atcatcttct ttccatccgt
     2641 ctctaaccta ttatacttta taattgcatt acacggggga cctagtcaag gtttgagaca
     2701 gctccaactg tcatgaaaat atgcgagggg ttcccacgat tgtttttgtt tttttttcgc
     2761 ggaattttta gtgttatttt gcagctttat atcatggaag gcaatcgatt cgtgtgtcaa
     2821 agtgattttc aaacagcaac caaaacatca acgcccacat cgattcatag gcccggtaag
     2881 gtttttaaaa ataaagaaag ggaaagagaa agagacgaag aattgagttt taaagataat
     2941 acaaaagttg ttcaaagtta tggtagaagg tggagttttc ataaaattgt tgactgctga
     3001 ggcgtccaag gcctactcca ataattgaat gaaggcagat aggctagagg cagacaagtg
     3061 agcctgaaca catgcctacc taccatgaaa ttctttatct ctgaagtaac accgccagac
     3121 ataattttca gtagataccc aaaaagtcaa aaaagaattc ccagatttca agtcataaaa
     3181 tccttagaaa tttcttcttc ttctcataaa accagagaca tacgttctaa tccatgcttc
     3241 cgtttcaaac gtatttaaga tgagccagcg agccatgtgt tgtttgaata gttttacgat
     3301 gataggtatc tctgcacaaa agaggaagag acagacagct gtcaacacag aaaacatttc
     3361 tttgtattcg aattaccgca aaatagtaag gaagggggga taatctcgtt gcggaatgga
     3421 catacactgg tattagacag atgatgttct tttgttacag tatccaatgg atccgaatgc
     3481 aattccgatg tggaggcccg ggattggcca aaggacccaa aggtatgttt cgaatgatac
     3541 taacataaca tagaacattt tcaggaggac ccttggaggg taccggtttt cccagtcacg
     3601 acgcgtggta ccgagctcag aaaaaatgac cgctccaaag aagaaacgca aagtaccgag
     3661 ctcagaaaaa atgacggcac ctaaaaagaa acgcaaagta ccggtcgccg agaagacagc
     3721 cccaaaaaag aagcgtaaag ttccacgcgt catgggagta agtttaaaca gttcggtact
     3781 aactaaccat acatatttaa attttcagcg cgctgtctcc aagggagagg agctcatcaa
     3841 ggagaacatg cacaccaagc tctacatgga gggaaccgtc aacaaccacc acttcaagtg
     3901 cacccacgaa ggggagggca agccctacga gggcacccag accaacagga ttaaggtggt
     3961 ggagggaggc cccctgccgt tcgcattcga catcctggcc acctgcttta tgtacgggag
     4021 caagaccttc atcaaccaca cccaaggaat cccagacttc ttcaagcaat ccttcgtaag
     4081 tttaaacatg attttactaa ctaactaatc tgatttaaat tttcagcctg agggcttcac
     4141 atgggagaga gtcaccacat acgaagacgg gggcgtgctt accgttaccc aggacaccag
     4201 cctccaggac ggctgcttga tctacaacgt caagctcaga ggggtgaact tcccatccaa
     4261 cggccctgtg atgcagaaga aaacactcgg ctgggaggcc agtaccgaga ccctgtaccc
     4321 cgctgacggc ggcctggaag gcagatgcga catggccctg aagctcgtgg gcgggggcca
     4381 cctgcactgc aacctgaaga ccacatacag atccaagaaa cccgctaaga acctcaagat
     4441 gccaggagta agtttaaaca tatatatact aactaaccct gattatttaa attttcaggt
     4501 ctactttgtg gaccgcagac tggaaagaat caaggaggcc gacaatgaga cctacgtcga
     4561 gcagcacgag gtggctgtgg ccagatactg cgacctccct agcaaactgg ggcacaaact
     4621 taatggcatg gacgagctgt acaagtaagc gccggtcgct accattacca acttgtctgg
     4681 tgtcaaaaat aataggggcc gctgtcatca gagtaagttt aaactgagtt ctactaacta
     4741 acgagtaata tttaaatttt cagcatctcg cgcccgtgcc tctgacttct aagtccaatt
     4801 actcttcaac atccctacat gctctttctc cctgtgctcc caccccctat ttttgttatt
     4861 atcaaaaaac ttctcttaat ttctttgttt tttagcttct tttaagtcac ctctaacaat
     4921 gaaattgtgt agattcaaaa atagaattaa ttcgtaataa aaagtcgaaa aaaattgtgc
     4981 tccctccccc cattaataat aattctatcc caaaatctac acaatgttct gtgtacactt
     5041 cttatgtttt ttacttctga taaatttttt tgaaacatca tagaaaaaac cgcacacaaa
     5101 ataccttatc atatgttacg tttcagttta tgaccgcaat ttttatttct tcgcacgtct
     5161 gggcctctca tgacgtcaaa tcatgctcat cgtgaaaaag ttttggagta tttttggaat
     5221 ttttcaatca agtgaaagtt tatgaaatta attttcctgc ttttgctttt tggggtttcc
     5281 cctattgttt gtcaagattt cgaggacggc gtttttcttg ctaaaatcac aagtattgat
     5341 gagcacgatg caagaaagat cggaagaagg tttgggtttg aggctcagtg gaaggtgagt
     5401 agaagttgat aatttgaaag tggagtagtg tctatggggt ttttgcctta aatgacagaa
     5461 tacattccca atataccaaa cataactgtt tcctactagt cggccgtacg ggccctttcg
     5521 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
     5581 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
     5641 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt
     5701 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
     5761 gccttaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     5821 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     5881 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     5941 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     6001 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     6061 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     6121 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     6181 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     6241 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     6301 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     6361 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     6421 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     6481 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     6541 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     6601 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     6661 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     6721 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     6781 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     6841 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     6901 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     6961 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     7021 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     7081 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     7141 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     7201 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     7261 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     7321 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     7381 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     7441 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     7501 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     7561 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     7621 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     7681 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     7741 cagtgagcga tgaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     7801 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     7861 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     7921 cggctcgtat gttgtgtgga attgtgagcg gataacaatt
//
