LOCUS       sequence-441806-       17080 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses a heat-shock inducible cre::EBD fusion protein and 
            UAS-dBrainbow.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17080)
  TITLE     Multigenic Drosophila vectors and transgenic lines for 
            recombinase-directed fluorescent labeling and lineage tracing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 17080)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: hs-cre::EBD, UAS-dBrainbow attB
FEATURES             Location/Qualifiers
     source          1..17080
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      4..432
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(91..110)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     301..322
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     623..642
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(675..693)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(1145..1216,1618..1891,1966..2620,2682..2997,3201..3332,
                     3403..4017)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    4800..4894
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        4913..5151
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     protein_bind    5244..5277
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    5299..5332
                     /label=lox5171
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTGTAC) (Shaw et al., 2021). lox5171 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    5354..5387
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     intron          5527..5592
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5722..5742
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    6014..6148
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    6971..7004
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     CDS             7016..7729
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(7046..7074)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     7669..7689
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             7730..7762
                     /codon_start=1
                     /product="HSV (herpes simplex virus) epitope tag"
                     /label=HSV tag
                     /translation="QPELAPEDPED"
     protein_bind    7782..7815
                     /label=lox5171
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTGTAC) (Shaw et al., 2021). lox5171 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     CDS             7820..8527
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
     CDS             8528..8554
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             8558..8584
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             8588..8614
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    8640..8673
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             9395..9436
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     intron          9542..9607
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9737..9757
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    10029..10163
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             10824..11846
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="SNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLL
                     SVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLP
                     RPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFL
                     GIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVER
                     WISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQR
                     YLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLED
                     G"
     intron          12829..12894
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             13024..13044
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    13316..13450
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    13466..13560
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        13579..13817
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     intron          13954..14019
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             14149..14169
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    14441..14575
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    14604..14673
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(14892..14910)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(14931..14947)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14931..14947)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(14944..14966)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    14955..14971
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(14979..14985,14986..15003,15004..15009))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    15024..15045
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(15162..15179)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(15333..15921)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(15413..15432)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(16092..16883,16884..16952))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     16715..16734
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(16953..17057)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
       61 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
      121 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
      181 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
      241 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
      301 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
      361 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
      421 ttaacgcgaa ttttaacaaa atattaacgc ttacaaktyc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtacgtaccg
      661 ggcccctagt atgtatgtaa gttaataaaa cccatttttg cggaaagtag ataaaaaaaa
      721 catttttttt ttttactgca ctggatatca ttgaacttat ctgatcagtt ttaaatttac
      781 ttcgatccaa gggtatttga tgtaccaggt tctttcgatt acctctcact caaaatgaca
      841 ttccactcaa agtcagcgct gtttgcctcc ttctctgtcc acagaaatat cgccgtctct
      901 ttcgccgctg cgtccgctat ctctttcgcc accgtttgta gcgttacgta gcgtcaatgt
      961 ccgccttcag ttgcattttg tcagcggttt cgtgacgaag ctccaagcgg tttacgccat
     1021 caattaaaca caaagtgctg tgccaaaact cctctcgctt cttatttttg tttgtttttt
     1081 gagtgattgg ggtggtgatt ggttttgggt gggtaagcag gggaaagtgt gaaaaatccc
     1141 ggcaatgggc caagaggatc aggagctatt aattcgcgga ggcagcaaac acccatctgc
     1201 cgagcatctg aacaatgtga gtagtacatg tgcatacatc ttaagttcac ttgatctata
     1261 ggaactgcga ttgcaacatc aaattgtctg cggcgtgaga actgcgaccc acaaaaatcc
     1321 caaaccgcaa ttgcacaaac aaatagtgac acgaaacaga ttattctggt agctgttctc
     1381 gctatataag acaatttttg agatcatatc atgatcaaga catctaaagg cattcatttt
     1441 cgactatatt cttttttaca aaaaatataa caaccagata ttttaagctg atcctagatg
     1501 cacaaaaaat aaataaaagt ataaacctac ttcgtaggat acttcggggt actttttgtt
     1561 cggggttaga tgagcataac gcttgtagtt gatatttgag atcccctatc attgcagggt
     1621 gacagcggag cggcttcgca gagctgcatt aaccagggct tcgggcaggc caaaaactac
     1681 ggcacgctcc ggccacccag tccgccggag gactccggtt cagggagcgg ccaactagcc
     1741 gagaacctca cctatgcctg gcacaatatg gacatctttg gggcggtcaa tcagccgggc
     1801 tccggatggc ggcagctggt caaccggaca cgcggactat tctgcaacga gcgacacata
     1861 ccggcgccca ggaaacattt gctcaagaac ggtgagtttc tattcgcagt cggctgatct
     1921 gtgtgaaatc ttaataaagg gtccaattac caatttgaaa ctcagtttgc ggcgtggcct
     1981 atccgggcga acttttggcc gtgatgggca gttccggtgc cggaaagacg accctgctga
     2041 atgcccttgc ctttcgatcg ccgcagggca tccaagtatc gccatccggg atgcgactgc
     2101 tcaatggcca acctgtggac gccaaggaga tgcaggccag gtgcgcctat gtccagcagg
     2161 atgacctctt tatcggctcc ctaacggcca gggaacacct gattttccaa gccatggtgc
     2221 ggatgccacg acatctgacc tatcggcagc gagtggcccg cgtggatcag gtgatccagg
     2281 agctttcgct cagcaaatgt cagcacacga tcatcggtgt gcccggcagg gtgaaaggtc
     2341 tgtccggcgg agaaaggaag cgtctggcat tcgcctccga ggctctaacc gatccgccgc
     2401 ttctgatctg cgatgagccc acctccggac tggactcctt taccgcccac agcgtcgtcc
     2461 aggtgctgaa gaagctgtcg cagaagggca agaccgtcat cctgaccatt catcagccgt
     2521 cttccgagct gtttgagctc tttgacaaga tccttctgat ggccgagggc agggtagctt
     2581 tcttgggcac tcccagcgaa gccgtcgact tcttttccta gtgagttcga tgtgtttatt
     2641 aagggtatct agtattacat aacatctcaa ctcctatcca gcgtgggtgc ccagtgtcct
     2701 accaactaca atccggcgga cttttacgta caggtgttgg ccgttgtgcc cggacgggag
     2761 atcgagtccc gtgatcggat cgccaagata tgcgacaatt ttgccattag caaagtagcc
     2821 cgggatatgg agcagttgtt ggccaccaaa aatctggaga agccactgga gcagccggag
     2881 aatgggtaca cctacaaggc cacctggttc atgcagttcc gggcggtcct gtggcgatcc
     2941 tggctgtcgg tgctcaagga accactcctc gtaaaagtgc gacttattca gacaacggtg
     3001 agtggttcca gtggaaacaa atgatataac gcttacaatt cttggaaaca aattcgctag
     3061 attttagata gaattgcctg attccacacc cttcttagtt tttttcaatg agatgtatag
     3121 tttatagttt tgcagaagat aaataaattt catttaactc gcgaatatta atgagatgcg
     3181 agtaacattt taatttgcag atggttgcca tcttgattgg cctcatcttt ttgggccaac
     3241 aactcacgca agtgggtgtg atgaatatca acggagccat cttcctcttc ctgaccaaca
     3301 tgacctttca aaacgtcttt gccacgataa atgtaagtca tgtttagaat acatttgcat
     3361 ttcaataatt tactaacttt ctaatgaatc gattcgattt aggtgttcac ctcagagctg
     3421 ccagttttta tgagggaggc ccgaagtcga ctttatcgct gtgacacata ctttctgggc
     3481 aaaacgattg ccgaattgcc gctttttctc acagtgccac tggtcttcac ggcgattgcc
     3541 tatccgatga tcggactgcg ggccggagtg ctgcacttct tcaactgcct ggcgctggtc
     3601 actctggtgg ccaatgtgtc aacgtccttc ggatatctaa tatcctgcgc cagctcctcg
     3661 acctcgatgg cgctgtctgt gggtccgccg gttatcatac cattcctgct ctttggcggc
     3721 ttcttcttga actcgggctc ggtgccagta tacctcaaat ggttgtcgta cctctcatgg
     3781 ttccgttacg ccaacgaggg tctgctgatt aaccaatggg cggacgtgga gccgggcgaa
     3841 attagctgca catcgtcgaa caccacgtgc cccagttcgg gcaaggtcat cctggagacg
     3901 cttaacttct ccgccgccga tctgccgctg gactacgtgg gtctggccat tctcatcgtg
     3961 agcttccggg tgctcgcata tctggctcta agacttcggg cccgacgcaa ggagtagccg
     4021 acatatatcc gaaataactg cttgtttttt ttttttacca ttattaccat cgtgtttact
     4081 gtttattgcc ccctcaaaaa gctaatgtaa ttatatttgt gccaataaaa acaagatatg
     4141 acctatagaa tacaagtatt tccccttcga acatccccac aagtagactt tggatttgtc
     4201 ttctaaccaa aagacttaca cacctgcata ccttacatca aaaactcgtt tatcgctaca
     4261 taaaacaccg ggatatattt tttatataca tacttttcaa atcgcgcgcc ctcttcataa
     4321 ttcacctcca ccacaccacg tttcgtagtt gctctttcgc tgtctcccac ccgctctccg
     4381 caacacattc accttttgtt cgacgacctt ggagcgactg tcgttagttc cgcgcgattc
     4441 ggttcgctca aatggttccg agtggttcat ttcgtctcaa tagaaattag taataaatat
     4501 ttgtatgtac aatttatttg ctccaatata tttgtatata tttccctcac agctatattt
     4561 attctaattt aatattatga ctttttaagg taattttttg tgacctgttc ggagtgatta
     4621 gcgttacaat ttgaactgaa agtgacatcc agtgtttgtt ccttgtgtag atgcatctca
     4681 aaaaaatggt gggcataata gtgttgttta tatatatcaa aaataacaac tataataata
     4741 agaatacatt taatttagaa aatgcttgga tttcactgga actaggctag cctgcaggtc
     4801 ggagtactgt cctccgagcg gagtactgtc ctccgagcgg agtactgtcc tccgagcgga
     4861 gtactgtcct ccgagcggag tactgtcctc cgagcggaga ctctagcgag cgccggagta
     4921 taaatagagg cgcttcgtct acggagcgac aattcaattc aaacaagcaa agtgaacacg
     4981 tcgctaagcg aaagctaagc aaataaacaa gcgcagctga acaagctaaa caatctgcag
     5041 taaagtgcaa gttaaagtga atcaattaaa agtaaccagc aaccaagtaa atcaactgca
     5101 actactgaaa tctgccaaga agtaattatt gaatacaaga agagaactct gaatagggaa
     5161 ttgggaattg gccggccact gcaactactg aaatctgcca agaagtaatt attgaataca
     5221 agaagagaac tctgaataga tctataactt cgtataaagt atcctatacg aagttatgag
     5281 gcagcgcggc tatcgtggat aacttcgtat aatgtgtact atacgaagtt atctggccac
     5341 gacgggcgtt cctataactt cgtataatgt atgctatacg aagttatgcc ctgaatgcgg
     5401 ccgccagtgt gatggatatc tgcagaattc gcccttcggc cgccaaaatg gatctttgtg
     5461 aaggaacctt acttctgtgg tgtgacataa ttggacaaac tacctacaga gatttaaagc
     5521 tctaaggtaa atataaaatt tttaagtgta taatgtgtta aactactgat tctaattgtt
     5581 tgtgtatttt agattccaac ctatggaact gatgaatggg agcagtggtg gaatgccttt
     5641 aatgaggaaa acctgttttg ctcagaagaa atgccatcta gtgatgatga ggctactgct
     5701 gactctcaac attctactcc tccaaaaaag aagagaaagg tagaagaccc caaggacttt
     5761 ccttcagaat tgctaagttt tttgagtcat gctgtgttta gtaatagaac tcttgcttgc
     5821 tttgctattt acaccacaaa ggaaaaagct gcactgctat acaagaaaat tatggaaaaa
     5881 tatttgatgt atagtgcctt gactagagat cataatcagc cataccacat ttgtagaggt
     5941 tttacttgct ttaaaaaacc tcccacacct ccctctgaac ctgaaacata aaatgaatgc
     6001 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     6061 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     6121 catcaatgta tcttatcatg tctggatctg cgtcgcgcca cttcaacgct cgatgggagc
     6181 gtcattggtg ggcggggtaa ccgtcgaaat cagtgtttac gcttccaatc gcaacaaaaa
     6241 attcactgca acactgaaaa gcatacgaaa acgatgaaga ttgtacgagg aaccataaag
     6301 tattttatcc acaaagacac gtatagcaga aaagccaagt taactcggcg ataagttgtg
     6361 tacacaagaa taaaatcggc cagattcagt gttgtcagaa ataagaaaac cccactatgt
     6421 ttttctttgc cttttctttc tcccagcgat cattcatttc gtggtgaaag aacggggtca
     6481 ttgcacggag tttcgactgc gggaaagcag agctgccgtt cacttcgtct ataattagcg
     6541 ctttctattt tccccgattc gggccgctgc tgcgcttttc cgcctgctgt ttgtggcaag
     6601 tgtagcagca ggctgtgcac gcagtgtggc atgcacttgg ctttccaccg ttggtatcga
     6661 ttctctggga cgatgagtca ttcctttcgg ggccacagca taatcgttgc cagctcaccg
     6721 aaatggtgac ttcatttctt aactgccgtc aagcatgcga ttgtacatac atacatattt
     6781 atatatgtac atatttatgt gactatggta ggtcgatata atagcaatca acgcaagcaa
     6841 atgtgtcagt cctgcttaca ggaacgattc tatttagtaa ttttcgttgt ataaagtaat
     6901 tatgtatgta tgtaagcccc ataaatctga aacaattagg caaaaccatg cgaggatgaa
     6961 ctgcaagacg ataacttcgt ataaagtatc ctatacgaag ttatgcggcc gcaaaatgag
     7021 taaaggagaa gaacttttca ctggagttgt cccaattctt gttgaattag atggtgatgt
     7081 taatgggcac aaattttctg tcagtggaga gggtgaaggt gatgcaacat acggaaaact
     7141 tacccttaaa tttatttgca ctactggaaa actacctgtt ccatggccaa cacttgtcac
     7201 tactttaact tatggtgttc aatgcttttc aagataccca gatcatatga aacagcatga
     7261 ctttttcaag agtgccatgc ccgaaggtta tgtccaggaa agaactatat ttttcaaaga
     7321 tgacgggaac tacaagacac gtgctgaagt caagtttgaa ggtgataccc ttgttaatag
     7381 aatcgagtta aaaggtattg attttaaaga agatggaaac attcttggac acaaattgga
     7441 atacaactat aactcacaca atgtatacat catggcagac aaacaaaaga atggaatcaa
     7501 agttaacttc aaaattagac acaacattga agatggaagc gttcaactag cagaccatta
     7561 tcaacaaaat actccaattg gcgatggccc tgtcctttta ccagacaacc attacctgtc
     7621 cacacaatct gccctttcga aagatcccaa cgaaaagaga gaccacatgg tccttcttga
     7681 gtttgtaaca gctgctggga ttacacatgg catggatgaa ctatacaaac agcccgagct
     7741 ggcccccgag gatcccgagg attgacgtaa gctagctcga gataacttcg tataatgtgt
     7801 actatacgaa gttatcaaaa tggtgagcaa gggcgaggag ctcacaatgg gcgtaatcaa
     7861 gcccgacatg aagatcaagc tgaagatgga gggcaacgtg aatggccacg ccttcgtgat
     7921 cgagggcgag ggcgagggca agccctacga cggcaccaac accatcaacc tggaggtgaa
     7981 ggagggagcc cccctgccct tctcctacga cattctgacc accgcgttcg cctacggcaa
     8041 cagggccttc accaagtacc ccgacgacat ccccaactac ttcaagcagt ccttccccga
     8101 gggctactct tgggagcgca ccatgacctt cgaggacaag ggcatcgtga aggtgaagtc
     8161 cgacatctcc atggaggagg actccttcat ctacgagata cacctcaagg gcgagaactt
     8221 cccccccaac ggccccgtga tgcagaagaa gaccaccggc tgggacgcct ccaccgagag
     8281 gatgtacgtg cgcgacggcg tgctgaaggg cgacgtcaag cacaagctgc tgctggaggg
     8341 cggcggccac caccgcgttg acttcaagac catctacagg gccaagaagg cggtgaagct
     8401 gcccgactat cactttgtgg accaccgcat cgagatcctg aaccacgaca aggactacaa
     8461 caaggtgacc gtttacgaga gcgccgtggc ccgcaactcc accgacggca tggacgagct
     8521 gtacaagtac ccatacgatg ttcctgacta tgcgggctat ccctatgacg tcccggacta
     8581 tgcaggatat ccatatgacg ttccagatta cgctggcgga taacgtaagc tagggtacca
     8641 taacttcgta taatgtatgc tatacgaagt tatcaaaatg ataccggtcg gagtaagcaa
     8701 aggtgaggag agcgtgatca aacccgagat gaagatgcgc tactatatgg acggcagcgt
     8761 caacggccac gagtttacca tcgagggcga gggcacggga cgcccgtacg aaggacacca
     8821 ggagatgacc ctgcgcgtga ccatggccga gggtggcccg atgcccttcg ccttcgatct
     8881 ggtgagccac gtgttctgct acggtcaccg cgtcttcacc aaataccccg aggaaatccc
     8941 cgattacttc aagcaagcct tcccggaggg cctgtcgtgg gagcgcagcc tggagttcga
     9001 ggatggcgga agcgccagcg tgagcgcgca catcagcctg cggggcaaca ccttctacca
     9061 taagagcaag ttcaccggcg tgaatttccc agccgatggc cccatcatgc agaaccagag
     9121 tgtggattgg gagcccagca ccgagaagat caccgcaagc gatggcgtgc tgaagggcga
     9181 tgttaccatg tacctgaagc tggagggagg cggcaaccac aagtgccaga tgaaaaccac
     9241 ctacaaggcc gccaagggga tcctggagat gcccggagat cactacattg gccaccgcct
     9301 ggtccgcaag acagaaggaa acatcaccga gcaggtggag gatgccgtgg cgcattcggg
     9361 catggatgag ctgtacaagg gcgcgccagc taatggcaag cccatcccca accccctgct
     9421 gggcctggat agcacctgac ttaattaact gaagacgtcc tagaggatct ttgtgaagga
     9481 accttacttc tgtggtgtga cataattgga caaactacct acagagattt aaagctctaa
     9541 ggtaaatata aaatttttaa gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt
     9601 attttagatt ccaacctatg gaactgatga atgggagcag tggtggaatg cctttaatga
     9661 ggaaaacctg ttttgctcag aagaaatgcc atctagtgat gatgaggcta ctgctgactc
     9721 tcaacattct actcctccaa aaaagaagag aaaggtagaa gaccccaagg actttccttc
     9781 agaattgcta agttttttga gtcatgctgt gtttagtaat agaactcttg cttgctttgc
     9841 tatttacacc acaaaggaaa aagctgcact gctatacaag aaaattatgg aaaaatattt
     9901 gatgtatagt gccttgacta gagatcataa tcagccatac cacatttgta gaggttttac
     9961 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
    10021 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
    10081 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
    10141 atgtatctta tcatgtctgg atccaagctt ctagaatccc aaaacaaact agttgttgtg
    10201 gtaggtcatt tgtttggcag aaagaaaact cgagaaattt ctctggccgt tattcgttat
    10261 tctctctttt ctttttgggt ctctccctct ctgcactaat gctctctcac tctgtcacac
    10321 agtaaacggc atactgctct cgttggttcg agagagcgcg cctcgaatgt tcgcgaaaag
    10381 agcgccggag tataaataga ggcgcttcgt ctacggagcg acaattcaat tcaaacaagc
    10441 aaagtgaaca cgtcgctaag cgaaagctaa gcaaataaac aagcgcagct gaacaagcta
    10501 aacaatctgc agtaaagtgc aagttaaagt gaatcaatta aaagtaacca gcaaccaagt
    10561 aaatcaactg caactactga aatctgccaa gaagtaatta ttgaatacaa gaagagggcg
    10621 cgccatatgc cgtacgaaac cattatctga gtgttaaatg gtaggcatca cacacgatta
    10681 acaaccccta aaaatacact ttgaaaatat tgaaaatatg ggtacctcta gtctcgagtt
    10741 tgtatacatt tttgatattt tcaaacaata cgcagttata aaactcatta gctaacccat
    10801 tttttctttg cttatgctta cagtccaatt tactgaccgt acaccaaaat ttgcctgcat
    10861 taccggtcga tgcaacgagt gatgaggttc gcaagaacct gatggacatg ttcagggatc
    10921 gccaggcgtt ttctgagcat acctggaaaa tgcttctgtc cgtttgccgg tcgtgggcgg
    10981 catggtgcaa gttgaataac cggaaatggt ttcccgcaga acctgaagat gttcgcgatt
    11041 atcttctata tcttcaggcg cgcggtctgg cagtaaaaac tatccagcaa catttgggcc
    11101 agctaaacat gcttcatcgt cggtccgggc tgccacgacc aagtgacagc aatgctgttt
    11161 cactggttat gcggcggatc cgaaaagaaa acgttgatgc cggtgaacgt gcaaaacagg
    11221 ctctagcgtt cgaacgcact gatttcgacc aggttcgttc actcatggaa aatagcgatc
    11281 gctgccagga tatacgtaat ctggcatttc tggggattgc ttataacacc ctgttacgta
    11341 tagccgaaat tgccaggatc agggttaaag atatctcacg tactgacggt gggagaatgt
    11401 taatccatat tggcagaacg aaaacgctgg ttagcaccgc aggtgtagag aaggcactta
    11461 gcctgggggt aactaaactg gtcgagcgat ggatttccgt ctctggtgta gctgatgatc
    11521 cgaataacta cctgttttgc cgggtcagaa aaaatggtgt tgccgcgcca tctgccacca
    11581 gccagctatc aactcgcgcc ctggaaggga tttttgaagc aactcatcga ttgatttacg
    11641 gcgctaagga tgactctggt cagagatacc tggcctggtc tggacacagt gcccgtgtcg
    11701 gagccgcgcg agatatggcc cgcgctggag tttcaatacc ggagatcatg caagctggtg
    11761 gctggaccaa tgtaaatatt gtcatgaact atatccgtaa cctggatagt gaaacagggg
    11821 caatggtgcg cctgctggaa gatggcgagc ggccgcacag cctggccttg tccctgacgg
    11881 ccgaccagat ggtcagtgcc ttgttggatg ctgagccccc catactctat tccgagtatg
    11941 atcctaccag acccttcagt gaagcttcga tgatgggctt actgaccaac ctggcagaca
    12001 gggagctggt tcacatgatc aactgggcga agagggtgcc aggctttgtg gatttgaccc
    12061 tccatgatca ggtccacctt ctagaatgtg cctggctaga gatcctgatg attggtctcg
    12121 tctggcgctc catggagcac ccagggaagc tactgtttgc tcctaacttg ctcttggaca
    12181 ggaaccaggg aaaatgtgta gagggcatgg tggagatctt cgacatgctg ctggctacat
    12241 catctcggtt ccgcatgatg aatctgcagg gagaggagtt tgtgtgcctc aaatctatta
    12301 ttttgcttaa ttctggagtg tacacatttc tgtccagcac cctgaagtct ctggaagaga
    12361 aggaccatat ccaccgagtc ctggacaaga tcacagacac tttgatccac ctgatggcca
    12421 aggcaggcct gaccctgcag cagcagcacc agcggctggc ccagctcctc ctcatcctct
    12481 cccacatcag gcacatgagt aacaaaggca tggagcatct gtacagcatg aagtgcaaga
    12541 acgtggtgcc cctctatgac ctgctgctgg agatgctgga cgcccaccgc ctacatgcgc
    12601 ccactagccg tggaggggca tccgtggagg agacggacca aagccacttg gccactgcgg
    12661 gctctacttc atcgcattcc ttgcaaaagt attacatcac gggggaggca gagggtttcc
    12721 ctgccacagt ctgagtttaa acaaacctag aggatctttg tgaaggaacc ttacttctgt
    12781 ggtgtgacat aattggacaa actacctaca gagatttaaa gctctaaggt aaatataaaa
    12841 tttttaagtg tataatgtgt taaactactg attctaattg tttgtgtatt ttagattcca
    12901 acctatggaa ctgatgaatg ggagcagtgg tggaatgcct ttaatgagga aaacctgttt
    12961 tgctcagaag aaatgccatc tagtgatgat gaggctactg ctgactctca acattctact
    13021 cctccaaaaa agaagagaaa ggtagaagac cccaaggact ttccttcaga attgctaagt
    13081 tttttgagtc atgctgtgtt tagtaataga actcttgctt gctttgctat ttacaccaca
    13141 aaggaaaaag ctgcactgct atacaagaaa attatggaaa aatatttgat gtatagtgcc
    13201 ttgactagag atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa
    13261 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
    13321 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
    13381 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
    13441 tgtctggatc cgcatgcctg caggtcggag tactgtcctc cgagcggagt actgtcctcc
    13501 gagcggagta ctgtcctccg agcggagtac tgtcctccga gcggagtact gtcctccgag
    13561 cggagactct agcgagcgcc ggagtataaa tagaggcgct tcgtctacgg agcgacaatt
    13621 caattcaaac aagcaaagtg aacacgtcgc taagcgaaag ctaagcaaat aaacaagcgc
    13681 agctgaacaa gctaaacaat ctgcagtaaa gtgcaagtta aagtgaatca attaaaagta
    13741 accagcaacc aagtaaatca actgcaacta ctgaaatctg ccaagaagta attattgaat
    13801 acaagaagag aactctgaat agggaattgg gaattcgtta acagatctgc ggccgcggct
    13861 cgagggtacc tctagaggat ctttgtgaag gaaccttact tctgtggtgt gacataattg
    13921 gacaaactac ctacagagat ttaaagctct aaggtaaata taaaattttt aagtgtataa
    13981 tgtgttaaac tactgattct aattgtttgt gtattttaga ttccaaccta tggaactgat
    14041 gaatgggagc agtggtggaa tgcctttaat gaggaaaacc tgttttgctc agaagaaatg
    14101 ccatctagtg atgatgaggc tactgctgac tctcaacatt ctactcctcc aaaaaagaag
    14161 agaaaggtag aagaccccaa ggactttcct tcagaattgc taagtttttt gagtcatgct
    14221 gtgtttagta atagaactct tgcttgcttt gctatttaca ccacaaagga aaaagctgca
    14281 ctgctataca agaaaattat ggaaaaatat ttgatgtata gtgccttgac tagagatcat
    14341 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
    14401 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
    14461 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
    14521 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatccacta
    14581 gtgtcgacga tgtaggtcac ggtctcgaag ccgcggtgcg ggtgccaggg cgtgcccttg
    14641 ggctccccgg gcgcgtactc cacctcaccc atctggtcca tcatgatgaa cgggtcgagg
    14701 tggcggtagt tgatcccggc gaacgcgcgg cgcaccggga agccctcgcc ctcgaaaccg
    14761 ctgggcgcgg tggtcacggt gagcacggga cgtgcgacgg cgtcggcggg tgcggatacg
    14821 cggggcagcg tcagcgggtt ctcgacggtc acggcgggca tgtcgacact agttctagcc
    14881 agcttttgtt ccctttagtg agggttaatt tcgagcttgg cgtaatcatg gtcatagctg
    14941 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
    15001 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
    15061 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
    15121 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
    15181 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
    15241 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
    15301 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
    15361 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
    15421 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
    15481 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
    15541 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
    15601 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
    15661 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
    15721 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
    15781 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
    15841 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
    15901 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
    15961 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
    16021 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
    16081 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
    16141 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
    16201 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
    16261 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
    16321 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
    16381 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
    16441 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
    16501 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
    16561 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
    16621 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
    16681 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
    16741 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
    16801 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
    16861 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
    16921 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
    16981 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
    17041 caaatagggg ttccgcgcac atttccccga aaagtgccac
//
