LOCUS       sequence-441808-        9023 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  dop-2 fluorescent neural reporter driving nuclear mNeptune2.5 
            expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9023)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 9023)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY76
FEATURES             Location/Qualifiers
     source          1..9023
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4698..4718
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4743..4763
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4785..4805
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(6614..6636)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6736..6755
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6833..6851)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6919..7023
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7024..7092,7093..7884)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7242..7261)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8055..8643
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8544..8563
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    8931..8952
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8967..8972,8973..8990,8991..8997)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9005..9021
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(9010..9023,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcttctaca cagggttctg
      181 gctcgacccc gcgcttcatt ggacactgaa atggcgggaa aaaactttga aatcgcaaga
      241 ggaattttca cgcagcgcgt tgaaaaaagt gtatgcattt gcgcgtgacg gtgtttcttc
      301 aagttttgat actcctagaa tattctgaag tttcaataat tttaaaaaaa tatcaaaaat
      361 tgttataatt ttcagaaacg ctcgccaaaa actttcggaa aggaccggaa aatgtttcat
      421 atgatttgaa gaacgtagag aaaattatac gttcaagaaa tttagaaata gttccaggaa
      481 acttgaggtt ttaagttgta attgctcaga acttagttat ataatattta tgttgctcga
      541 aaatttccgg taaccaggga aaaaacgttc catctgcaaa gaaggcttag aaaatttaga
      601 atttgatttc aattttgagt acgccagtcg gagcacgcgc ttcagcgcgt gcgaacggct
      661 ggtaaattat attgaatatt gtggaatgga acagaaaagt gggaacgggt tgaggccttg
      721 ttacataacc ttaccattta ttaatgtttc caaaaaatag ggataaagcc tgtgtctgat
      781 gaacgaaagt taagaatgat tgctttttgc aaggtttact atagcactga gaacacgatg
      841 agtgaaaaaa tgagttcgaa gtttgctttt aggagttttc catctgtaag cgtagatgtt
      901 cctagttctt tcagaaggta atatttattc aaaaattaaa aaaaaaatca gagcacattt
      961 taaaatttcg tttagacgaa ccccattttt tgcggaccta aacatgatat tcaggtcaac
     1021 aaaaaatgat atttacaaat tttcagaact tacacaccag tcgaaaattg cattgagaat
     1081 ttcaaatcaa cccaggtttt cttgttttga acagctcttc gctgttcaaa aattcattct
     1141 aaaattgtcc gattttttga actatccttg ttcctctttc tctctttttt cggtcaattc
     1201 tattttaatc cagagcactt ttaaatcgaa taaacattta gttgggatgc caattaacaa
     1261 acatttcgtc cccccgaact tgatctaatt acaactttca ctcctttcaa tgttctgttt
     1321 gaaactcttt gaatctttca gaatttttcc cagaaaaact tcttcttttg ttctcacgtt
     1381 attttatcat tttacattca agttctgaaa gatcaaatca gactatgaat aaggcaaacc
     1441 tttttacgag aaaattgaat tgcgttggtg ttcaatgttt ttatgccgta gattagtagt
     1501 tatctgaatc agttttgtct atacattttc tttttcaact tgcgttgatt tccacgtaca
     1561 acttattcat tttcatgaag aacaatttat ttgttatctt caattgtcca tcttttgcct
     1621 ttgctccttc caatttcttg atttttttgt ttgtacgtca cttcacatag cttctccttt
     1681 ttctttttct tctaccaaat tgataattga tgagtggcgg agcacccagt ggagtagtgt
     1741 gagatttaca tgtatgtgaa tgggggacta gatttgtttt gtgttgtgga tgtgtattcc
     1801 gtttctttat ttccattcat tctatcatca acacatgtat gttcttccgg gaaacttttt
     1861 ttcgcactgg ttccaaagtg cccgtaattt gcaagtttaa gctaaaaatc aattcattgt
     1921 tgggcttgtc taggaatttt taatatattc cagtattttc attatttatc tactagagat
     1981 taaagctaat aatgagtcaa atagtaaaag accgatggaa aaatcagttc ctgaaggtat
     2041 ttttggtaat gctgaatcat ccatcaaaac ctttttcaag gattcgtaaa attactataa
     2101 agtttgatat taacaacagt taaaattttg caatatttgt actgacagta agttcactaa
     2161 tttcaataaa aactttgttt aaatttttct tttttatttt gccgttgttt ttgttgttaa
     2221 aattgaccaa aattgatatt tgtatgttat tattcaaaaa ctaaaaaaga atctcgccaa
     2281 tacactttta tcataaaaca tcttttaaaa caaaacaatt aaactacgag tagccacagt
     2341 attctcaaac actgtgatct cctgatattg ttctatatgg tgataattta ttattttatt
     2401 tacactaaaa cgtgtgtggc gttatatttt cccataacta aactttatca gaatttcagg
     2461 agatcaaaca aagaagtgac aattagacca catgtggtaa actgttaatg ccattttttt
     2521 actcgtgatt aataattatc agtgacaatt caattaaagt attcaccgaa tgagcacgtt
     2581 atacgaattc tgtaaaatat gtttaaaaac ttattccaaa taatagtatt ctgcgaaatt
     2641 atttttgtga ttttataagt caccgatata taaatttcat tacgctgctt ttgaggtatg
     2701 actttagatt tgcgtggtag taacttataa atgcttgcca taatcatgat gatgacaatt
     2761 gttataggtt gtcgaaattt atttagaaga ataaaatact tgtcaactgg aaagaataca
     2821 tttcattttg aaacatacag ttttgtatat aatttcgtag attgaataag cttcctgacc
     2881 atagacaaaa atctccgata caactttgtg gttactaatt tagagttaaa ttagtttagc
     2941 tccctattag tattctctag ttacttccaa ctatacagct tcgttgccta attgtcaaaa
     3001 tttttatgag aactatctaa gcattgacgt aggtttcatt aacttgttaa ctcacggttg
     3061 tatgaaacaa atttcagttt ttaaagtgtt ttcgatgttt ctaacatttc tttcaagaaa
     3121 ccccatattt ttgtttgatc cgtgcactag attaaaaaaa aaacctttac actttggctt
     3181 ttttctgttt tcatataaat ttgatcttgc ttttttacac tagaggtccg aaaaagtaac
     3241 acccaattta gaaaaatagc caaaacagga ggaacggctc gcaatttatg tgcatttttt
     3301 tgtgtcaact ccaccccaag gcttcttctt ctgctacttc tacatctcta cgcctgtcct
     3361 ccgcccatct caaagttcgt ttatgtcttg gggaccgcat tttctccgcc cctttactct
     3421 tcgttttttc tttatttagt aaagaagaga tgtggaaaag atattcatac tttccttccg
     3481 tctaaaattc aactggcttc tgaaggtttc tgctcagtct agggccactt ttaatagttc
     3541 acaaataaac tattgttcag tcttgacaga agaaaaacat tcaatgacct taactttatt
     3601 ccttattttt gtcattagac atctcgcact ttgctcttta cttccttgcc tattttcaaa
     3661 agacaactca tcgttggacc cttacccccc gagattcaaa aattgaatat tcaattgatg
     3721 tgaaagaaat catctgggct accagagacg tgcctcctgg atttcttctc tttcttgata
     3781 gatttggaga catgaacccc atcattgcca gacctgcact tttaattttt ttttcttatt
     3841 ggtgattttt gggctgcaat aacctcaaaa actatttttt tttaaatgtt tgaaggtctt
     3901 ccaaaatatt catgacagaa atacaattgt tcgtgttggt taatgtgctg aaagtctatg
     3961 tacaactttt gtaatttcca caatgacaac tttttttcgt agctaaattt cacattttgt
     4021 gtgacttccc aacttaattt tattatttgt tgatcactga tttaggatta agagtaagct
     4081 taatgtgtgc tttgtgttag aaattttaaa tatttttaaa gattttactt taagagagaa
     4141 taatgatgtt tttcaatgtt tagtattgat gtatcaaata cattacgttg acagtcatct
     4201 gagcattggt aaagaatttc aaactttgag cgaaacactt agaactttcc aatcaaatta
     4261 tatttttcta actcttcaga ttctttctgt tatcgagtct aaaaaacttc aaaattcaat
     4321 tccatttttg aagtgttccg tgcctttttt agttttaaaa cattctgaga aactaaaatc
     4381 tattacgttt cagaaagtag gaccatttgt agaactactt acagcaaaaa aacacttgaa
     4441 aatttttgtt ctttacattt tagcattaat ccttggaaca cctttgtttt taccttcttc
     4501 aaaccgtctt gttggcttaa aaaatgtaat aaaaaactgt tttaaggtga tccaactccc
     4561 ccgggattgg ccaaaggacc caaaggtatg tttcgaatga tactaacata acatagaaca
     4621 ttttcaggag gacccttgga gggtaccggt tttcccagtc acgacgcgtg gtaccgagct
     4681 cagaaaaaat gaccgctcca aagaagaaac gcaaagtacc gagctcagaa aaaatgacgg
     4741 cacctaaaaa gaaacgcaaa gtaccggtcg ccgagaagac agccccaaaa aagaagcgta
     4801 aagttccacg cgtcatggga gtaagtttaa acagttcggt actaactaac catacatatt
     4861 taaattttca gcgcgctgtc tccaagggag aggagctcat caaggagaac atgcacacca
     4921 agctctacat ggagggaacc gtcaacaacc accacttcaa gtgcacccac gaaggggagg
     4981 gcaagcccta cgagggcacc cagaccaaca ggattaaggt ggtggaggga ggccccctgc
     5041 cgttcgcatt cgacatcctg gccacctgct ttatgtacgg gagcaagacc ttcatcaacc
     5101 acacccaagg aatcccagac ttcttcaagc aatccttcgt aagtttaaac atgattttac
     5161 taactaacta atctgattta aattttcagc ctgagggctt cacatgggag agagtcacca
     5221 catacgaaga cgggggcgtg cttaccgtta cccaggacac cagcctccag gacggctgct
     5281 tgatctacaa cgtcaagctc agaggggtga acttcccatc caacggccct gtgatgcaga
     5341 agaaaacact cggctgggag gccagtaccg agaccctgta ccccgctgac ggcggcctgg
     5401 aaggcagatg cgacatggcc ctgaagctcg tgggcggggg ccacctgcac tgcaacctga
     5461 agaccacata cagatccaag aaacccgcta agaacctcaa gatgccagga gtaagtttaa
     5521 acatatatat actaactaac cctgattatt taaattttca ggtctacttt gtggaccgca
     5581 gactggaaag aatcaaggag gccgacaatg agacctacgt cgagcagcac gaggtggctg
     5641 tggccagata ctgcgacctc cctagcaaac tggggcacaa acttaatggc atggacgagc
     5701 tgtacaagta agcgccggtc gctaccatta ccaacttgtc tggtgtcaaa aataataggg
     5761 gccgctgtca tcagagtaag tttaaactga gttctactaa ctaacgagta atatttaaat
     5821 tttcagcatc tcgcgcccgt gcctctgact tctaagtcca attactcttc aacatcccta
     5881 catgctcttt ctccctgtgc tcccaccccc tatttttgtt attatcaaaa aacttctctt
     5941 aatttctttg ttttttagct tcttttaagt cacctctaac aatgaaattg tgtagattca
     6001 aaaatagaat taattcgtaa taaaaagtcg aaaaaaattg tgctccctcc ccccattaat
     6061 aataattcta tcccaaaatc tacacaatgt tctgtgtaca cttcttatgt tttttacttc
     6121 tgataaattt ttttgaaaca tcatagaaaa aaccgcacac aaaatacctt atcatatgtt
     6181 acgtttcagt ttatgaccgc aatttttatt tcttcgcacg tctgggcctc tcatgacgtc
     6241 aaatcatgct catcgtgaaa aagttttgga gtatttttgg aatttttcaa tcaagtgaaa
     6301 gtttatgaaa ttaattttcc tgcttttgct ttttggggtt tcccctattg tttgtcaaga
     6361 tttcgaggac ggcgtttttc ttgctaaaat cacaagtatt gatgagcacg atgcaagaaa
     6421 gatcggaaga aggtttgggt ttgaggctca gtggaaggtg agtagaagtt gataatttga
     6481 aagtggagta gtgtctatgg ggtttttgcc ttaaatgaca gaatacattc ccaatatacc
     6541 aaacataact gtttcctact agtcggccgt acgggccctt tcgtctcgcg cgtttcggtg
     6601 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
     6661 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
     6721 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
     6781 aaataccgca cagatgcgta aggagaaaat accgcatcag gcggccttaa gggcctcgtg
     6841 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     6901 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     6961 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     7021 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     7081 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     7141 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     7201 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     7261 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     7321 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     7381 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     7441 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     7501 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     7561 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     7621 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     7681 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     7741 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     7801 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     7861 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     7921 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     7981 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     8041 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     8101 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     8161 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     8221 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     8281 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     8341 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     8401 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     8461 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     8521 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     8581 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     8641 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     8701 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     8761 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgatgaagcg
     8821 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     8881 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     8941 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     9001 ggaattgtga gcggataaca att
//
