LOCUS       sequence-441809-        8624 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  str-2 fluorescent neural reporter driving nuclear mTagBFP2 
            expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8624)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 8624)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY134
FEATURES             Location/Qualifiers
     source          1..8624
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3989..4009
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4031..4051
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(6215..6237)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6337..6356
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6434..6452)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6520..6624
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6625..6693,6694..7485)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6843..6862)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7656..8244
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8145..8164
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8398..8415
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8532..8553
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8568..8573,8574..8591,8592..8598)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8606..8622
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(8611..8624,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagc gtcaagagac
      181 aggatacaac tgataataca aaatgaatgt aagagtttct ccactgaatt cccatttcat
      241 tacactttta attagtaagc actcacccct ggccagcctc ctgaataata tagataaact
      301 gaaccaaatg ggaacgggtg tcccatactc ctacagtaac caacggcggg aatcataaaa
      361 gcggtacatc cgaatggtat aagaaacaac aaaagtaata aaatactcat taattttggg
      421 cagatctgaa aaaatagtac atgtatacat atattcccat gtgaactaat acttccttac
      481 aaaaaagtga ttattgggac gtaacaagat tatcactctg aacagtgaca agtagaatgg
      541 aagaaaaaag acagaatagt taaaatatta aaaattaaga aaatagtttt tataaaatga
      601 tttggttcga caaacgcaca ccatgaagtg attattccag ttgcaggaat agagccacga
      661 agaaactcaa acatgaaaaa cgaaatactc agtaaattga taggaaacgt ttctaaatct
      721 ttcgcttctt gagtttaaat atttcgttgc ataaatcaac tcacacagaa aaatagcatc
      781 gcaatgcaga tgaaaatata tttattcatt ggagcaattt tggaccatcc aataagtgct
      841 gacaattttc cacattacag caatggtagg tggcatataa atcaatggga tcaacgccag
      901 aattgtagga aacgtaaaag tatactgaaa actttggtat tcggggattc cgtaaataga
      961 accgttcatt ttttttagat taccaattta cataaaatat gaatatcaat gatttgagaa
     1021 agaaaaacat gacgtagatg tgggcttgaa gtaattgtat tatatattta ttatatttta
     1081 ttacattatt attttgtgga ccggaagtaa aaattccttc atgaaaacca tttatgagat
     1141 tgaaaaataa cgtttccaaa aaacaaggct aaaaatattt aattgatgag aaaaatctat
     1201 atgaaaaaat aaaaaaatga aaaatttata aagtatcaaa aaaaatttag attaacattt
     1261 acgtaattat ttttagcctt ggattctcat cacattattt aatttaattt aatttaattt
     1321 gtttacgctc gcgaggagac gtggaaaaag ttgaaaaaaa aagaaaaatg ttcacataca
     1381 atagatcata aatacaatac actgaataag tgggaagaaa actaagaaaa ataaatagga
     1441 acgaaagttt gttaacagtt tgctccttta ccttgcattg cttatctcac tctccaccgc
     1501 ttgattttgc tccgaattcc accacatgcg cgcttctgaa cggtctgtgg gctctgacac
     1561 ccacctggtc gagttgaccg ttcaacatgg tgcatcgacc gggaatattg ctcgtgcaat
     1621 cgccaaacta tgtcttcgga tcgtaaattc gttcgtaaaa atcgatcgta aaaaactctc
     1681 catctccaat tccaggagct gacggttcta atgaaaccga aagttcaggg aaacgtgaga
     1741 ctctacggac tcgcaagagt caaaagtcta caaggggaac tcgtgcagcc acaccaggcc
     1801 gcttgaaaag cttcgctgcc ccaattaaag ggtacattac ccacgcaagg ttggtcaggc
     1861 aggaggaaat aatgttatgt gggtagttta tgttgcaatc atcagttgtc agacatctcg
     1921 ttattcaaga tctgatatat gtttaagaaa cttcgggtta ctcagcgtac tacaataaaa
     1981 tgctttctag tttcagtttt acatctgctc catgagactt tctttttgga acaagttttg
     2041 gaacgagtca tattattttt tcagaatgaa gcgttcttcg ccattgactg ctccgtaata
     2101 gatttgaagt cgtgttttac ctatataggc atgaggcagg cggagttgaa acaattgatt
     2161 atacactcat acagttttat cttggttcaa atttttgccc aatataaaat gtgcagctta
     2221 ttgatcatat taagacaaaa aagtgtttaa aaaaagtaac tccaattagg tattatgtat
     2281 gcaagttttc aaagaagcca accaaacagt gatgtgcgtt atttgaatta aatcttatct
     2341 tcccagatca gttttctcga attcatagtt tttaattttt ataaattgat atttcagcat
     2401 ttcgtaattc tcatgttcta aataggaaac atctgggatg cgttgtagac atgttgggac
     2461 aacgtcgaat atttttctca aattccattt ctatttcgtt ttaggcaaag tgaaacaaaa
     2521 aattttcaat accaaatatc agtctaagta attcgaaaaa cggtatttta tactcccaaa
     2581 aggctacgac gcctataaaa caaaactgac cagacttcca cacctgggag agatagggtc
     2641 agtttttgtt cgtttgtctc attggatttc ctcgctgata catgatttaa aacatacttt
     2701 tattgtatag aacgataatt tttgtgtggt ttattatgaa gcaaacatca ttcatacatc
     2761 aaatgcgtgt tttcagttca gcttcctttg ttcaccaata aagtttttaa ttccttgaaa
     2821 gggatggatt atccttttat cgaaaaacgt agaacttcgt gtacgtttta aaataatctt
     2881 tcaattcaag ctgtttcttt tattaatatt acattttttt agttgatttc acttaaaaaa
     2941 ctctattcat tcggattatt ggttgaagga actctgattt ggttgaaata tcaaatttaa
     3001 acataatact gttaatggtt cgattatacc aagaaaatag ttcaaatctt ctctcaataa
     3061 taatcgtaga cattgttttc aatagtatat agtctgtctc taattttcaa gctctttaga
     3121 gaattaaaca tattgttttt gtatttcagt tttattaatc cctgtttgtt ctcaacaatt
     3181 tcttatctag atcagttatt cagttatgta gatatgcttg agattctgaa atcggaaagt
     3241 ataatcataa acgaaatttt ggatattatt tgagctttta aatttttttt tcaacattag
     3301 ggtaacattt ttagtcgatc tttgtccatt ttctgagaac ctactatgtt ttttgtttga
     3361 gcttaaaaat tcgattgaac aactgtacag tatagcgtca cttcttagga ttcctgatat
     3421 tttatgctga actactggtt cataaaattt tctttcaaat aaaactgtct gatttgttta
     3481 ttacacaacg aagtttgtat gcgtaaacga taaaaacatc ttctagacag accaaagtct
     3541 ttcttcaata gtcaaatcat ttcaaattgt tcctaatgat cctttcatga atatttgtac
     3601 aaaataacag ttgacaaacc aaatattttg tgaaaaattg gagtgattag cgaatcccgt
     3661 aggaaaaacc aggagtcagt taagtgaagc attttcttgc aagaaagaaa taaatgattt
     3721 catgttccca actggtgata atatctctct aaggagttat catgtgttgt ttttggaaat
     3781 taatattctt cactttttgt gtttttattt caaccattct cgattataat tttgcttcca
     3841 gacttttttg tccgaatact cgtgatccat aaaacccggg attggccaaa ggacccaaag
     3901 gtatgtttcg aatgatacta acataacata gaacattttc aggaggaccc ttgcttggag
     3961 ggtaccgagc tcagaaaaaa tgaccgctcc aaagaagaag cgcaaggttc cagtcgagaa
     4021 gatgactgcc ccaaaaaaga aacgtaaggt cccgcggatg tcagagctta ttaaggagaa
     4081 tatgcatatg aaactgtaca tggaaggaac cgtggacaac catcacttta agtgcacttc
     4141 ggagggggaa ggcaaaccgt acgagggcac ccagaccatg cgaatcaagg tggtcgaggg
     4201 aggcccactg cccttcgcct tcgacattct ggtaagttta aacatatata tactaactaa
     4261 ccctgattat ttaaattttc aggctactag cttcctgtac ggcagcaaga cttttattaa
     4321 tcacacccag ggcatcccgg acttcttcaa acagtcgttc cctgagggct tcacatggga
     4381 gcgtgtgacc acatacgaag acggtggcgt tctcaccgct acccaggaca ccagcctcca
     4441 ggacggttgc ctcatatata atgtggtaag tttaaacagt tcggtactaa ctaaccatac
     4501 atatttaaat tttcagaaga tccggggagt aaacttcaca tccaatggtc cggttatgca
     4561 gaagaaaaca ctcggctggg aggccttcac cgagacgctg tatccggctg acggaggcct
     4621 ggaaggtcga aacgacatgg ccctgaagct cgtcggcggt agccatctca tcgcaaacgc
     4681 taagaccaca tatagatcca agaaaccggt aagtttaaac atgattttac taactaacta
     4741 atctgattta aattttcagg ctaagaacct taaaatgccg ggagtctact atgttgacta
     4801 cagactggaa cggatcaagg aggccaacaa cgagacctac gtcgagcagc acgaggttgc
     4861 agttgccaga tactgcgacc tcccgagcaa actgggtcac aagcttaatc ctaggatacc
     4921 ggtgggtgaa gacccaccaa agccatctgc caagggagcc aagaaggccg ccaagaccgt
     4981 tacgaagcca aaggacggaa agaagagacg tcatgcccgt aaggaatcat actccgtcta
     5041 catctaccgt gtcctcaagc aagttcatcc agacactgga gtttcctcca aagccatgtc
     5101 tatcatgaac tcttttgtca acgatgtctt cgagcgtatt gctgctgaag catcccgtct
     5161 tgctcactac aacaagcgtt ccacaatctc atcccgcgaa attcagaccg ctgtccgtct
     5221 gatccttcca ggagagcttg ccaagcacgc cgtgtctgag ggaaccaagg ccgttaccaa
     5281 gtacacttcc agcaagtagg aattccaact gagcgccggt cgctaccatt accaacttgt
     5341 ctggtgtcaa aaataatagg ggccgctgtc atcagagtaa gtttaaactg agttctacta
     5401 actaacgagt aatatttaaa ttttcagcat ctcgcgcccg tgcctctgac ttctaagtcc
     5461 aattactctt caacatccct acatgctctt tctccctgtg ctcccacccc ctatttttgt
     5521 tattatcaaa aaacttctct taatttcttt gttttttagc ttcttttaag tcacctctaa
     5581 caatgaaatt gtgtagattc aaaaatagaa ttaattcgta ataaaaagtc gaaaaaaatt
     5641 gtgctccctc cccccattaa taataattct atcccaaaat ctacacaatg ttctgtgtac
     5701 acttcttatg ttttttactt ctgataaatt tttttgaaac atcatagaaa aaaccgcaca
     5761 caaaatacct tatcatatgt tacgtttcag tttatgaccg caatttttat ttcttcgcac
     5821 gtctgggcct ctcatgacgt caaatcatgc tcatcgtgaa aaagttttgg agtatttttg
     5881 gaatttttca atcaagtgaa agtttatgaa attaattttc ctgcttttgc tttttggggt
     5941 ttcccctatt gtttgtcaag atttcgagga cggcgttttt cttgctaaaa tcacaagtat
     6001 tgatgagcac gatgcaagaa agatcggaag aaggtttggg tttgaggctc agtggaaggt
     6061 gagtagaagt tgataatttg aaagtggagt agtgtctatg gggtttttgc cttaaatgac
     6121 agaatacatt cccaatatac caaacataac tgtttcctac tagtcggccg tacgggccct
     6181 ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     6241 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     6301 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga
     6361 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
     6421 ggcggcctta agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     6481 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     6541 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     6601 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     6661 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     6721 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     6781 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     6841 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     6901 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     6961 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     7021 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     7081 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     7141 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     7201 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     7261 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     7321 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     7381 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     7441 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     7501 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     7561 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     7621 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     7681 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     7741 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     7801 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     7861 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     7921 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     7981 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     8041 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     8101 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     8161 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     8221 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     8281 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     8341 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     8401 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     8461 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     8521 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     8581 cttccggctc gtatgttgtg tggaattgtg agcggataac aatt
//
