LOCUS       sequence-441810-        5377 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  cho-1(3.1-2.6k) fluorescent neural reporter driving nuclear CyOFP1 
            expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5377)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 5377)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY74
FEATURES             Location/Qualifiers
     source          1..5377
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             742..762
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             784..804
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(2968..2990)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3090..3109
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3187..3205)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3273..3377
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3378..3446,3447..4238)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3596..3615)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4409..4997
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4898..4917
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5151..5168
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5285..5306
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5321..5326,5327..5344,5345..5351)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5359..5375
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(5364..5377,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagt tcaccaccct
      181 tcacgaccta taaatttatt ttcgatagtt gcaaaaaaag cgaagaaaac aagaaacgct
      241 tgacaaaaca tttccgcagt tgcaaatcct ttcttaaaac ttgtttgatt ggctcaatcc
      301 gttgctcttt tgctcatcct cctctctttc ccatacacac acatcgaaat atgaatcttc
      361 tcttaaaaag aaggttgtcc aattagtttc ccctattcat ttttccatgt tttcttataa
      421 caactttttc ttttgaatac tcctccagaa atgataaatc tttgtgagat gccaattaaa
      481 atttattttt gaattttatt tgttgaagga ttttatttgt tgaatgacct aaatagaggc
      541 tccgcccata tcttggcttt ctgcaatttg cataaaaggt tttccagttt tcgtaggtat
      601 tttttgaagg tcttccacgt aggcgttccc gggattggcc aaaggaccca aaggtatgtt
      661 tcgaatgata ctaacataac atagaacatt ttcaggagga cccttgcttg gagggtaccg
      721 agctcagaaa aaatgaccgc tccaaagaag aagcgcaagg ttccagtcga gaagatgact
      781 gccccaaaaa agaaacgtaa ggtcccgcgg atgtcagagc ttattaagga gaatatgcat
      841 atgaaactgt acatggaagg aaccgtggac aaccatcact ttaagtgcac ttcggagggg
      901 gaaggcaaac cgtacgaggg cacccagacc atgcgaatca aggtggtcga gggaggccca
      961 ctgcccttcg ccttcgacat tctggtaagt ttaaacatat atatactaac taaccctgat
     1021 tatttaaatt ttcaggctac tagcttcctg tacggcagca agacttttat taatcacacc
     1081 cagggcatcc cggacttctt caaacagtcg ttccctgagg gcttcacatg ggagcgtgtg
     1141 accacatacg aagacggtgg cgttctcacc gctacccagg acaccagcct ccaggacggt
     1201 tgcctcatat ataatgtggt aagtttaaac agttcggtac taactaacca tacatattta
     1261 aattttcaga agatccgggg agtaaacttc acatccaatg gtccggttat gcagaagaaa
     1321 acactcggct gggaggcctt caccgagacg ctgtatccgg ctgacggagg cctggaaggt
     1381 cgaaacgaca tggccctgaa gctcgtcggc ggtagccatc tcatcgcaaa cgctaagacc
     1441 acatatagat ccaagaaacc ggtaagttta aacatgattt tactaactaa ctaatctgat
     1501 ttaaattttc aggctaagaa ccttaaaatg ccgggagtct actatgttga ctacagactg
     1561 gaacggatca aggaggccaa caacgagacc tacgtcgagc agcacgaggt tgcagttgcc
     1621 agatactgcg acctcccgag caaactgggt cacaagctta atcctaggat accggtgggt
     1681 gaagacccac caaagccatc tgccaaggga gccaagaagg ccgccaagac cgttacgaag
     1741 ccaaaggacg gaaagaagag acgtcatgcc cgtaaggaat catactccgt ctacatctac
     1801 cgtgtcctca agcaagttca tccagacact ggagtttcct ccaaagccat gtctatcatg
     1861 aactcttttg tcaacgatgt cttcgagcgt attgctgctg aagcatcccg tcttgctcac
     1921 tacaacaagc gttccacaat ctcatcccgc gaaattcaga ccgctgtccg tctgatcctt
     1981 ccaggagagc ttgccaagca cgccgtgtct gagggaacca aggccgttac caagtacact
     2041 tccagcaagt aggaattcca actgagcgcc ggtcgctacc attaccaact tgtctggtgt
     2101 caaaaataat aggggccgct gtcatcagag taagtttaaa ctgagttcta ctaactaacg
     2161 agtaatattt aaattttcag catctcgcgc ccgtgcctct gacttctaag tccaattact
     2221 cttcaacatc cctacatgct ctttctccct gtgctcccac cccctatttt tgttattatc
     2281 aaaaaacttc tcttaatttc tttgtttttt agcttctttt aagtcacctc taacaatgaa
     2341 attgtgtaga ttcaaaaata gaattaattc gtaataaaaa gtcgaaaaaa attgtgctcc
     2401 ctccccccat taataataat tctatcccaa aatctacaca atgttctgtg tacacttctt
     2461 atgtttttta cttctgataa atttttttga aacatcatag aaaaaaccgc acacaaaata
     2521 ccttatcata tgttacgttt cagtttatga ccgcaatttt tatttcttcg cacgtctggg
     2581 cctctcatga cgtcaaatca tgctcatcgt gaaaaagttt tggagtattt ttggaatttt
     2641 tcaatcaagt gaaagtttat gaaattaatt ttcctgcttt tgctttttgg ggtttcccct
     2701 attgtttgtc aagatttcga ggacggcgtt tttcttgcta aaatcacaag tattgatgag
     2761 cacgatgcaa gaaagatcgg aagaaggttt gggtttgagg ctcagtggaa ggtgagtaga
     2821 agttgataat ttgaaagtgg agtagtgtct atggggtttt tgccttaaat gacagaatac
     2881 attcccaata taccaaacat aactgtttcc tactagtcgg ccgtacgggc cctttcgtct
     2941 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
     3001 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
     3061 tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
     3121 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcggcc
     3181 ttaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     3241 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     3301 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     3361 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     3421 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     3481 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     3541 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     3601 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     3661 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     3721 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     3781 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     3841 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     3901 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     3961 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     4021 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     4081 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     4141 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     4201 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     4261 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     4321 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     4381 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     4441 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     4501 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     4561 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     4621 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     4681 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     4741 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     4801 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     4861 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     4921 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     4981 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     5041 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     5101 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     5161 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     5221 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     5281 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     5341 ctcgtatgtt gtgtggaatt gtgagcggat aacaatt
//
