LOCUS       sequence-441838-        8803 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral vector encoding biosensor chains to detect rapamycin 
            (FRB/FKBP domains) and respond with split NanoLuciferase 
            reconstitution (11S/114 fragment). WT Glycophorin A (GPA) scaffold..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8803)
  AUTHORS   Dolberg TB, Gunnels TF, Ling T, Sarnese KA, Crispino JD, Leonard JN
  TITLE     Building Synthetic Biosensors Using Red Blood Cell Proteins.
  JOURNAL   ACS Synth Biol. 2024 Apr 19;13(4):1273-1289. doi: 
            10.1021/acssynbio.3c00754. Epub 2024 Mar 27.
  PUBMED    38536408
REFERENCE   2  (bases 1 to 8803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00754"; journalName: "ACS Synth Biol"; date: 
            "2024-04-19- 19"; volume: "13"; issue: "4"; pages: "1273-1289"
COMMENT     Sequence Label: L2085-WT-GPA-NLuc-Biosensor-Retrovirus-TD135
FEATURES             Location/Qualifiers
     source          1..8803
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    579..920
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             987..1403
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1333..1355
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1371..1387
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             1494..1523
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2313..2366
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             2445..2474
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             join(2484..2486,2487..2807)
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKLE"
     misc_feature    2869..2921
                     /label=MCS
                     /note="multiple cloning site"
     promoter        3889..4399
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4318..4336
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     regulatory      4415..4424
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4421..4423,4424..4426,4427..5140)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4421..4423,4424..4426,4427..5137)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4466..4487)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4727..4746)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5074..5095
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             5196..5710
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    5872..5888
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5896..5902,5903..5920,5921..5926))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5941..5962
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6079..6096)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6250..6838)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6330..6349)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7009..7800,7801..7869))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7632..7651
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7870..7974)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8042..8060
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8098..8120)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8220..8239
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8433..8455
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8583..8602
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             join(8802..8803,1..515)
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
ORIGIN
        1 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
       61 ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag agagacagca
      121 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
      181 acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca tcagatgttt
      241 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc
      301 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc
      361 ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt attcccaata
      421 aagcctcttg ctgtttgcat ccgaatcgtg gactcgctga tccttgggag ggtctcctca
      481 gattgattga ctgcccacct cgggggtctt tcatttggag gttccaccga gatttggaga
      541 cccctgccca gggaccaccg acccccccgc cgggaggtaa gctggccagc ggtcgtttcg
      601 tgtctgtctc tgtctttgtg cgtgtttgtg ccggcatcta atgtttgcgc ctgcgtctgt
      661 actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac gagttctgaa
      721 cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt tgtggcccga
      781 cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt ctggtaggag
      841 acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tggaaccgaa
      901 gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc tgtctgactg
      961 tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta agtttgacct
     1021 taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga
     1081 gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag
     1141 acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc
     1201 cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc
     1261 cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg
     1321 ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag
     1381 ccctcactcc ttctctaggc gccggaatta gatctctcga ccttaagctt ggtaccatgt
     1441 atggaaaaat aatctttgta ttactattgt cagaaattgt gagcatatca gcagagcaga
     1501 aactcatctc agaagaggat ctgggcgcgc ctatgctcga gatgtggcat gaaggcctgg
     1561 aagaggcatc tcgtttgtac tttggggaaa ggaacgtgaa aggcatgttt gaggtgctgg
     1621 agcccttgca tgctatgatg gaacggggcc cccagactct gaaggaaaca tcctttaatc
     1681 aggcctatgg tcgagattta atggaggccc aagagtggtg caggaagtac atgaaatcag
     1741 ggaatgtcaa ggacctcctc caagcctggg acctctatta tcatgtgttc cgacgaatct
     1801 caaagactag aaccggttta agtaccactg aggtggcaat gcacacttca acctcttctt
     1861 cagtcacaaa gagttacatc tcatcacaga caaatgatac gcacaaacgg gacacatatg
     1921 cagccactcc tagagctcat gaagtttcag aaatttctgt tagaactgtt taccctccag
     1981 aagaggaaac cggagaaagg gtacaacttg cccatcattt ctctgaacca gagataacac
     2041 tcattatttt tggggtgatg gctggtgtta ttggaacgat cctcttaatt tcttacggta
     2101 ttcgccgact gataaagaaa agcccatctg atgtaaaacc tctcccctca cctgacacag
     2161 acgtgccttt aagttctgtt gaaatagaaa atccagagac aagtgatcaa ttaattaagg
     2221 gggggtctgg aggcagtggt gggtctggtg gtggttctgg ggggagtggg ggttcaggcg
     2281 tgaccggcta ccggctgttc gaggagattc tggagggcag aggaagtctg ctaacatgcg
     2341 gtgacgtcga ggagaatcct ggcccagata tccttaagct tggtaccatg tatggaaaaa
     2401 taatctttgt attactattg tcagaaattg tgagcatatc agcagagcag aaactcatct
     2461 cagaagagga tctgggcgcg cctatgggag tgcaggtgga aaccatctcc ccaggagacg
     2521 ggcgcacctt ccccaagcgc ggccagacct gcgtggtgca ctacaccggg atgcttgaag
     2581 atggaaagaa atttgattcc tcccgggaca gaaacaagcc ctttaagttt atgctaggca
     2641 agcaggaggt gatccgaggc tgggaagaag gggttgccca gatgagtgtg ggtcagagag
     2701 ccaaactgac tatatctcca gattatgcct atggtgccac tgggcaccca ggcatcatcc
     2761 caccacatgc cactctcgtc ttcgatgtgg agcttctaaa actggaaagt gctggtggta
     2821 gtgctggtgg tagtgctggt ggtagtgctg gtggtagtgc tggtggtcct cgagctcaag
     2881 cttcgaattc tgcagtcgac ggtaccgcgg gcccgggatc caccggatct agaaccggtt
     2941 taagtaccac tgaggtggca atgcacactt caacctcttc ttcagtcaca aagagttaca
     3001 tctcatcaca gacaaatgat acgcacaaac gggacacata tgcagccact cctagagctc
     3061 atgaagtttc agaaatttct gttagaactg tttaccctcc agaagaggaa accggagaaa
     3121 gggtacaact tgcccatcat ttctctgaac cagagataac actcattatt tttggggtga
     3181 tggctggtgt tattggaacg atcctcttaa tttcttacgg tattcgccga ctgataaaga
     3241 aaagcccatc tgatgtaaaa cctctcccct cacctgacac agacgtgcct ttaagttctg
     3301 ttgaaataga aaatccagag acaagtgatc aattaattaa gggggggtct ggaggcagtg
     3361 gtgggtctgg tggtggttct ggggggagtg ggggttcagg cgtcttcaca ctcgaagatt
     3421 tcgttgggga ctgggaacag acagccgcct acaacctgga ccaagtcctt gaacagggag
     3481 gtgtgtccag tttgctgcag aatctcgccg tgtccgtaac tccgatccaa aggattgtcc
     3541 ggagcggtga aaatgccctg aagatcgaca tccatgtcat catcccgtat gaaggtctga
     3601 gcgccgacca aatggcccag atcgaagagg tgtttaaggt ggtgtaccct gtggatgatc
     3661 atcactttaa ggtgatcctg ccctatggca cactggtaat cgacggggtt acgccgaaca
     3721 tgctgaacta tttcggacgg ccgtatgaag gcatcgccgt gttcgacggc aaaaagatca
     3781 ctgtaacagg gaccctgtgg aacggcaaca aaattatcga cgagcgcctg atcacccccg
     3841 acggctccat gctgttccga gtaaccatca acagctaaca attcccacgg ggttggggtt
     3901 gcgccttttc caaggcagcc ctgggtttgc gcagggacgc ggctgctctg ggcgtggttc
     3961 cgggaaacgc agcggcgccg accctgggtc tcgcacattc ttcacgtccg ttcgcagcgt
     4021 cacccggatc ttcgccgcta cccttgtggg ccccccggcg acgcttcctg ctccgcccct
     4081 aagtcgggaa ggttccttgc ggttcgcggc gtgccggacg tgacaaacgg aagccgcacg
     4141 tctcactagt accctcgcag acggacagcg ccagggagca atggcagcgc gccgaccgcg
     4201 atgggctgtg gccaatagcg gctgctcagc agggcgcgcc gagagcagcg gccgggaagg
     4261 ggcggtgcgg gaggcggggt gtggggcggt agtgtgggcc ctgttcctgc ccgcgcggtg
     4321 ttccgcattc tgcaagcctc cggagcgcac gtcggcagtc ggctccctcg ttgaccgaat
     4381 caccgacctc tctccccagg gggatccacc ggtcgccacc atggtgagca agggcgagga
     4441 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     4501 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
     4561 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
     4621 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     4681 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     4741 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     4801 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     4861 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     4921 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     4981 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
     5041 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     5101 cgccgggatc actctcggca tggacgagct gtacaagtaa agcggccgca tcgataaaat
     5161 aaaagatttt atttagtctc cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg
     5221 gcaagctagc ttaagtaacg ccattttgca aggcatggaa aatacataac tgagaataga
     5281 gaagttcaga tcaaggttag gaacagagag acagcagaat atgggccaaa caggatatct
     5341 gtggtaagca gttcctgccc cggctcaggg ccaagaacag atggtcccca gatgcggtcc
     5401 cgccctcagc agtttctaga gaaccatcag atgtttccag ggtgccccaa ggacctgaaa
     5461 tgaccctgtg ccttatttga actaaccaat cagttcgctt ctcgcttctg ttcgcgcgct
     5521 tctgctcccc gagctcaata aaagagccca caacccctca ctcggcgcgc cagtcctccg
     5581 atagactgcg tcgcccgggt acccgtgtat ccaataaacc ctcttgcagt tgcatccgac
     5641 ttgtggtctc gctgttcctt gggagggtct cctctgagtg attgactacc cgtcagcggg
     5701 ggtctttcat gggtaacagt ttcttgaagt tggagaacaa cattctgagg gtaggagtcg
     5761 aatattaagt aatcctgact caattagcca ctgttttgaa tccacatact ccaatactcc
     5821 tgaaatagtt cattatggac agcgcagaaa gagctgggga gaattgtgaa attgttatcc
     5881 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     5941 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     6001 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     6061 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     6121 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     6181 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     6241 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     6301 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     6361 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     6421 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     6481 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     6541 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     6601 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     6661 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     6721 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     6781 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     6841 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     6901 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     6961 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     7021 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     7081 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     7141 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     7201 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     7261 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     7321 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     7381 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     7441 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     7501 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     7561 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     7621 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     7681 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     7741 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     7801 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     7861 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     7921 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     7981 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
     8041 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct
     8101 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
     8161 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc
     8221 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
     8281 cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga
     8341 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
     8401 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
     8461 gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc
     8521 accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca
     8581 tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc
     8641 acgatgcgtc cggcgtagag gcgattagtc caatttgtta aagacaggat atcagtggtc
     8701 caggctctag ttttgactca acaatatcac cagctgaagc ctatagagta cgagccatag
     8761 ataaaataaa agattttatt tagtctccag aaaaaggggg gaa
//
