LOCUS       sequence-441841-       10526 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human codon-optimized St1Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10526)
  AUTHORS   Seo SY, Min S, Lee S, Seo JH, Park J, Kim HK, Song M, Baek D, Cho 
            SR, Kim HH
  TITLE     Massively parallel evaluation and computational prediction of the 
            activities and specificities of 17 small Cas9s.
  JOURNAL   Nat Methods. 2023 Jul;20(7):999-1009. doi: 
            10.1038/s41592-023-01875-2. Epub 2023 May 15.
  PUBMED    37188955
REFERENCE   2  (bases 1 to 10526)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-023-01875-2"; journalName: "Nat Methods"; date: 
            "2023-07"; volume: "20"; issue: "7"; pages: "999-1009"
COMMENT     Sequence Label: pLenti6.3-St1Cas9-BlastR
FEATURES             Location/Qualifiers
     source          1..10526
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1079..1312
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1497..1541
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     enhancer        1820..2123
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2124..2327
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2278..2298
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2324..2348
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        2399..2417
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      2425..2434
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2437..2457
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5848..5868
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5875..5898
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5908..5964
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5965..6363
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    6388..6976
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6441..6461)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6859..6870)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7047..7280
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7352..7473
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7389..7408)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7443..7462
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7513..7648
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(7669..7687)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7697..7714)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7697..7713)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7706..7728)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7855..8310
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7942..7961)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8152..8173
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8336..8440
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8441..8509,8510..9301)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8659..8678)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9472..10060
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9961..9980
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10214..10231
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10348..10369
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10384..10389,10390..10407,10408..10414)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10422..10438
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10427..10449
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10446..10462
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10446..10462
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10481..10501
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10483..10501
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccggccgc tgatcttcag acctggagga ggagatatga
      961 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
     1021 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
     1081 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
     1141 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
     1201 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
     1261 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
     1321 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
     1381 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
     1441 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
     1501 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
     1561 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
     1621 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
     1681 cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag
     1741 aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc
     1801 gataagcttg ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     1861 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     1921 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     1981 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     2041 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     2101 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
     2161 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
     2221 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
     2281 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc
     2341 gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga caccgactta
     2401 atacgactca ctatagggag agccgccacc atggcaccga agaagaagcg taaagtcgga
     2461 atccacggag ttcctgcggc aagcgatctg gtgctgggcc tggacatcgg aatcggctcc
     2521 gtgggagtgg gcatcctgaa taaggtgaca ggcgagatca tccacaagaa ctcccggatc
     2581 ttcccagcag cacaggcaga gaacaatctg gtgcggagaa ccaatagaca gggcaggcgc
     2641 ctggcccgga gaaagaagca caggcgcgtg cggctgaaca gactgttcga ggagtctggc
     2701 ctgatcaccg actttacaaa gatcagcatc aacctgaatc cctaccagct gagggtgaag
     2761 ggcctgaccg atgagctgtc taatgaggag ctgttcatcg ccctgaagaa catggtgaag
     2821 caccgcggca tctcctacct ggacgatgcc tctgacgatg gcaatagctc cgtgggcgac
     2881 tatgcccaga tcgtgaagga gaacagcaag cagctggaga ccaagacacc cggccagatc
     2941 cagctggaga gataccagac ctatggccag ctgagaggcg atttcacagt ggagaaggac
     3001 ggcaagaagc acagactgat caacgtgttt ccaacctccg cctacaggtc tgaggccctg
     3061 cgcatcctgc agacccagca ggagttcaat ccccagatca cagatgagtt tatcaaccgg
     3121 tatctggaga tcctgacagg caagagaaag tactatcacg gcccaggcaa tgagaagtcc
     3181 cggaccgatt acggcaggta tcgcacctct ggcgagacac tggacaacat cttcggcatc
     3241 ctgatcggca agtgcacctt ctaccccgat gagtttagag ccgccaaggc ctcttataca
     3301 gcccaggagt ttaacctgct gaatgacctg aacaatctga ccgtgcctac cgagacaaag
     3361 aagctgagca aggagcagaa gaaccagatc atcaattacg tgaagaacga gaaggctatg
     3421 ggcccagcca agctgttcaa gtatatcgcc aagctgctgt cttgtgacgt ggccgacatc
     3481 aagggctacc gcatcgacaa gagcggcaag gccgagatcc acacatttga ggcctacagg
     3541 aagatgaaga ccctggagac actggacatc gagcagatgg atcgcgagac cctggacaag
     3601 ctggcctatg tgctgaccct gaatacagag agggagggca tccaggaggc cctggagcac
     3661 gagttcgccg atggcagctt ttcccagaag caggtggacg agctggtgca gttccgcaag
     3721 gccaattcta gcatcttcgg caagggctgg cacaactttt ccgtgaagct gatgatggag
     3781 ctgatccctg agctgtacga gacctctgag gagcagatga ccatcctgac acggctgggc
     3841 aagcagaaga ccacatcctc tagcaacaag accaagtaca tcgacgagaa gctgctgaca
     3901 gaggaaatct acaatccagt ggtggcaaag tccgtgcggc aggccatcaa gatcgtgaac
     3961 gccgccatca aggagtacgg cgacttcgat aatatcgtga tcgagatggc cagagagacc
     4021 aacgaggacg atgagaagaa ggccatccag aagatccaga aggccaataa ggacgagaag
     4081 gatgccgcca tgctgaaggc cgccaaccag tacaatggca aggccgagct gcctcacagc
     4141 gtgtttcacg gccacaagca gctggccaca aagatcaggc tgtggcacca gcagggagag
     4201 cggtgcctgt ataccggcaa gacaatctcc atccacgatc tgatcaacaa ttctaaccag
     4261 ttcgaggtgg accacatcct gcccctgagc atcacctttg acgattccct ggccaataag
     4321 gtgctggtgt acgccaccgc caaccaggag aagggccaga gaacacctta tcaggccctg
     4381 gactctatgg acgatgcctg gagcttcagg gagctgaagg cctttgtgcg cgagagcaag
     4441 accctgtcca acaagaagaa ggagtacctg ctgacagagg aggacatcag caagttcgac
     4501 gtgcggaaga agtttatcga gagaaatctg gtggacacca gatatgcctc ccgcgtggtg
     4561 ctgaacgcac tgcaggagca cttccgggcc cacaagatcg ataccaaggt gagcgtggtg
     4621 agaggccagt ttacatccca gctgcggaga cactggggca tcgagaagac cagggataca
     4681 taccaccacc acgcagtgga cgcactgatc atcgcagcct cctctcagct gaatctgtgg
     4741 aagaagcaga agaacacact ggtgtcttat agcgaggacc agctgctgga catcgagacc
     4801 ggcgagctga tcagcgacga tgagtacaag gagtccgtgt tcaaggcccc atatcagcac
     4861 tttgtggata ccctgaagtc caaggagttc gaggactcta tcctgtttag ctatcaggtg
     4921 gattctaagt tcaacaggaa gatcagcgac gccaccatct acgccacacg ccaggccaaa
     4981 gtgggcaagg acaaggccga tgagacctat gtgctgggca agatcaagga catctacaca
     5041 caggacggct atgatgcctt tatgaaaatc tacaagaagg ataagagcaa gttcctgatg
     5101 tataggcacg acccccagac atttgagaaa gtgatcgagc caatcctgga gaactacccc
     5161 aataagcaga tcaatgagaa gggcaaggag gtgccctgca accctttcct gaagtataag
     5221 gaggagcacg gctacatccg caagtattcc aagaagggca atggccctga gatcaagtcc
     5281 ctgaagtact atgactctaa gctgggcaac cacatcgaca tcacccccaa ggactctaac
     5341 aataaggtgg tgctgcagtc cgtgtctcct tggcgggccg acgtgtactt caacaagacc
     5401 accggcaagt acgagatcct gggcctgaag tatgccgacc tgcagtttga gaagggcacc
     5461 ggcacataca agatcagcca ggagaagtat aatgacatca agaagaagga gggcgtggac
     5521 agcgattccg agttcaagtt tacactgtac aagaacgatc tgctgctggt gaaggacacc
     5581 gagacaaagg agcagcagct gttccggttt ctgagcagaa ccatgcctaa gcagaagcac
     5641 tacgtggagc tgaagcccta cgacaagcag aagttcgagg gaggagaggc cctgatcaag
     5701 gtgctgggca acgtggccaa ttccggccag tgtaagaagg gcctgggcaa gtctaacatc
     5761 agcatctaca aggtgcggac cgatgtgctg ggcaatcagc acatcatcaa gaacgagggc
     5821 gataagccta agctggactt tggatccccc aagaagaaga ggaaagtctc gagcgattac
     5881 aaagacgatg acgataaggg cagcggcgca acaaacttct ctctgctgaa acaagccgga
     5941 gatgtcgaag agaatcctgg accgatggcc aagcctttgt ctcaagaaga atccaccctc
     6001 attgaaagag caacggctac aatcaacagc atccccatct ctgaagacta cagcgtcgcc
     6061 agcgcagctc tctctagcga cggccgcatc ttcactggtg tcaatgtata tcattttact
     6121 gggggacctt gtgcagaact cgtggtgctg ggcactgctg ctgctgcggc agctggcaac
     6181 ctgacttgta tcgtcgcgat cggaaatgag aacaggggca tcttgagccc ctgcggacgg
     6241 tgccgacagg tgcttctcga tctgcatcct gggatcaaag ccatagtgaa ggacagtgat
     6301 ggacagccga cggcagttgg gattcgtgaa ttgctgccct ctggttatgt gtgggagggc
     6361 taagaattcg atatcaagct tatcggtaat caacctctgg attacaaaat ttgtgaaaga
     6421 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     6481 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     6541 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     6601 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     6661 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     6721 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     6781 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     6841 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     6901 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     6961 tgggccgcct ccccgcatcg ctttaagacc aatgacttac aaggcagctg tagatcttag
     7021 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga
     7081 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     7141 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     7201 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     7261 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
     7321 tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta
     7381 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     7441 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc
     7501 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     7561 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     7621 aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt
     7681 cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     7741 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     7801 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc
     7861 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     7921 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     7981 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     8041 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     8101 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     8161 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     8221 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     8281 attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg
     8341 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     8401 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     8461 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     8521 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     8581 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     8641 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     8701 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     8761 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     8821 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     8881 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     8941 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     9001 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     9061 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     9121 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     9181 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     9241 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     9301 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     9361 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     9421 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     9481 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     9541 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     9601 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     9661 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     9721 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     9781 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     9841 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     9901 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     9961 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
    10021 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
    10081 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
    10141 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
    10201 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
    10261 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
    10321 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
    10381 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
    10441 tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag
    10501 ggaacaaaag ctggagctgc aagctt
//
