LOCUS       sequence-441843-       10115 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human codon-optimized enCjCas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10115)
  AUTHORS   Seo SY, Min S, Lee S, Seo JH, Park J, Kim HK, Song M, Baek D, Cho 
            SR, Kim HH
  TITLE     Massively parallel evaluation and computational prediction of the 
            activities and specificities of 17 small Cas9s.
  JOURNAL   Nat Methods. 2023 Jul;20(7):999-1009. doi: 
            10.1038/s41592-023-01875-2. Epub 2023 May 15.
  PUBMED    37188955
REFERENCE   2  (bases 1 to 10115)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-023-01875-2"; journalName: "Nat Methods"; date: 
            "2023-07"; volume: "20"; issue: "7"; pages: "999-1009"
COMMENT     Sequence Label: pLenti6.3-enCjCas9-BlastR
FEATURES             Location/Qualifiers
     source          1..10115
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1079..1312
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1497..1541
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     enhancer        1820..2123
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2124..2327
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2278..2298
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2324..2348
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        2399..2417
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      2425..2434
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2437..2457
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5437..5457
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5464..5487
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5497..5553
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5554..5952
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    5977..6565
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6030..6050)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6448..6459)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6636..6869
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6941..7062
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6978..6997)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7032..7051
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7102..7237
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(7258..7276)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7286..7303)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7286..7302)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7295..7317)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7444..7899
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7531..7550)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7741..7762
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7925..8029
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8030..8098,8099..8890)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8248..8267)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9061..9649
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9550..9569
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9803..9820
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9937..9958
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9973..9978,9979..9996,9997..10003)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10011..10027
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10016..10038
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10035..10051
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10035..10051
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10070..10090
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10072..10090
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccggccgc tgatcttcag acctggagga ggagatatga
      961 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
     1021 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
     1081 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
     1141 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
     1201 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
     1261 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
     1321 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
     1381 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
     1441 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
     1501 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
     1561 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
     1621 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
     1681 cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag
     1741 aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc
     1801 gataagcttg ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     1861 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     1921 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     1981 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     2041 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     2101 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
     2161 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
     2221 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
     2281 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc
     2341 gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga caccgactta
     2401 atacgactca ctatagggag agccgccacc atggcaccga agaagaagcg taaagtcgga
     2461 atccacggag ttcctgcggc agccagaatc ctggcctttg acatcggcat cagctccatc
     2521 ggctgggcct tcagcgagaa cgacgagctg aaggattgcg gcgtgagaat cttcaccaag
     2581 gtggagaatc ctaagacagg cgagtctctg gccctgccac ggagactggc ccggagcgcc
     2641 aggaagcgct atgccaggcg caaggcaaga ctgaaccacc tgaagcacct gatcgccaac
     2701 gagtttaagc tgaattacga ggactatcag tctttcgatg agagcctggc caaggcctac
     2761 aagggcagcc tgatctcccc ttatgagctg cggtttagag ccctgaacga gctgctgagc
     2821 aagcaggact tcgccagagt gatcctgcac atcgccaagc ggagaggcta cgacgacatc
     2881 aagaacagcg acgataagga gaagggcgcc atcctgaagg ccatcaagca gaacgaggag
     2941 aagctggcca attaccagtc cgtgggcgag tacctgtata aggagtattt tcagaagttc
     3001 aaggagaact ctaaggagtt taccaacgtg cggaataaga aggagtccta cgagagatgc
     3061 atcgcccagt cttttctgaa ggatgagctg aagctgatct tcaagaagca gagggagttc
     3121 ggcttttcct tctctaagaa gttcgaggag gaggtgctgt ccgtggcctt ttataagcgc
     3181 gccctgaagg acttcagcca cctggtgggc aactgttcct tctttaccga tgagaagagg
     3241 gcccccaaga acagccctct ggcctttatg ttcgtggccc tgacacgcat catcaacctg
     3301 ctgaacaatc tgaagaatac cgagggcatc ctgtacacaa aggacgatct gaacgccctg
     3361 ctgaatgagg tgctgaagaa cggcaccctg acatacaagc agaccaagaa gctgctgggc
     3421 ctgagcgacg attatgagtt taagggcgag aagggcacat actttatcga gttcaagaag
     3481 tataaggagt tcatcaaggc cctgggcgag cacaacctgt cccaggacga tctgaatgag
     3541 atcgccaagg acatcaccct gatcaaggat gagatcaagc tgaagaaggc cctggccaag
     3601 tacgacctga accagaatca gatcgatagc ctgtccaagc tggagtttaa ggaccacctg
     3661 aacatcagct tcaaggccct gaagctggtg acacccctga tgctggaggg caagaagtat
     3721 gatgaggcct gtaacgagct gaatctgaag gtggccatca atgaggacaa gaaggatttt
     3781 ctgccagcct tcaacgagac ctactataag gacgaggtga caaatcccgt ggtgctgagg
     3841 gccatcaagg agtaccgcaa ggtgctgaac gccctgctga agaagtatgg caaggtgcac
     3901 aagatcaaca tcgagctggc cagggaggtc ggcaagaatc actctcagcg cgccaagatc
     3961 gagaaggagc agaacgagaa ttacaaggcc aagaaggatg ccgagctgga gtgcgagaag
     4021 ctgggcctga agatcaactc caagaatatc ctgaagctga ggctgtttaa ggagcagaag
     4081 gagttctgtg cctactctgg cgagaagatc aagatcagcg acctgcagga tgagaagatg
     4141 ctggagatcg accacatcta cccctattct cgcagctttg acgattctta tatgaacaag
     4201 gtgctggtgt tcaccaagca gaaccaggag aagctgaatc agacaccttt tgaggccttc
     4261 ggcaacgata gcgccaagtg gcagaagatc gaggtgctgg ccaagaatct gccaaccaag
     4321 aagcagaaga gaatcctgga caagaactac aaggataagg agcagaagaa cttcaaggac
     4381 agaaacctga atgatacccg gtatatcgcc agactggtgc tgaattacac aaaggactat
     4441 ctggatttcc tgcctctgtc cgacgatgag aacaccaagc tgaatgacac acagaagggc
     4501 tctaaggtgc acgtggaggc caagagcgga atgctgacct ccgccctgag gcacacatgg
     4561 ggcttcagcg ccaaggacag aaacaatcac ctgcaccacg ccatcgatgc cgtgatcatc
     4621 gcctacgcca acaattccat cgtgaaggcc ttttctgact tcaagaagga gcaggagtcc
     4681 aactctgccg agctgtacgc caagaagatc agcgagctgg attataagaa taagaggaag
     4741 ttctttgagc cattttccgg cttccgccag aaggtgctgg acaagatcga tgagattttc
     4801 gtgagcaagc cagagaggaa gaagccatcc ggcgccctgc acgaggagac ctttcgcaag
     4861 gaggaggagt tctaccagtc ctatggcggc aaggagggcg tgctgaaggc cctggagctg
     4921 ggcaagatcc ggaaggtgaa cggcaagatc gtgaagaatg gcgacatgtt tagagtggac
     4981 atcttcaagc acaagaagac caacaagttt tacgccgtgc ccatctatac aatggacttc
     5041 gccctgaagg tgctgcctaa taaggccgtg gccaggagca agaagggcga gatcaaggac
     5101 tggattctga tggatgagaa ctacgagttt tgcttctctc tgtataagga cagcctgatc
     5161 ctgatccaga ccaagaaaat gcaggagccc gagttcgtgt actataatgc cttcacctct
     5221 agcacagtgt ccctgatcgt gtctaagcac gacaacaagt ttgagacact gtccaagaat
     5281 cagaagatcc tgttcaagaa cgccaatgag aaggaagtga tcgccaagtc tatcggcatc
     5341 cagaacctga aggtgttcga gaagtacatc gtgagcgccc tgggagaggt gaccaaggcc
     5401 gagtttaggc agcgcgagga tttcaagaag ggatccccca agaagaagag gaaagtctcg
     5461 agcgattaca aagacgatga cgataagggc agcggcgcaa caaacttctc tctgctgaaa
     5521 caagccggag atgtcgaaga gaatcctgga ccgatggcca agcctttgtc tcaagaagaa
     5581 tccaccctca ttgaaagagc aacggctaca atcaacagca tccccatctc tgaagactac
     5641 agcgtcgcca gcgcagctct ctctagcgac ggccgcatct tcactggtgt caatgtatat
     5701 cattttactg ggggaccttg tgcagaactc gtggtgctgg gcactgctgc tgctgcggca
     5761 gctggcaacc tgacttgtat cgtcgcgatc ggaaatgaga acaggggcat cttgagcccc
     5821 tgcggacggt gccgacaggt gcttctcgat ctgcatcctg ggatcaaagc catagtgaag
     5881 gacagtgatg gacagccgac ggcagttggg attcgtgaat tgctgccctc tggttatgtg
     5941 tgggagggct aagaattcga tatcaagctt atcggtaatc aacctctgga ttacaaaatt
     6001 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     6061 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     6121 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     6181 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     6241 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     6301 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     6361 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
     6421 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     6481 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     6541 atctcccttt gggccgcctc cccgcatcgc tttaagacca atgacttaca aggcagctgt
     6601 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     6661 aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     6721 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     6781 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     6841 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta
     6901 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta
     6961 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     7021 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct
     7081 atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     7141 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     7201 ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct
     7261 atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     7321 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     7381 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     7441 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     7501 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     7561 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     7621 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     7681 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     7741 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     7801 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     7861 ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa
     7921 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     7981 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     8041 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     8101 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     8161 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     8221 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     8281 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     8341 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     8401 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     8461 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     8521 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     8581 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     8641 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     8701 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     8761 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     8821 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     8881 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     8941 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     9001 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     9061 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     9121 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     9181 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     9241 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     9301 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     9361 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     9421 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     9481 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     9541 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     9601 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     9661 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     9721 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     9781 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     9841 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     9901 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     9961 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
    10021 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
    10081 tcactaaagg gaacaaaagc tggagctgca agctt
//
