LOCUS       sequence-441846-        7928 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor plasmid of hAT-11_AMi Transposon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7928)
  AUTHORS   Zhang T, Tan S, Tang N, Li Y, Zhang C, Sun J, Guo Y, Gao H, Cai Y, 
            Sun W, Wang C, Fu L, Ma H, Wu Y, Hu X, Zhang X, Gee P, Yan W, Zhao 
            Y, Chen Q, Guo B, Wang H, Zhang YE
  TITLE     Heterologous survey of 130 DNA transposons in human cells highlights
            their functional divergence and expands the genome engineering 
            toolbox.
  JOURNAL   Cell. 2024 Jul 11;187(14):3741-3760.e30. doi: 
            10.1016/j.cell.2024.05.007. Epub 2024 Jun 5.
  PUBMED    38843831
REFERENCE   2  (bases 1 to 7928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2024.05.007"; journalName: "Cell"; date: 
            "2024-07-11- 11"; volume: "187"; issue: "14"; pages: "3741-3760.e30"
COMMENT     Sequence Label: pMV-hAT-11_AMi
FEATURES             Location/Qualifiers
     source          1..7928
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     94..112
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     121..138
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     122..138
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1095..1594
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(1130..1153)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     1527..1546
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      1608..1617
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1614..2213
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MGTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     2113..2133
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             2220..2276
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(2277..2279,2280..2282,2283..2996)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2277..2279,2280..2282,2283..2993)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2322..2343)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2583..2602)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2930..2951
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(3018..3035)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3024..3248
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6031..6047)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6031..6047)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6059..6076)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6230..6818)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6310..6329)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6989..7780,7781..7849))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLRNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERKRQIAEIGAS
                     LIKHW"
     primer_bind     7612..7631
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(7850..7928,1..26))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
       61 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
      121 tgtaaaacga cggccagtcg aaccacgcaa tgcgtctcga tccgcagtgt cttgcgtctc
      181 tcaattgctg tatagtaggg ctgtgcgaaa cttcggcggc cgtttcgttt cggaggtgtt
      241 tcggcccgtt tcggcgcccg aaacaccgaa tccgaaacga aacggaaggc tccgaaacag
      301 ctccgaaacg aaacggaacc atccgaaacg tttcggaatg tttcggaggc gggcttgcgg
      361 ggaaacagaa actcagaaga gggaggggga agaggggggg aaggactgct ctgatattga
      421 catctgtagc tggccagtgt ctgcgatctg tccctgaggt cccttccaat cccagtgctc
      481 tgggggaggg acggagaaac agcatataag gctgtttgtg aactgctctc tgccattttg
      541 cgagatctgt ctcttgcagc acgagcagca gagcgcagca tctgaagggt ctaggcttgc
      601 agcttggtag tccgttgcta ccaagttgta gccagccatt agccaatcct ttcccactta
      661 cccttgccta ccatccctat tgttaatccc tttcaaatat ttcttcccca aaaatcctgt
      721 tatttattct tgttattttt tttccacccc aaacaaaaag aaagctgtgc tttgcctggc
      781 agtgtctcat cactgcgcag gaaagcacta cacatataca tcacacagac gcacattttc
      841 cagcacacca aacatgaagc gtgctggaaa ggcaggtgcc aggtcttctg gcaggggagc
      901 gagagggggc agagggagag ctgtaagtcc ccccccccaa acttagacgc accctcttcc
      961 ctagcaggca tgaggcttct gctgctagtg gcagcaagga ggtgggagca gtgtctgcac
     1021 ctgcaccacc tccactaaca ccagcaataa taataacccc cagcaccacc agcaccggtg
     1081 ctagcaattc taccgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt
     1141 agcagccccg ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac
     1201 atccaccggt aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac
     1261 tcctccccta gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca
     1321 aatggaagta gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa
     1381 gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca
     1441 gaggctggga aggggtgggt ccgggggcgg gctcaggggc gggctcaggg gcggggcggg
     1501 cgcccgaagg tcctccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc
     1561 gctgttctcc tcttcctcat ctccgggcct ttcgacctgc agccaacgcc accatgggga
     1621 ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtcccccgg gccgtacgca
     1681 ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgac ccggaccgcc
     1741 acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg
     1801 gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg
     1861 tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc
     1921 ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg
     1981 cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg
     2041 ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga
     2101 cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg
     2161 tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt gccatcgatg
     2221 ccactaactt ctccctgttg aaacaagcag gggatgtcga agagaatccc gggccaatgg
     2281 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     2341 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
     2401 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     2461 tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc
     2521 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
     2581 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     2641 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     2701 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca
     2761 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
     2821 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     2881 tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     2941 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaattaa
     3001 ttaagctcgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     3061 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     3121 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     3181 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
     3241 ctctatggct tctgaggcgc gccctcgagg tgccaccctc ctcactccgt ccgcacctat
     3301 ttcctttttt atttttttta accatttaat gccccgctct cagagctgga ggcggcactc
     3361 actgcatcac cagccagcag gggaagaaca tctccctgct gagctcccgt gccaggacga
     3421 acttggaggt atgggctggg gctatgggga aggaggaggc cccaggtcct gggcatgacc
     3481 taaaactgca ccctgccagg gtcgggaggc ctggtgggac tgccggtggc gcggcagccc
     3541 ccaggaaatg ccaggaccgc gaacctccct ggtgaaaggc gggtaggagg cgccttataa
     3601 cctccagcca ccacacgcat gcacagtcct ggctagggaa agcccagacc tgtaagatct
     3661 ttgagaggcc tgaggcttgc tggttgcagt ggagttgtga ggctccaggt gagcttagct
     3721 gcctgggatg ccatctgcga ggcatgggcc gcagtgtagg ggaggtacgg agctgcggaa
     3781 cggatgtcac ccctagacat caggggccta aatgggcagc ctcccgcctg ggtaacatct
     3841 gaatcggttt tccatcaagg catggcagag aaagaggggc gggcggttgg cccagaaacg
     3901 ggtgggcggg ctgagatctc accggcaccg ggaaggcccc ctgttacagg ctaccatccc
     3961 tgctgctttc atcccgctcc gccttaacac acacaaggca gacaaggttc tttgggccaa
     4021 tctgatatct tttattagac caactgaata gttggagaaa tatatcttag caagctttcg
     4081 ggtttaaaac ccttcgtcag gctgaggaag catctgcagt tggtgcgtgc tcttcctgga
     4141 tggaaggaat agtaaagaag ccagaggctg gcatgcaatg caggcaagaa agccagtcag
     4201 tgaaaatgta aattgaggcg tcagggggtg agggacaggc tgggggcggg ggatgtagca
     4261 ggtaaaagtg gagaggtacc tggggagtca gatgtcgggc aggttatagt gtgtcataaa
     4321 tccaatgtct atattgagtc catgagtttc tgtatctagg aggttgatga agtgaagttc
     4381 ataggctcgt ctgtgaaaag tggtttgtaa attccctttg aggatcagga ctgagagact
     4441 ggagacagag tggctttctt gtgagaaatg tgcccccaca ggtagttggg tattcttgtc
     4501 tgtaatagat ttccggcgtg cattcattct ggtgcgcagt tgttgtttgg tgtctcctac
     4561 gtattttcca tcagggcatt tggtgcaccg gatgagatat ataacgtttc tggaggcgca
     4621 gctgtaagat ccaggagtgg tgatggttct gttgtggggt gtagtaattg tgggggtact
     4681 ggagatatgt tggcaggttt tgcatttctt gtcatggcat ggtctggatc cattcggcgt
     4741 gttttgggct gtaggaagtt tgcttctggt gatgaggtta gcgaggttcg gtgcttgttt
     4801 gaatttacaa accacttttc acagacgagc ctatgaactt cacttcatca acctcctaga
     4861 tacagaaact catggactca atatagacat tggatttatg acacgctata acctgcccga
     4921 catctgactc cccaggtacc tctccacttt tacctgctac atccccctcc ccccgccccg
     4981 cctgtccctc accccctgac acctcaattt acattttcac tgactggctt tcttgcctgc
     5041 attgcatgcc agcctctggc ttctttacta ttccttccat ccaggaagag cacacacacc
     5101 aactgcagat gcttcctcag cctgacgaag ggtttttaaa cccgaaagct tgctaagata
     5161 tatttctcca actattcagt tggtctaata aaagatatca gaggtaccct cgactggtcc
     5221 gtgctggggc caggacccaa agcggggtca aagggccagg ggcccaccgc gaggaatgcc
     5281 cagagcaaga ggctcgggat tccgactggc ctgaaggcgg gaccagggcc taagggagag
     5341 ccaaaggtcc cagagagggg accacagctg gagggggtga aggatgcaat gtgcgaggcg
     5401 tgggccgcag tgtaggggag gtacggagct gcggaatgga tgtcacccca agacacctaa
     5461 atgggcagcc tcccgcccgg gaaacatctg aatcagtttt ccatcaaggc gtggcaggaa
     5521 aaagaggggc gggcggttgg cccagaaaca ggtgggcagg cggaaatcta accggcaccg
     5581 ggaaggcccc ctgttacagg ctaccatccc tgctggtttc atcccgctgc gccttaacac
     5641 acacacaggt cacacatgtc acgagttttg ctttgaacag tttgaggtgc agtttcacag
     5701 aaacccctgc acctatctcc ttgaaacctg gcaggcttca tgccctcaga aggggctacc
     5761 atccctgcag ttttcatccg aatcaggcaa gaaatgacaa agttataggc attttagtga
     5821 ttcctcatta tagcctatgg ccgaatcact gaaacagcgt cgaaactctg aaacggattc
     5881 ggtcgaatcg aaacggaaca gtgatccgaa acactgaaat gaatcactgc gctccaaaac
     5941 ggccgaatcc gaaactgaat caaaacgtag ccgtttcgca cggccctact gtataggggc
     6001 ccagagacgg agtcactgcc aaccgagacg gtcatagctg tttcctgtgt gccgcttcct
     6061 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     6121 aggcggtaat acggttaccc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     6181 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     6241 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     6301 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     6361 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     6421 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     6481 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     6541 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     6601 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     6661 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     6721 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     6781 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     6841 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     6901 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     6961 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     7021 cagcgatctg tctctttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     7081 cgatacggga gggcttacca tctggcccca gtgctgcaat aataccgcgg gacccacgct
     7141 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     7201 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     7261 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat cgctacaggc atcgtggtat
     7321 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     7381 catgatcccc catgttgcgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     7441 gaagtaagtt ggccgccgtg ttatcactca tggttatggc agcactacat aattctctta
     7501 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     7561 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     7621 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     7681 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     7741 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     7801 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     7861 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     7921 gtatttag
//
