LOCUS       sequence-441847-       10409 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human codon-optimized Nm2Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10409)
  AUTHORS   Seo SY, Min S, Lee S, Seo JH, Park J, Kim HK, Song M, Baek D, Cho 
            SR, Kim HH
  TITLE     Massively parallel evaluation and computational prediction of the 
            activities and specificities of 17 small Cas9s.
  JOURNAL   Nat Methods. 2023 Jul;20(7):999-1009. doi: 
            10.1038/s41592-023-01875-2. Epub 2023 May 15.
  PUBMED    37188955
REFERENCE   2  (bases 1 to 10409)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-023-01875-2"; journalName: "Nat Methods"; date: 
            "2023-07"; volume: "20"; issue: "7"; pages: "999-1009"
COMMENT     Sequence Label: pLenti6.3-Nm2Cas9-BlastR
FEATURES             Location/Qualifiers
     source          1..10409
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1079..1312
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1497..1541
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     enhancer        1820..2123
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2124..2327
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2278..2298
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2324..2348
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        2399..2417
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      2425..2434
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2437..2457
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5731..5751
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5758..5781
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5791..5847
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5848..6246
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    6271..6859
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6324..6344)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6742..6753)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6930..7163
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7235..7356
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7272..7291)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7326..7345
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7396..7531
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(7552..7570)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7580..7597)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7580..7596)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7589..7611)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7738..8193
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7825..7844)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8035..8056
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8219..8323
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8324..8392,8393..9184)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8542..8561)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9355..9943
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9844..9863
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10097..10114
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10231..10252
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10267..10272,10273..10290,10291..10297)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10305..10321
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10310..10332
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10329..10345
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10329..10345
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10364..10384
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10366..10384
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccggccgc tgatcttcag acctggagga ggagatatga
      961 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
     1021 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
     1081 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
     1141 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
     1201 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
     1261 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
     1321 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
     1381 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
     1441 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
     1501 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
     1561 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
     1621 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
     1681 cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag
     1741 aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc
     1801 gataagcttg ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     1861 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     1921 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     1981 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     2041 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     2101 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
     2161 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
     2221 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
     2281 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc
     2341 gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga caccgactta
     2401 atacgactca ctatagggag agccgccacc atggcaccga agaagaagcg taaagtcgga
     2461 atccacggag ttcctgcggc agccgccttc aagcctaacc caatcaatta catcctgggc
     2521 ctggacatcg gaatcgcctc cgtgggatgg gctatggtgg agatcgacga ggaggagaat
     2581 cctatccggc tgatcgatct gggcgtgaga gtgtttgaga gggccgaggt gccaaagacc
     2641 ggcgatagcc tggctatggc ccggcggctg gcccggagcg tgaggcgcct gacacggaga
     2701 agggcccacc gcctgctgcg ggcccgccgg ctgctgaaga gagagggcgt gctgcaggca
     2761 gcagacttcg atgagaatgg cctgatcaag agcctgccaa acaccccctg gcagctgaga
     2821 gccgccgccc tggacaggaa gctgacaccc ctggagtggt ctgccgtgct gctgcacctg
     2881 atcaagcacc gcggctacct gagccagcgg aagaacgagg gagagacagc agacaaggag
     2941 ctgggcgccc tgctgaaggg agtggccaac aatgcccacg ccctgcagac cggcgatttc
     3001 aggacacctg ccgagctggc cctgaataag tttgagaagg agtccggcca catcagaaac
     3061 cagaggggcg actatagcca caccttctcc cgcaaggatc tgcaggccga gctgatcctg
     3121 ctgttcgaga agcagaagga gtttggcaat ccacacgtga gcggaggcct gaaggaggga
     3181 atcgagaccc tgctgatgac acagaggcct gccctgtccg gcgacgccgt gcagaagatg
     3241 ctgggacact gcaccttcga gcctgcagag ccaaaggccg ccaagaacac ctacacagcc
     3301 gagcggttta tctggctgac aaagctgaac aatctgagaa tcctggagca gggctccgag
     3361 aggcccctga ccgacacaga gagggccacc ctgatggatg agccttaccg gaagtctaag
     3421 ctgacctacg cccaggccag aaagctgctg ggcctggagg acaccgcctt ctttaagggc
     3481 ctgagatacg gcaaggataa tgccgaggcc tccacactga tggagatgaa ggcctatcac
     3541 gccatctctc gcgccctgga gaaggagggc ctgaaggaca agaagtcccc cctgaacctg
     3601 agcagcgagc tgcaggatga gatcggcacc gccttctctc tgtttaagac cgacgaggac
     3661 atcacaggcc gcctgaagga cagggtgcag cctgagatcc tggaggccct gctgaagcac
     3721 atctctttcg ataagtttgt gcagatcagc ctgaaggccc tgagaaggat cgtgccactg
     3781 atggagcagg gcaagcggta cgacgaggcc tgcgccgaaa tctacggcga tcactatggc
     3841 aagaagaaca cagaggagaa aatctacctg ccccctatcc ccgccgacga gatcagaaat
     3901 cctgtggtgc tgagggccct gtcccaggca agaaaagtga tcaacggagt ggtgcgccgg
     3961 tacggctctc cagccagaat ccacatcgag accgccagag aggtcggcaa gagcttcaag
     4021 gaccggaagg agatcgagaa gagacaggag gagaatcgca aggatcggga gaaggctgcc
     4081 gccaagttta gggagtactt ccctaacttt gtgggcgagc caaagtctaa ggacatcctg
     4141 aagctgcgcc tgtacgagca gcagcacggc aagtgtctgt atagcggcaa ggagatcaat
     4201 ctggtgcggc tgaacgagaa gggctatgtg gagatcgatc acgccctgcc tttctccaga
     4261 acctgggacg attcttttaa caataaggtg ctggtgctgg gcagcgagaa ccagaataag
     4321 ggcaatcaga caccatacga gtatttcaat ggcaaggaca actccaggga gtggcaggag
     4381 ttcaaggccc gcgtggagac cagccggttt ccaaggagca agaagcagag aatcctgctg
     4441 cagaagttcg acgaggatgg ctttaaggag tgcaacctga atgacaccag atacgtgaac
     4501 cggttcctgt gccagtttgt ggccgatcac atcctgctga ccggcaaggg caagagaagg
     4561 gtgttcgcct ctaatggcca gatcacaaac ctgctgaggg gattttgggg actgaggaag
     4621 gtgcgggcag agaatgacag acaccacgcc ctggatgcag tggtggtggc atgcagcacc
     4681 gtggcaatgc agcagaagat cacaagattc gtgaggtata aggagatgaa cgcctttgac
     4741 ggcaagacca tcgataagga gacaggcaag gtgctgcacc agaagaccca cttcccccag
     4801 ccttgggagt tctttgccca ggaagtgatg atccgggtgt tcggcaagcc agacggcaag
     4861 cctgagtttg aggaggccga taccccagag aagctgagga cactgctggc cgagaagctg
     4921 tctagccgcc ccgaggcagt gcacgagtac gtgaccccac tgttcgtgtc ccgcgccccc
     4981 aatcggaaga tgtctggcgc ccacaaggac acactgagaa gcgccaagag gtttgtgaag
     5041 cacaacgaga agatcagcgt gaagagagtg tggctgaccg agatcaagct ggccgatctg
     5101 gagaacatgg tgaattacaa gaacggcagg gagatcgagc tgtatgaggc cctgaaggca
     5161 cgcctggagg cctacggcgg aaatgccaag caggccttcg acccaaagga taaccccttt
     5221 tataagaagg gaggacagct ggtgaaggcc gtgcgggtgg agaagaccca ggagagcggc
     5281 gtgctgctga ataagaagaa cgcctacaca atcgccgaca atggcgatat ggtgagagtg
     5341 gacgtgttct gtaaggtgga taagaagggc aagaatcagt actttatcgt gcctatctat
     5401 gcctggcagg tggccgagaa catcctgcca gacatcgatt gcaagggcta cagaatcgac
     5461 gatagctata cattctgttt ttccctgcac aagtatgacc tgatcgcctt ccagaaggat
     5521 gagaagtcca aggtggagtt tgcctactat atcaattgcg actcctctaa cggcaggttc
     5581 tacctggcct ggcacgataa gggcagcaag gagcagcagt ttcgcatctc cacccagaat
     5641 ctggtgctga tccagaagta tcaggtgaac gagctgggca aggagatcag gccatgtcgg
     5701 ctgaagaagc gcccacccgt gcggggatcc cccaagaaga agaggaaagt ctcgagcgat
     5761 tacaaagacg atgacgataa gggcagcggc gcaacaaact tctctctgct gaaacaagcc
     5821 ggagatgtcg aagagaatcc tggaccgatg gccaagcctt tgtctcaaga agaatccacc
     5881 ctcattgaaa gagcaacggc tacaatcaac agcatcccca tctctgaaga ctacagcgtc
     5941 gccagcgcag ctctctctag cgacggccgc atcttcactg gtgtcaatgt atatcatttt
     6001 actgggggac cttgtgcaga actcgtggtg ctgggcactg ctgctgctgc ggcagctggc
     6061 aacctgactt gtatcgtcgc gatcggaaat gagaacaggg gcatcttgag cccctgcgga
     6121 cggtgccgac aggtgcttct cgatctgcat cctgggatca aagccatagt gaaggacagt
     6181 gatggacagc cgacggcagt tgggattcgt gaattgctgc cctctggtta tgtgtgggag
     6241 ggctaagaat tcgatatcaa gcttatcggt aatcaacctc tggattacaa aatttgtgaa
     6301 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     6361 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     6421 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     6481 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     6541 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     6601 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     6661 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     6721 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     6781 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     6841 ctttgggccg cctccccgca tcgctttaag accaatgact tacaaggcag ctgtagatct
     6901 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
     6961 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
     7021 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     7081 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     7141 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata
     7201 acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg
     7261 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     7321 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg
     7381 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     7441 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     7501 cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg
     7561 agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
     7621 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     7681 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg
     7741 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     7801 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     7861 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     7921 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     7981 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     8041 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     8101 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     8161 cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg
     8221 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     8281 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     8341 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     8401 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     8461 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     8521 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     8581 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     8641 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     8701 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     8761 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     8821 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     8881 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     8941 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     9001 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     9061 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     9121 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     9181 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     9241 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     9301 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     9361 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     9421 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     9481 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     9541 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     9601 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     9661 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     9721 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     9781 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     9841 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     9901 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     9961 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
    10021 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
    10081 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
    10141 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
    10201 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
    10261 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
    10321 atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta
    10381 aagggaacaa aagctggagc tgcaagctt
//
