LOCUS       sequence-441870-        8293 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor plasmid of hAT-6_AMi Transposon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8293)
  AUTHORS   Zhang T, Tan S, Tang N, Li Y, Zhang C, Sun J, Guo Y, Gao H, Cai Y, 
            Sun W, Wang C, Fu L, Ma H, Wu Y, Hu X, Zhang X, Gee P, Yan W, Zhao 
            Y, Chen Q, Guo B, Wang H, Zhang YE
  TITLE     Heterologous survey of 130 DNA transposons in human cells highlights
            their functional divergence and expands the genome engineering 
            toolbox.
  JOURNAL   Cell. 2024 Jul 11;187(14):3741-3760.e30. doi: 
            10.1016/j.cell.2024.05.007. Epub 2024 Jun 5.
  PUBMED    38843831
REFERENCE   2  (bases 1 to 8293)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2024.05.007"; journalName: "Cell"; date: 
            "2024-07-11- 11"; volume: "187"; issue: "14"; pages: "3741-3760.e30"
COMMENT     Sequence Label: pMV-hAT-6_AMi
FEATURES             Location/Qualifiers
     source          1..8293
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     94..112
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     121..138
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     122..138
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2373..2872
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2408..2431)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2805..2824
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      2886..2895
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2892..3491
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MGTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     3391..3411
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             3498..3554
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(3555..3557,3558..3560,3561..4274)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3555..3557,3558..3560,3561..4271)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3600..3621)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3861..3880)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4208..4229
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4296..4313)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4302..4526
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6396..6412)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6396..6412)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6424..6441)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6595..7183)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6675..6694)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7354..8145,8146..8214))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLRNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERKRQIAEIGAS
                     LIKHW"
     primer_bind     7977..7996
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(8215..8293,1..26))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
       61 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
      121 tgtaaaacga cggccagtcg aaccacgcaa tgcgtctcga tccgcagtgt cttgcgtctc
      181 tcaattgcta tatagtaggg ctgtgcgaaa tttcgccggc cgtttcgttt cgacgctgtt
      241 tcgacctgtt tcaagattga aacagcgaaa ttgaaactaa acaaggcagg tcgaaacatt
      301 tcgaaagttt tgaagtgttt cgactctgaa gctggggctg ggctttcttg cagggaaaca
      361 gaaagtaaag agagggaggg ggagaggggg gggaaggatg ctctgatatt gactgtctag
      421 ctggccagtg actgtcatct ttccctgagg tcccttccaa tcccagtgct ctgggggagg
      481 gatggaaaaa catcataaaa agcagcattg tttgaactgc ggctttctgc cttggggtca
      541 ttactgagct gtcttccaga gctagcagcg tcagacacaa aggctactac tggcctgctt
      601 gccaccctag caagtgagat tctccggtac tttttgctct agagggattc gctatacctt
      661 gctattattt tattattata ttcattgatt cattcttttc aatttatatc tctcttttgc
      721 ttagcccccc gattcttttc ggaatctgtg cttttccaca caatgtgaat ccattcactg
      781 tacagggaag cctacagcat acaatcatat gcatcctaaa atatagaatt tatataggaa
      841 aacacaaaac attcatatat ttatgttatt tattagtata gtacatacaa caatacatac
      901 attacataag tacacattca tacaattatt tcatttacat atatcacata aatcagatag
      961 ataacattag cagaagagga agattttggg cacaagcaaa caagacacag aaacattacc
     1021 atgaagcgtg ctggaaaggc agctggcagg ccttcaggga ggggtgggag agggggtaga
     1081 ggggtagggg gaagctagag ctgcaagttt ccaccccccc acccccaaac tgagatgcag
     1141 cctctttcct acaactagca ggcatgaggc ttctgctgct agtcaggcag caaggagagg
     1201 ggagcagtgc ctgtgcctga gcctgcatcc cctccaaaag cacagccata acccccacca
     1261 ccaagcagca gcaccagcat gacagtctcc tccaccccca tttcacttcc catgctgagc
     1321 ctcccagtgc cagaggaaga gctggagctg gaaccagatc tgagcgccct agcaagggaa
     1381 attctgggga aggagaggga atcatctgag tttgtgctcc acacccctca agtttcccct
     1441 agagccaggt ctccttcccc agaaggcact tcggaggagg ttgaggtaga ggttgtggag
     1501 gcaagtggct cagctgagtc cgctcctcct gttgctgtgc ctctgcctgc tgaggagggg
     1561 gaaagggcag catccacaca tgcacctgca ccccagaagc gagcaggtag tgtggtctgg
     1621 gatcattttg agctggcaga tgatcccacg tatgctatct gcctgcactg ccaaggcaaa
     1681 accagccggg gcaagggaac gaaacacatg agcaccacgg ggatgctgat gcatctcgca
     1741 ggcaccaccc ccttgccctt gctcctcctc agcctggcac cagtgggagc atgcccaaaa
     1801 ggaagtcccc cgcttgctcc aaagcccccg tccccccaaa gcagaggcag gccaccctgg
     1861 agcagtgggg gaaaggtgga gagaaagcgg ggcatgttgc aaaggcgagc gagatcaccc
     1921 agagcattgg ggagatgctt ctgtggatgg ccagcccttc tccttagttg agcagccagg
     1981 gttcaggcag ctcatggcac tcatggcccc atcataccaa gtgcccgcat gcaccacctt
     2041 cagcaggacg gtggtgccct ccctgtatga ggcatgcagg gagtacttga gggaggagct
     2101 gcacaaggca ggtccgcagg tggccttaca cttcacctcg gacatctgga gcagccgggg
     2161 tggagatcac gcatacctct ccctcacagg gcactggtgc gaccagtcag gccatcggtg
     2221 ggctctcctt caagccaagg tgatggatga gtcccacacg gcaacagaga tcatgggggc
     2281 catgaaccac ttggtgcagg ggtggctcgt tgggcagggt gagctcaccc gcgggttcat
     2341 ggtcaccgac aaccggtgct agcaattcta ccgggtaggg gaggcgcttt tcccaaggca
     2401 gtctggagca tgcgctttag cagccccgct gggcacttgg cgctacacaa gtggcctctg
     2461 gcctcgcaca cattccacat ccaccggtag gcgccaaccg gctccgttct ttggtggccc
     2521 cttcgcgcca ccttctactc ctcccctagt caggaagttc ccccccgccc cgcagctcgc
     2581 gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact agtctcgtgc agatggacag
     2641 caccgctgag caatggaagc gggtaggcct ttggggcagc ggccaatagc agctttgctc
     2701 cttcgctttc tgggctcaga ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg
     2761 gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct gcacgcttca
     2821 aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt cgacctgcag
     2881 ccaacgccac catggggacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg
     2941 tcccccgggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca
     3001 ccgtcgaccc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc
     3061 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct
     3121 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg
     3181 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc
     3241 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg
     3301 gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg
     3361 tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct
     3421 tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca
     3481 agcccggtgc catcgatgcc actaacttct ccctgttgaa acaagcaggg gatgtcgaag
     3541 agaatcccgg gccaatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
     3601 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
     3661 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
     3721 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc
     3781 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     3841 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     3901 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     3961 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
     4021 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca
     4081 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     4141 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc
     4201 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     4261 agctgtacaa gtaattaatt aagctcgctg atcagcctcg actgtgcctt ctagttgcca
     4321 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     4381 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     4441 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     4501 tgctggggat gcggtgggct ctatggcttc tgaggcgcgc cctcgaggtg ccaccctcct
     4561 cactccatcc gcacctattt ccttttttca ttttttaaaa ccatttaatg ccccgctctc
     4621 agagctggag ggggcactca ctgcatcacc agccagcagg ggaagaacat gtccctgctg
     4681 agctcccgtg ccaggatgaa cttggaggta tggggctggg gctatgggga aggaggaggc
     4741 cccaggtcct gggcatgacc actaaaactg cactctgcca gggtcgggag gcctggtggg
     4801 actgccggcg gcgcggcagc cccaggaaat gccaggaccg aggatctccc tggtgaaagg
     4861 tgggtaggag gtgccataac ctccagccac cacatgcaca catgcacaca gtcctggctg
     4921 gggaaagccc agacccgtca cgtctttgac aggcctgagg cttgctggtt gcagtggagt
     4981 tgtgaggctc caggtgagct cagcttagct gcctgggatg ccagctttcg ggttgaaaaa
     5041 ccctttgtca ggctgaggaa gcacctgcag ttggtgtgtg tgctgttcct ggatgcactt
     5101 ttcacagatg agcctatgaa ctgcacttca tcaacctcct aggtacagaa acccatggac
     5161 tcaatataga cattggaatt ctgacacact acaacctgcc ggacatctga ctccccaggt
     5221 acctctccac ttttacctgc gctgctacat ccccttcccc ccccacccca gcctgtccct
     5281 caccccctga cgcctccatt tccattttcg ctgactggct ttcttgcctg cattgcatgc
     5341 cagcctctgg cttctttact attccttcca tccaggaaca gcacacacac caactgcagg
     5401 tgcttcctca gcctgacaaa gggtttttca acccaaaagc ttgctaagat atatttctct
     5461 aactattcag ttggtctact aaaagatacc agattagact gacccaaaga accttgtctg
     5521 ccagtgccat gtccttagac caacacccct agcacctgtc cctggccagc tgaccagaag
     5581 gacgcagggc cctgggcaca tctaagcccc agggctgggg ctggcaggga gagttctctg
     5641 gcagctgctg gagaagaagc tcctgcagga gaggaaagaa aggctctgtg acagtttggc
     5701 tgcctgagat gctgctggtg ctactgtgct gcttacttat tttacctgtt gttatttaaa
     5761 gtggtggacc gggctgaggc tgtaggggag gaggagacct cattggggca cactaccgtg
     5821 tcatgtagag gccccagcgc cagtgagggc gcgccttaac acacacacag tgtcacccac
     5881 gtcacgagtt ttgcttgtca gcagcttgag gtgcagtttc ccagaaaccc ctgcacctat
     5941 ctccttgaaa cttggcaggc ttcgtggcct cagcaggggc taccatgcct gctgttttca
     6001 ccccctgtgc cttaacacac acagtgacat gagttttgct tgtcagcact tgaggtgcag
     6061 tttcccagaa acccctacac ctatctcctt gaaacttggc aggcttctgc ctcagaaggg
     6121 gctacctccc tgcgttttca tctaaatcag ccaaaaatga caaagttata ggcagttttg
     6181 tgattcccca ttatagccta tgggcgaaac atcgaaacag cgtcgaaaca gtgaaacagt
     6241 ttttgacgaa atggaacagc gctttgaaac aaatgaaacc aaaatgaaac actgtccctt
     6301 caaaatggcg aaacggaagt cgaaacggaa tggtgctgtt tcgcacagcc ctactatata
     6361 ggggcccaga gacggagtca ctgccaaccg agacggtcat agctgtttcc tgtgtgccgc
     6421 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     6481 ctcaaaggcg gtaatacggt tacccacaga atcaggggat aacgcaggaa agaacatgtg
     6541 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     6601 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     6661 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     6721 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     6781 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     6841 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     6901 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     6961 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     7021 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     7081 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     7141 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     7201 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     7261 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     7321 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     7381 tatctcagcg atctgtctct ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     7441 aactacgata cgggagggct taccatctgg ccccagtgct gcaataatac cgcgggaccc
     7501 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     7561 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     7621 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccatcgcta caggcatcgt
     7681 ggtatcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     7741 agttacatga tcccccatgt tgcgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     7801 tgtcagaagt aagttggccg ccgtgttatc actcatggtt atggcagcac tacataattc
     7861 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     7921 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     7981 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     8041 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     8101 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     8161 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     8221 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     8281 tgaatgtatt tag
//
