LOCUS       sequence-441871-        5123 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor plasmid of MarsTigger1c Transposon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5123)
  AUTHORS   Zhang T, Tan S, Tang N, Li Y, Zhang C, Sun J, Guo Y, Gao H, Cai Y, 
            Sun W, Wang C, Fu L, Ma H, Wu Y, Hu X, Zhang X, Gee P, Yan W, Zhao 
            Y, Chen Q, Guo B, Wang H, Zhang YE
  TITLE     Heterologous survey of 130 DNA transposons in human cells highlights
            their functional divergence and expands the genome engineering 
            toolbox.
  JOURNAL   Cell. 2024 Jul 11;187(14):3741-3760.e30. doi: 
            10.1016/j.cell.2024.05.007. Epub 2024 Jun 5.
  PUBMED    38843831
REFERENCE   2  (bases 1 to 5123)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2024.05.007"; journalName: "Cell"; date: 
            "2024-07-11- 11"; volume: "187"; issue: "14"; pages: "3741-3760.e30"
COMMENT     Sequence Label: pMV-MarsTigger1c
FEATURES             Location/Qualifiers
     source          1..5123
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     94..112
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     121..138
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     122..138
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        713..1212
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(748..771)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     1145..1164
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      1226..1235
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1232..1831
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MGTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     1731..1751
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             1838..1894
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(1895..1897,1898..1900,1901..2614)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1895..1897,1898..1900,1901..2611)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1940..1961)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2201..2220)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2548..2569
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(2636..2653)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2642..2866
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(3226..3242)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3226..3242)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3254..3271)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3425..4013)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3505..3524)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4184..4975,4976..5044))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLRNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERKRQIAEIGAS
                     LIKHW"
     primer_bind     4807..4826
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(5045..5123,1..26))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
       61 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
      121 tgtaaaacga cggccagtcg aaccacgcaa tgcgtctcga tccgcagtgt cttgcgtctc
      181 tcaattgtac aggtatccct tccacatcgc gactttcccc atcgcggttt cgatatatcg
      241 cgggtcggca taagaaatta aatgggaatt ttgggggagt tttgcggaag ccgcagacga
      301 cacgcgaagg ccagcagacg acacagaaaa agtttagaaa ctcagaaatg cataaaatat
      361 atgtatagta ttgtataata tcaacatatt ttatctttta ataccataaa tatacacaat
      421 ttctttttta aagttaaaat aagtaaaaag ttaaagtttg cgaaaagaac gcgaaagcca
      481 gcagatgaca cacaaaggct agaaatgtag agatatcttt aaaaagttta gtaactcata
      541 aatgcataaa atgtatgtac tgttatgtat aatattacct gtacagtatg tattttacta
      601 tttaataaca taaatgaaca gctacacaat ttttttccct taaaattaaa tttgtagtaa
      661 aagaactgcg gttctacgca ctggtttggg gaccggtgct agcaattcta ccgggtaggg
      721 gaggcgcttt tcccaaggca gtctggagca tgcgctttag cagccccgct gggcacttgg
      781 cgctacacaa gtggcctctg gcctcgcaca cattccacat ccaccggtag gcgccaaccg
      841 gctccgttct ttggtggccc cttcgcgcca ccttctactc ctcccctagt caggaagttc
      901 ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact
      961 agtctcgtgc agatggacag caccgctgag caatggaagc gggtaggcct ttggggcagc
     1021 ggccaatagc agctttgctc cttcgctttc tgggctcaga ggctgggaag gggtgggtcc
     1081 gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc
     1141 ccggcattct gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct
     1201 ccgggccttt cgacctgcag ccaacgccac catggggacc gagtacaagc ccacggtgcg
     1261 cctcgccacc cgcgacgacg tcccccgggc cgtacgcacc ctcgccgccg cgttcgccga
     1321 ctaccccgcc acgcgccaca ccgtcgaccc ggaccgccac atcgagcggg tcaccgagct
     1381 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga
     1441 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc
     1501 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat
     1561 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg
     1621 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga
     1681 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc
     1741 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg
     1801 cacctggtgc atgacccgca agcccggtgc catcgatgcc actaacttct ccctgttgaa
     1861 acaagcaggg gatgtcgaag agaatcccgg gccaatggtg agcaagggcg aggagctgtt
     1921 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
     1981 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
     2041 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt
     2101 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
     2161 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
     2221 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
     2281 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
     2341 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg
     2401 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
     2461 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag
     2521 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
     2581 gatcactctc ggcatggacg agctgtacaa gtaattaatt aagctcgctg atcagcctcg
     2641 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     2701 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     2761 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     2821 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcgcgc
     2881 cctcgagagc aagggattac tctttaaggt gttattgctt ctggacaatg cccccggcca
     2941 cccagaaccc catgaattcc atattgaagg cgttgaagtt atctacatgc caactaacac
     3001 aacgtctctc attcagcctc ttgatcaggg ggtaatacgg acatttaagg cacactacac
     3061 acgctattca atggagagga tcatagagac tatcgaagaa aaccccgaca aagacaaaaa
     3121 attttacgcg gattttccag atcgcggggg tgccatgccc ctaacccccg cgatgtggaa
     3181 gggatacctg taggcccaga gacggagtca ctgccaaccg agacggtcat agctgtttcc
     3241 tgtgtgccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     3301 tatcagctca ctcaaaggcg gtaatacggt tacccacaga atcaggggat aacgcaggaa
     3361 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     3421 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     3481 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     3541 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     3601 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     3661 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     3721 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     3781 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     3841 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     3901 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     3961 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     4021 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     4081 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     4141 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     4201 gtgaggcacc tatctcagcg atctgtctct ttcgttcatc catagttgcc tgactccccg
     4261 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaataatac
     4321 cgcgggaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     4381 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     4441 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccatcgcta
     4501 caggcatcgt ggtatcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     4561 gatcaaggcg agttacatga tcccccatgt tgcgcaaaaa agcggttagc tccttcggtc
     4621 ctccgatcgt tgtcagaagt aagttggccg ccgtgttatc actcatggtt atggcagcac
     4681 tacataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     4741 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     4801 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     4861 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     4921 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     4981 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     5041 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     5101 gatacatatt tgaatgtatt tag
//
