LOCUS       sequence-441876-        4667 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor plasmid of Joey1 Transposon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4667)
  AUTHORS   Zhang T, Tan S, Tang N, Li Y, Zhang C, Sun J, Guo Y, Gao H, Cai Y, 
            Sun W, Wang C, Fu L, Ma H, Wu Y, Hu X, Zhang X, Gee P, Yan W, Zhao 
            Y, Chen Q, Guo B, Wang H, Zhang YE
  TITLE     Heterologous survey of 130 DNA transposons in human cells highlights
            their functional divergence and expands the genome engineering 
            toolbox.
  JOURNAL   Cell. 2024 Jul 11;187(14):3741-3760.e30. doi: 
            10.1016/j.cell.2024.05.007. Epub 2024 Jun 5.
  PUBMED    38843831
REFERENCE   2  (bases 1 to 4667)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2024.05.007"; journalName: "Cell"; date: 
            "2024-07-11- 11"; volume: "187"; issue: "14"; pages: "3741-3760.e30"
COMMENT     Sequence Label: pMV-Joey1
FEATURES             Location/Qualifiers
     source          1..4667
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     94..112
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     121..138
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     122..138
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        465..964
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(500..523)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     897..916
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      978..987
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             984..1583
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MGTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     1483..1503
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             1590..1646
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(1647..1649,1650..1652,1653..2366)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1647..1649,1650..1652,1653..2363)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1692..1713)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1953..1972)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     complement(2388..2405)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2394..2618
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(2770..2786)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2770..2786)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2798..2815)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2969..3557)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3049..3068)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3728..4519,4520..4588))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLRNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERKRQIAEIGAS
                     LIKHW"
     primer_bind     4351..4370
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(4589..4667,1..26))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
       61 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
      121 tgtaaaacga cggccagtcg aaccacgcaa tgcgtctcga tccgcagtgt cttgcgtctc
      181 tcaattgtac agtggtgtgc gaattacttg ggacaagcaa taattttgat atggtttcat
      241 tttgtgacag caatggtcat gtgtgtatgc ttctactgac actaggctga ctcagaacgt
      301 ctgaccttga ccagatgaaa ccggattgag ctggtttccc cacctttagt tagttctctg
      361 tcagttgagt gctggatttc gtgccataca catgtgtttc attgctctct tttgttgtgt
      421 ttttaattct gtggttttta agaaccggtg ctagcaattc taccgggtag gggaggcgct
      481 tttcccaagg cagtctggag catgcgcttt agcagccccg ctgggcactt ggcgctacac
      541 aagtggcctc tggcctcgca cacattccac atccaccggt aggcgccaac cggctccgtt
      601 ctttggtggc cccttcgcgc caccttctac tcctccccta gtcaggaagt tcccccccgc
      661 cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca ctagtctcgt
      721 gcagatggac agcaccgctg agcaatggaa gcgggtaggc ctttggggca gcggccaata
      781 gcagctttgc tccttcgctt tctgggctca gaggctggga aggggtgggt ccgggggcgg
      841 gctcaggggc gggctcaggg gcggggcggg cgcccgaagg tcctccggag gcccggcatt
      901 ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct
      961 ttcgacctgc agccaacgcc accatgggga ccgagtacaa gcccacggtg cgcctcgcca
     1021 cccgcgacga cgtcccccgg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg
     1081 ccacgcgcca caccgtcgac ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac
     1141 tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg
     1201 cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg
     1261 gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc
     1321 tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc
     1381 ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg
     1441 agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg
     1501 agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt
     1561 gcatgacccg caagcccggt gccatcgatg ccactaactt ctccctgttg aaacaagcag
     1621 gggatgtcga agagaatccc gggccaatgg tgagcaaggg cgaggagctg ttcaccgggg
     1681 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
     1741 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
     1801 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct
     1861 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
     1921 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
     1981 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
     2041 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
     2101 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
     2161 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     2221 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc
     2281 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     2341 tcggcatgga cgagctgtac aagtaattaa ttaagctcgc tgatcagcct cgactgtgcc
     2401 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     2461 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     2521 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     2581 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgc gccctcgaga
     2641 agttttttaa agatgtaaaa agttgtaagg tttattgtat aagtcaataa atttaattat
     2701 tttactttgt cccagttaat ttgcacacca ctgtagggcc cagagacgga gtcactgcca
     2761 accgagacgg tcatagctgt ttcctgtgtg ccgcttcctc gctcactgac tcgctgcgct
     2821 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttaccca
     2881 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     2941 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     3001 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     3061 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     3121 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     3181 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     3241 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     3301 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     3361 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     3421 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     3481 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     3541 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     3601 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     3661 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     3721 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctctttcgtt
     3781 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     3841 ctggccccag tgctgcaata ataccgcggg acccacgctc accggctcca gatttatcag
     3901 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     3961 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     4021 tgcgcaacgt tgttgccatc gctacaggca tcgtggtatc acgctcgtcg tttggtatgg
     4081 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgcgca
     4141 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgccgtgt
     4201 tatcactcat ggttatggca gcactacata attctcttac tgtcatgcca tccgtaagat
     4261 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     4321 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     4381 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     4441 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     4501 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     4561 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     4621 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttag
//
