LOCUS       sequence-441878-        2193 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  DNA template of GlcR sensor with glcO60 operator: T7 
            promoter-glcO60-3WJdB reporter-T7 terminator linear DNA template 
            (PCR amplified with oSB0021+22) is used in the IVT sensor system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Barthel S, Brenker L, Diehl C, Bohra N, Giaveri S, Paczia N, Erb TJ
  TITLE     In vitro transcription-based biosensing of glycolate for prototyping
            of a complex enzyme cascade.
  JOURNAL   Synth Biol (Oxf). 2024 Sep 20;9(1):ysae013. doi: 
            10.1093/synbio/ysae013. eCollection 2024.
  PUBMED    39399720
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2024-09-20"; volume: "9(1):ysae013. doi"; pages: " 
            10.1093/synbio/ysae013. eCollection 2024"
COMMENT     Sequence Label: pTE5423_p3WJdB-glcO60
FEATURES             Location/Qualifiers
     source          1..2193
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     391..410
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     617..636
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        617..635
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      859..905
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(join(1027..1818,1819..1887))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1650..1669
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1888..1992)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      join(2095..2193,1..490)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
       61 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
      121 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
      181 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
      241 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
      301 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
      361 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
      421 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
      481 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
      541 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgtg cggccgcaac
      601 acgtctcgag taggagtaat acgactcact ataggggtcc agaaaaatac cgattgacta
      661 atgtggtctg aaaattatac caagaatgca gggcagaatg cccacatact ctgatgatcc
      721 gagacggtcg ggtccagata ttcgtatctg tcgagtagag tgtgggctcg gatcattcat
      781 ggcaagagac ggtcgggtcc agatattcgt atctgtcgag tagagtgtgg gctcttgcca
      841 tgtgtatgtg gggcttaata gcataacccc ttggggcctc taaacgggtc ttgaggggtt
      901 ttttgcgctt gttggagacg agctgcggcc gcgattatca aaaaggatct tcacctagat
      961 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     1021 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     1081 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     1141 tggccccagt gctgcaatga taccgcggga cccacgctca ccggctccag atttatcagc
     1201 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     1261 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     1321 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     1381 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     1441 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     1501 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     1561 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     1621 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     1681 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     1741 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     1801 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     1861 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     1921 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     1981 aggggttccg cgcacatttc cccgaaaagt gccacctgtc atgaccaaaa tcccttaacg
     2041 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     2101 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     2161 ggtttgtttg ccggatcaag agctaccaac tct
//
