LOCUS       sequence-441880-        2169 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GlcR module template with glcO36 operator: T7 promoDNA template of 
            GlcR sensor with glcO36 operator: T7 promoter-glcO36-3WJdB 
            reporter-T7 terminator linear DNA tter-glcO36-3WJdB reporter-T7 
            terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Barthel S, Brenker L, Diehl C, Bohra N, Giaveri S, Paczia N, Erb TJ
  TITLE     In vitro transcription-based biosensing of glycolate for prototyping
            of a complex enzyme cascade.
  JOURNAL   Synth Biol (Oxf). 2024 Sep 20;9(1):ysae013. doi: 
            10.1093/synbio/ysae013. eCollection 2024.
  PUBMED    39399720
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2024-09-20"; volume: "9(1):ysae013. doi"; pages: " 
            10.1093/synbio/ysae013. eCollection 2024"
COMMENT     Sequence Label: pTE5419_p3WJdB-glcO36
FEATURES             Location/Qualifiers
     source          1..2169
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     391..410
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     617..636
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        617..635
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      835..881
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(join(1003..1794,1795..1863))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1626..1645
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1864..1968)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      join(2071..2169,1..490)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
       61 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
      121 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
      181 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
      241 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
      301 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
      361 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
      421 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
      481 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
      541 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgtg cggccgcaac
      601 acgtctcgag taggagtaat acgactcact atagggatgt ggtctgaaaa ttataccaag
      661 aatgcagggc agaatgccca catactctga tgatccgaga cggtcgggtc cagatattcg
      721 tatctgtcga gtagagtgtg ggctcggatc attcatggca agagacggtc gggtccagat
      781 attcgtatct gtcgagtaga gtgtgggctc ttgccatgtg tatgtggggc ttaatagcat
      841 aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gcgcttgttg gagacgagct
      901 gcggccgcga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
      961 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     1021 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     1081 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     1141 gcgggaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     1201 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     1261 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     1321 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     1381 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     1441 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     1501 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     1561 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     1621 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     1681 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     1741 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     1801 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     1861 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     1921 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     1981 aaaagtgcca cctgtcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     2041 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     2101 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     2161 accaactct
//
