LOCUS       sequence-441908-        9378 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV.FUS.3 Rep-Cap plasmid for production of AAV.FUS.3, a capsid with
            tropism for CNS neurons upon focused ultrasound mediated delivery..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9378)
  AUTHORS   Li HR, Harb M, Heath JE, Trippett JS, Shapiro MG, Szablowski JO
  TITLE     Engineering viral vectors for acoustically targeted gene delivery.
  JOURNAL   Nat Commun. 2024 Jun 10;15(1):4924. doi: 10.1038/s41467-024-48974-y.
  PUBMED    38858354
REFERENCE   2  (bases 1 to 9378)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-06-10"; volume: "15(1):4924. doi"; pages: " 
            10.1038/s41467-024-48974-y"
COMMENT     Sequence Label: CAP-AAV.FUS.3
FEATURES             Location/Qualifiers
     source          1..9378
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             complement(1482..1493)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    4210..4228
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4247..4265
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4284..4302
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4321..4339
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4358..4376
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4395..4413
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4432..4450
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4469..4487
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     polyA_signal    4549..4773
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     4762..4779
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     promoter        4799..4817
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     4799..4816
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             complement(4856..4966)
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
     primer_bind     complement(7530..7546)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7531..7547)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(7575..7594)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7576..7594)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7604..7621)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7604..7620)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7613..7635)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7762..8217
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7849..7868)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8059..8080
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8243..8347
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8348..8416,8417..9208)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8566..8585)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctcca ccgcggtggc ggccgctcta gtcgagcggc
     1081 cgccagtgtg atggatatct gcagaattcg gcttggcggc tgcgcgttca aacctcccgc
     1141 ttcaaaatgg agaccctgcg tgctcactcg ggcttaaata cccagcgtga ccacatggtg
     1201 tcgcaaaatg tcgcaaaaca ctcacgtgac ctctaataca ggacctctag tcctgcaggt
     1261 ttaaacgaat tcgcccttcg cagagaccaa agttcaactg aaacgaatta aacggtttat
     1321 tgattaacaa gcaattacag attacgagtc aggtatctgg tgccaatggg gcggggttca
     1381 ctatatacac cttcagtatt aacagcaaat tcaacattat tagacttgta atagttggaa
     1441 gtgtactgga tctccgggtt ccagcgcttg ctgttttcct tctgcagctc ccactcgatc
     1501 tccacgctga cttggccagt agaatactgg gtgatgaaag agttcagctt gtccttgttg
     1561 aaggccgttg gaggatccgc aggtacaggt gtgtttttga tgaggatctg aggaggcggg
     1621 tgcttcattc caaaccctcc catcagcgga gaagggtgaa agttgccgtc cgtgtgagga
     1681 attttggccc aaatgggtcc ttgcaggtac acatctctgt cctgccaaac catacccgga
     1741 agtattcctt ggttttgaac ccaaccggtc tgcgcctgtg cgggctggcc gccctcgctc
     1801 cattgggcac tctggtggtt tgtggccact tgtccatagg actccgttgc taccgggtta
     1861 gtagttttaa tttcttcttc gttggttatc atgactttgt ccgcatccac gttgtctctt
     1921 ccagttcctt gtttgccaaa aattaaagat ccagacaaag gaaagaaacg gtcctctcct
     1981 tctttgtggc tggccatagc aggtccagga ttcatcaagc tattacgtcc attgagagcc
     2041 caagaagaag ctccaggcca agcaaattcg ctgttgttgt tttgagtcac agtggttgag
     2101 acacgttgtt gtcggtagct gggtccaggt atgtagtttc ttccctggac agccatgttg
     2161 ctgggtccgg ccacactgaa ttttagcgtt tgttgattct gtccagaacc gttaatagtt
     2221 ctagagagat agtacaagta ttggtcgatg agtggattca ttagtcggtc caggctttgg
     2281 ctgtgagcgt agctgctatg gaaaggtacg ttctcaaact cgtagctgaa ctggaagttg
     2341 ttacccgttc ttagcatttg cgacgggaaa tattccaggc agtaaaagga cgaacgaccc
     2401 acggcctggc ttccatcatt aagcgtcaga tacccgtact gaggaatcat gaaaacgtcc
     2461 gctgggaacg gcgggaggca gccctcgtga gccgacccga gcacgtacgg gagctgatag
     2521 tctgagtccg tgaagacctg gaccgtgctg gtaaggttat tggcgatggt cttgactcca
     2581 ttgttgtccg taacctcttt gacctgaatg ttgaagagct tgaagttgag tcgcttaggc
     2641 cggaatcccc agttgttgtt gatgagtcgc tgccagtcac gtggtgagaa gtggcagtgg
     2701 aatctgttga agtcaaaata cccccagggg gtgctgtagc cgaagtaggc gttgtcattt
     2761 gaagatcctc cagatgtgct gttggagatt tgcttgtaga ggtgattgtt gtaggtgggc
     2821 agggcccagg ttcgggtgct ggtggtgatg actctgtccc ccagccattg ggaatcgcaa
     2881 tgccaatttc ccgaggaact acccactcca tcggcacctt cgttattgtc tgccactggt
     2941 gcgccaccac ctgaagccat tgtaagagat cccacacctg agggggctgc gggaggttct
     3001 ccgattggtt gagggtctgg gactgactct gtgtcgccag tctgaccgaa attgagtctc
     3061 tttttagcgg gctgtgcacc cgatttgcca atacccgcgg aggagtccgg ttcctgagga
     3121 gactgctcta caggcctctt ctttccagga gccgtcttag ccgcttcctc aaccagacca
     3181 agaggttcaa gaagcctctt tttggcctgg aagactgctc gcccgaggtt gcccccaaaa
     3241 gacgtatctt ctttgagccg ctcctggaac tcggcgtcgg cgtggttgta cttgaggtac
     3301 gggttgtctc cggccttgag ctgctggtcg taggccttgt cgtgctcgag ggccgccgcg
     3361 tctgctgcgt tgaccggctc ccccttgtcg agtccgttgc cgggtccaag gtatttgtaa
     3421 cccggaagca caagacctcg agcgttgtct tgatgttgtt gatttgcctt gggttgaggg
     3481 gctccaggtt tcaaagccca ccactcgcga attccttcac taaggttgtc ctcgagccaa
     3541 tctggaagat aaccatcggc agccatacct ggtttaagtc atttattgtt cagaaacaca
     3601 gtcatccaag tccacattga ccaggtcgca agcagtgcaa gcgtctggca cctttcccat
     3661 gatgtgatga atgtagcaca gtttctgata cgcctttttg acgacagaaa cgggttgaga
     3721 ttctgacacg ggaaagcact ctaaacagtc tttgacaccg tgagtgaagc agatatttga
     3781 attctgattc agtctctcgc attgtctgca gggaaacagc atcagattca tgcccacgtg
     3841 acgagaacat ttgttttggt acctgtccgc gtagttgatc gaagctagcg gtcgcagagg
     3901 agcgggatca acagtcacgt ctgaactgcg aggcgtctcg ggaacaaatg agagcctggc
     3961 ccgcttctcc agacttttat agctagtatt ggtgacgtca cccagtgggc gttttagaga
     4021 tttctccgcc cctttagttc ccgccaattc cctgggaact ttaaactcgt gagtcaccgg
     4081 cacctgattg acctttgccc aagcaaaaaa gtccttgact tcctgcttag taatcttgcc
     4141 aaaatctggc gggagccgct tagtcagttc aaatttgaac agatctctct gcttatatag
     4201 ggggtacctt ctctatcact gatagggagc tcgagacgtg tcgacttctc tatcactgat
     4261 agggagacgt tctagacgta ccttctctat cactgatagg gagctcgaga cgtgtcgact
     4321 tctctatcac tgatagggag acgttctaga cgtaccttct ctatcactga tagggagctc
     4381 gagacgtgtc gacttctcta tcactgatag ggagacgttc tagacgtacc ttctctatca
     4441 ctgataggga gctcgagacg tgtcgacttc tctatcactg atagggagac gtcaggtggc
     4501 acttgatagc ttgattcgag ccccagctgg ttctttccgc ctcagaagcc atagagccca
     4561 ccgcatcccc agcatgcctg ctattgtctt cccaatcctc ccccttgctg tcctgcccca
     4621 ccccaccccc cagaatagaa tgacacctac tcagacaatg cgatgcaatt tcctcatttt
     4681 attaggaaag gacagtggga gtggcacctt ccagggtcaa ggaaggcacg ggggaggggc
     4741 aaacaacaga tggctggcaa ctagaaggca cagtcgaggc tgatcagcga gctctagcat
     4801 ttaggtgaca ctatagaata gggccctcta gatcgaattc atgcatttac ccggggagca
     4861 tgtcaaggtc aaaatcgtca agagcgtcag caggcagcat atcaaggtca aagtcgtcaa
     4921 gggcatcggc tgggagcatg tctaagtcaa aatcgtcaag ggcgtcggcc ggcccgccgc
     4981 tttcgcactt tagctgtttc tccaggccac atatgattag ttccaggccg aaaaggaagg
     5041 caggttcggc tccctgccgg tcgaacagct caattgcttg tctcagaagt gggggcatag
     5101 aatcggtggt aggtgtctct ctttcctctt ttgctacttg atgctcctgt tcctccaata
     5161 cgcagcccag tgtaaagtgg cccacggcgg acagagcgta cagtgcgttc tccagggaga
     5221 agccttgctg acacaggaac gcgagctgat tttccagggt ttcgtactgt ttctctgttg
     5281 ggcgggtgcc gagatgcact ttagccccgt cgcgatgtga gaggagagca cagcggaatg
     5341 acttggcgtt gttccgcaga aagtcttgcc atgactcgcc ttccaggggg cagaagtggg
     5401 tatgatgcct gtccagcatc tcgatggcca gggcatcgag cagggcccgc ttgttcttca
     5461 cgtgccagta cagggtaggc tgctcaactc ccagcttttg agcgagtttc cttgtcgtca
     5521 ggccttcgat accgacttca ttgagtaatt ccagagcgcc gtttatgact ttagatttat
     5581 ccagccgact catacctggt ttaagtcatt tattgttcag aaacacagtc atccaagtcc
     5641 acattgacca ggtcgcaagc agtgcaagcg tctggcacct ttcccatgat gtgatgaatg
     5701 tagcacagtt tctgatacgc ctttttgacg acagaaacgg gttgagattc tgacacggga
     5761 aagcactcta aacagtcttt gacaccgtga gtgaagcaga tatttgaatt ctgattcagt
     5821 ctctcgcatt gtctgcaggg aaacagcatc agattcatgc ccacgtgacg agaacatttg
     5881 ttttggtacc tgtccgcgta gttgatcgaa gcttccgcgt ctgacgtcga tggctgcgca
     5941 actgactcgc gcacccgttt gggctcactt atatctgcgt cactgggggc gggtcttttc
     6001 ttggctccac cctttttgac gtagaattca tgctccacct caaccacgtg atcctttgcc
     6061 caccggaaaa agtctttgac ttcctgcttg gtgaccttcc caaagtcatg atccagacgg
     6121 cgggtgagtt caaatttgaa catccggtct tgcaacggct gctggtgttc gaaggtcgtt
     6181 gagttcccgt caatcacggc gcacatgttg gtgttggagg tgacgatcac gggagtcggg
     6241 tctatctggg ccgaggactt gcatttctgg tccacgcgca ccttgcttcc tccgagaatg
     6301 gctttggccg actccacgac cttggcggtc atcttcccct cctcccacca gatcaccatc
     6361 ttgtcgacac agtcgttgaa gggaaagttc tcattggtcc agtttacgca cccgtagaag
     6421 ggcacagtgt gggctatggc ctccgcgatg ttggtcttcc cggtagttgc aggcccaaac
     6481 agccagatgg tgttcctctt gccgaacttt ttcgtggccc atcccagaaa gacggaagcc
     6541 gcatattggg gatcgtaccc gtttagttcc aaaattttat aaatccgatt gctggaaatg
     6601 tcctccacgg gctgctggcc caccaggtag tcgggggcgg ttttagtcag gctcataatc
     6661 tttcccgcat tgtccaaggc agccttgatt tgggaccgcg agttggaggc cgcattgaag
     6721 gagatgtatg aggcctggtc ctcctggatc cactgcttct ccgaggtaat ccccttgtcc
     6781 acgagccacc cgaccagctc catgtacctg gctgaagttt ttgatctgat caccggcgca
     6841 tcagaattgg gattctgatt ctctttgttc tgctcctgcg tctgcgacac gtgcgtcaga
     6901 tgctgcgcca ccaaccgttt acgctccgtg agattcaaac aggcgcttaa atactgttcc
     6961 atattagtcc acgcccactg gagctcaggc tgggttttgg ggagcaagta attggggatg
     7021 tagcactcat ccaccacctt gttcccgcct ccggcgccat ttctggtctt tgtgaccgcg
     7081 aaccagtttg gcaaagtcgg ctcgatcccg cggtaaattc tctgaatcag tttttcgcga
     7141 atctgactca ggaaacgtcc caaaaccatg gatttcaccc cggtggtttc cacgagcacg
     7201 tgcatgtgga agtagctctc tcccttctca aattgcacaa agaaaagagc ctccggggcc
     7261 ttactcacac ggcgccattc cgtcagaaag tcgcgctgca gcttctcggc cacggtcagg
     7321 ggtgcctgct caatcagatt cagatccatg tcagaatctg gcggcaactc ccattccttc
     7381 tcggccaccc agttcacaaa gctgtcagaa atgccgggca gatgctcgtc aaggtcgctg
     7441 gggaccttaa tcacaatctc gtaaaacccc ggcatggcgg ctgcgcgttc aaacctcccg
     7501 cttcaaaatg gagaccctgc gagctccccc gataccgtcg acctcgatcg agggggggcc
     7561 cggtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac
     7621 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     7681 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     7741 gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     7801 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     7861 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     7921 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     7981 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     8041 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     8101 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     8161 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg
     8221 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     8281 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     8341 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     8401 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     8461 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     8521 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     8581 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     8641 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     8701 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     8761 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     8821 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     8881 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     8941 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     9001 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     9061 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     9121 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     9181 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     9241 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     9301 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     9361 aaagatcaaa ggatcttc
//
