LOCUS       sequence-442020-        8039 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression vector for expression of hAT-10_XT transposase 
            (CMV promoter).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8039)
  AUTHORS   Zhang T, Tan S, Tang N, Li Y, Zhang C, Sun J, Guo Y, Gao H, Cai Y, 
            Sun W, Wang C, Fu L, Ma H, Wu Y, Hu X, Zhang X, Gee P, Yan W, Zhao 
            Y, Chen Q, Guo B, Wang H, Zhang YE
  TITLE     Heterologous survey of 130 DNA transposons in human cells highlights
            their functional divergence and expands the genome engineering 
            toolbox.
  JOURNAL   Cell. 2024 Jul 11;187(14):3741-3760.e30. doi: 
            10.1016/j.cell.2024.05.007. Epub 2024 Jun 5.
  PUBMED    38843831
REFERENCE   2  (bases 1 to 8039)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2024.05.007"; journalName: "Cell"; date: 
            "2024-07-11- 11"; volume: "187"; issue: "14"; pages: "3741-3760.e30"
COMMENT     Sequence Label: pCMV-hAT-10_XT
FEATURES             Location/Qualifiers
     source          1..8039
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      2355..2364
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(4227..4245)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4228..4245)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4265..4282)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4271..4495
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4541..4969
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4628..4647)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4838..4859
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4978..4998)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4983..5312
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5163..5298
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5225..5244
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    5478..5599
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5515..5534)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5569..5588
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5648..5664)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5648..5664)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5661..5683)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5672..5688
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5696..5702,5703..5720,5721..5726))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5741..5762
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5879..5896)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6050..6638)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6130..6149)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6809..7600,7601..7669))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7432..7451
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7670..7774)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7849..7868)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctt
      661 ggtaccgagc tcggatccac tagtaacggc cgccagtgtg ctggaattcg ccgccaccat
      721 gactggtcgt ggtcgtggtc gtaaaggtgg tatgggtggc aaaggcggtg aaggcggtcg
      781 taaaggcggt gttggtggtg aaggtggccg caaaggtggt gttggtggtg aaggtggcgt
      841 tggcggtaaa ggtggtttag gcggtcgttc acgtggtgca ggttgtggtc aggaacctag
      901 cactcgtgcg atgcctagct taggcaacat aggccagcag ccgcaacaac agagccaaca
      961 gcagcagcaa cagttacagc agcagcaggt tgcaggtcgt tcaggtgcga ccggtagccg
     1021 taaaaccctg cctgactttt ttgccaccac cagccgccct atacaaagcc aacaggcgga
     1081 agcggttatg gattggttaa cccaggcaac tagcagcgca gcggatagca gcgaacaaca
     1141 agaggtggca agccctgcat caagcgtgat tcgcggtatg ctgagccctc tgctgtttga
     1201 tccggaggtg gacttttcac cggacaccca ggatctgttt gatgaccaga ccggcatggg
     1261 tggtgcattt ctgagcgacg aagaagaggt gtatgtggcc cagcatacca ccagcgcagt
     1321 tggtgatgtg ggccaaggtc ctttaatggc cggtcagcag gcggatgaac ctgatgtgga
     1381 cgtggacgca ggtagcgatt taggcgagga cgtggaagat gatggcgatc gcagctgggt
     1441 tcctgcccaa gaagacaaca gcagcagcag cgaaggcgag ttatgcgttg ttgtgagcga
     1501 cgccgatgaa gaaccggcgc cgaaaaaaca gaaaacccct ccgaccacag cacgtggtgg
     1561 tcgtcaacaa ggtagcaccg cacctggttc aaccggttca ggtcgtatgg gtcgtgatct
     1621 gcaacctagc ggtcctcaac ctagcaccgc aggcaaagtt gttgcgcgta cctcagcagt
     1681 ttgggcgttc tttacctgcc agagccagga tcctagcgtg gcggtttgtc atctgtgccg
     1741 ccaaaaagtt cgtcgtggcc agaccggtag ccatatgggt actagcgcgc tgagcagcca
     1801 tatgaaacgc caccatcgca tgacctggga acaacaccaa agcggccgtg cacctggtgg
     1861 ttcaggtgca tcatgtccgc ctcctcctat tcctcaaggt accggtgcca gctcacctat
     1921 acctgtggcg cgcgaagaag atagcgaggc gcattcatgg cgtcgtccgt tatgtagctc
     1981 agcgagctca gcagcacctt cagtgagcag cagcagctta ttaccgagcg cacagccttt
     2041 aagccgtcag gtgagcctgc cgcaattctt aggtcgcaaa gcccctttaa gcagcagcca
     2101 tcctcaggtg caacgtttaa acgcctgcct ggcaaagtta ctggcggttc agttactgcc
     2161 gtatcagctg gttgatgcgg cgccttttcg tcaactgatg gcatgtgcgg caccgaattg
     2221 gcgtatacct agccgccatt attttgcgcg caaggccgtg cctgcattac atcagcaggt
     2281 ggtggataac gtgagcctga gcctggacta tgcagttggt ggccgcgttc attgtaccac
     2341 cgatacctgg actagccgcc atggtcaggg tcgctatata agctataccg cgcactgggt
     2401 gactttaatg agcgcaggtg aaggtgcagg tagccgtact cctttacgtc tggaagtgcc
     2461 tcctcgtggc gttaagggta aaccgcatac cgcaaccagc agcagctcaa gcagcaccat
     2521 ggatgagcct ccgttaaaac gccctcgcag ctatgcgagc gtgcagtata aacgctgcca
     2581 ggccgtgtta cagttactgt gcctgggtga acgtagccat accgcgcctg aattacacgc
     2641 agcctttcag acccaagtgc agcgttggtt taccccgcgc cagttacaag caggcaacgt
     2701 ggtttgtgat aacggccgca atctgttagc ggcgctgcat ctgggtcgct taacccatgt
     2761 tccgtgcttt gcccatgtgc tgaacctggt ggtgcagcat tttctgaaga gctacaccgg
     2821 cttaggcgtg gttctggaaa aagcgcgtcg tgtgtgcggt cattttcgtc gcagccctac
     2881 tgcgtcagca agcttaagcc gcatgcaacg tcagcatagc ttacctccgc accgcctgat
     2941 atgcgattta ccgacccgct ggaacagcac cctgcatatg gtggagcgcc tggttgaaca
     3001 acgttgggcg gtgagcaact atctgctgga acatagcgca cgtggtaccc agggtgcgaa
     3061 cttaggctac ttcagcaccg aacagtggca gcaaatgcgt caactgtgcc gcgttttagc
     3121 gccttttgag caggcgaccc gctttgtgag ccgtgataac gcatgcgtga gcgacattat
     3181 ccctctggtg ttcctgctga aacgcaccct ggatggcctg ttagaagaag gcgacgttcc
     3241 tgaggaagag gaatgtcgtc cgcgtcgcca agttgaaggt gcagcgcgcc atgacgaaga
     3301 agacatgccg aacgaagaag atgaaggcga ggaggattgg gttcctgccg aacagggcga
     3361 acaaggtcct cgtcatccta ccacccctgc agttgtgcgt ggttgggaag ataccgaaca
     3421 aggtgaagag gcgggcgacg atctgttaca tctgcagggc agccaggaag aagttggtcg
     3481 cggccatctg ttttatatgg ccgcccacat gcaaacctgc ttacgcagcg atagccgcat
     3541 ttgcgccatt aaggatcgcg aagattattg ggtggcgacc atgattgatc cgcgctacaa
     3601 agaaaaaatg gcgcagtttc tggttcctag ccagcgtgaa cgtcgtgttg gccaactgaa
     3661 acgtgcgtta tgcgcgaaac tgatggaagc cttcccgcaa cctgataccc aagcgagcac
     3721 cacccctcat attcagcagc gccaagagtc atcaagcagc agctcatcag cagcgcgtgg
     3781 tggtggtaac ctgatggatg tgtggcgcag cttttttgag cctcgtcaag cagcggcggg
     3841 tttagttagc acccagagcc atcatcagca gcgcctggat aacatggtgg ccgattacat
     3901 gggtagcgtg agcgttcagg acgcgatgca tactgatgac gacccgatga acttttgggt
     3961 gagccgctta gatcagtggc cggaattagc ccagtatgcg ttagaagtgc tggcatgccc
     4021 tcctgcatca gttctgagcg agcgcgtttt tagcgcagca ggcggtgtgg ttaccgaaaa
     4081 acgcacccgt ctgagcactg gttcagtgga tcgcctgagc tttatcaaga tgaacgaagc
     4141 gtggattagc ggcgacattc atgtgccgat tgccgatatt cgcgattgat gagcggccgc
     4201 tcgagcatgc atctagaggg ccctattcta tagtgtcacc taaatgctag agctcgctga
     4261 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     4321 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     4381 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     4441 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct
     4501 gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca
     4561 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     4621 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     4681 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     4741 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     4801 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     4861 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     4921 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga
     4981 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     5041 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     5101 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     5161 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     5221 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag
     5281 gaggcttttt tggaggccta ggcttttgca aaaagctccc cgaaatgacc gaccaagcga
     5341 cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct
     5401 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg
     5461 agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     5521 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     5581 aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt
     5641 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     5701 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     5761 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     5821 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     5881 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     5941 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     6001 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     6061 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     6121 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     6181 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     6241 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     6301 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     6361 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     6421 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     6481 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     6541 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     6601 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     6661 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     6721 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     6781 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     6841 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     6901 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     6961 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     7021 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     7081 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     7141 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     7201 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     7261 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     7321 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     7381 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     7441 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     7501 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     7561 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     7621 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     7681 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     7741 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     7801 acgtcgacgg atcgggagat ctcccgatcc cctatggtgc actctcagta caatctgctc
     7861 tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta
     7921 gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa
     7981 tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgtt
//
