LOCUS       sequence-442021-        7130 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression vector for expression of Arthur1 transposase 
            (CMV promoter).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7130)
  AUTHORS   Zhang T, Tan S, Tang N, Li Y, Zhang C, Sun J, Guo Y, Gao H, Cai Y, 
            Sun W, Wang C, Fu L, Ma H, Wu Y, Hu X, Zhang X, Gee P, Yan W, Zhao 
            Y, Chen Q, Guo B, Wang H, Zhang YE
  TITLE     Heterologous survey of 130 DNA transposons in human cells highlights
            their functional divergence and expands the genome engineering 
            toolbox.
  JOURNAL   Cell. 2024 Jul 11;187(14):3741-3760.e30. doi: 
            10.1016/j.cell.2024.05.007. Epub 2024 Jun 5.
  PUBMED    38843831
REFERENCE   2  (bases 1 to 7130)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2024.05.007"; journalName: "Cell"; date: 
            "2024-07-11- 11"; volume: "187"; issue: "14"; pages: "3741-3760.e30"
COMMENT     Sequence Label: pCMV-Arthur1
FEATURES             Location/Qualifiers
     source          1..7130
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(3318..3336)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3319..3336)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3356..3373)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3362..3586
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3632..4060
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3719..3738)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3929..3950
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4069..4089)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4074..4403
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4254..4389
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4316..4335
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    4569..4690
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4606..4625)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4660..4679
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4739..4755)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4739..4755)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4752..4774)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4763..4779
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4787..4793,4794..4811,4812..4817))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4832..4853
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4970..4987)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5141..5729)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5221..5240)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5900..6691,6692..6760))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6523..6542
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6761..6865)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6940..6959)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctt
      661 ggtaccgagc tcggatccac tagtaacggc cgccagtgtg ctggaattcg ccgccaccat
      721 gatgggccgc aaatacccta gcggcagcca gaaacgcaag ctgaaagagc gccgtttagc
      781 agaagcgagc aagtgccaga aactgagcta ttataacacc accgaaacca aaaacagcga
      841 tggccagaac cacgaaaaca agaagaaaaa ccacgacagc caggaaaaca ttgacaacaa
      901 aaacgacaac cgccgcaaca aggaggatta cgaaaaccag gataccctga agcaggaaac
      961 caaaaagcag aaagaagacg aaagcaacag caaagagaag cgcaacctgt atggccagca
     1021 gagcacccat gaaggtcagc agcagctgca ttttgatgaa tttgtggact tcgataaata
     1081 ccgcgatctg agcttatggc ctgtgaccat gagcaacaac tttattcagc attgcctgat
     1141 gaaagaagtg tgcttcttca agatcgtgat cccgatcatc tttaccgaaa accagaccga
     1201 acacaccatg gataaacgcg atgtgagcca gaccgatatt ctgaagaaga ttaagaacgg
     1261 ccagaccgtg atgcgtagct ggctggcgta tagcaaaacc aaaggctgca tctattgctt
     1321 tgtgtgcaag ctgttcagca cccgtcagac cttactgcgt agcgacgagt tcagcgattg
     1381 gatgaacatt ctgcgcaccc tgaaaagcca tgaagatagc accgaacata agaaagccat
     1441 gtttacctgg atcacccgca aaagcaacaa aaacgccctg gatcagcgct tagaagagca
     1501 gcgcaagaac attcgctatt acttcgaagt gctgaagcgt gtggtggcgg tgattaaatt
     1561 cctgagcgaa cgcggtctgc cttttcgtgg ccatgatgag aaatggcgca gcagcaacaa
     1621 cggcaacttt atgggcatca tcaaactgat tgcggagttt gatccgtttc tgcatgagca
     1681 tctggaaaaa tgccagaacg aaaaaaccaa cgtgacctat ctgagcaaaa ccgtgtatga
     1741 ggagctgatt gagattatgg gcaaacgcgt gcagaacgaa gtggtgaacc agattaacaa
     1801 accggatacc aagtactaca gcattatcgt ggatagcacc cctgatgtga ccaacaccga
     1861 tcagctggcg atcattgtgc gctattgcta caacggcaaa ccgtatgaac gctttctgac
     1921 ctttagcccg accgaaaacc atctgagcgt gaccctgttt aacaaagtga agcaggtgct
     1981 ggatgactgt aacctgccgc tgaacaacat tcgtggccgc agctatgata atgcggcggt
     2041 gatgaagggc gaagataagg gtctgcaggc gctgtttaag aacattaaca agtacgcgga
     2101 gtacgttcct tgtgcggcgc atagcttaaa tctggtgggc gaaaaagcgg caagcaccgt
     2161 tcctgaagtg gtggattatt ttggcatcct gcagcagctg tatgtgttct ttagcggctc
     2221 aagccgccgt tggagcattt taaacaccca cgccaacctg gacttcagcc tgaaaagcct
     2281 gagcgttacc cgttggagcg cccattataa agcggtgcag gccctgcaac atggctataa
     2341 cgatatcctg cgcaccctga agtacatttt tgaggacagc gaggagaagc ctgaacgcaa
     2401 acgcgatgcc aagagcctgt ttaagaaact gatcaagctg gagtatgcca ttctgaccgc
     2461 catttggaaa gatgtgctgg aacgctttaa caaaaccagc gaaaaactgc agacccctga
     2521 tctggatgtg tatgaaggct acctgctgct gtcaagcctg aacctgttta tcaaagaact
     2581 gcgcgaaaac agcgataaaa agctgatgga gtacgaaacc aaggcgatca aaatgagcaa
     2641 cgagattaac cgcaactaca gcgatattga gaaacagatc gtgaccaaga agtttagcga
     2701 ccataccaaa agcagcaaca gtctgcgtgg ccgcgataag tttcgcattg aagtgatgaa
     2761 ccgcctgctg gattgcctga tcattcagct gatgaaacgc agcgagagct acgaacatat
     2821 tggcaagcgc tttaagtttc tggccgatct gacccgcaac accgatattg acgaggataa
     2881 catcaaactg attatccgcc actacaacga agatatcgat gacaaactgg tgaacgaatg
     2941 ccgccagttc aaagaatatc tgcgcctggt gaccgcacag gaaaacctga aatgcccgga
     3001 aatcctgcag ctgatttatg agcgcaacct gatcgaagtg tttccgaacc tgaccaccat
     3061 cctgaaaatc tatatgaccc tgccgatcac cagctgtgaa gcggaacgca actttagcaa
     3121 actgagcatt atcaagaaca agtttcgcag caccatgctg gaagaacgcc tgaactacct
     3181 gagcattctg agcattgaaa acgacattac caagagcctg agctatgaag aagcgatcaa
     3241 agaatatgcg gcgaagaagt gccgcaaaaa ggtgctgtaa tgagcggccg ctcgagcatg
     3301 catctagagg gccctattct atagtgtcac ctaaatgcta gagctcgctg atcagcctcg
     3361 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     3421 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     3481 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     3541 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     3601 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg
     3661 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     3721 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     3781 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     3841 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     3901 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     3961 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     4021 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc
     4081 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     4141 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     4201 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     4261 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     4321 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt
     4381 ttggaggcct aggcttttgc aaaaagctcc ccgaaatgac cgaccaagcg acgcccaacc
     4441 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
     4501 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
     4561 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     4621 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     4681 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt
     4741 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     4801 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     4861 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     4921 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     4981 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     5041 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     5101 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     5161 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     5221 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     5281 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     5341 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     5401 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     5461 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     5521 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     5581 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     5641 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     5701 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     5761 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     5821 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     5881 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     5941 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     6001 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     6061 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     6121 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     6181 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     6241 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     6301 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     6361 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     6421 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     6481 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     6541 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     6601 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     6661 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     6721 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     6781 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     6841 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg
     6901 gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct ctgatgccgc
     6961 atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag
     7021 caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag
     7081 ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt
//
