LOCUS       sequence-442024-        5582 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression vector for expression of Tc1-3_ST transposase 
            (CMV promoter).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5582)
  AUTHORS   Zhang T, Tan S, Tang N, Li Y, Zhang C, Sun J, Guo Y, Gao H, Cai Y, 
            Sun W, Wang C, Fu L, Ma H, Wu Y, Hu X, Zhang X, Gee P, Yan W, Zhao 
            Y, Chen Q, Guo B, Wang H, Zhang YE
  TITLE     Heterologous survey of 130 DNA transposons in human cells highlights
            their functional divergence and expands the genome engineering 
            toolbox.
  JOURNAL   Cell. 2024 Jul 11;187(14):3741-3760.e30. doi: 
            10.1016/j.cell.2024.05.007. Epub 2024 Jun 5.
  PUBMED    38843831
REFERENCE   2  (bases 1 to 5582)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2024.05.007"; journalName: "Cell"; date: 
            "2024-07-11- 11"; volume: "187"; issue: "14"; pages: "3741-3760.e30"
COMMENT     Sequence Label: pCMV-Tc1-3_ST
FEATURES             Location/Qualifiers
     source          1..5582
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(1770..1788)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1771..1788)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1808..1825)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1814..2038
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2084..2512
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2171..2190)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2381..2402
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2521..2541)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2526..2855
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2706..2841
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2768..2787
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    3021..3142
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3058..3077)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3112..3131
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3191..3207)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3191..3207)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3204..3226)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3215..3231
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3239..3245,3246..3263,3264..3269))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3284..3305
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3422..3439)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3593..4181)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3673..3692)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4352..5143,5144..5212))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4975..4994
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5213..5317)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5392..5411)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctt
      661 ggtaccgagc tcggatccac tagtaacggc cgccagtgtg ctggaattcg ccgccaccat
      721 gcctcagatg agccaggtgt tacgtgaacg cgcgataggt atgttaaccg cgggtatgag
      781 cactcgtgcg gttgcacgtg aactgaatgt gcacttcagc accataagcc gcttacagcg
      841 ccgttttcgc gaatttggca gcactagcaa ccgtccgcat aatcgtcgtc ctcgcgttac
      901 cacccctgca caggacctgc atattcagca cctgcacctg caagatcgct tacgtcctgc
      961 aacccgtact gcggcagcaa ccattggtct gcacaaccag cgcattagcg cgcaaaccgt
     1021 tcgtaaccgc ttacgcgagg cgcatctgca tgcacgtcgt cctcatcgtg gcttagatct
     1081 gaccgctgtg cgtcgtcgca atcgtttaga gtgggccaat gcccatattc gttggcgcct
     1141 ggcgttatgg cgtggcgtgc tgtttaccga cgaaagccgc tttagcctgt atcgcgcgga
     1201 tggtcgtcag cgtgtttggc gccgtgttgg tgaacgcttt gccgacgtga atgtggttga
     1261 ccgcgttgca catggtggtg gtggtgttat ggtttgggcg ggcgtttgct atggtcaacg
     1321 cacccaggtg cattttatcg atggcatcct gaacgcgcaa cgctaccgtg atgaaatcct
     1381 gcgccctatt gtggtgccgt tcattcacga tcaccacctg atgctgcagc atgacaacgc
     1441 gcgtcctcac gttgcgcgca tttgcaccca atttctggag gccgagaaca tacctgttct
     1501 ggcgtggcct gcatactcac ctgacatgag cccgattgaa cacgtgtggg atgccttaga
     1561 tcgccgcatt cgtcaacgtg ttccggttcc tgccaacatc caacaactgc gcaccgcaat
     1621 tgaggaagag tggaccaaca tccctcaggc gaccatcaac aacctgatca acagcatgcg
     1681 ccgccgttgt gttgcattac gcgaagcgaa tggcggtcac acccgctatt gatgagcggc
     1741 cgctcgagca tgcatctaga gggccctatt ctatagtgtc acctaaatgc tagagctcgc
     1801 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     1861 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     1921 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     1981 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct
     2041 tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc
     2101 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     2161 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     2221 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     2281 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     2341 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     2401 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     2461 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt
     2521 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     2581 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
     2641 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
     2701 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     2761 ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt
     2821 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct ccccgaaatg accgaccaag
     2881 cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg
     2941 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
     3001 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca
     3061 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     3121 ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg
     3181 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     3241 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     3301 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     3361 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     3421 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     3481 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     3541 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     3601 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     3661 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     3721 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     3781 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     3841 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     3901 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     3961 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     4021 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     4081 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     4141 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     4201 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     4261 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     4321 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     4381 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     4441 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     4501 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     4561 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     4621 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     4681 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     4741 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     4801 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     4861 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     4921 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     4981 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     5041 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     5101 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     5161 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     5221 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     5281 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     5341 ctgacgtcga cggatcggga gatctcccga tcccctatgg tgcactctca gtacaatctg
     5401 ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga
     5461 gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa
     5521 gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg
     5581 tt
//
