LOCUS       sequence-442028-       10710 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express Tasmanian devil PDL1 protein 
            (saha_PDL1_FL_DN11026_c0_g1_i1).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10710)
  AUTHORS   Slyp B, Darby JM, Flies AS
  TITLE     Conversion of Mouse-Derived Hybridomas to Tasmanian Devil 
            Recombinant IgG Antibodies.
  JOURNAL   Methods Mol Biol. 2024;2826:231-249. doi: 
            10.1007/978-1-0716-3950-4_17.
  PUBMED    39017897
REFERENCE   2  (bases 1 to 10710)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2024"; volume: "2826:231-249. doi"; pages: " 
            10.1007/978-1-0716-3950-4_17"
COMMENT     Sequence Label: pAF146
FEATURES             Location/Qualifiers
     source          1..10710
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     1134..1153
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1152..1207
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1253..1272
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(1372..2397)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(1372..2397)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(2398..2454)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(2464..4113)
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     regulatory      4110..4119
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        4741..5923
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     primer_bind     5871..5891
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     complement(6830..6847)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6836..7060
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        7422..7725
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7726..7929
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7880..7900
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     7926..7950
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          8067..8163
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     primer_bind     8220..8239
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8220..8238
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             8249..9271
                     /codon_start=1
                     /product="hyperactive variant of the Sleeping Beauty 
                     transposase (Mates et al., 2009)"
                     /label=SB100X
                     /translation="MGKSKEISQDLRKRIVDLHKSGSSLGAISKRLAVPRSSVQTIVRK
                     YKHHGTTQPSYRSGRRRVLSPRDERTLVRKVQINPRTTAKDLVKMLEETGTKVSISTVK
                     RVLYRHNLKGHSARKKPLLQNRHKKARLRFATAHGDKDRTFWRNVLWSDETKIELFGHN
                     DHRYVWRKKGEACKPKNTIPTVKHGGGSIMLWGCFAAGGTGALHKIDGIMDAVQYVDIL
                     KQHLKTSVRKLKLGRKWVFQHDNDPKHTSKVVAKWLKDNKVKVLEWPSQSPDLNPIENL
                     WAELKKRVRARRPTNLTQLHQLCQEEWAKIHPNYCGKLVEGYPKRLTQVKQFKGNATKY
                     "
     polyA_signal    9288..9422
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9312..9331)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     9366..9385
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        9575..9679
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9680..9748,9749..10540)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9898..9917)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggatc cctatacagt
      781 tgaagtcgga agtttacata caccttagcc aaatacattt aaactcactt tttcacaatt
      841 cctgacattt aatcctagta aaaattccct gtcttaggtc agttaggatc accactttat
      901 tttaagaatg tgaaatatca gaataatagt agagagaatg attcatttca gcttttattt
      961 ctttcatcac attcccagtg ggtcagaagt ttacatacac tcgactcctc tgcagaatgc
     1021 ggcgatgttt cggtaagggg tcccgagtag ctagttcatg gcagccagca tatggcatat
     1081 gttgccaaac tctaaaccaa atactcattc tgatgtttta aatgatttgc cctcccatat
     1141 gtccttccga gtgagagaca caaaaaattc caacacacta ttgcaatgaa aataaatttc
     1201 ctttattagc cagaagtcag atgctcaagg ggcttcatga tgtccccata atttttggca
     1261 gagggaaaaa gatctcagtg gtatttgtga gccagggcat tggccacacc agccaccacc
     1321 ttctgatagg cagcctgcac ctgaggagtg aattcacgac aggccttcga actattcctt
     1381 tgccctcgga cgagtgctgg ggcgtcggtt tccactatcg gcgagtactt ctacacagcc
     1441 atcggtccag acggccgcgc ttctgcgggc gatttgtgta cgcccgacag tcccggctcc
     1501 ggatcggacg attgcgtcgc atcgaccctg cgcccaagct gcatcatcga aattgccgtc
     1561 aaccaagctc tgatagagtt ggtcaagacc aatgcggagc atatacgccc ggagccgcgg
     1621 cgatcctgca agctccggat gcctccgctc gaagtagcgc gtctgctgct ccatacaagc
     1681 caaccacggc ctccagaaga agatgttggc gacctcgtat tgggaatccc cgaacatcgc
     1741 ctcgctccag tcaatgaccg ctgttatgcg gccattgtcc gtcaggacat tgttggagcc
     1801 gaaatccgcg tgcacgaggt gccggacttc ggggcagtcc tcggcccaaa gcatcagctc
     1861 atcgagagcc tgcgcgacgg acgcactgac ggtgtcgtcc atcacagttt gccagtgata
     1921 cacatgggga tcagcaatcg cgcatatgaa atcacgccat gtagtgtatt gaccgattcc
     1981 ttgcggtccg aatgggccga acccgctcgt ctggctaaga tcggccgcag cgatcgcatc
     2041 catggcctcc gcgaccggct gcagaacagc gggcagttcg gtttcaggca ggtcttgcaa
     2101 cgtgacaccc tgtgcacggc gggagatgca ataggtcagg ctctcgctga attccccaat
     2161 gtcaagcact tccggaatcg ggagcgcggc cgatgcaaag tgccgataaa cataacgatc
     2221 tttgtagaaa ccatcggcgc agctatttac ccgcaggaca tatccacgcc ctcctacatc
     2281 gaagctgaaa gcacgagatt cttcgccctc cgagagctgc atcaggtcgg agacgctgtc
     2341 gaacttttcg atcagaaact tctcgacaga cgtcgcggtg agttcaggct ttttcatggg
     2401 gccaggattc tcctcgacgt caccagcctg cttcagcagg ctgaagttag tagctccact
     2461 gcccacggcg atcttgccgc ccttcttggc cttaatgaga atctcgcgga tcttgcgggc
     2521 gtccaacttg ccggtcagtc ctttaggcac ctcgtccacg aacacaacac caccgcgcag
     2581 cttcttggcg gttgtaacct ggctggccac atagtccacg atctccttct cggtcatggt
     2641 tttaccgtgt tccagcacga cgactgcggc gggcagctcg ccggcatcgt cgtcgggcag
     2701 gccggcgacc ccggcgtcga agatgttggg gtgttgcagc aggatgctct ccagttcggc
     2761 tggggctacc tggtagccct tgtatttgat caggctcttc agccggtcca cgatgaagaa
     2821 gtgctcgtcc tcgtcccagt aggcgatgtc gccgctgtgc agccagccgt ccttgtcgat
     2881 gagagcgttt gtagcctcgg ggttgttaac gtagccgctc atgatcatgg ggccacggac
     2941 gcacagctcg ccgcgctggt tcacacccag tgtcttaccg gtgtccaagt ccaccacctt
     3001 agcctcgaag aagggcacca ccttgcctac tgcgccaggc ttgtcgtccc cttcgggggt
     3061 gatcagaatg gcgctggttg tttctgtcag gccgtagccc tggcggatgc ctggtaggtg
     3121 gaagcgtttg gccacggcct cacctacctc cttgctgagc ggcgccccgc cgctggcgat
     3181 ctcgtgcaag ttgcttaggt cgtacttgtc gatgagagtg ctcttagcga agaagctaaa
     3241 tagtgtgggc accagcaggg cagattgaat cttatagtct tgcaagctgc gcaagaatag
     3301 ctcctcctcg aagcggtaca tgagcacgac ccgaaagccg cagatcaagt agcccagcgt
     3361 ggtgaacatg ccgaagccgt ggtgaaatgg caccacgctg aggatagcgg tgtcggggat
     3421 gatctggttg ccgaagatgg ggtcgcgggc atgactgaat cggacacaag cggtgcggtg
     3481 cggtagggct acgcccttgg gcaatccggt actgccacta ctgttcatga tcagggcgat
     3541 ggttttgtcc cggtcgaagc tctcgggcac gaagtcgtac tcgttgaagc cgggtggcaa
     3601 atgggaagtc acgaaggtgt acatgctttg gaagccctgg tagtcggtct tgctatccat
     3661 gatgatgatc ttttgtatga tcggtagctt cttttgcacg ttgaggatct tttgcagccc
     3721 tttcttgctc acgaatacga cggtgggctg gctgatgccc atgctgttca gcagctcgcg
     3781 ctcgttgtag atgtcgttag ctggggccac agccacaccg atgaacaggg cacccaacac
     3841 gggcatgaag aactgcaagc tattctcgct gcacaccacg atccgatggt ttgtattcag
     3901 cccatagcgc ttcatagctt ctgccagccg aacgctcatc tcgaagtact cggcgtaggt
     3961 aatgtccacc tcgatatgtg cgtcggtaaa ggcgatggtg ccgggcacca gggcgtagcg
     4021 cttcatggct ttgtgcagct gctcgccggc ggtcccgtct tcgagtgggt agaatggcgc
     4081 tgggcccttc ttaatgtttt tggcatcttc catggtggcg gtgcagaggt ttctacaggg
     4141 aaagagagaa aggctacagt tagtttgctg tgatgatgct aagtgccgag gtcagatgtc
     4201 tatggattgt acacggaatc ccaacgcagg aaccaaaaaa cactcaccca aaaaaatggc
     4261 gctaaggccg agagaaaggc gaagttccgt ctacggctat ataccttgaa acaggggcgg
     4321 cagaagtgac gcaacgcggt tgcgcggacg gcttcctgtg ttttcattgg tcgaagcgtt
     4381 ctgcgcgtgc gcagagattc cccagggaag gcgggccctc ttccgaatgt ccaatccgga
     4441 cattcttccg gttggacctc cgcggtcagc gctgtgcccg gcgagagatc acgtggggcg
     4501 cggaggcggt gctgctgggg cacggccgtc cagcctcggc ggccatattt ttgaggggct
     4561 gttcatctcg ttcacacgct ctgtccgcca tgtttgtgag tggaagcgcc attacccctt
     4621 caagcgactg aaggctgcag ggcctctggt ggcccgcatg gggagaccag acccgccagg
     4681 cccgcctttc cgcactcagt ccgggcttac tttattttgt gagacagggt ctcgcctaga
     4741 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
     4801 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
     4861 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
     4921 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
     4981 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
     5041 acttccacgc ccctggctgc agtacgtgat tcttgatccc gagcttcggg ttggaagtgg
     5101 gtgggagagt tcgaggcctt gcgcttaagg agccccttcg cctcgtgctt gagttgaggc
     5161 ctggcttggg cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc
     5221 tgctttcgat aagtctctag ccatttaaaa tttttgatga cctgctgcga cgcttttttt
     5281 ctggcaagat agtcttgtaa atgcgggcca agatctgcac actggtattt cggtttttgg
     5341 ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct
     5401 gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa gctggccggc ctgctctggt
     5461 gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc
     5521 accagttgcg tgagcggaaa gatggccgct tcccggccct gctgcaggga gctcaaaatg
     5581 gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc acacaaagga aaagggcctt
     5641 tccgtcctca gccgtcgctt catgtgactc cacggagtac cgggcgccgt ccaggcacct
     5701 cgattagttc tcgagctttt ggagtacgtc gtctttaggt tggggggagg ggttttatgc
     5761 gatggagttt ccccatactg agtgggtgga gactgaagtt aggccagctt ggcacttgat
     5821 gtaattctcc ttggaatttg ccctttttga gtttggatct tggttcattc tcaagcctca
     5881 gacagtggtt caaagttttt ttcttccatt taaggtgtcg tgaaaactac cccaagctgg
     5941 cctctgcggc cgccaccatg atttggattt tgcttgtgct tgcatttatg ttctcctggc
     6001 atcttctgaa tgccttttct gtatctgttc ccaaagagaa atataaaaca cattatggca
     6061 gcaatgtgac tatggaatgc acctttccag tgggagaaaa attagacctc aatgccctta
     6121 cagtttattg ggacaaggaa ggaaaatttc ttgtgaaatt tgttgatggt gaagaagatc
     6181 tcaaaattca aaaaagcaac tcaagattaa ggcatttgaa tgaccaacta tacaagggaa
     6241 aatctcttct tcatatcacc aacgtgacag taaaagatgc tggggcttac cgctgtatta
     6301 ttggttacgg gggagctgat tacaagtgga ttactttaac cgtcaatgcc ccatacaaca
     6361 aaataaacca gaggattaaa atggatccag tcacttctga atacgaaatc acatgtcagt
     6421 cagaaggtta tcctaaagca gaagtcatct ggaaaagtag agatcaggat ctaagtagca
     6481 aagctacagt gacatattca gttggaacgg aaaagctatt caacattaca agcactctca
     6541 gggtcaatgc cacagtgaat gaatctttcc aatgcttgtt tcaggaaagg gatactggaa
     6601 aaaatatttc agctcagatt gtcatcccag acaattatgc atcaactaag acagcctcaa
     6661 gaacttactt tgggacttta ggagctgtca ttttccttgg attgattctg atgcttcaga
     6721 gctttagaaa aaaacatgtg agaatgatag ccaaagagaa aaattacctt tccacataag
     6781 gcctgtcagg ccaagcttgg ggatcaattc tctagagctc gctgatcagc ctcgactgtg
     6841 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     6901 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     6961 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
     7021 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
     7081 agctgggcct actagatagc ggacccctta ccgaaacatc gccgcattct gcagaggagt
     7141 cgaacttagt gtatgtaaac ttctgaccca ctggaattgt gatacagtga attataagtg
     7201 aaataatctg tctgtaaaca attgttggaa aaatgacttg tgtcatgcac aaagtagatg
     7261 tcctaaccga cttgccaaaa ctattgtttg ttaacaagaa atttgtggag tagttgaaaa
     7321 acgagtttta atgactccaa cttaagtgta tgtaaacttc cgacttcaac tgtataggga
     7381 tccaatttta acaaaatatt cgagcttgca tgcctgcagg tcgttacata acttacggta
     7441 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     7501 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     7561 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
     7621 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
     7681 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
     7741 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
     7801 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
     7861 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
     7921 agcagagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac
     7981 ctccatagaa gacaccggga ccgatccagc ctccggactc tagaggatcc ggtactcgag
     8041 gaactgaaaa accagaaagt taactggtaa gtttagtctt tttgtctttt atttcaggtc
     8101 ccggatccgg tggtggtgca aatcaaagaa ctgctcctca gtggatgttg cctttacttc
     8161 taggcctgta cggaagtgtt acttctgctc taaaagctgc ggaattgtac ccgcggaatt
     8221 aatacgactc actataggga ctagtaccat gggaaaatca aaagaaatca gccaagacct
     8281 cagaaaaaga attgtagacc tccacaagtc tggttcatcc ttgggagcaa tttccaaacg
     8341 cctggcggta ccacgttcat ctgtacaaac aatagtacgc aagtataaac accatgggac
     8401 cacgcagccg tcataccgct caggaaggag acgcgttctg tctcctagag atgaacgtac
     8461 tttggtgcga aaagtgcaaa tcaatcccag aacaacagca aaggaccttg tgaagatgct
     8521 ggaggaaaca ggtacaaaag tatctatatc cacagtaaaa cgagtcctat atcgacataa
     8581 cctgaaaggc cactcagcaa ggaagaagcc actgctccaa aaccgacata agaaagccag
     8641 actacggttt gcaactgcac atggggacaa agatcgtact ttttggagaa atgtcctctg
     8701 gtctgatgaa acaaaaatag aactgtttgg ccataatgac catcgttatg tttggaggaa
     8761 gaagggggag gcttgcaagc cgaagaacac catcccaacc gtgaagcacg ggggtggcag
     8821 catcatgttg tgggggtgct ttgctgcagg agggactggt gcacttcaca aaatagatgg
     8881 catcatggac gcggtgcagt atgtggatat attgaagcaa catctcaaga catcagtcag
     8941 gaagttaaag cttggtcgca aatgggtctt ccaacacgac aatgacccca agcatacttc
     9001 caaagttgtg gcaaaatggc ttaaggacaa caaagtcaag gtattggagt ggccatcaca
     9061 aagccctgac ctcaatccta tagaaaattt gtgggcagaa ctgaaaaagc gtgtgcgagc
     9121 aaggaggcct acaaacctga ctcagttaca ccagctctgt caggaggaat gggccaaaat
     9181 tcacccaaat tattgtggga agcttgtgga aggctacccg aaacgtttga cccaagttaa
     9241 acaatttaaa ggcaatgcta ccaaatacta ggggccctaa ccgcggggat ccagacatga
     9301 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
     9361 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
     9421 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt
     9481 tttaacgctt acaatttcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     9541 caccgcatca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     9601 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     9661 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     9721 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     9781 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     9841 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     9901 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     9961 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
    10021 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
    10081 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
    10141 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
    10201 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
    10261 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
    10321 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
    10381 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
    10441 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
    10501 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
    10561 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
    10621 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
    10681 agaccccgta gaaaagatca aaggatcttc
//
