LOCUS       sequence-442072-        3978 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3978)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 3978)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC29A3_STOP
FEATURES             Location/Qualifiers
     source          1..3978
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     89..106
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(265..292)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(384..470)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     519..541
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     533..550
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     534..550
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    566..665
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2100..2199)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2216..2235)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2217..2235)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2240..2256)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2240..2256)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2358..2377)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2369..3178
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2436..2455)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3325..3913
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3814..3833
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
       61 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
      121 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
      181 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatacgc gtaccgctag
      241 ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt ctgcttagtt
      301 tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc ctccgggccg ttgcttcaca
      361 acgttcaaat ccgctcccgg cggatttgtc ctactcagga gagcgttcac cgacaaacaa
      421 cagataaaac gaaaggccca gtcttccgac tgagcctttc gttttatttg atgcctggca
      481 gttccctact ctcgcgttaa cgctagcatg gatgttttcc cagtcacgac gttgtaaaac
      541 gacggccagt cttaagctcg ggccccaaat aatgatttta ttttgactga tagtgacctg
      601 ttcgttgcaa cacattgatg agcaatgctt ttttataatg ccaactttgt acaaaaaagc
      661 aggctccacc atggccgtgg tgagcgagga cgatttccag cacagctcca actccaccta
      721 caggaccaca tctagctccc tgagggcaga ccaggaggcc ctgctggaga agctgctgga
      781 taggccacct ccaggactgc agaggccaga ggacagattt tgcggcacat acatcatctt
      841 cttttccctg ggcatcggct ctctgctgcc ttggaacttc tttatcaccg ccaaggagta
      901 ttggatgttc aagctgagaa attctagctc cccagcaaca ggagaggacc ctgagggctc
      961 cgatatcctg aattacttcg agtcttatct ggcagtggca agcaccgtgc catccatgct
     1021 gtgcctggtg gccaactttc tgctggtgaa tcgggtggcc gtgcacatca gagtgctggc
     1081 ctccctgacc gtgatcctgg ccatcttcat ggtcatcaca gccctggtga aggtggatac
     1141 ctctagctgg acaaggggct tctttgccgt gaccatcgtg tgcatggtca tcctgagcgg
     1201 cgcctccacc gtgttctcct ctagcatcta cggcatgaca ggctcctttc ctatgcgcaa
     1261 cagccaggcc ctgatctccg gaggagcaat gggaggcacc gtgtctgccg tggcaagcct
     1321 ggtggacctg gcagcatcct ctgatgtgcg gaatagcgcc ctggccttct ttctgaccgc
     1381 cacagtgttc ctggtgctgt gcatgggcct gtacctgctg ctgagcagac tggagtatgc
     1441 caggtactat atgaggccag tgctggcagc acacgtgttc tccggagagg aggagctgcc
     1501 acaggactct ctgagcgccc catctgtggc aagccggttt atcgattccc acaccccccc
     1561 tctgagacca atcctgaaga agacagcctc tctgggcttc tgcgtgacct acgtgttctt
     1621 tatcacatct ctgatctatc ccgccatctg taccaacatc gagagcctga ataagggctc
     1681 cggctctctg tggaccacaa agttctttat ccctctgacc acattcctgc tgtacaactt
     1741 tgcagacctg tgcggcaggc agctgacagc atggatccag gtgccaggcc ccaattctaa
     1801 ggccctgcca ggcttcgtgc tgctgaggac ctgcctgatc cctctgttcg tgctgtgcaa
     1861 ctaccagcca cgcgtgcacc tgaagacagt ggtgtttcag agcgacgtgt accccgccct
     1921 gctgagctcc ctgctgggac tgtctaatgg ctacctgagc accctggccc tgctgtatgg
     1981 ccctaagatc gtgccaagag agctggccga ggccacaggc gtggtcatga gcttctacgt
     2041 gtgcctggga ctgaccctgg gatccgcctg ttctacactg ctggtgcacc tgatctaaga
     2101 cccagctttc ttgtacaaag ttggcattat aagaaagcat tgcttatcaa tttgttgcaa
     2161 cgaacaggtc actatcagtc aaaataaaat cattatttgc catccagctg atatccccta
     2221 tagtgagtcg tattacatgg tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa
     2281 tctctgatgt tacattgcac aagataaaaa tatatcatca tgaacaataa aactgtctgc
     2341 ttacataaac agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcgaggcc
     2401 gcgattaaat tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt
     2461 cgggcaatca ggtgcgacaa tctatcgctt gtatgggaag cccgatgcgc cagagttgtt
     2521 tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa
     2581 ctggctgacg gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga
     2641 tgcatggtta ctcaccactg cgatccccgg aaaaacagca ttccaggtat tagaagaata
     2701 tcctgattca ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc
     2761 gattcctgtt tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca
     2821 atcacgaatg aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg
     2881 gcctgttgaa caagtctgga aagaaatgca taaacttttg ccattctcac cggattcagt
     2941 cgtcactcat ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg
     3001 ttgtattgat gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg
     3061 gaactgcctc ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat
     3121 tgataatcct gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc
     3181 agaattggtt aattggttgt aacactggca gagcattacg ctgacttgac gggacggcgc
     3241 aagctcatga ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc
     3301 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     3361 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     3421 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     3481 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     3541 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     3601 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     3661 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     3721 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     3781 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     3841 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     3901 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     3961 ttttgctcac atgttctt
//
