LOCUS       sequence-442073-        3840 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3840)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 3840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC37A4_STOP
FEATURES             Location/Qualifiers
     source          1..3840
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     85..102
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(261..288)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(380..466)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     515..537
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     529..546
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     530..546
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    562..661
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1958..2057)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2074..2093)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2075..2093)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2098..2114)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2098..2114)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2216..2235)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2227..3036
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2294..2313)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3183..3771
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3672..3691
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
       61 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
      121 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
      181 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atacgcgtac cgctagccag
      241 gaagagtttg tagaaacgca aaaaggccat ccgtcaggat ggccttctgc ttagtttgat
      301 gcctggcagt ttatggcggg cgtcctgccc gccaccctcc gggccgttgc ttcacaacgt
      361 tcaaatccgc tcccggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga
      421 taaaacgaaa ggcccagtct tccgactgag cctttcgttt tatttgatgc ctggcagttc
      481 cctactctcg cgttaacgct agcatggatg ttttcccagt cacgacgttg taaaacgacg
      541 gccagtctta agctcgggcc ccaaataatg attttatttt gactgatagt gacctgttcg
      601 ttgcaacaca ttgatgagca atgctttttt ataatgccaa ctttgtacaa aaaagcaggc
      661 tccaccatgg ccgcccaggg ctacggctac tatcggaccg tgatcttctc cgccatgttt
      721 ggcggctatt ctctgtacta tttcaacaga aagacattct cctttgtgat gccctctctg
      781 gtggaggaga tccctctgga caaggacgat ctgggcttca tcaccagctc ccagagcgcc
      841 gcctacgcca tcagcaagtt cgtgagcggc gtgctgtctg atcagatgag cgcccggtgg
      901 ctgttttcta gcggcctgct gctggtgggc ctggtgaaca tcttctttgc ctggtcctct
      961 acagtgcccg tgttcgccgc cctgtggttt ctgaatggac tggcacaggg actgggatgg
     1021 ccaccttgcg gcaaggtgct gagaaagtgg ttcgagcctt cccagtttgg cacctggtgg
     1081 gccatcctgt ccacatctat gaatctggca ggaggactgg gaccaatcct ggcaaccatc
     1141 ctggcccaga gctactcctg gaggagcaca ctggccctgt ccggcgccct gtgcgtggtg
     1201 gtgagcttcc tgtgcctgct gctgatccac aacgagccag cagacgtggg actgaggaat
     1261 ctggatccta tgccatccga gggcaagaag ggctctctga aggaggagag caccctgcag
     1321 gagctgctgc tgagccctta cctgtgggtg ctgtccacag gctatctggt ggtgttcggc
     1381 gtgaagacct gctgtacaga ctggggccag ttctttctga tccaggagaa gggacagtcc
     1441 gccctggtgg gaagctccta tatgtctgcc ctggaagtgg gaggactggt gggatctatc
     1501 gcagcaggct acctgagcga tagggccatg gccaaggccg gcctgtccaa ctatggcaat
     1561 ccacgccacg gcctgctgct gttcatgatg gccggcatga ccgtgtctat gtacctgttt
     1621 agggtgaccg tgacatctga cagcccaaag ctgtggatcc tggtgctggg cgccgtgttc
     1681 ggcttttcta gctatggacc aatcgccctg ttcggcgtga tcgcaaacga gagcgcccca
     1741 ccaaatctgt gcggcacctc ccacgcaatc gtgggactga tggcaaacgt gggaggcttc
     1801 ctggcaggac tgccctttag caccatcgcc aagcactact cctggtctac agccttttgg
     1861 gtggccgaag tgatctgtgc cgcctctacc gccgccttct ttctgctgcg gaatatcaga
     1921 acaaagatgg gccgggtgag caagaaggcc gagtaagacc cagctttctt gtacaaagtt
     1981 ggcattataa gaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa
     2041 aataaaatca ttatttgcca tccagctgat atcccctata gtgagtcgta ttacatggtc
     2101 atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa
     2161 gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg
     2221 ggtgttatga gccatattca acgggaaacg tcgaggccgc gattaaattc caacatggat
     2281 gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc
     2341 tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc
     2401 gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct
     2461 cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg
     2521 atccccggaa aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt
     2581 gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct
     2641 tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg
     2701 gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa
     2761 gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca
     2821 cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc
     2881 ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct
     2941 ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa
     3001 ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa ttggttgtaa
     3061 cactggcaga gcattacgct gacttgacgg gacggcgcaa gctcatgacc aaaatccctt
     3121 aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     3181 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     3241 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     3301 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     3361 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     3421 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     3481 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     3541 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     3601 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     3661 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     3721 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     3781 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
//
