LOCUS       sequence-442078-        8473 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of HsXRN1-del1174-1649.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8473)
  AUTHORS   Chang CT, Muthukumar S, Weber R, Levdansky Y, Chen Y, Bhandari D, 
            Igreja C, Wohlbold L, Valkov E, Izaurralde E
  TITLE     A low-complexity region in human XRN1 directly recruits 
            deadenylation and decapping factors in 5'-3' messenger RNA decay.
  JOURNAL   Nucleic Acids Res. 2019 Sep 26;47(17):9282-9295. doi: 
            10.1093/nar/gkz633.
  PUBMED    31340047
REFERENCE   2  (bases 1 to 8473)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2019-09-26- 26"; volume: "47"; issue: 
            "17"; pages: "9282-9295"
COMMENT     Sequence Label: pT7-EGFP-C1-HsXRN1-del1174-1649_AH
FEATURES             Location/Qualifiers
     source          1..8473
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     613..632
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        613..631
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      659..668
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(665..667,668..670,671..1381)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(665..667,668..670,671..1381)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(710..731)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(971..990)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1318..1339
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    5261..5382
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5298..5317)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5352..5371
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(5389..5844)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5526..5547)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5738..5757
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5871..5975
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5977..6334
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5977..6168
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6000..6020)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6185..6320
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6369..7163
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6369..7163
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6423..6442)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7033..7052
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(7351..7370)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    7395..7442
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7771..8359
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8260..8279
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca ctgcttactg
      601 gcttatcgaa attaatacga ctcactatag ggagacccaa gctctagcgc taccggtcgc
      661 caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
      721 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
      781 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
      841 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
      901 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
      961 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     1021 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     1081 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     1141 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     1201 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     1261 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     1321 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     1381 gtccggactc agatctcgag ctcaagcttc gaattctgca gtcgacggta ccgcgggcat
     1441 gggagtcccc aagttttaca gatggatctc agagcggtat ccctgtctca gcgaagtggt
     1501 gaaagagcat cagattcctg aatttgacaa cttgtacctg gatatgaatg gaattataca
     1561 tcagtgctcc catcctaatg atgatgatgt tcactttaga atttcagatg ataaaatctt
     1621 tactgatatt tttcactacc tggaggtgtt gtttcgcatt attaaaccca ggaaagtgtt
     1681 ctttatggct gtagatggtg tggctcctcg agcaaaaatg aaccagcagc gtgggaggcg
     1741 ttttaggtca gcaaaggagg cagaagacaa aattaaaaag gcaatagaga agggagaaac
     1801 tcttcctaca gaggccagat ttgattccaa ctgtatcaca ccaggaactg aatttatggc
     1861 caggttacat gaacatctga agtattttgt aaatatgaaa atttccacag acaagtcatg
     1921 gcaaggagtt accatctact tctcaggcca tgagactcct ggagaaggag agcataaaat
     1981 catggaattt atcagatccg agaaagcaaa gccagatcat gatccaaaca ccagacactg
     2041 tctttatggt ttagatgctg acttgattat gcttggatta acaagtcatg aggcacattt
     2101 ttctctctta agagaagaag ttcgatttgg tggcaaaaaa acacaacggg tatgtgctcc
     2161 agaagaaact acatttcacc ttctacactt gtctttaatg agagagtata ttgactatga
     2221 gttttcagta ttaaaagaaa agatcacatt taaatatgat attgaaagga taatagatga
     2281 ttggattttg atggggtttc ttgttggtaa tgattttatc cctcatctac ctcatttaca
     2341 tattaatcat gatgcactgc ctcttcttta tggaacatat gttaccatcc tgccagaact
     2401 tgggggttat attaatgaaa gtgggcacct caacttacct cgatttgaga aataccttgt
     2461 gaaactatca gattttgatc gggagcactt cagtgaagtt tttgtggacc taaaatggtt
     2521 tgaaagcaaa gttggtaaca agtacctcaa tgaagcagca ggtgtcgcag cagaagaagc
     2581 caggaactac aaggaaaaga aaaagttaaa gggccaggaa aattctctgt gttggactgc
     2641 tttagacaaa aatgaaggcg aaatgataac ttctaaggat aatttagaag atgagactga
     2701 agatgatgac ctatttgaaa ctgagtttag acaatataaa agaacatatt acatgacgaa
     2761 gatgggggtt gacgtagtat ctgatgactt tctggctgat caagctgcat gttatgttca
     2821 ggcaatacag tggattttgc actattacta tcatggagtt cagtcctgga gctggtatta
     2881 tccttatcat tatgcacctt tcctgtctga tatacacaac atcagtacac tcaaaatcca
     2941 ttttgaacta ggaaaacctt ttaagccatt tgaacagctt cttgctgtac ttccagcagc
     3001 cagcaaaaat ttacttcctg catgctacca gcatttgatg accaatgaag actcaccaat
     3061 tatagaatat tacccacctg attttaaaac tgacctaaat gggaaacaac aggaatggga
     3121 agctgtggtg ttaatccctt ttattgatga gaagcgatta ttggaagcca tggagacatg
     3181 taaccactcc ctcaaaaagg aagagaggaa aagaaaccaa catagtgagt gcctaatgtg
     3241 ctggtatgat agagacacag agtttatcta tccttctcca tggccagaaa agttccctgc
     3301 catagaacga tgttgtacaa ggtataaaat aatatcctta gatgcttggc gtgtagacat
     3361 aaacaaaaac aaaataacca gaattgacca gaaagcatta tatttctgtg gatttcctac
     3421 tctgaaacac atcagacaca aatttttttt gaagaaaagt ggtgttcaag tattccagca
     3481 aagcagtcgt ggagaaaaca tgatgttgga aatcttagtg gatgcagaat cagatgaact
     3541 taccgtagaa aatgtagctt catcagtgct tggaaaatct gtctttgtta attggcctca
     3601 ccttgaggaa gctagagtcg tggctgtatc agatggagaa actaagtttt acttggaaga
     3661 acctccagga acacagaagc tttattcagg aagaactgcc ccaccatcta aagtggttca
     3721 tcttggagat aaagaacaat ctaactgggc aaaagaagta caaggaattt cagaacacta
     3781 cctgagaaga aaaggaataa taataaatga aacatctgca gttgtgtatg ctcagttact
     3841 cacaggtcgt aaatatcaaa taaatcaaaa tggtgaagtt cgtctagaga aacagtggtc
     3901 aaaacaagtt gttccttttg tttatcaaac tattgtcaag gacatccgag ctttcgactc
     3961 ccgtttctcc aatatcaaaa cattggatga tttgtttcct ctgagaagta tggtctttat
     4021 gctgggaact ccctattatg gctgcactgg agaagttcag gattcaggtg atgtgattac
     4081 agaaggtagg attcgtgtga ttttcagcat tccatgtgaa cccaatcttg atgctttaat
     4141 acagaaccag cataaatatt ctataaagta caacccagga tatgtgttgg ccagtcgcct
     4201 tggagtgagt ggataccttg tttcaaggtt tacaggaagt atttttattg gaagaggatc
     4261 taggagaaac cctcatggag accataaagc aaatgtgggt ttaaatctca aattcaacaa
     4321 gaaaaatgag gaggtacctg gatatactaa gaaagttgga agtgaatgga tgtattcatc
     4381 tgcagcagaa caacttctgg cagagtactt agagagagct ccagaactat ttagttatat
     4441 agccaaaaat agccaagagg atgtgttcta tgaagatgac atttggcctg gagaaaatga
     4501 gaatggtgct gaaaaagttc aagaaattat tacttggcta aaaggacatc ctgtcagtac
     4561 tttatctcgt tcttcttgtg atttacaaat tctggatgca gctattgttg agaaaattga
     4621 ggaagaagtc gaaaagtgca agcaaagaaa gaataataag aaggtgcgag taacagtgaa
     4681 accccatttg ctatacagac ctttagaaca gcaacatgga gtcattcctg atcgggatgc
     4741 agaattttgt ctttttgacc gtgttgtaaa tgtgagagaa aacttctcag ttccagttgg
     4801 ccttcgaggc accatcatag gaataaaagg agctaataga gaagccgatg tactatttga
     4861 agtattattt gatgaagaat ttcctggagg gttaacaata agatgctcac ctggtagagg
     4921 ttatcgactg ccaacaagtg ccttggtgaa cctttcttca tcagcttctt tgaagtcctc
     4981 tccgattgct caacctgcat cttcttttca agttgaaact gcctctcaag gccatagtat
     5041 atctcaccat aagtcaacac caatctcttc ttcaagaaga aaatcaagaa aactggctgt
     5101 taattttggt gtttctaaac cttctgagta aggatccacc ggatctagat aactgatcat
     5161 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
     5221 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
     5281 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     5341 gcattctagt tgtggtttgt ccaaactcat caatgtatct taacgcgtaa attgtaagcg
     5401 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
     5461 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
     5521 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
     5581 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
     5641 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
     5701 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
     5761 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
     5821 ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     5881 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     5941 gataaatgct tcaataatat tgaaaaagga agagtcctga ggcggaaaga accagctgtg
     6001 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     6061 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     6121 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     6181 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     6241 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     6301 aggaggcttt tttggaggcc taggcttttg caaagatcga tcaagagaca ggatgaggat
     6361 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
     6421 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     6481 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     6541 atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     6601 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     6661 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     6721 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     6781 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     6841 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca
     6901 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
     6961 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     7021 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     7081 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     7141 gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac
     7201 gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
     7261 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
     7321 gttcttcgcc caccctaggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg
     7381 aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt
     7441 tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca
     7501 ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga
     7561 aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcct caggttactc
     7621 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     7681 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     7741 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     7801 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     7861 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     7921 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     7981 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     8041 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     8101 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     8161 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     8221 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     8281 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     8341 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     8401 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     8461 taccgccatg cat
//
