LOCUS       sequence-442088-        8851 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmGIGYF_641-1574.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8851)
  AUTHORS   Ruscica V, Bawankar P, Peter D, Helms S, Igreja C, Izaurralde E
  TITLE     Direct role for the Drosophila GIGYF protein in 4EHP-mediated mRNA 
            repression.
  JOURNAL   Nucleic Acids Res. 2019 Jul 26;47(13):7035-7048. doi: 
            10.1093/nar/gkz429.
  PUBMED    31114929
REFERENCE   2  (bases 1 to 8851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2019-07-26- 26"; volume: "47"; issue: 
            "13"; pages: "7035-7048"
COMMENT     Sequence Label: pAc5.1B-EGFP-DmGIGYF_641-1574_AH
FEATURES             Location/Qualifiers
     source          1..8851
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..2510
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             join(2530..2532,2533..2535,2536..3246)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2530..2532,2533..2535,2536..3246)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2575..2596)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2836..2855)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3183..3204
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             6102..6143
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6153..6170
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6193..6210)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6243..6377
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6267..6286)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6321..6340
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(6978..7566)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7058..7077)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7737..8528,8529..8597))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8360..8379
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8598..8702)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8770..8788
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 catgaatggc atcaactctg aatcaaatct ttgcagatgc acctacttct catttccact
       61 gtcacatcat ttttccagat ctcgctgcct gttatgtggc ccacaaacca agacacgttt
      121 tatggccatt aaagctggct gatcgtcgcc aaacaccaaa tacataatga atatgtacac
      181 attcgagaaa gaagcgatca aagaagcgtc ttcgggcgga gtaggagaat gcggaggaga
      241 aggagaacga gctgatctag tatctctcca caatccaatg ccaactgacc aactggccat
      301 attcggagca atttgaagcc aatttccatc gcctggcgat cgctccattc ttggctatat
      361 gtttttcacc gttacccggg gccattttca aagactcgtc ggcaagataa gattgtgtca
      421 ctcgctgtct ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag
      481 tattttgaag aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac
      541 aaaaacatgt tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt
      601 atgtatatac ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac
      661 tgatgatatt ttaagatgat gccagaccaa aaggcttgaa tttctgcgtc ttttgccgaa
      721 cgcagtgcat gtgcaattgt tgttttttgg aatattcaat tttcggactg tccgctttga
      781 tttcagtttc ttggcttatt caaaaagcaa agtaaagcca aaaaagcgag atggcaatac
      841 caaatgcggc aaaacggtag tggaaggaaa ggggtgcggg gcagcggaag gaagggtggg
      901 gcggggcgtg gcggggtctg tggctgggcg cgacgtcacc gacgttggag ccactccttt
      961 gaccatgtgt gcgtgtgtgt attattcgtg tctcgccact cgccggttgt ttttttcttt
     1021 ttatgctgcg ctctctctag cgccatctcg cttacgcatg ctcaacgcac cgcatgttgc
     1081 cgtttccttt tatgcgtcat tttggctcga aataggcaat tatttaaaca aagattagtc
     1141 aacgaaaacg ctaaaataaa taagtctaca atatggttac ttattgccat gtgtgtgcag
     1201 ccaacgatag caacaaaagc aacaacacag gtggctttcc ctctttcact ttttgtttgc
     1261 aagccgcgtg cgagcaagac ggcacgaccg gcaaacgcaa ttacgctgac aaagagcaga
     1321 cgaagttttg gcgaaaaaca tcaaggcgcc tgatacgaat gcatttgcaa taacaattgc
     1381 gatatttaat attgtttatg aagctgtttg acttcaaaac acacaaaaaa aaaaataaaa
     1441 caaattattt gaaagagaat taggaatcgg acgcttatcg ttagggtaac aacaagaaat
     1501 gcttactgag tcacagcctc tggaaaactg ccgcaagcca gagagagaga gaaaaagagg
     1561 gagagcagct tagaccgcat gtgcttgtgt gtgaggcgtc tctctcttcg tctctgttgc
     1621 gcaaacgcat agactgcact gaaaaaatcg attacctatt ttttatgaat gaatatttgc
     1681 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1741 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1801 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1861 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgaatgcc ctactagaag
     1921 atgtgtgttg cacaaaatgt ccctggaata accaatttga agtgcagata gcagtaaacg
     1981 taagctaata tgaatattat ttaactgtaa tgttttaata tcgctggaca ttactaataa
     2041 acccactata aacacatgta catatgtatg ttttggcata caatgagtag ttggggaaaa
     2101 aatgtgtaaa agcaccgtga ccatcacagc ataaagataa ccagctgaag tatcgaatat
     2161 gagtaacccc caaattgaat cacatgccgc aactgatagg acccatggaa gtacactctt
     2221 catggcgata tacaagacac acacaagcac gaacacccag ttgcggagga aattctccgt
     2281 aaatgaaaac ccaatcggcg aacaattcat acccatatat ggtaaaagtt ttgaacgcga
     2341 cttgagagcg gagagcattg cggctgataa ggttttagcg ctaagcgggc tttataaaac
     2401 gggctgcggg accagttttc atatcactac cgtttgagtt cttgtgctgt gtggatactc
     2461 ctcccgacac aaagccgctc catcagccag cagtcgtcta atccagagac cccggatcgg
     2521 ggtaccatca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     2581 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     2641 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     2701 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac
     2761 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     2821 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     2881 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     2941 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
     3001 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
     3061 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     3121 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
     3181 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     3241 tacaagccaa gcttgaattc tattcctgtt ggtcaaatac cagcccctct aacggcgtca
     3301 ctttcaatta caccccataa gccatcaccg attcctatcg cactttctgt tgttgaacag
     3361 cagttgcagc aacaaagaga tgagcaacta aaggcaaatg ttacagcaac agctgaagct
     3421 ctacgtgctg caataaaagg aagttttggc ggaaatagca ttggtaatac gtctcatttg
     3481 cttacaatgc ggtttcaaat gctacaggat cagtacatac agcaccagga ataccaaata
     3541 ctagctgagc tatccaaaaa tgaatgcttt caacgtcttt cggctgtcga gcaagaaaca
     3601 gttgttcgtc ggaaagttca attgctgggt cttcctgagt atttgatcag cttaaacgga
     3661 ttaagcaatt ctttgtccgt actgaacccc gttgccggta gacagttgta cagggcagtg
     3721 gttgagcacg ccaagaagga tcaacaacat atttttgcaa acaccgagca acaacgttca
     3781 gtgggaaatt tactggatgc taataatttc attctaaatg cccaaataat gattcagcaa
     3841 tcgcagcaag aggtaggtcc cttggtatca tccgtggatt gtattatgca aggtggaact
     3901 gcagctgacc taaataagcc gaatgaaata ccgaggaatg agttagactt aattaatgaa
     3961 tataacttac ggatgctgtt gcgtggccaa ccaacaagta ctcaacagca accacctgcg
     4021 ctgacgaact cagctaccga aaatctccct ggagttgatt ttttaactga aacacaattg
     4081 ttagagcggc aaaacttgat gattccaatc tggttacccc ctaataataa taagcaacaa
     4141 gaaaccgacc aacagtgggc tgaaatgtct aatgcggatg catcattatg ggaaacggcc
     4201 aacttaaatg aagagcgaaa tgaagatcag caactattaa tacaaaagtc ttctgaagcg
     4261 tgcttcgcag atacagaaaa agatgtcaaa attgcccaat tatttcaagt tcaatcagga
     4321 aatgttgtta accacaccgc attagaggaa ttagatcagt cccctcaaaa tttaaaaggt
     4381 tcacataatc aaaaaatagt caaatccctt gtttccgata ttcaacagaa tcataatgag
     4441 gaactaaatt cacatcaaca ccaggtaaag caggctaaca agcagaatct gaatacaaaa
     4501 cagaatgcgg cacagtcagc cctaaagcca attaataacg aaaatgatcg caaacgagaa
     4561 cagacagaag aaaaaaaaag gcaaagagag gaacgcaagc gccagcaatt ggaagatgat
     4621 aaacgtcggg cgttgaatga atctgaagaa cagacccgcc aaattcaaga ggaaaaggaa
     4681 aggcaacagc aaatacaagc ccaacgtcga aaggcattgt tgggcaatgt ccattctcta
     4741 tcagttcaaa atgggatgtc aggaacatta gcgtctgcac aaagcaagaa gaacgacgat
     4801 gccaaaacag cagaacccca agtatcctcg cgcttgccgt ctacatccgt agcgccttgg
     4861 tcttttcagt tgcaaaattc tatgagatcg gcgcctggtc tggcagaaat acaaaaggca
     4921 gaacgtcgag agcgtcgcgc agaccaacag cgacatcaag agctattaga caagcaattg
     4981 cgtgccaatg ccgcagctgc ggctgaagcc aacgatgctt tgcttaaatg gcagtcaaca
     5041 ccagcgtcgg cgcccgtaat gagtctcgcc gaaattcaag cagaagaggc aaggcggttg
     5101 gccaatgacc ttgtggatcg gcagcgtcga cgagaactgg aacatcacca acaagctcct
     5161 ttgtcatcag cggttttggt tacgagtgca acttccaaca tctggggtaa cgccaataaa
     5221 gcatggagtt cgtccgctgc tcaatcactt tcgttaaaaa caagttctgg aactggtctg
     5281 tgggacgaac cgaatccatt aggttctaat ggatctggaa caagcggtac ctcatccgta
     5341 actgcggcgg cagtcttggc aggaggatta aactcagcta ataaatccac tctgcaagct
     5401 caaaataagt cgtcggcttt atttgcatcg cctcgaaatt tgcgcaagag tcaaacattg
     5461 ccagccctta gtaacccaga aaagtcaaat aaaaatggac caggtcaacg gccagaaaaa
     5521 caaaagttgg ctcaaactcg ttcaaagggt gccgctgtct caattgaaga gaaggataga
     5581 gaaagaaaat tgaatgcaaa aagccagcaa agcagcaccg accaagccat tagcaaggtt
     5641 aatgagtatg aaaacgagtt cactagctgg tgcataaaga gcctagataa tatgtccgcc
     5701 aaagttgatg tacccacgtt cgttgcattc ttgcaggact tggaagcgcc atatgaggta
     5761 aaggactatg tccgaatata ccttggcgat ggaaaagatt ctttggattt cgcaaagcag
     5821 tttttggagc gacgtagcaa atacaaaagc ttgcaacgtg cccaaaaggc acacaatgac
     5881 gatatgtgca aaccagctcc tgccattacg ccatctgcga atgactatgc cgacagcaag
     5941 aacaaacaaa aaaagattaa aaagaataag atgactaaga tggacgcccg cattcttggg
     6001 ttttcagtaa ctgctgccga gggtcgcata aatgttggca ttcgagacta cgtcgaagga
     6061 ccataagcgg ccgctcgagt ctagagggcc cgcggttcga aggtaagcct atccctaacc
     6121 ctctcctcgg tctcgattct acgcgtaccg gtcatcatca ccatcaccat tgagtttaaa
     6181 cccgctgatc agcctcgact gtgccttcta aggcctgagc tcgctgatca gcctcgatcg
     6241 aggatccaga catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt
     6301 gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa
     6361 gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg
     6421 aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg
     6481 atcagtcgac cgatgccctt gagagccttc aacccagtca gctccttccg gtgggcgcgg
     6541 ggcatgacta tcgtcgccgc acttatgact gtcttcttta tcatgcaact cgtaggacag
     6601 gtgccggcag cgctctgggt cattttcggc gaggaccgct ttcgctggag cgcgacgatg
     6661 atcggcctgt cgcttgcggt attcggaatc ttgcacgccc tcgctcaagc cttcgtcact
     6721 ggtcccgcca ccaaacgttt cggcgagaag caggccatta tcgccggcat ggcggccgac
     6781 gcgctgggct acgtcttgct ggcgttcgcg acgcgaggct ggatggcctt ccccattatg
     6841 attcttctcg cttccggcgg catcgggatg cccgcgttgc aggccatgct gtccaggcag
     6901 gtagatgacg accatcaggg acagcttcaa ggccagcaaa aggccaggaa ccgtaaaaag
     6961 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     7021 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     7081 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     7141 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     7201 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     7261 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     7321 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     7381 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     7441 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     7501 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     7561 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     7621 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     7681 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     7741 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     7801 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     7861 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     7921 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     7981 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     8041 gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     8101 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     8161 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     8221 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     8281 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     8341 tgcccggcgt caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     8401 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     8461 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     8521 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     8581 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     8641 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     8701 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     8761 acctataaaa ataggcgtat cacgaggccc tttcgtcttc aagaattaat tctatattct
     8821 aaaaacacaa atgatacttc taaaaaaaaa t
//
