LOCUS       sequence-44209-e        6156 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6156)
  AUTHORS   Watts KT, Lee PC, Schmidt-Dannert C
  TITLE     Biosynthesis of plant-specific stilbene polyketides in metabolically
            engineered Escherichia coli.
  JOURNAL   BMC Biotechnol. 2006 Mar 21;6:22.
  PUBMED    16551366
REFERENCE   2  (bases 1 to 6156)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2006-03-21"; volume: "6"; pages: "22"
COMMENT     Sequence Label: pAC-4CL1
FEATURES             Location/Qualifiers
     source          1..6156
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     134..153
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(220..322)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(714..731)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(848..1392)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(928..947)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(1504..1509,1510..1526,1527..1532)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     protein_bind    1909..1930
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1945..1950,1951..1968,1969..1975)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     complement(3844..3863)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             complement(join(5716..6156,1..219))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt
       61 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt
      121 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga
      181 tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga
      241 aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt
      301 ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc
      361 ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat
      421 ttattcggcg caaagtgcgt cgggtgatgc tgccaactta ctgatttagt gtatgatggt
      481 gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg
      541 acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctagcgg agtgtatact
      601 ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa
      661 aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc
      721 actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc
      781 ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa
      841 agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc
      901 agtggtggcg aaacccgaca ggactataaa gataccaggc gtttcccctg gcggctccct
      961 cgtgcgctct cctgttcctg cctttcggtt taccggtgtc attccgctgt tatggccgcg
     1021 tttgtctcat tccacgcctg acactcagtt ccgggtaggc agttcgctcc aagctggact
     1081 gtatgcacga accccccgtt cagtccgacc gctgcgcctt atccggtaac tatcgtcttg
     1141 agtccaaccc ggaaagacat gcaaaagcac cactggcagc agccactggt aattgattta
     1201 gaggagttag tcttgaagtc atgcgccggt taaggctaaa ctgaaaggac aagttttggt
     1261 gactgcgctc ctccaagcca gttacctcgg ttcaaagagt tggtagctca gagaaccttc
     1321 gaaaaaccgc cctgcaaggc ggttttttcg ttttcagagc aagagattac gcgcagacca
     1381 aaacgatctc aagaagatca tcttattaat cagataaaat atttcaagat ttcagtgcaa
     1441 tttatctctt caaatgtagc acctgaagtc agccccatac gatataagtt gtaattctca
     1501 tgtttgacag cttatcatcg ataagcttta atgcggtagt ttatcacagt taaattgcta
     1561 acgcagtcag gcaccgtgta tgaaatctaa caatgcgctc atcgtcatcc tcggcaccgt
     1621 caccctggat gctgtaggca taggcttggt tatgccggta ctgccgggcc tcttgcggga
     1681 tatcgtccat tccgacagca tcgccagtca ctatggcgtg ctgctagcgc tatatgcgtt
     1741 gatgcaattt ctatgcgcac ccgttctcgg agcactgtcc gaccgctttg gccgccgccc
     1801 agtcctgctc gcttcgctac ttggagccac tatcgactac gcgatcatgg cgaccacacc
     1861 cgtcctgtgg atccccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     1921 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
     1981 aattgtgagc gtctagagcg cccggggaat tccccatgga ggaggattac aaaatggcgc
     2041 cacaagaaca agcagtttct caggtgatgg agaaacagag caacaacaac aacagtgacg
     2101 tcattttccg atcaaagtta ccggatattt acatcccgaa ccacctatct ctccacgact
     2161 acatcttcca aaacatctcc gaattcgcca ctaagccttg cctaatcaac ggaccaaccg
     2221 gccacgtgta cacttactcc gacgtccacg tcatctcccg ccaaatcgcc gccaattttc
     2281 acaaactcgg cgttaaccaa aacgacgtcg tcatgctcct cctcccaaac tgtcccgaat
     2341 tcgtcctctc tttcctcgcc gcctccttcc gcggcgcaac cgccaccgcc gcaaaccctt
     2401 tcttcactcc ggcggagata gctaaacaag ccaaagcctc caacaccaaa ctcataatca
     2461 ccgaagctcg ttacgtcgac aaaatcaaac cacttcaaaa cgacgacgga gtagtcatcg
     2521 tctgcatcga cgacaacgaa tccgtgccaa tccctgaagg ctgcctccgc ttcaccgagt
     2581 tgactcagtc gacaaccgag gcatcagaag tcatcgactc ggtggagatt tcaccggacg
     2641 acgtggtggc actaccttac tcctctggca cgacgggatt accaaaagga gtgatgctga
     2701 ctcacaaggg actagtcacg agcgttgctc agcaagtcga cggcgagaac ccgaatcttt
     2761 atttccacag cgatgacgtc atactctgtg ttttgcccat gtttcatatc tacgctttga
     2821 actcgatcat gttgtgtggt cttagagttg gtgcggcgat tctgataatg ccgaagtttg
     2881 agatcaatct gctattggag ctgatccaga ggtgtaaagt gacggtggct ccgatggttc
     2941 cgccgattgt gttggccatt gcgaagtctt cggagacgga gaagtatgat ttgagctcga
     3001 taagagtggt gaaatctggt gctgctcctc ttggtaaaga acttgaagat gccgttaatg
     3061 ccaagtttcc taatgccaaa ctcggtcagg gatacggaat gacggaagca ggtccagtgc
     3121 tagcaatgtc gttaggtttt gcaaaggaac cttttccggt taagtcagga gcttgtggta
     3181 ctgttgtaag aaatgctgag atgaaaatag ttgatccaga caccggagat tctctttcga
     3241 ggaatcaacc cggtgagatt tgtattcgtg gtcaccagat catgaaaggt tacctcaaca
     3301 atccggcagc tacagcagag accattgata aagacggttg gcttcatact ggagatattg
     3361 gattgatcga tgacgatgac gagcttttca tcgttgatcg attgaaagaa cttatcaagt
     3421 ataaaggttt tcaggtagct ccggctgagc tagaggcttt gctcatcggt catcctgaca
     3481 ttactgatgt tgctgttgtc gcaatgaaag aagaagcagc tggtgaagtt cctgttgcat
     3541 ttgtggtgaa atcgaaggat tcggagttat cagaagatga tgtgaagcaa ttcgtgtcga
     3601 aacaggttgt gttttacaag agaatcaaca aagtgttctt cactgaatcc attcctaaag
     3661 ctccatcagg gaagatattg aggaaagatc tgagggcaaa actagcaaat ggattgtgac
     3721 catgggcggc cgctgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
     3781 ggatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg
     3841 gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga
     3901 gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca
     3961 tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg
     4021 gctgcttcct aatgcaggag tcgcataagg gagagcgtcg accgatgccc ttgagagcct
     4081 tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga
     4141 ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg gtcattttcg
     4201 gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg gtattcggaa
     4261 tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt ttcggcgaga
     4321 agcaggccat tatcgccggc atggcggccg acgcgctggg ctacgtcttg ctggcgttcg
     4381 cgacgcgagg ctggatggcc ttccccatta tgattcttct cgcttccggc ggcatcggga
     4441 tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga cgaccatcag ggacagcttc
     4501 aaggatcgct cgcggctctt accagcctaa cttcgatcac tggaccgctg atcgtcacgg
     4561 cgatttatgc cgcctcggcg agcacatgga acgggttggc atggattgta ggcgccgccc
     4621 tataccttgt ctgcctcccc gcgttgcgtc gcggtgcatg gagccgggcc acctcgacct
     4681 gaatggaagc cggcggcacc tcgctaacgg attcaccact ccaagaattg gagccaatca
     4741 attcttgcgg agaactgtga atgcgcaaac caacccttgg cagaacatat ccatcgcgtc
     4801 cgccatctcc agcagccgca cgcggcgcat ctcgggcagc gttgggtcct ggccacgggt
     4861 gcgcatgatc gtgctcctgt cgttgaggac ccggctaggc tggcggggtt gccttactgg
     4921 ttagcagaat gaatcaccga tacgcgagcg aacgtgaagc gactgctgct gcaaaacgtc
     4981 tgcgacctga gcaacaacat gaatggtctt cggtttccgt gtttcgtaaa gtctggaaac
     5041 gcggaagtcc cctacgtgct gctgaagttg cccgcaacag agagtggaac caaccggtga
     5101 taccacgata ctatgactga gagtcaacgc catgagcggc ctcatttctt attctgagtt
     5161 acaacagtcc gcaccgctgt ccggtagctc cttccggtgg gcgcggggca tgactatcgt
     5221 cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgcc
     5281 caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgccctgcac
     5341 cattatgttc cggatctgca tcgcaggatg ctgctggcta ccctgtggaa cacctacatc
     5401 tgtattaacg aagcgctaac cgtttttatc aggctctggg aggcagaata aatgatcata
     5461 tcgtcaatta ttacctccac ggggagagcc tgagcaaact ggcctcaggc atttgagaag
     5521 cacacggtca cactgcttcc ggtagtcaat aaaccggtaa accagcaata gacataagcg
     5581 gctatttaac gaccctgccc tgaaccgacg accgggtcga atttgctttc gaatttctgc
     5641 cattcatccg cttattatca cttattcagg cgtagcacca ggcgtttaag ggcaccaata
     5701 actgccttaa aaaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt
     5761 aagcattctg ccgacatgga agccatcaca gacggcatga tgaacctgaa tcgccagcgg
     5821 catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa
     5881 gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga
     5941 gacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca
     6001 cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca
     6061 gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc
     6121 ccatatcacc agctcaccgt ctttcattgc catacg
//
