LOCUS       sequence-44211-e         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbB8c-GFP
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      30..101
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      117..144
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(211..873)
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVLAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
ORIGIN
        1 tccaaactcg agtaaggatc tccaggcatc aaataaaacg aaaggctcag tcgaaagact
       61 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct
      121 caccttcggg tgggcctttc tgcgtttata cctaggctac agccgatagt ctggaacagc
      181 gcacttacgg gttgctgcgc aacccaagtg ctaccggcgc ggcagcgtga cccgtgtcgg
      241 cggctccaac ggctcgccat cgtccagaaa acacggctca tcgggcatcg gcaggcgctg
      301 ctgcccgcgc cgttcccatt cctccgtttc ggtcaaggct ggcaggtctg gttccatgcc
      361 cggaatgccg ggctggctgg gcggctcctc gccggggccg gtcggtagtt gctgctcgcc
      421 cggatacagg gtcgggatgc ggcgcaggtc gccatgcccc aacagcgatt cgtcctggtc
      481 gtcgtgatca accaccacgg cggcactgaa caccgacagg cgcaactggt cgcggggctg
      541 gccccacgcc acgcggtcat tgaccacgta ggccaacacg gtgccggggc cgttgagctt
      601 cacgacggag atccagcgct cggccaccaa gtccttgact gcgtattgga ccgtccgcaa
      661 agaacgtccg atgagcttgg aaagtgtctt ctggctgacc accacggcgt tctggtggcc
      721 catctgcgcc acgaggtgat gcagcagcat tgccgccgtg ggtttcctcg caataagccc
      781 ggcccacgcc tcatgcgctt tgcgttccgt ttgcacccag tgaccgggct tgttcttggc
      841 ttgaatgccg atttctctgg actgcgtggc catgcttatc
//
