LOCUS       sequence-44212-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbB8k-GFP
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(54..338)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     complement(112..131)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
ORIGIN
        1 tcatatgtat atctccttct taaaagatct tttgaattcc caaaaaaacg ggtatggaga
       61 aacagtagag agttgcgata aaaagcgtca ggtagaatcc gctaatctta tggataaaaa
      121 tgctatggca tagcaaagtg tgacgccgtg caaataatca atgtggactt ttctgccgtg
      181 attatagaca cttttgttac gcgtttttgt catggctttg gtcccgcttt gttacagaat
      241 gcttttaata agcggggtta ccggtttggt tagcgagaag agccagtaaa agacgcagtg
      301 acggcaatgt ctgatgcaat atggacaatt ggtttcttct ctgaatggcg ggagtatgaa
      361 aagtatggct gaagcgcaaa atgatcccct gctgccggga tactcgttta atgcccatct
      421 ggtggcgggt ttaacgccga ttgaggccaa cggttatctc gattttttta tcgaccgacc
      481 gctgggaatg aaaggttata ttctcaatct caccattcgc ggtcaggggg tggtgaaaaa
      541 tcagggacga gaatttgttt gccgaccggg tgatattttg ctgttcccgc caggagagat
      601 tcatcactac ggtcgtcatc cggaggctcg cgaatggtat caccagtggg tttactttcg
      661 tccgcgcgcc tactggcatg aatggcttaa ctggccgtca atatttgcca atacggggtt
      721 ctttcgcccg gatgaagcgc accagccgca tttcagcgac ctgtttgggc aaatcattaa
      781 cgccgggcaa ggggaagggc gctattcgga gctgctggcg ataaatctgc ttgagcaatt
      841 gttactgcgg cgcatggaag
//
