LOCUS       sequence-442123-        3927 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  The backbone vector for rice genome editing is based on HkCas12a.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3927)
  AUTHORS   Liu S, He Y, Fan T, Zhu M, Qi C, Ma Y, Yang M, Yang L, Tang X, Zhou 
            J, Zhong Z, An X, Qi Y, Zhang Y
  TITLE     PAM-relaxed and temperature-tolerant CRISPR-Mb3Cas12a single 
            transcript unit systems for efficient singular and multiplexed 
            genome editing in rice, maize, and tomato.
  JOURNAL   Plant Biotechnol J. 2024 Oct 10. doi: 10.1111/pbi.14486.
  PUBMED    39387219
REFERENCE   2  (bases 1 to 3927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Biotechnol J. 2024 Oct 10. doi: 10.1111/pbi.14486."
COMMENT     Sequence Label: pGEL1022
FEATURES             Location/Qualifiers
     source          1..3927
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ttcgagaagc tcagcaacat cgtgtccatt agcaagacaa tccgcttcaa gctcattcca
       61 gtgggcaaga ccctggagaa tatcgagaag ctgggcaagc tggagaagga cttcgagcgc
      121 tctgatttct accctatcct caagaacatt tccgacgatt actaccggca gtacatcaag
      181 gagaagctct cagacctgaa tctcgattgg cagaagctgt acgatgccca cgagctgctc
      241 gactcctcca agaaggaaag ccagaagaac ctggagatga tccaggcgca gtacaggaag
      301 gtcctgttca acattctctc aggcgagctg gacaagagcg gcgagaagaa ttcaaaggat
      361 ctgatcaaga acaataaggc cctctacggc aagctgttca agaagcagtt cattctggag
      421 gtgctccctg atttcgtcaa caataacgac tcatactctg aggaggacct ggagggcctg
      481 aacctctact ctaagttcac cacacgcctg aagaacttct gggagacacg gaagaatgtg
      541 ttcacagaca aggatatcgt caccgccatt ccgttccgcg cggtgaatga gaacttcggc
      601 ttctactacg acaacatcaa gattttcaat aagaacatcg agtacctgga gaataagatt
      661 cctaacctgg agaatgagct gaaggaggcc gacatcctgg acgataaccg ctcagtcaag
      721 gattacttca caccgaacgg cttcaattac gtgatcaccc aggacggcat tgatgtctac
      781 caggcgatca ggggcggctt cacaaaggag aacggcgaga aggtgcaggg catcaacgag
      841 attctgaatc tcacccagca gcagctcagg cgcaagccag agacaaagaa tgtgaagctg
      901 ggcgtcctca ccaagctgcg caagcagatc ctggagtact ccgagagcac atcattcctg
      961 atcgatcaga ttgaggacga taacgacctc gtggatcgga tcaacaagtt caatgtcagc
     1021 ttcttcgagt caaccgaggt gtctccttcc ctgttcgagc agatcgagcg gctctacaac
     1081 gccctcaagt ccatcaagaa ggaggaggtc tacattgacg cgaggaatac acagaagttc
     1141 tcacagatgc tgttcggcca gtgggatgtg atccggaggg gctacaccgt caagattaca
     1201 gagggctcta aggaggagaa gaagaagtac aaggagtacc tggagctgga cgagacatcc
     1261 aaggccaagc gctacctcaa catccgggag attgaggagc tggtgaatct ggtcgagggc
     1321 ttcgaggagg tggatgtgtt ctccgtgctc ctggagaagt tcaagatgaa taacatcgag
     1381 cgcagcgagt tcgaggcccc tatctacggc tccccgatca agctggaggc gatcaaggag
     1441 tacctggaga agcacctgga ggagtaccat aagtggaagc tcctgctcat cggcaacgac
     1501 gatctggaca ccgatgagac attctacccg ctgctcaatg aggtcatttc tgactactac
     1561 atcattccac tctacaacct gaccaggaat tacctcacac gcaagcactc cgacaaggat
     1621 aagatcaagg tgaacttcga cttcccaacc ctggccgatg gatggtcaga gtcaaagatt
     1681 tctgacaatc ggtccatcat tctgaggaag ggcggctact actacctcgg catcctgatt
     1741 gataacaagc tgctcatcaa caagaagaat aagtccaaga agatctacga gatcctcatc
     1801 tacaaccaga tcccggagtt ctctaagtcc attccgaatt acccattcac aaagaaggtc
     1861 aaggagcatt tcaagaataa cgtgagcgac ttccagctca tcgatggcta cgtgtcacca
     1921 ctgatcatta ccaaggagat ctacgacatt aagaaggaga agaagtacaa gaaggacttc
     1981 tacaaggata ataacacaaa taagaactac ctctacacca tctacaagtg gattgagttc
     2041 tgcaagcagt tcctgtacaa gtacaagggc ccgaacaagg agagctacaa ggagatgtac
     2101 gacttctcta ccctcaagga tacatccctc tacgtcaatc tgaacgactt ctacgccgat
     2161 gtgaactcat gcgcgtacag ggtcctgttc aataagattg acgagaacac aatcgataat
     2221 gccgtggagg acggcaagct gctcctgttc cagatctaca acaaggactt ctctccagag
     2281 tccaagggca agaagaatct ccacacactg tactggctca gcatgttctc agaggagaac
     2341 ctcaggaccc gcaagctgaa gctcaatggc caggcggaga tcttctaccg caagaagctg
     2401 gagaagaagc ctatcattca caaggagggc tctattctcc tgaacaagat cgataaggag
     2461 ggcaacacca tcccggagaa tatctaccat gagtgctacc gctacctcaa caagaagatc
     2521 ggccgcgagg acctgtccga tgaggcgatc gccctcttca acaaggacgt cctgaagtac
     2581 aaggaggcca ggttcgacat cattaaggat cgccggtaca gcgagtcaca gttcttcttc
     2641 catgtgccga tcacattcaa ctgggatatt aagaccaata agaacgtgaa tcagattgtc
     2701 cagggcatga tcaaggacgg cgagattaag cacatcattg gcatcgatag gggcgagcgc
     2761 catctcctgt actacagcgt catcgacctg gagggcaaca ttgtggagca gggctctctg
     2821 aataccctgg agcagaaccg cttcgacaat tccaccgtga aggtcgatta ccagaacaag
     2881 ctgaggacaa gggaggagga ccgcgatcgg gccaggaaga attggaccaa catcaataag
     2941 attaaggagc tgaaggatgg ctacctgtct cacgtggtcc ataagctgtc ccggctcatc
     3001 attaagtacg aggcgatcgt gattatggag aacctcaatc agggcttcaa gcgcggccgg
     3061 ttcaaggtgg agaggcaggt ctaccagaag ttcgagctgg ccctcatgaa caagctgtct
     3121 gcgctctcct tcaaggagaa gtacgacgag aggaagaacc tggagccatc cggcatcctg
     3181 aatcctattc aggcctgcta cccggtcgat gcgtaccagg agctgcaggg ccagaatggc
     3241 atcgtgttct acctgccagc cgcgtacacc tctgtgattg accctgtcac cggcttcaca
     3301 aacctgttca ggctcaagtc catcaattca tctaagtacg aggagttcat caagaagttc
     3361 aagaacatct acttcgataa tgaggaggag gacttcaagt tcatcttcaa ctacaaggac
     3421 ttcgccaagg cgaatctggt catcctcaat aacattaaga gcaaggattg gaagatctca
     3481 acacggggcg agaggattag ctacaactcc aagaagaagg agtacttcta cgtgcagcca
     3541 accgagttcc tcatcaataa gctgaaggag ctgaacatcg attacgagaa tattgacatc
     3601 attcctctca tcgacaacct ggaggagaag gccaagagga agattctcaa ggccctcttc
     3661 gataccttca agtacagcgt gcagctccgc aactacgact tcgagaatga ttacatcatt
     3721 agcccgacag ccgacgataa cggcaattac tacaactcaa atgagatcga cattgataag
     3781 accaatctcc caaataacgg cgacgccaac ggcgcgttca atatcgcgcg gaagggcctc
     3841 ctgctcaagg ataggattgt gaactctaat gagtccaagg tcgacctcaa gatcaagaac
     3901 gaggattgga ttaatttcat catttcc
//
