LOCUS       sequence-442125-        3780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  The Dual Pol II backbone vector for rice genome editing is based on 
            Mb3Cas12a.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3780)
  AUTHORS   Liu S, He Y, Fan T, Zhu M, Qi C, Ma Y, Yang M, Yang L, Tang X, Zhou 
            J, Zhong Z, An X, Qi Y, Zhang Y
  TITLE     PAM-relaxed and temperature-tolerant CRISPR-Mb3Cas12a single 
            transcript unit systems for efficient singular and multiplexed 
            genome editing in rice, maize, and tomato.
  JOURNAL   Plant Biotechnol J. 2024 Oct 10. doi: 10.1111/pbi.14486.
  PUBMED    39387219
REFERENCE   2  (bases 1 to 3780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Biotechnol J. 2024 Oct 10. doi: 10.1111/pbi.14486."
COMMENT     Sequence Label: pGEL1024
FEATURES             Location/Qualifiers
     source          1..3780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ctcttccagg acttcaccca cctctaccca ctgtccaaga cagtgcggtt cgagctgaag
       61 cctatcggca agaccctcga gcacattcat gccaagaact tcctcaatca ggacgagaca
      121 atggccgata tgtaccagaa ggtcaaggcg atcctggacg attaccatag ggacttcatt
      181 gccgatatga tgggcgaggt gaagctcacc aagctggcgg agttctacga tgtctacctg
      241 aagttccgca agaacccaaa ggacgatggc ctccagaagc agctcaagga cctccaggcc
      301 gtgctgcgga aggagatcgt caagcctatt ggcaatggcg gcaagtacaa ggccggctac
      361 gataggctgt tcggcgcgaa gctcttcaag gacggcaagg agctcggcga tctggccaag
      421 ttcgtgattg cccaggaggg agagtccagc cctaagctcg cccacctggc gcatttcgag
      481 aagttctcaa cctacttcac aggcttccac gacaacagga agaatatgta ctctgacgag
      541 gataagcaca ccgccatcgc gtaccggctg attcatgaga acctcccgag gttcattgat
      601 aatctccaga tcctggccac cattaagcag aagcattccg cgctgtacga ccagatcatt
      661 aacgagctca cagccagcgg cctcgatgtc tcactggcgt ctcacctcga cggctaccat
      721 aagctcctga cacaggaggg catcaccgcc tacaatacac tcctgggcgg catttcagga
      781 gaggccggca gccgcaagat ccagggcatt aacgagctca tcaattcaca ccataaccag
      841 cactgccata agtctgagag gatcgccaag ctccgcccgc tgcacaagca gattctgtcc
      901 gatggcatgg gcgtgtcctt cctcccaagc aagttcgccg acgatagcga ggtgtgccag
      961 gcggtcaacg agttctaccg gcattacgcc gacgtgttcg cgaaggtcca gtccctcttc
     1021 gatggcttcg acgattacca gaaggacggc atctacgtcg agtacaagaa cctcaatgag
     1081 ctgagcaagc aggccttcgg cgatttcgcc ctcctgggca gggtgctgga tggatactac
     1141 gtcgatgtgg tcaatccgga gttcaacgag cgcttcgcca aggcgaagac cgataacgcc
     1201 aaggcgaagc tcacaaagga gaaggacaag ttcattaagg gagtgcactc actggccagc
     1261 ctggagcagg ccattgagca ctacaccgcc aggcatgacg atgagtcagt gcaggccggc
     1321 aagctgggac agtacttcaa gcatggcctc gccggcgtgg acaacccaat ccagaagatt
     1381 cacaacaatc atagcacaat caagggcttc ctcgagcggg agcgcccggc cggcgagagg
     1441 gccctgccaa agatcaagtc cgataagtct ccagagattc ggcagctgaa ggaactcctg
     1501 gacaacgccc tcaatgtcgc ccacttcgcg aagctcctga ccacaaagac cacactccat
     1561 aatcaggatg gcaacttcta cggcgagttc ggcgcgctgt acgacgagct cgccaagatc
     1621 gcgaccctgt acaacaaggt gcgcgattac ctctcacaga agcctttctc taccgagaag
     1681 tacaagctca acttcggcaa tccgacactc ctgaatggct gggacctgaa caaggagaag
     1741 gataatttcg gcgtcatcct gcagaaggac ggctgctact acctcgccct cctggataag
     1801 gcgcacaaga aggtgttcga caacgccccg aatacaggca agagcgtcta ccagaagatg
     1861 atctacaagc tcctgccggg cccaaacaag atgctgccaa aggtgttctt cgcgaagtcc
     1921 aacctcgatt actacaatcc tagcgccgag ctcctggaca agtacgcgca gggcacccac
     1981 aagaagggcg acaacttcaa tctgaaggat tgccatgccc tcatcgactt cttcaaggcg
     2041 ggcattaata agcacccgga gtggcagcat ttcggcttca agttctcccc aacatcatct
     2101 taccaggacc tcagcgattt ctaccgcgag gtggagccac agggctacca ggtgaagttc
     2161 gtggacatca acgccgatta cattaatgag ctcgtcgagc agggccagct ctacctgttc
     2221 cagatctaca acaaggattt ctccccaaag gcccacggca agcctaatct ccataccctg
     2281 tacttcaagg cgctgttcag cgaggacaac ctcgtgaatc ctatctacaa gctcaacggc
     2341 gaggccgaga ttttctaccg gaaggcgtcc ctggatatga acgagacaac aattcacagg
     2401 gccggcgagg tgctcgagaa caagaatcct gacaacccga agaagcgcca gttcgtctac
     2461 gacatcatta aggataagcg gtacacacag gataagttca tgctccatgt gccgatcacc
     2521 atgaatttcg gcgtccaggg catgacaatt aaggagttca ataagaaggt gaaccagtca
     2581 atccagcagt acgatgaggt gaacgtcatt ggaattgatc gcggcgagag gcacctcctg
     2641 tacctcaccg tcatcaattc taagggcgag attctggagc agaggtcact caacgatatc
     2701 accacagcct ctgcgaatgg cacacagatg accacaccat accataagat cctggacaag
     2761 agggagattg agaggctcaa tgcccgggtg ggatggggcg agatcgagac aatcaaggag
     2821 ctgaagtcag gctacctctc tcacgtggtc catcagatca gccagctcat gctgaagtac
     2881 aacgccattg tggtcctcga ggacctgaat ttcggcttca agcggggcag gttcaaggtg
     2941 gagaagcaga tctaccagaa cttcgagaat gcgctgatta agaagctcaa ccacctcgtc
     3001 ctgaaggata aggccgacga tgagatcggc tcatacaaga atgcgctcca gctgaccaac
     3061 aatttcacag acctgaagtc tatcggcaag cagaccggct tcctcttcta cgtgcctgcc
     3121 tggaacacct caaagattga tccggagaca ggcttcgtgg acctcctgaa gcctcgctac
     3181 gagaacattg cccagtctca ggcgttcttc ggcaagttcg ataagatctg ctacaatgcg
     3241 gaccggggct acttcgagtt ccacattgat tacgccaagt tcaacgacaa ggcgaagaat
     3301 tccaggcaga tctggaagat ttgcagccat ggcgataagc gctacgtgta cgacaagacc
     3361 gccaaccaga ataagggcgc gacaatcggc gtgaacgtca atgatgagct caagtcactg
     3421 ttcacccggt accacattaa cgacaagcag ccaaatctcg tcatggatat ctgccagaac
     3481 aatgacaagg agttccataa gtctctgatg tacctcctga agacactcct ggccctcagg
     3541 tactcaaacg cctccagcga cgaggatttc atcctctctc ctgtggccaa cgatgagggc
     3601 gtcttcttca attcagcact ggccgatgat acacagccac agaatgcgga tgccaacggc
     3661 gcataccaca ttgccctcaa gggcctgtgg ctcctgaatg agctcaagaa ctcagacgat
     3721 ctgaacaagg tgaagctcgc cattgacaat cagacatggc tgaatttcgc gcagaaccgc
//
