LOCUS       sequence-44214-e        3622 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3622)
  AUTHORS   Watts KT, Lee PC, Schmidt-Dannert C
  TITLE     Biosynthesis of plant-specific stilbene polyketides in metabolically
            engineered Escherichia coli.
  JOURNAL   BMC Biotechnol. 2006 Mar 21;6:22.
  PUBMED    16551366
REFERENCE   2  (bases 1 to 3622)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2006-03-21"; volume: "6"; pages: "22"
COMMENT     Sequence Label: pUC-STS
FEATURES             Location/Qualifiers
     source          1..3622
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1262..1281)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1381..1403
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1441..1459)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1527..1631
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1632..1700,1701..2492)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1850..1869)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2663..3251
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3152..3171
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3405..3422
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3539..3560
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3575..3580,3581..3598,3599..3605)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 ctagaagaag gaggattaca aaatggtgtc tgtgagtgag atccgcaaag ttcaaagggc
       61 agaaggccct gcaactgtat tggcgatagg cacagcaaat ccaccaaatt gtattgatca
      121 gagcacatat gctgattatt attttagagt aactaacagt gaacacatga ctgatctcaa
      181 gaagaagttt cagcgcattt gtgagagaac acaaatcaag aacagacata tgtacttaac
      241 agaagagata ctgaaagaga atcctaacat gtgcgcatac aaagcaccgt cgttggatgc
      301 aagggaagac atgatgatca gggaggtacc aagggttgga aaagaggctg caaccaaggc
      361 catcaaggaa tggggtcagc caatgtctaa gatcacacat ttgatcttct gcaccaccag
      421 cggtgttgca ttgcctggcg ttgattacga actcatcgta ctcttaggac tcgacccatc
      481 cgtcaagagg tacatgatgt accaccaagg ctgcttcgcc ggtggcactg tccttcgttt
      541 ggctaaggac ttggctgaaa acaacaagga tgctcgtgtg cttatcgttt gttctgagaa
      601 taccgcagtc actttccgtg gtcctagtga gacagacatg gatagtcttg tagggcaagc
      661 cttgtttgct gatggagctg ctgcgattat cattggttct gatcctgtgc cagaggttga
      721 aaagcctatc tttgaaattg tttcgactga tcaaaaactt gtccctaaca gccatggagc
      781 catcggtggt ctccttcgtg aagttgggct tacattctat cttaataaga gtgttcctga
      841 tattatttca caaaacatca atgatgcgct cagtaaagct tttgatccat tgggtatatc
      901 tgattataac tcaatatttt ggattgcaca ccctggtgga cgtgcaattt tggatcaggt
      961 tgaacagaaa gtgaacttga aaccagagaa gatgaatgcc actagagacg tgcttagcaa
     1021 ttacggtaac atgtcaagtg cgtgtgtgtt cttcatcatg gatttaatga ggaagaagtc
     1081 ccttgaagaa ggacttaaaa ctaccggtga aggacttgat tggggcgtac tttttggctt
     1141 tggtcctggt ctcactattg aaactgttgt tctccgcagc atggccatat aagcggccgc
     1201 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca
     1261 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
     1321 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     1381 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
     1441 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     1501 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     1561 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     1621 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     1681 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     1741 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     1801 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     1861 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     1921 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     1981 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     2041 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     2101 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     2161 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     2221 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     2281 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     2341 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     2401 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     2461 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     2521 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     2581 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     2641 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     2701 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     2761 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     2821 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     2881 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     2941 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     3001 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     3061 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     3121 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     3181 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     3241 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     3301 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     3361 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     3421 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     3481 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     3541 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     3601 tgttgtgtgg aattgtgagc gt
//
