LOCUS       sequence-442144-        9286 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Zebrafish -2.1prox1a enhancer reporter in the lymphatic valve from 3
            dpf. XCA:DsRed2 as a control in the skeletal and cardiac muscle. 
            Shows the high background typical of the ZED vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9286)
  AUTHORS   Panara V, Yu H, Peng D, Staxang K, Hodik M, Filipek-Gorniok B, 
            Kazenwadel J, Skoczylas R, Mason E, Allalou A, Harvey NL, Haitina T,
            Hogan BM, Koltowska K
  TITLE     Multiple cis-regulatory elements control prox1a expression in 
            distinct lymphatic vascular beds.
  JOURNAL   Development. 2024 May 1;151(9):dev202525. doi: 10.1242/dev.202525. 
            Epub 2024 May 9.
  PUBMED    38722096
REFERENCE   2  (bases 1 to 9286)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2024-05-1"; volume: "151(9):dev202525. doi"; 
            pages: " 10.1242/dev.202525. Epub 2024 May 9"
COMMENT     Sequence Label: Zed_vector_tol2_-2.1prox1a_1:EGFP_XCA:DsRed2
FEATURES             Location/Qualifiers
     source          1..9286
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    join(15..26,27..214)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     protein_bind    complement(301..334)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     regulatory      1012..1021
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1018..1692
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(1198..1218)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     1610..1633
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    1817..1938
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1999..2015)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2610..2630)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2610..2628)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    complement(2633..2666)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     polyA_signal    2687..2808
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(3018..3731,3732..3734,3735..3737))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(3021..3731,3732..3734,3735..3737))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3063..3084)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     3412..3431
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3671..3692
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      3734..3743
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(4801..4817)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4820..4853)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    4862..4886
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5170..5194)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5786..5819)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    join(6186..6348,6349..6360)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     promoter        complement(6411..6429)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6412..6429)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6447..6463)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6447..6463)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6460..6482)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6471..6487
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6495..6501,6502..6519,6520..6525))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6540..6561
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6678..6695)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6849..7437)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6929..6948)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7608..8399,8400..8468))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8231..8250
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8469..8573)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8651..9106)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8788..8809)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9000..9019
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9232..9254
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9246..9263
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9247..9263
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(9270..9286,1..3)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        join(9270..9286,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggcgaattg ggcccagagg tgtaaagtac ttgagtaatt ttacttgatt actgtactta
       61 agtattattt ttggggattt ttactttact tgagtacaat taaaaatcaa tacttttact
      121 tttacttaat tacatttttt tagaaaaaaa agtacttttt actccttaca attttattta
      181 cagtcaaaaa gtacttattt tttggagatc acttcattct attttccctt gctattacca
      241 aaccaattga attgcgctga tgcccagttt aatttaaata gatctgtcga cttaattaat
      301 gaagttccta tactttctag agaataggaa cttcctcact atagggcgaa ttggagctcc
      361 accgcggtgg cggccgctct agaattctgc acattggctg tttcccaaca gagtgcaagc
      421 tctgagagca aatcttgccc gtcattactg tttgatataa ggtattccca tgcctgacgc
      481 acataatatt tttgttacat tcgtgtttac agcactgaag gcaggcagct atttgtgcag
      541 tcagtacttt atgtatttct aaacccagtg ctggagaggc caataggcag gatgcccccc
      601 ccgcgttagt ccccctgcac aattgtgctg cacctgtcta ctccatttgc agacccctgt
      661 gtctgtgcaa actatttctt tcattgtgct gttttttttg tcacccagca ttacagacat
      721 gcttttttgg gaatccctat ttggccatcc ctagtagtgc tccccccacc tccatacttt
      781 ccatacatgg gctaaggggt ccaaagaccc tgccctcccc cctcacctac tccattaatg
      841 gcttctttgc ttttcaatgg cccagaagct accaaataag ggcaggctgc ctgcctttcc
      901 ctgcggagct cccactgact cctcaactcc aggcagcgta taaattgaca gctcagtccc
      961 caggcagcac ccagccaatt caatcacagc acggggatcg atccaccggt cgccaccatg
     1021 gcctcctccg agaacgtcat caccgagttc atgcgcttca aggtgcgcat ggagggcacc
     1081 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggccac
     1141 aacaccgtga agctgaaggt gaccaagggc ggccccctgc ccttcgcctg ggacatcctg
     1201 tccccccagt tccagtacgg ctccaaggtg tacgtgaagc accccgccga catccccgac
     1261 tacaagaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac
     1321 ggcggcgtgg cgaccgtgac ccaggactcc tccctgcagg acggctgctt catctacaag
     1381 gtgaagttca tcggcgtgaa cttcccctcc gacggccccg tgatgcagaa gaagaccatg
     1441 ggctgggagg cctccaccga gcgcctgtac ccccgcgacg gcgtgctgaa gggcgagacc
     1501 cacaaggccc tgaagctgaa ggacggcggc cactacctgg tggagttcaa gtccatctac
     1561 atggccaaga agcccgtgca gctgcccggc tactactacg tggacgccaa gctggacatc
     1621 acctcccaca acgaggacta caccatcgtg gagcagtacg agcgcaccga gggccgccac
     1681 cacctgttcc tgagatcggc cgcgactcta gatcataatc agccatacca catttgtaga
     1741 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
     1801 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     1861 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     1921 actcatcaat gtatcttaag atcctagaac tagaactagt ggatcccccg ggctgcagga
     1981 attcgatatc aagcttatcg ataccgtcga cctcgagcgg ccgccagtgt gatggatatc
     2041 tgcagaattc ggcttacaca ctcaagctgg ttctttgaac agagtttaag aaagaagcta
     2101 tttacaatgt tgggggtgga atttaagaag aaaggacagt ggataatgaa aaggatactc
     2161 agaacaactg ctggaacaca gacatcactg tcttgacaaa gtcagctggc aggaactatg
     2221 accttggaga aggatataag caactttctt gttatgtggc aaagaggaag ttgcagacct
     2281 ttgacttcac tctccttctg cctctaaaag ccgaattcca gcacactggc ggccgttact
     2341 agtggatccc ccgggctgca ggaattcgat tatgtcaaaa acctgtactt cataaaattg
     2401 gaaatgacaa ttttcttgat aggtatcact taccaaagtt aaatgaaaat catattaaca
     2461 atttaaatag acctgtaata tataagaaaa taaaagcaat cattaaaaga ctccaaatcc
     2521 cccccggccc cccaaagaaa gcccaaagac tgttttagga cagaattcct gcagcccggg
     2581 ggatccgagc tcggtaccag cttttgttcc ctttagtgag ggttaattgc gtgaagttcc
     2641 tatactttct agagaatagg aacttcttaa ttaaccatgg cgtacgtaag atacattgat
     2701 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
     2761 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
     2821 tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa
     2881 aacctctaca aatgtggtat ggctgattat gatcagttat ctagatccgg tactagtagg
     2941 cctattattt ttgacaccag acaagttggt aatggtagcg accggcgctc agttggaatt
     3001 ctagagtcgc ggccgcttta cttgtacagc tcgtccatgc cgagagtgat cccggcggcg
     3061 gtcacgaact ccagcaggac catgtgatcg cgcttctcgt tggggtcttt gctcagggcg
     3121 gactgggtgc tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg
     3181 ttctgctggt agtggtcggc gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg
     3241 aagttcacct tgatgccgtt cttctgcttg tcggccatga tatagacgtt gtggctgttg
     3301 tagttgtact ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc
     3361 agctcgatgc ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag
     3421 ttgccgtcgt ccttgaagaa gatggtgcgc tcctggacgt agccttcggg catggcggac
     3481 ttgaagaagt cgtgctgctt catgtggtcg gggtagcggc tgaagcactg cacgccgtag
     3541 gtcagggtgg tcacgagggt gggccagggc acgggcagct tgccggtggt gcagatgaac
     3601 ttcagggtca gcttgccgta ggtggcatcg ccctcgccct cgccggacac gctgaacttg
     3661 tggccgttta cgtcgccgtc cagctcgacc aggatgggca ccaccccggt gaacagctcc
     3721 tcgcccttgc tcaccatggt ggcgaccggt ggatcctcaa gtgtccgcgc ttagaaaatg
     3781 cttggaaaag tttacttgac actacagacc cgaaagcacc actatagtcc gttagattat
     3841 taaaatatct gtcgttagct taatgcttca ggtggcgatg ctctgtttac tttctgagca
     3901 tgcttttatt tttgaaggag tgtagaggtc ctgcttcttt gcattcaagg gtggccaggg
     3961 acacctgtaa gagaataaca gatagaagca tctcagtgtt gacgttaaac acagtgcaag
     4021 acagatctta attcacacga cgacgcctca gttaatataa gtaggctaac ttttgtagtc
     4081 aatcaattaa atcgctcaat taaatgctcg caatttcaaa taactatcgg gaagtactca
     4141 cggcgcaaag ttgcatttca gcactccaat cctgcgttta tttttcccgt gttagtcctg
     4201 cgcaaagaat gttaatcctc aagatgcctg ttgacgttcg taattcgcca ctggtttaaa
     4261 ttgtccacag cccgattgtt aaagtctccc taaaactctg cgtctgtcta aagcggagag
     4321 aagtctcatt aatgaattgt gcctgtgtac tgtcggaact ggcgccagta gattccatat
     4381 cttacgaaca tgggcgggga cacgagctgg cagtgagcgc cctgggaacc aatagcagag
     4441 cgcgctgact gccattggct cggacagcat gctttccgaa atgccacggc gactgtcggc
     4501 ctactgctcc acatccagag agctgagaga ctcatcattc agcgcagcaa tttattgaga
     4561 tctttttacc ctttttagag cttcacgttg atggtgtgta atgatgtctt tttactcaag
     4621 tgctaacaga aaagcctaaa atctaagttg cagcctaatg ctatttttta aatttatttt
     4681 taggacatta aatgatgtga ataatataat tatagacttg caggattata tttaattttt
     4741 aattgtgcag aaatgtatta aaatgcctct attctattaa tgaatatcgc gcaagcttat
     4801 cgataccgtc gacctcgaga taacttcgta tagcatacat tatacgaagt tatcataatc
     4861 aacaagtttg tacaaaaaag caggctgaca aaaggaaagg cgtgttgttc agtcagtttc
     4921 catgtcgcaa gcaaatgtta ttgtgagtga ataaactggg aaggcaggat ttttgcagca
     4981 gtgaattttt tttccaaaac aggtgttcca acttattttg ttagcatctt tccccaaagg
     5041 cacgctcgga gtgcgagctt agtggaaaat acatgacaac attaaagcag tggtgtttgg
     5101 agaaggaaaa cgcctcttag gtgtagagga aacctgttta ttaacaccgc cacctctgat
     5161 ctctgtcaca cccagctttc ttgtacaaag tggttgatta tggagttggc gcgcctgtca
     5221 ttctaaatct ctctttcagc ctaaagcttt ttccccgtat ccccccaggt gtctgcaggc
     5281 tcaaagagca gcgagaagcg ttcagaggaa agcgatcccg tgccaccttc cccgtgcccg
     5341 ggctgtcccc gcacgctgcc ggctcgggga tgcgggggga gcgccggacc ggagcggagc
     5401 cccgggcggc tcgctgctgc cccctagcgg gggagggacg taattacatc cctgggggct
     5461 ttgggagggg gctgtccccg tgagctccca ggcgcgcctg tcattctaaa tctctctttc
     5521 agcctaaagc tttttccccg tatcccccca ggtgtctgca ggctcaaaga gcagcgagaa
     5581 gcgttcagag gaaagcgatc ccgtgccacc ttccccgtgc ccgggctgtc cccgcacgct
     5641 gccggctcgg ggatgcgggg ggagcgccgg accggagcgg agccccgggc ggctcgctgc
     5701 tgccccctag cgggggaggg acgtaattac atccctgggg gctttggggg ggggctgtcc
     5761 ccgtgagctc ccaggcgcgc aactcataac ttcgtatagc atacattata cgaagttatg
     5821 gcccatctgg cctgtgtttc agacaccagg gagtctctgc tcacgtttcc tgctatttgc
     5881 agcctctcta tcaagactaa tacacctctt cccgcatcgg ctgcctgtga gaggcttttc
     5941 agcactgcag gattgctttt cagccccaaa agagctaggc ttgacactaa caattttgag
     6001 aatcagcttc tactgaagtt aaatctgagg ttttacaact ttgagtagcg tgtactggca
     6061 ttagattgtc tgtcttatag tttgataatt aaatacaaac agttctaaag caggataaaa
     6121 ccttgtatgc atttcattta atgttttttg agattaaaag cttaaacaag aatctctagt
     6181 tttctttctt gcttttactt ttacttcctt aatactcaag tacaatttta atggagtact
     6241 tttttacttt tactcaagta agattctagc cagatacttt tacttttaat tgagtaaaat
     6301 tttccctaag tacttgtact ttcacttgag taaaattttt gagtactttt tacacctctg
     6361 ctcgaccata tgggagagct cccaacgcgt tggatgcata gcttgagtat tctatagtgt
     6421 cacctaaata gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     6481 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     6541 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     6601 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     6661 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     6721 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     6781 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     6841 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     6901 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     6961 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     7021 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     7081 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     7141 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     7201 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     7261 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     7321 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     7381 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     7441 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     7501 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     7561 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     7621 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     7681 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     7741 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     7801 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     7861 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     7921 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     7981 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     8041 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     8101 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     8161 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     8221 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     8281 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     8341 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     8401 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     8461 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     8521 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     8581 ccccgaaaag tgccacctga tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
     8641 ccgcatcagg aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
     8701 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
     8761 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
     8821 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
     8881 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
     8941 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
     9001 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
     9061 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccat tcgccattca
     9121 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg
     9181 cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac
     9241 gacgttgtaa aacgacggcc agtgaattgt aatacgactc actata
//
