LOCUS       sequence-44215-e         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Shuttle vector for us in AdEasy System. For expression of transgenes
            under a CMV promoter when a GFP tracer is desired..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  AUTHORS   He TC, Zhou S, da Costa LT, Yu J, Kinzler KW, Vogelstein B
  TITLE     A simplified system for generating recombinant adenoviruses.
  JOURNAL   Proc Natl Acad Sci U S A. 1998 Mar 3. 95(5):2509-14.
  PUBMED    9482916
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 1998 Mar 3. 95(5):2509-14."
COMMENT     Sequence Label: pAdTrack-CMV
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      17..26
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(65..268)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(94..114)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        269..572
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     polyA_signal    658..779
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(669..688)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     723..742
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ctctcgccct tgctcaccat ggtggcgacc ggtagcgcta gcggatctga cggttcacta
       61 aaccagctct gcttatatag acctcccacc gtacacgcct accgcccatt tgcgtcaatg
      121 gggcggagtt gttacgacat tttggaaagt cccgttgatt ttggtgccaa aacaaactcc
      181 cattgacgtc aatggggtgg agacttggaa atccccgtga gtcaaaccgc tatccacgcc
      241 cattgatgta ctgccaaaac cgcatcacca tggtaatagc gatgactaat acgtagatgt
      301 actgccaagt aggaaagtcc cataaggtca tgtactgggc ataatgccag gcgggccatt
      361 taccgtcatt gacgtcaata gggggcgtac ttggcatatg atacacttga tgtactgcca
      421 agtgggcagt ttaccgtaaa tactccaccc attgacgtca atggaaagtc cctattggcg
      481 ttactatggg aacatacgtc attattgacg tcaatgggcg ggggtcgttg ggcggtcagc
      541 caggcgggcc atttaccgta agttatgtaa cgcggaactc catatatggg ctatgaacta
      601 atgaccccgt aattgattac tattaatcac tagcagatcg tcgataaacc tccgcgttaa
      661 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
      721 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta
      781 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt
      841 aaagcaagta aaacctctac aaatgtggta tggctgatta tgatcagtt
//
