LOCUS       sequence-442161-        9913 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PUFFFIN cassette with s36GFP-mNeonGreen and mCherry-3NLS, strong 
            constitutive promoter CAG, insulated by cHS4 for random integration,
            puromycin resistance for selection in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9913)
  AUTHORS   Lebek T, Malaguti M, Boezio GL, Zoupi L, Briscoe J, Elfick A, Lowell
            S
  TITLE     PUFFFIN: an ultra-bright, customisable, single-plasmid system for 
            labelling cell neighbourhoods.
  JOURNAL   EMBO J. 2024 Jul 12. doi: 10.1038/s44318-024-00154-w.
  PUBMED    38997504
REFERENCE   2  (bases 1 to 9913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J. 
            2024 Jul 12. doi: 10.1038/s44318-024-00154-w."
COMMENT     Sequence Label: 
            pTL5_cHS4-CAG-s36GFPmNG-IRES-PuroR-p2AmCherry3NLS-polyA-cHS4
FEATURES             Location/Qualifiers
     source          1..9913
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1332..1635
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1637..1913
                     /label=chicken beta-actin promoter
     intron          1914..2930
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2854..2876
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2938..2957
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             3846..4553
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="VSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYEE
                     LNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFEDG
                     ASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIST
                     FKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQK
                     AFTDVMGMDELYK"
     regulatory      4098..4107
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(4745..4762)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4972..4991
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5117..5713
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5117..5136)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5613..5633
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             5720..5776
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5783..6490
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5928..5946)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6196..6215
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             6512..6532
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6536..6556
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6560..6580
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     6690..6706
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6690..6706
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     polyA_signal    6715..6763
                     /note="synthetic polyadenylation signal"
     terminator      8189..8218
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     rep_origin      8240..8827
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8728..8747
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(8839..8866)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(join(8890..9681,9682..9750))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESLRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLATIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGSLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9513..9532
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9751..9853)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(9854..9885)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 aatggtcagt attgagcgat atctagagaa ttcgtatagg gcgttaatta actagagccc
       61 catcctcact gactccgtcc tggagttgga tgagagataa tggccttacg ttgtgccagg
      121 ggagggtcgg gctggattta gcaagattta ccttctccaa agagcggtgc tgcagtggca
      181 cagctgccca cggaggtggg ggggtcaccg tccctggagg tgatgaagaa ctgtggggat
      241 gtggcactga gggacatggc cagtgggcac ggtgggtggg ttggggttgg tcttggggat
      301 cttggagggc ttttccagcc ttcatgattt gacgattgta tgaacatcta catggcaatt
      361 ctccagctgc ctgtcccagt cctactgacc cagctgtatc tctccaggca agctcttcca
      421 ccccttctgc ttgcatccag acaccatcaa acatgcaggc tcagacacag ggaccagcag
      481 tgtctgtggc ctttttgtgc tcctctccat gctgggtttt aacttgctct ttgtccttct
      541 atcctatctt cttatcctta aggctgttct gaacgctgtg acttggagag tgtcccagag
      601 ccctcaacac ctgcatgtcc cacgtccatg ctgtcctgca cttccttatc cccaagatct
      661 gcctctccgt gatgcactga attggcaaac atgtgtcacc ccagaccaac aatgtcacag
      721 caaactcccc cttgatagga caagggggaa tggctttaca ctgagacagg ggaggtttgg
      781 gttggatatg aggaggcagt ttttccccca gagggtggtg acgcactgaa caggttgccc
      841 aaggaggctg tggatgcccc atccctgcag gcattcaagg ccaggctgga tgtggctctg
      901 ggcagcctgg gctgctggtt gatgaccctg cacatagcag ggggttggat ctggatgagc
      961 actgtgctcc tttgcaaccc aggccgttct atgattctgt cattctaaat ctctctttca
     1021 gcctaaagct ttttccccgt atccccccag gtgtctgcag gctcaaagag cagcgagaag
     1081 cgttcagagg aaagcgatcc cgtgccacct tccccgtgcc cgggctgtcc ccgcacgctg
     1141 ccggctcggg gatgcggggg gagcgccgga ccggagcgga gccccgggcg gctcgctgct
     1201 gccccctagc gggggaggga cgtaattaca tccctggggg ctttgggggg gggctgtccc
     1261 tctagggacg ctagttatta atagtaatca attacggggt cattagttca tagcccatat
     1321 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
     1381 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
     1441 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
     1501 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
     1561 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
     1621 atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc catctccccc
     1681 ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg
     1741 gcgggggggg gggggggcgc gcgccrggsg ggggggggsg gggsgrgggg sggggsgggg
     1801 sgaggcggag aggtgcggcg gcagccaatc agagcggcgc gctccgaaag tttcctttta
     1861 tggcgaggcg gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg
     1921 ctgcgcgctg ccttcgcccc gtgccccgct ccgccgccgc ctcgcgccgc ccgccccggc
     1981 tctgactgac cgcgttactc ccacaggtga gcgggcggga cggcccttct cctccgggct
     2041 gtaattagcg cttggtttaa tgacggcttg tttcttttct gtggctgcgt gaaagccttg
     2101 aggggctccg ggagggccct ttgtgcgggg ggagcggctc ggggggtgcg tgcgtgtgtg
     2161 tgtgcgtggg gagcgccgcg tgcggctccg cgctgcccgg cggctgtgag cgctgcgggc
     2221 gcggcgcggg gctttgtgcg ctccgcagtg tgcgcgaggg gagcgcggcc gggggcggtg
     2281 ccccgcggtg cggggggggc tgcgagggga acaaaggctg cgtgcggggt gtgtgcgtgg
     2341 gggggtgagc agggggtgtg ggcgcgtcgg tcgggctgca accccccctg cacccccctc
     2401 cccgagttgc tgagcacggc ccggcttcgg gtgcggggct ccgtacgggg cgtggcgcgg
     2461 ggctcgccgt gccgggcggg gggtggcggc aggkgggggt gccgggcggg gcggggccgc
     2521 cycgggccgg ggrgggctcg ggggrggggc gcggcggccc ccggagcgcc ggcggctgtc
     2581 gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg gcgcagggac
     2641 ttcctttgtc ccaaatctgt gcggagccga aatctgggag gcgccgccgc accccctcta
     2701 gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg gagggccttc
     2761 gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc tcggggctgt ccgcgggggg
     2821 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
     2881 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
     2941 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attcctcgac tccgcctagg
     3001 cgcgccacct cgaggccgcc accatgaagt gggtgacctt catcagcctg ctgtttctgt
     3061 tcagcagcgc ctactctaga gggggcggag caagcaaagg cgagaggctg tttagaggga
     3121 aagtgccaat cctggtggaa ctgaaaggag acgtgaacgg ccacaagttc tctgtccgag
     3181 gcaaggggaa aggagatgcc acacggggaa aactgactct gaagtttatc tgcaccacag
     3241 gcaagctgcc cgtgccttgg ccaaccctgg tcactaccct gacatacggc gtgcagtgtt
     3301 tcagccgata tccaaaacac atgaagcggc atgacttctt taaaagtgct atgcccaagg
     3361 gctacgtgca ggagcgcacc atttcattca agaaggatgg gaagtacaag acccgggccg
     3421 aggtgaagtt tgaagggaga accctggtca accgcatcaa gctgaaagga cgcgacttca
     3481 aggaaaaagg gaatattctg ggacacaaac tgagatacaa cttcaacagc cataaggtgt
     3541 acatcacagc agataagaga aaaaacggca tcaaggccaa attcaagatt aggcacaatg
     3601 tgaaggacgg ctccgtccag ctggctgatc attaccagca gaacacacct attggcagag
     3661 ggcccgtgct gctgcctagg aatcattatc tgagcactcg ctccaaactg tctaaggacc
     3721 ccaaagagaa gcgggatcac atggtgctgc tggaatttgt caccgccgct ggcatcaaac
     3781 atgggcggga cgagagatac aagggcggca gcggcggcac cagcggcggc ggcggcggca
     3841 gcggcgtgag caagggcgag gaggataaca tggcctctct cccagcgaca catgagttac
     3901 acatctttgg ctccatcaac ggtgtggact ttgacatggt gggtcagggc accggcaatc
     3961 caaatgatgg ttatgaggag ttaaacctga agtccaccaa gggtgacctc cagttctccc
     4021 cctggattct ggtccctcat atcgggtatg gcttccatca gtacctgccc taccctgacg
     4081 ggatgtcgcc tttccaggcc gccatggtag atggctccgg ataccaagtc catcgcacaa
     4141 tgcagtttga agatggtgcc tcccttactg ttaactaccg ctacacctac gagggaagcc
     4201 acatcaaagg agaggcccag gtgaagggga ctggtttccc tgctgacggt cctgtgatga
     4261 ccaactcgct gaccgctgcg gactggtgca ggtcgaagaa gacttacccc aacgacaaaa
     4321 ccatcatcag tacctttaag tggagttaca ccactggaaa tggcaagcgc taccggagca
     4381 ctgcgcggac cacctacacc tttgccaagc caatggcggc taactatctg aagaaccagc
     4441 cgatgtacgt gttccgtaag acggagctca agcactccaa gaccgagctc aacttcaagg
     4501 agtggcaaaa ggcctttacc gatgtgatgg gcatggacga gctgtacaag taagcggccg
     4561 cttgatatcg aattccgccc ctctccctcc ccccccccta acgttactgg ccgaagccgc
     4621 ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt
     4681 ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt
     4741 tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg
     4801 gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca
     4861 cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg
     4921 gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc
     4981 tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct
     5041 gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaacgtctag
     5101 gccccccgaa cccgtcatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga
     5161 cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca
     5221 caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac
     5281 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt
     5341 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat
     5401 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc
     5461 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggtgtgtcgc ccgaccacca
     5521 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg
     5581 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg
     5641 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg
     5701 caagcccggt gcctcacttg ccacaaactt ctctctgcta aagcaagcag gtgatgttga
     5761 agaaaacccc gggcctgcct acatggtgag caagggcgag gaggataaca tggccatcat
     5821 caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga
     5881 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt
     5941 gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg
     6001 ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc
     6061 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac
     6121 ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa
     6181 cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga
     6241 gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa
     6301 ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca
     6361 gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta
     6421 caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga
     6481 gctgtacaag acctcaggca ggacaggtag tccaaaaaag aagagaaagg tagatccaaa
     6541 aaagaagaga aaggtagatc caaaaaagaa gagaaaggta ttataagcgg ccgctcgata
     6601 gcaaatgacc cgcaagcccg gtgcctgacg cccgccccac gacccgcagc gcccgaccga
     6661 aaggagcgca cgaccccatg catcgatgat ctagaactag tggatccccg atccaataaa
     6721 agatctttat tttcattaga tctgtgtgtt ggttttttgt gtgggcttgg gggaggggga
     6781 ggccagaatg actccaagag ctacaggaag gcaggtcaga gaccccactg gacaaacagt
     6841 ggctggactc tgcaccataa cacacaatca acaggggagt gagctggatc cggccctcta
     6901 gagccccatc ctcactgact ccgtcctgga gttggatgag agataatggc cttacgttgt
     6961 gccaggggag ggtcgggctg gatttagcaa gatttacctt ctccaaagag cggtgctgca
     7021 gtggcacagc tgcccacgga ggtggggggg tcaccgtccc tggaggtgat gaagaactgt
     7081 ggggatgtgg cactgaggga catggccagt gggcacggtg ggtgggttgg ggttggtctt
     7141 ggggatcttg gagggctttt ccagccttca tgatttgacg attgtatgaa catctacatg
     7201 gcaattctcc agctgcctgt cccagtccta ctgacccagc tgtatctctc caggcaagct
     7261 cttccacccc ttctgcttgc atccagacac catcaaacat gcaggctcag acacagggac
     7321 cagcagtgtc tgtggccttt ttgtgctcct ctccatgctg ggttttaact tgctctttgt
     7381 ccttctatcc tatcttctta tccttaaggc tgttctgaac gctgtgactt ggagagtgtc
     7441 ccagagccct caacacctgc atgtcccacg tccatgctgt cctgcacttc cttatcccca
     7501 agatctgcct ctccgtgatg cactgaattg gcaaacatgt gtcaccccag accaacaatg
     7561 tcacagcaaa ctcccccttg ataggacaag ggggaatggc tttacactga gacaggggag
     7621 gtttgggttg gatatgagga ggcagttttt cccccagagg gtggtgacgc actgaacagg
     7681 ttgcccaagg aggctgtgga tgccccatcc ctgcaggcat tcaaggccag gctggatgtg
     7741 gctctgggca gcctgggctg ctggttgatg accctgcaca tagcaggggg ttggatctgg
     7801 atgagcactg tgctcctttg caacccaggc cgttctatga ttctgtcatt ctaaatctct
     7861 ctttcagcct aaagcttttt ccccgtatcc ccccaggtgt ctgcaggctc aaagagcagc
     7921 gagaagcgtt cagaggaaag cgatcccgtg ccaccttccc cgtgcccggg ctgtccccgc
     7981 acgctgccgg ctcggggatg cggggggagc gccggaccgg agcggagccc cgggcggctc
     8041 gctgctgccc cctagcgggg gagggacgta attacatccc tgggggcttt gggggggggc
     8101 tgtccctcta gacgataacg aattcaagct tgatatcatt caggacgagc ctcagactcc
     8161 agcgtaactg gactgcaatc aactcactgg ctcaccttca cgggtgggcc tttcttcggt
     8221 agaaaatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     8281 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagctg ccaactcttt
     8341 ttccgaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     8401 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     8461 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     8521 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     8581 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     8641 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     8701 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     8761 gtttcgccac ctctgacttg agcatcgatt tttgtgatgc tcgtcagggg ggcggagcct
     8821 atggaaaaac gccagcaacg cagaaaggcc cacccgaagg tgagccaggt gattacattt
     8881 gggccctcat taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     8941 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatgcggg agggcttacc
     9001 atctggcccc agtgctgcaa tgataccgcg agaaccacgc tcaccggctc cagatttatc
     9061 agcaataaac cagccagccg ggagggccga gcgcagaagt gatcctgcaa ctttatccgc
     9121 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     9181 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     9241 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     9301 caaaaaagcg gttagctcct tcggtcctcc gatcgttgcc agaagtaagt tggccgcagt
     9361 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtgag
     9421 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     9481 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     9541 aaaagtgctc atcattggaa aacgttcttc ggggcgtaaa ctctcaagga tcttaccgct
     9601 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     9661 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     9721 aagggcgaca cggaaatgtt gaatactcat tttagcttcc ttagctcctg aaaatctcga
     9781 taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt tggaacctct
     9841 tacgtgccga tcaagtcaaa agcctccggt cggaggcttt tgactttctg ctatggaggt
     9901 caggtatgat tta
//
