LOCUS       sequence-442162-        9298 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PUFFFIN cassette with s36GFP-mNeonGreen and tagBFP, strong 
            constitutive promoter CAG, insulated by cHS4 for random integration,
            puromycin resistance for selection in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9298)
  AUTHORS   Lebek T, Malaguti M, Boezio GL, Zoupi L, Briscoe J, Elfick A, Lowell
            S
  TITLE     PUFFFIN: an ultra-bright, customisable, single-plasmid system for 
            labelling cell neighbourhoods.
  JOURNAL   EMBO J. 2024 Jul 12. doi: 10.1038/s44318-024-00154-w.
  PUBMED    38997504
REFERENCE   2  (bases 1 to 9298)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J. 
            2024 Jul 12. doi: 10.1038/s44318-024-00154-w."
COMMENT     Sequence Label: pTL50_cHS4-CAG-s36GFPmNG-t2A-PuroR-p2A-BFP-pol...
FEATURES             Location/Qualifiers
     source          1..9298
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1332..1635
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1637..1914
                     /label=chicken beta-actin promoter
     intron          1915..2931
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2855..2877
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2939..2958
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             3847..4551
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="VSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYEE
                     LNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFEDG
                     ASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIST
                     FKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQK
                     AFTDVMGMDELYK"
     regulatory      4099..4108
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4555..4566
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             4558..4611
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4618..5214
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4618..4637)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5114..5134
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             5221..5277
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5284..5985
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     primer_bind     6075..6091
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6075..6091
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     polyA_signal    6100..6148
                     /note="synthetic polyadenylation signal"
     terminator      7574..7603
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     rep_origin      7625..8212
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8113..8132
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(8224..8251)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(join(8275..9066,9067..9135))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESLRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLATIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGSLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8898..8917
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9136..9238)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(9239..9270)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 aatggtcagt attgagcgat atctagagaa ttcgtatagg gcgttaatta actagagccc
       61 catcctcact gactccgtcc tggagttgga tgagagataa tggccttacg ttgtgccagg
      121 ggagggtcgg gctggattta gcaagattta ccttctccaa agagcggtgc tgcagtggca
      181 cagctgccca cggaggtggg ggggtcaccg tccctggagg tgatgaagaa ctgtggggat
      241 gtggcactga gggacatggc cagtgggcac ggtgggtggg ttggggttgg tcttggggat
      301 cttggagggc ttttccagcc ttcatgattt gacgattgta tgaacatcta catggcaatt
      361 ctccagctgc ctgtcccagt cctactgacc cagctgtatc tctccaggca agctcttcca
      421 ccccttctgc ttgcatccag acaccatcaa acatgcaggc tcagacacag ggaccagcag
      481 tgtctgtggc ctttttgtgc tcctctccat gctgggtttt aacttgctct ttgtccttct
      541 atcctatctt cttatcctta aggctgttct gaacgctgtg acttggagag tgtcccagag
      601 ccctcaacac ctgcatgtcc cacgtccatg ctgtcctgca cttccttatc cccaagatct
      661 gcctctccgt gatgcactga attggcaaac atgtgtcacc ccagaccaac aatgtcacag
      721 caaactcccc cttgatagga caagggggaa tggctttaca ctgagacagg ggaggtttgg
      781 gttggatatg aggaggcagt ttttccccca gagggtggtg acgcactgaa caggttgccc
      841 aaggaggctg tggatgcccc atccctgcag gcattcaagg ccaggctgga tgtggctctg
      901 ggcagcctgg gctgctggtt gatgaccctg cacatagcag ggggttggat ctggatgagc
      961 actgtgctcc tttgcaaccc aggccgttct atgattctgt cattctaaat ctctctttca
     1021 gcctaaagct ttttccccgt atccccccag gtgtctgcag gctcaaagag cagcgagaag
     1081 cgttcagagg aaagcgatcc cgtgccacct tccccgtgcc cgggctgtcc ccgcacgctg
     1141 ccggctcggg gatgcggggg gagcgccgga ccggagcgga gccccgggcg gctcgctgct
     1201 gccccctagc gggggaggga cgtaattaca tccctggggg ctttgggggg gggctgtccc
     1261 tctagggacg ctagttatta atagtaatca attacggggt cattagttca tagcccatat
     1321 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
     1381 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
     1441 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
     1501 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
     1561 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
     1621 atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc catctccccc
     1681 ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg
     1741 gcgggggggg ggggggggcg cgcgccrggg gggggggggg ggggsgrggg gsggggsggg
     1801 gsgrggcgga gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt
     1861 atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc
     1921 gctgcgcgct gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg cccgccccgg
     1981 ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc tcctccgggc
     2041 tgtaattagc gcttggttta atgacggctt gtttcttttc tgtggctgcg tgaaagcctt
     2101 gaggggctcc gggagggccc tttgtgcggg gggagcggct cggggggtgc gtgcgtgtgt
     2161 gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga gcgctgcggg
     2221 cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc cgggggcggt
     2281 gccccgcggt gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg tgtgtgcgtg
     2341 ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct gcacccccct
     2401 ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg gcgtggcgcg
     2461 gggctcgccg tgccgggcgg ggggtggcgg caggkggggg tgccgggcgg ggcggggccg
     2521 ccycgggccg gggrgggctc gggggrgggg cgcggcggcc cccggagcgc cggcggctgt
     2581 cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat cgtgcgagag ggcgcaggga
     2641 cttcctttgt cccaaatctg tgcggagccg aaatctggga ggcgccgccg caccccctct
     2701 agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt
     2761 cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc ctcggggctg tccgcggggg
     2821 gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg tgtgaccggc
     2881 ggctctagag cctctgctaa ccatgttcat gccttcttct ttttcctaca gctcctgggc
     2941 aacgtgctgg ttattgtgct gtctcatcat tttggcaaag aattcctcga ctccgcctag
     3001 gcgcgccacc tcgaggccgc caccatgaag tgggtgacct tcatcagcct gctgtttctg
     3061 ttcagcagcg cctactctag agggggcgga gcaagcaaag gcgagaggct gtttagaggg
     3121 aaagtgccaa tcctggtgga actgaaagga gacgtgaacg gccacaagtt ctctgtccga
     3181 ggcaagggga aaggagatgc cacacgggga aaactgactc tgaagtttat ctgcaccaca
     3241 ggcaagctgc ccgtgccttg gccaaccctg gtcactaccc tgacatacgg cgtgcagtgt
     3301 ttcagccgat atccaaaaca catgaagcgg catgacttct ttaaaagtgc tatgcccaag
     3361 ggctacgtgc aggagcgcac catttcattc aagaaggatg ggaagtacaa gacccgggcc
     3421 gaggtgaagt ttgaagggag aaccctggtc aaccgcatca agctgaaagg acgcgacttc
     3481 aaggaaaaag ggaatattct gggacacaaa ctgagataca acttcaacag ccataaggtg
     3541 tacatcacag cagataagag aaaaaacggc atcaaggcca aattcaagat taggcacaat
     3601 gtgaaggacg gctccgtcca gctggctgat cattaccagc agaacacacc tattggcaga
     3661 gggcccgtgc tgctgcctag gaatcattat ctgagcactc gctccaaact gtctaaggac
     3721 cccaaagaga agcgggatca catggtgctg ctggaatttg tcaccgccgc tggcatcaaa
     3781 catgggcggg acgagagata caagggcggc agcggcggca ccagcggcgg cggcggcggc
     3841 agcggcgtga gcaagggcga ggaggataac atggcctctc tcccagcgac acatgagtta
     3901 cacatctttg gctccatcaa cggtgtggac tttgacatgg tgggtcaggg caccggcaat
     3961 ccaaatgatg gttatgagga gttaaacctg aagtccacca agggtgacct ccagttctcc
     4021 ccctggattc tggtccctca tatcgggtat ggcttccatc agtacctgcc ctaccctgac
     4081 gggatgtcgc ctttccaggc cgccatggta gatggctccg gataccaagt ccatcgcaca
     4141 atgcagtttg aagatggtgc ctcccttact gttaactacc gctacaccta cgagggaagc
     4201 cacatcaaag gagaggccca ggtgaagggg actggtttcc ctgctgacgg tcctgtgatg
     4261 accaactcgc tgaccgctgc ggactggtgc aggtcgaaga agacttaccc caacgacaaa
     4321 accatcatca gtacctttaa gtggagttac accactggaa atggcaagcg ctaccggagc
     4381 actgcgcgga ccacctacac ctttgccaag ccaatggcgg ctaactatct gaagaaccag
     4441 ccgatgtacg tgttccgtaa gacggagctc aagcactcca agaccgagct caacttcaag
     4501 gagtggcaaa aggcctttac cgatgtgatg ggcatggacg agctgtacaa gggtatagag
     4561 ggcagaggaa gtcttctaac atgcggtgac gtggaggaga atcccggccc tggcgtcatg
     4621 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc
     4681 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc
     4741 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc
     4801 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc
     4861 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc
     4921 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc
     4981 gcgtggttcc tggccaccgt cggtgtgtcg cccgaccacc agggcaaggg tctgggcagc
     5041 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag
     5101 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac
     5161 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctcactt
     5221 gccacaaact tctctctgct aaagcaagca ggtgatgttg aagaaaaccc cgggcctgcc
     5281 tacatgagcg agctgattaa ggagaacatg cacatgaagc tgtacatgga gggcaccgtg
     5341 gacaaccatc acttcaagtg cacatccgag ggcgaaggca agccctacga gggcacccag
     5401 accatgagaa tcaaggtggt cgagggcggc cctctcccct tcgccttcga catcctggct
     5461 actagcttcc tctacggcag caagaccttc atcaaccaca cccagggcat ccccgacttc
     5521 ttcaagcagt ccttccctga gggcttcaca tgggagagag tcaccacata cgaagacggg
     5581 ggcgtgctga ccgctaccca ggacaccagc ctccaggacg gctgcctcat ctacaacgtc
     5641 aagatcagag gggtgaactt cacatccaac ggccctgtga tgcagaagaa aacactcggc
     5701 tgggaggcct tcaccgaaac gctgtacccc gctgacggcg gcctggaagg cagaaacgac
     5761 atggccctga agctcgtggg cgggagccat ctgatcgcaa acatcaagac cacatataga
     5821 tccaagaaac ccgctaagaa cctcaagatg cctggcgtct actatgtgga ctacagactg
     5881 gaaagaatca aggaggccaa caacgagacc tacgtcgagc agcacgaggt ggcagtggcc
     5941 agatactgcg acctccctag caaactgggg cacaagctta attgagcaaa tgacccgcaa
     6001 gcccggtgcc tgacgcccgc cccacgaccc gcagcgcccg accgaaagga gcgcacgacc
     6061 ccatgcatcg atgatctaga actagtggat ccccgatcca ataaaagatc tttattttca
     6121 ttagatctgt gtgttggttt tttgtgtggg cttgggggag ggggaggcca gaatgactcc
     6181 aagagctaca ggaaggcagg tcagagaccc cactggacaa acagtggctg gactctgcac
     6241 cataacacac aatcaacagg ggagtgagct ggatccggcc ctctagagcc ccatcctcac
     6301 tgactccgtc ctggagttgg atgagagata atggccttac gttgtgccag gggagggtcg
     6361 ggctggattt agcaagattt accttctcca aagagcggtg ctgcagtggc acagctgccc
     6421 acggaggtgg gggggtcacc gtccctggag gtgatgaaga actgtgggga tgtggcactg
     6481 agggacatgg ccagtgggca cggtgggtgg gttggggttg gtcttgggga tcttggaggg
     6541 cttttccagc cttcatgatt tgacgattgt atgaacatct acatggcaat tctccagctg
     6601 cctgtcccag tcctactgac ccagctgtat ctctccaggc aagctcttcc accccttctg
     6661 cttgcatcca gacaccatca aacatgcagg ctcagacaca gggaccagca gtgtctgtgg
     6721 cctttttgtg ctcctctcca tgctgggttt taacttgctc tttgtccttc tatcctatct
     6781 tcttatcctt aaggctgttc tgaacgctgt gacttggaga gtgtcccaga gccctcaaca
     6841 cctgcatgtc ccacgtccat gctgtcctgc acttccttat ccccaagatc tgcctctccg
     6901 tgatgcactg aattggcaaa catgtgtcac cccagaccaa caatgtcaca gcaaactccc
     6961 ccttgatagg acaaggggga atggctttac actgagacag gggaggtttg ggttggatat
     7021 gaggaggcag tttttccccc agagggtggt gacgcactga acaggttgcc caaggaggct
     7081 gtggatgccc catccctgca ggcattcaag gccaggctgg atgtggctct gggcagcctg
     7141 ggctgctggt tgatgaccct gcacatagca gggggttgga tctggatgag cactgtgctc
     7201 ctttgcaacc caggccgttc tatgattctg tcattctaaa tctctctttc agcctaaagc
     7261 tttttccccg tatcccccca ggtgtctgca ggctcaaaga gcagcgagaa gcgttcagag
     7321 gaaagcgatc ccgtgccacc ttccccgtgc ccgggctgtc cccgcacgct gccggctcgg
     7381 ggatgcgggg ggagcgccgg accggagcgg agccccgggc ggctcgctgc tgccccctag
     7441 cgggggaggg acgtaattac atccctgggg gctttggggg ggggctgtcc ctctagacga
     7501 taacgaattc aagcttgata tcattcagga cgagcctcag actccagcgt aactggactg
     7561 caatcaactc actggctcac cttcacgggt gggcctttct tcggtagaaa atcaaaggat
     7621 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     7681 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aggtaactgg
     7741 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     7801 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     7861 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     7921 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     7981 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     8041 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     8101 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     8161 acttgagcat cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     8221 caacgcagaa aggcccaccc gaaggtgagc caggtgatta catttgggcc ctcattacca
     8281 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     8341 ctgactcccc gtcgtgtaga taactacgat gcgggagggc ttaccatctg gccccagtgc
     8401 tgcaatgata ccgcgagaac cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     8461 agccgggagg gccgagcgca gaagtgatcc tgcaacttta tccgcctcca tccagtctat
     8521 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     8581 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     8641 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     8701 ctccttcggt cctccgatcg ttgccagaag taagttggcc gcagtgttat cactcatggt
     8761 tatggcagca ctgcataatt ctcttactgt catgccatcc gtgagatgct tttctgtgac
     8821 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     8881 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     8941 tggaaaacgt tcttcggggc gtaaactctc aaggatctta ccgctgttga gatccagttc
     9001 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     9061 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     9121 atgttgaata ctcattttag cttccttagc tcctgaaaat ctcgataact caaaaaatac
     9181 gcccggtagt gatcttattt cattatggtg aaagttggaa cctcttacgt gccgatcaag
     9241 tcaaaagcct ccggtcggag gcttttgact ttctgctatg gaggtcaggt atgattta
//
