LOCUS       sequence-44217-e         912 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 912)
  TITLE     pLS/-11 combination of pLS1 encoding lactose repressor with deletion
            of 11 C-terminal amino acids
REFERENCE   2  (bases 1 to 912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLS1/-11
FEATURES             Location/Qualifiers
     source          1..912
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(124..140)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(124..140)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(137..159)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    148..164
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(172..178,179..196,197..202))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    217..238
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(262..279)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(513..532)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tccaccctga attgactctc ttccgggcgc tatcatgcca taccgcgaaa ggttttgcac
       61 cattcgatgg tgtcggaatt cccggggatc cgtcgacctg cagccaagct tggcgtaatc
      121 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
      181 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
      241 tgcgttgcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
      301 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
      361 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
      421 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
      481 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
      541 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
      601 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
      661 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
      721 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
      781 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
      841 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
      901 gaagccagtt ac
//
