LOCUS       sequence-442171-       10240 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Minimal PUFFFIN cassette with s36GFP-mNeonGreen and mCherry-3NLS, 
            separated by t2A self-cleaving peptide, strong constitutive promoter
            CMV, for lentivirus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10240)
  AUTHORS   Lebek T, Malaguti M, Boezio GL, Zoupi L, Briscoe J, Elfick A, Lowell
            S
  TITLE     PUFFFIN: an ultra-bright, customisable, single-plasmid system for 
            labelling cell neighbourhoods.
  JOURNAL   EMBO J. 2024 Jul 12. doi: 10.1038/s44318-024-00154-w.
  PUBMED    38997504
REFERENCE   2  (bases 1 to 10240)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J. 
            2024 Jul 12. doi: 10.1038/s44318-024-00154-w."
COMMENT     Sequence Label: pLenti6_cppt-CMV-Puffling-opre
FEATURES             Location/Qualifiers
     source          1..10240
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1058..1284
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1285..1465
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1512..1637
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2130..2363
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2548..2592
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2888..3005
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3084..3387
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3388..3591
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3542..3562
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3588..3612
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             4544..5248
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="VSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYEE
                     LNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFEDG
                     ASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIST
                     FKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQK
                     AFTDVMGMDELYK"
     regulatory      4796..4805
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5252..5263
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             5255..5308
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      5318..5327
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5324..6031
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5469..5487)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5737..5756
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             6053..6073
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6077..6097
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6101..6121
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        6752..7251
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6787..6810)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     7184..7203
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     promoter        7265..7312
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7331..7729
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             7816..8049
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8121..8242
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8158..8177)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8212..8231
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8282..8417
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8437..8456)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8438..8456)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8466..8483)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8466..8482)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8475..8497)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8624..9079
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8711..8730)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8921..8942
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9105..9209
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9210..9278,9279..10070)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9428..9447)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     1081 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     1141 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     1201 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     1261 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     1321 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     1381 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     1441 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     1501 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     1561 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     1621 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     1681 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     1741 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     1801 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     1861 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     1921 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     1981 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     2041 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     2101 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     2161 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     2221 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     2281 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     2341 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     2401 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     2461 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     2521 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     2581 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     2641 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     2701 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     2761 ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc
     2821 attcgattag tgaacggatc tcgacggtat cgatgtcgac agtacaaatg gcagtattca
     2881 tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag
     2941 acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa
     3001 attttcgggt ttattacagg gacagcagag atccagtttg gaaagggcta gcgactgggt
     3061 ctgcgataag cttgggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
     3121 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
     3181 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
     3241 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
     3301 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
     3361 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
     3421 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
     3481 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
     3541 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg
     3601 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccga
     3661 ctctagagga tctgttaaca tatgaattcg ctagcatgca tggatcctcg aggccgccac
     3721 catgaagtgg gtgaccttca tcagcctgct gtttctgttc agcagcgcct actctagagg
     3781 gggcggagca agcaaaggcg agaggctgtt tagagggaaa gtgccaatcc tggtggaact
     3841 gaaaggagac gtgaacggcc acaagttctc tgtccgaggc aaggggaaag gagatgccac
     3901 acggggaaaa ctgactctga agtttatctg caccacaggc aagctgcccg tgccttggcc
     3961 aaccctggtc actaccctga catacggcgt gcagtgtttc agccgatatc caaaacacat
     4021 gaagcggcat gacttcttta aaagtgctat gcccaagggc tacgtgcagg agcgcaccat
     4081 ttcattcaag aaggatggga agtacaagac ccgggccgag gtgaagtttg aagggagaac
     4141 cctggtcaac cgcatcaagc tgaaaggacg cgacttcaag gaaaaaggga atattctggg
     4201 acacaaactg agatacaact tcaacagcca taaggtgtac atcacagcag ataagagaaa
     4261 aaacggcatc aaggccaaat tcaagattag gcacaatgtg aaggacggct ccgtccagct
     4321 ggctgatcat taccagcaga acacacctat tggcagaggg cccgtgctgc tgcctaggaa
     4381 tcattatctg agcactcgct ccaaactgtc taaggacccc aaagagaagc gggatcacat
     4441 ggtgctgctg gaatttgtca ccgccgctgg catcaaacat gggcgggacg agagatacaa
     4501 gggcggcagc ggcggcacca gcggcggcgg cggcggcagc ggcgtgagca agggcgagga
     4561 ggataacatg gcctctctcc cagcgacaca tgagttacac atctttggct ccatcaacgg
     4621 tgtggacttt gacatggtgg gtcagggcac cggcaatcca aatgatggtt atgaggagtt
     4681 aaacctgaag tccaccaagg gtgacctcca gttctccccc tggattctgg tccctcatat
     4741 cgggtatggc ttccatcagt acctgcccta ccctgacggg atgtcgcctt tccaggccgc
     4801 catggtagat ggctccggat accaagtcca tcgcacaatg cagtttgaag atggtgcctc
     4861 ccttactgtt aactaccgct acacctacga gggaagccac atcaaaggag aggcccaggt
     4921 gaaggggact ggtttccctg ctgacggtcc tgtgatgacc aactcgctga ccgctgcgga
     4981 ctggtgcagg tcgaagaaga cttaccccaa cgacaaaacc atcatcagta cctttaagtg
     5041 gagttacacc actggaaatg gcaagcgcta ccggagcact gcgcggacca cctacacctt
     5101 tgccaagcca atggcggcta actatctgaa gaaccagccg atgtacgtgt tccgtaagac
     5161 ggagctcaag cactccaaga ccgagctcaa cttcaaggag tggcaaaagg cctttaccga
     5221 tgtgatgggc atggacgagc tgtacaaggg tatagagggc agaggaagtc ttctaacatg
     5281 cggtgacgtg gaggagaatc ccggccctgg cgtcgccgcc accatggtga gcaagggcga
     5341 ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc
     5401 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac
     5461 ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct
     5521 gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga
     5581 ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga
     5641 cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa
     5701 ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat
     5761 gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat
     5821 caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta
     5881 caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat
     5941 cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca
     6001 ctccaccggc ggcatggacg agctgtacaa gacctcaggc aggacaggta gtccaaaaaa
     6061 gaagagaaag gtagatccaa aaaagaagag aaaggtagat ccaaaaaaga agagaaaggt
     6121 attataagcg gcccgagcat cttaccgcca tttattccca tatttgttct gtttttcttg
     6181 atttgggtat acatttaaat gttaataaaa caaaatggtg gggcaatcat ttacattttt
     6241 agggatatgt aattactagt tcaggtgtat tgccacaaga caaacatgtt aagaaacttt
     6301 cccgttattt acgctctgtt cctgttaatc aacctctgga ttacaaaatt tgtgaaagat
     6361 tgactgatat tcttaactat gttgctcctt ttacgctgtg tggatatgct gctttatagc
     6421 ctctgtatct agctattgct tcccgtacgg ctttcgtttt ctcctccttg tataaatcct
     6481 ggttgctgtc tcttttagag gagttgtggc ccgttgtccg tcaacgtggc gtggtgtgct
     6541 ctgtgtttgc tgacgcaacc cccactggct ggggcattgc caccacctgt caactccttt
     6601 ctgggacttt cgctttcccc ctcccgatcg ccacggcaga actcatcgcc gcctgccttg
     6661 cccgctgctg gacaggggct aggttgctgg gcactgataa ttccgtggtg tgtcgaacgc
     6721 gtaccggtta gtaatgacga tggtacctac cgggtagggg aggcgctttt cccaaggcag
     6781 tctggagcat gcgctttagc agccccgctg ggcacttggc gctacacaag tggcctctgg
     6841 cctcgcacac attccacatc caccggtagg cgccaaccgg ctccgttctt tggtggcccc
     6901 ttcgcgccac cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg
     6961 tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc
     7021 accgctgagc aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc
     7081 ttcgctttct gggctcagag gctgggaagg ggtgggtccg ggggcgggct caggggcggg
     7141 ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa
     7201 aagcgcacgt ctgccgcgct gttctcctct tcctcatctc cgggcctttc gactctagac
     7261 acgtgttgac aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag
     7321 gaactaaacc atggccaagc ctttgtctca agaagaatcc accctcattg aaagagcaac
     7381 ggctacaatc aacagcatcc ccatctctga agactacagc gtcgccagcg cagctctctc
     7441 tagcgacggc cgcatcttca ctggtgtcaa tgtatatcat tttactgggg gaccttgtgc
     7501 agaactcgtg gtgctgggca ctgctgctgc tgcggcagct ggcaacctga cttgtatcgt
     7561 cgcgatcgga aatgagaaca ggggcatctt gagcccctgc ggacggtgcc gacaggtgct
     7621 tctcgatctg catcctggga tcaaagccat agtgaaggac agtgatggac agccgacggc
     7681 agttgggatt cgtgaattgc tgccctctgg ttatgtgtgg gagggctaag cacaattcga
     7741 gctcggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     7801 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg
     7861 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     7921 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     7981 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     8041 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
     8101 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca
     8161 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     8221 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc
     8281 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     8341 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     8401 aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg
     8461 cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     8521 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     8581 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc
     8641 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     8701 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     8761 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     8821 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     8881 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     8941 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     9001 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     9061 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     9121 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     9181 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     9241 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     9301 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     9361 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     9421 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     9481 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     9541 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     9601 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     9661 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     9721 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     9781 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     9841 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     9901 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     9961 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
    10021 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
    10081 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
    10141 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
    10201 actgagcgtc agaccccgta gaaaagatca aaggatcttc
//
