LOCUS       sequence-442176-        8817 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tet-inducible shRNA targeting RFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8817)
  AUTHORS   Ilic N, Birsoy K, Aguirre AJ, Kory N, Pacold ME, Singh S, Moody SE, 
            DeAngelo JD, Spardy NA, Freinkman E, Weir BA, Tsherniak A, Cowley 
            GS, Root DE, Asara JM, Vazquez F, Widlund HR, Sabatini DM, Hahn WC
  TITLE     PIK3CA mutant tumors depend on oxoglutarate dehydrogenase.
  JOURNAL   Proc Natl Acad Sci U S A. 2017 Apr 25;114(17):E3434-E3443. doi: 
            10.1073/pnas.1617922114. Epub 2017 Apr 10.
  PUBMED    28396387
REFERENCE   2  (bases 1 to 8817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1617922114"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2017-04-25- 25"; volume: "114"; issue: "17"; pages: 
            "E3434-E3443"
COMMENT     Sequence Label: pLKO-puro-tetON-shRFP
FEATURES             Location/Qualifiers
     source          1..8817
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    184..758
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(352..369)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     579..598
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             778..1377
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(778..797)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1274..1294
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             1505..1738
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1810..1931
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1847..1866)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1901..1920
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1971..2106
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(2127..2145)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2155..2172)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2155..2171)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2164..2186)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2313..2768
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2400..2419)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2610..2631
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2794..2898
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2899..2967,2968..3759)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3117..3136)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3930..4518
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4419..4438
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4672..4689
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4806..4827
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4842..4847,4848..4865,4866..4872)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4880..4896
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4885..4907
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4904..4920
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4904..4920
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4939..4959
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4941..4959
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4987..5213
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             5214..5394
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5441..5566
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6059..6292
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6477..6521
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        6810..6904
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     primer_bind     6821..6840
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    6858..6876
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6886..6904
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    7005..7122
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7171..7681
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     7600..7618
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     intron          7690..8262
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     8270..8289
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        8317..8335
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(8346..8817,1..149)
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(8419..8438)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
ORIGIN
        1 gggaaacacc tactactgat agtatgccgc cattattacg acaagctatc gaattatttg
       61 atcaccaagg tgcagagcca gccttcttat tcggccttga attgatcata tgcggattag
      121 aaaaacaact taaatgtgaa agtgggtccg cgtacagcgg ctcccgggag ttctagggat
      181 ctgcccctct ccctcccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc
      241 ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg
      301 cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca
      361 aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa
      421 gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt
      481 gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt
      541 gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca
      601 acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc
      661 ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc
      721 acggggacgt ggttttcctt tgaaaaacac gatgataagg atccaccgga gcttaccatg
      781 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc
      841 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc
      901 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc
      961 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc
     1021 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc
     1081 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc
     1141 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc
     1201 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag
     1261 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac
     1321 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgacgc
     1381 ccgccccacg acccgcagcg cccgaccgaa aggagcgcac gaccccatgc atcggtacct
     1441 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     1501 ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg
     1561 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     1621 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     1681 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     1741 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag
     1801 agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     1861 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     1921 aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc
     1981 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     2041 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     2101 gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc
     2161 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     2221 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     2281 ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc
     2341 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     2401 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     2461 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     2521 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     2581 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     2641 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     2701 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct
     2761 tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     2821 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     2881 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     2941 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     3001 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     3061 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     3121 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     3181 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     3241 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     3301 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     3361 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     3421 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     3481 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     3541 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     3601 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     3661 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     3721 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     3781 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     3841 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     3901 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     3961 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     4021 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     4081 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     4141 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     4201 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     4261 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     4321 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     4381 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     4441 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     4501 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     4561 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     4621 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     4681 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     4741 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     4801 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     4861 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
     4921 catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa
     4981 gcttaatgta gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc
     5041 aacatgcctt acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt
     5101 acgatcgtgc cttattagga aggcaacaga cgggtctgac atggattgga cgaaccactg
     5161 aattgccgca ttgcagagat attgtattta agtgcctagc tcgatacata aacgggtctc
     5221 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     5281 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     5341 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
     5401 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
     5461 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
     5521 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
     5581 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
     5641 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
     5701 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
     5761 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
     5821 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
     5881 aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga
     5941 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
     6001 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
     6061 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
     6121 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
     6181 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
     6241 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
     6301 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
     6361 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
     6421 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
     6481 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
     6541 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
     6601 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
     6661 cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag
     6721 aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc
     6781 gatcacgaga ctagcctcga gcggccgcaa tatttgcatg tcgctatgtg ttctgggaaa
     6841 tcaccataaa cgtgaaatcc ctatcagtga tagagactta taagttccct atcagtgata
     6901 gagacaccgg tcagttccag tacggctcca actcgagttg gagccgtact ggaactgatt
     6961 tttgaattct cgacctcgag acaaatggca gtattcatcc acaattttaa aagaaaaggg
     7021 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
     7081 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac
     7141 agcagagatc cactttggcc gcggctcgag ggggttgggg ttgcgccttt tccaaggcag
     7201 ccctgggttt gcgcagggac gcggctgctc tgggcgtggt tccgggaaac gcagcggcgc
     7261 cgaccctggg tctcgcacat tcttcacgtc cgttcgcagc gtcacccgga tcttcgccgc
     7321 tacccttgtg ggccccccgg cgacgcttcc tgctccgccc ctaagtcggg aaggttcctt
     7381 gcggttcgcg gcgtgccgga cgtgacaaac ggaagccgca cgtctcacta gtaccctcgc
     7441 agacggacag cgccagggag caatggcagc gcgccgaccg cgatgggctg tggccaatag
     7501 cggctgctca gcagggcgcg ccgagagcag cggccgggaa ggggcggtgc gggaggcggg
     7561 gtgtggggcg gtagtgtggg ccctgttcct gcccgcgcgg tgttccgcat tctgcaagcc
     7621 tccggagcgc acgtcggcag tcggctccct cgttgaccga atcaccgacc tctctcccca
     7681 gggggatctg tgagtttggg gacccttgat tgttctttct ttttcgctat tgtaaaattc
     7741 atgttatatg gagggggcaa agttttcagg gtgttgttta gaatgggaag atgtcccttg
     7801 tatcaccatg gaccctcatg ataattttgt ttctttcact ttctactctg ttgacaacca
     7861 ttgtctcctc ttattttctt ttcattttct gtaacttttt cgttaaactt tagcttgcat
     7921 ttgtaacgaa tttttaaatt cacttttgtt tatttgtcag attgtaagta ctttctctaa
     7981 tcactttttt ttcaaggcaa tcagggtata ttatattgta cttcagcaca gttttagaga
     8041 acaattgtta taattaaatg ataaggtaga atatttctgc atataaattc tggctggcgt
     8101 ggaaatattc ttattggtag aaacaactac atcctggtca tcatcctgcc tttctcttta
     8161 tggttacaat gatatacact gtttgagatg aggataaaat actctgagtc caaaccgggc
     8221 ccctctgcta accatgttca tgccttcttc tttttcctac agctcctggg caacgtgctg
     8281 gttattgtgc tgtctcatca ttttggcaaa gaattgtaat acgactcact atagggcgaa
     8341 ttgatatgtc tagattagat aaaagtaaag tgattaacag cgcattagag ctgcttaatg
     8401 aggtcggaat cgaaggttta acaacccgta aactcgccca gaagctaggt gtagagcagc
     8461 ctacattgta ttggcatgta aaaaataagc gggctttgct cgacgcctta gccattgaga
     8521 tgttagatag gcaccatact cacttttgcc ctttagaagg ggaaagctgg caagattttt
     8581 tacgtaataa cgctaaaagt tttagatgtg ctttactaag tcatcgcgat ggagcaaaag
     8641 tacatttagg tacacggcct acagaaaaac agtatgaaac tctcgaaaat caattagcct
     8701 ttttatgcca acaaggtttt tcactagaga atgcattata tgcactcagc gctgtggggc
     8761 attttacttt aggttgcgta ttggaagatc aagagcatca agtcgctaaa gaagaaa
//
