LOCUS       sequence-442191-        6860 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor plasmid of hAT-8_PM Transposon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6860)
  AUTHORS   Zhang T, Tan S, Tang N, Li Y, Zhang C, Sun J, Guo Y, Gao H, Cai Y, 
            Sun W, Wang C, Fu L, Ma H, Wu Y, Hu X, Zhang X, Gee P, Yan W, Zhao 
            Y, Chen Q, Guo B, Wang H, Zhang YE
  TITLE     Heterologous survey of 130 DNA transposons in human cells highlights
            their functional divergence and expands the genome engineering 
            toolbox.
  JOURNAL   Cell. 2024 Jul 11;187(14):3741-3760.e30. doi: 
            10.1016/j.cell.2024.05.007. Epub 2024 Jun 5.
  PUBMED    38843831
REFERENCE   2  (bases 1 to 6860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2024.05.007"; journalName: "Cell"; date: 
            "2024-07-11- 11"; volume: "187"; issue: "14"; pages: "3741-3760.e30"
COMMENT     Sequence Label: pMV-hAT-8_PM
FEATURES             Location/Qualifiers
     source          1..6860
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     94..112
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     121..138
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     122..138
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2580..3079
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2615..2638)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3012..3031
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      3093..3102
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3099..3698
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MGTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     3598..3618
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             3705..3761
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(3762..3764,3765..3767,3768..4481)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3762..3764,3765..3767,3768..4478)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3807..3828)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4068..4087)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4415..4436
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4503..4520)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4509..4733
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4963..4979)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4963..4979)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4991..5008)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5162..5750)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5242..5261)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5921..6712,6713..6781))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLRNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERKRQIAEIGAS
                     LIKHW"
     primer_bind     6544..6563
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(6782..6860,1..26))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
       61 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
      121 tgtaaaacga cggccagtcg aaccacgcaa tgcgtctcga tccgcagtgt cttgcgtctc
      181 taagcttctc tacagcaggg gtctcaaact cgcggcccgc gggccaattg cggcccgcgg
      241 aacgatattt tgtggccccc tacttgacat caattttttt tttaattatt attttttttt
      301 accatagctc aggctcgtga cagcttccgg tgtttgttga caagttagcc aacgcgaatt
      361 agcaacgtta gtcacttggt tgaaacgtga ttccggagtg acaccaagtg gaaggaaaat
      421 atatcctgtc acccagtgca aggtacagag gacttaggaa gtgggcggac tcaacaagag
      481 taatcagtga cctaataaag acaacagagt gttgcaatag gaaagggaaa ataattacat
      541 ggatagcaaa tagcatgaga tgggtggcaa gaacaaaagc caaatggtgg ttagaacctg
      601 aatattataa agaagaagtt atagattatt attacaagag aaacaaagac aaggaaaaag
      661 gacagatatg tgcaatcgca aaagaaaacg aatttaccaa gaacaattta atcaaaaaga
      721 gcaagaaatt aaaagatata aaacatgaga caatgcactt gatgttaaga attaagacag
      781 gtggagtaac atggacacca gagttagctc aatggcatcc agagagattt ggtagagcag
      841 aaggatatgt aggatgtaaa caaaaagaag aaaatctaga tcactttatt tatgaatgtg
      901 aaaggtataa acacctgaga gagaacacga ttgataaatt taataatgat aagaaaaacg
      961 aagtgctcaa aaaaagctat gaacaatgat aaagttttca aatacctctc agaatatata
     1021 gaaagggcat actaaataag atcccaaata ctcaggtatt gggataaaaa catgtaaata
     1081 tatcacaatg gaaagcccgc cttaagggtg accaaagcgc tgtaaaagtg tatggtaggc
     1141 agaagccaaa gcattgatag taccgtaatt tccggactat tgagcgcacc tgattataag
     1201 ccgcacccac taactttaaa aagaaagttg tacatatata ggccgcattt gtctataagc
     1261 cgcaggtgtc cacgttgtaa catgagatat ttacacggaa agattttttt aacttttaat
     1321 taaatagtac cggtaacata cacaaatacg tactgtaaat gcttttttcc cgaacagtag
     1381 ccaaccagga acagtccatc cattttctat ccccgctttg tcctaatcag ggtcgcgggg
     1441 gggggggctg cagcctatcc catctgcttt tttttacctc tgaaagcttt tgtcgtcttt
     1501 tttcattgag tcagttcttc acgctgccgt ctccaacgtc tcaccattga ttcattgatg
     1561 ccaagcttac gtgcagcagc tctatttcct tctttgacag ccagatcgac cgccttttaa
     1621 cttgaaagct gcatcatatg cattccttcg cgtgttttcc atgatgacgg tgtgtgcatg
     1681 aagcgcaaaa tgactgatct gaagaattta gtgtgagtgc atttgattgg tccactgtgg
     1741 cctgttcggt aatttaattg gtctgatgtg acggtaaatg ttttggcggc atgaagcttg
     1801 tgtaaaaaaa acataaatta gccgcatcat tgtttaagcc gcagtgttca aagcgtgtaa
     1861 aaaaagtagc ggcttatagt ccggaaattt acggtagttt tttttattaa ctttttttct
     1921 attattatta aatgtgtttt gttttttttt gtcacccagc cccagtcatg tctttctcaa
     1981 aacctgccgt gaagagaaag gttggcgacg agcacagaca atttcaggaa aagtgggaga
     2041 cggaatattt tttttgttga gcacaggggc accccgacgt gtcttatatg cacagagaaa
     2101 gttgcggtgc acaaggaata caacatcaga cgtcattact taactagaca tactgaggag
     2161 tatgcaaaat accagggaga tgagagagag aggaccgggt cgccaatctt aaaacatgtc
     2221 tactgaggca acaagatttt ttcaagaaag caagcaaaga gagtgatgca gcagtcgaac
     2281 tagctacgtg gtgagtgaga tgattgctaa ggcgggaaag ccattcaaag acggcgagtt
     2341 catcaaaaag tgcatgttac aggctgcaag tatagtctgt ccggaaaaga agggtcagtt
     2401 tagcaacatc agcctttcag ccaacacagt ggcagagcgc atttctgacc tgtcaggtaa
     2461 catatacgat caactgcgtg agaaagccaa cacatttccg tgcatactca gtcgctcttg
     2521 atgagagcac agacatcact gacactgcgc agctcgcaac cggtgctagc aattctaccg
     2581 ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg
     2641 cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg
     2701 ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag
     2761 gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg
     2821 tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg
     2881 gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg
     2941 tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc
     3001 cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc
     3061 ctcatctccg ggcctttcga cctgcagcca acgccaccat ggggaccgag tacaagccca
     3121 cggtgcgcct cgccacccgc gacgacgtcc cccgggccgt acgcaccctc gccgccgcgt
     3181 tcgccgacta ccccgccacg cgccacaccg tcgacccgga ccgccacatc gagcgggtca
     3241 ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg
     3301 cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg
     3361 tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc
     3421 aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca
     3481 ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg
     3541 gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca
     3601 acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag
     3661 gaccgcgcac ctggtgcatg acccgcaagc ccggtgccat cgatgccact aacttctccc
     3721 tgttgaaaca agcaggggat gtcgaagaga atcccgggcc aatggtgagc aagggcgagg
     3781 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     3841 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
     3901 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
     3961 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     4021 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     4081 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     4141 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     4201 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
     4261 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     4321 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg
     4381 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     4441 ccgccgggat cactctcggc atggacgagc tgtacaagta attaattaag ctcgctgatc
     4501 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     4561 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     4621 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     4681 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
     4741 ggcgcgccct cgaggtaaga gaagcctatg ttctgaagaa ccgttcatgt tctgaactgt
     4801 tattaataaa gattgagaag attggatcag ttgtactttt ttttgaatac ttgatgcggc
     4861 ccagcctcac ccagactcta cctccagcgg cccccaggta agttgagttt gagacccctg
     4921 ctctacaggg gcccagagac ggagtcactg ccaaccgaga cggtcatagc tgtttcctgt
     4981 gtgccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     5041 cagctcactc aaaggcggta atacggttac ccacagaatc aggggataac gcaggaaaga
     5101 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     5161 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     5221 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     5281 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     5341 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     5401 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     5461 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     5521 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     5581 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     5641 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     5701 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     5761 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     5821 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     5881 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     5941 aggcacctat ctcagcgatc tgtctctttc gttcatccat agttgcctga ctccccgtcg
     6001 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca ataataccgc
     6061 gggacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     6121 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     6181 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc atcgctacag
     6241 gcatcgtggt atcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     6301 caaggcgagt tacatgatcc cccatgttgc gcaaaaaagc ggttagctcc ttcggtcctc
     6361 cgatcgttgt cagaagtaag ttggccgccg tgttatcact catggttatg gcagcactac
     6421 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     6481 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     6541 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     6601 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     6661 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     6721 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     6781 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     6841 acatatttga atgtatttag
//
