LOCUS       sequence-442249-        2520 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To express MelG (Rv1937) in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Panagoda NT, Balazsi G, Sampson NS
  TITLE     Mycobacterium tuberculosis Mce3R TetR-like Repressor Forms an 
            Asymmetric Four-Helix Bundle and Binds a Nonpalindrome 
            Sequencedagger.
  JOURNAL   ACS Chem Biol. 2024 Dec 20;19(12):2580-2592. doi: 
            10.1021/acschembio.4c00687. Epub 2024 Nov 15.
  PUBMED    39545866
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acschembio.4c00687"; journalName: "ACS Chem Biol"; date: 
            "2024-12-20- 20"; volume: "19"; issue: "12"; pages: "2580-2592"
COMMENT     Sequence Label: pET28b-MelG
FEATURES             Location/Qualifiers
     source          1..2520
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggcggttc gtcaggtcac cgtcggctat tcggacggca cgcacaagac gatgccggtg
       61 cggtgcgacc agacggtcct ggatgccgcc gaggaacacg gcgtggccat cgtcaacgaa
      121 tgccaaagcg ggatatgtgg cacctgcgtg gccacctgca ccgccggccg ctaccagatg
      181 ggacgcaccg agggactgtc cgatgtcgag cgggcggcgc gaaagatcct cacctgccag
      241 acgtttgtta cctccgattg ccggatcgag ctgcagtatc cggtcgacga caacgccgcc
      301 ctgctggtca ccggtgacgg tgtggtgacc gcggtcgagt tggtgtcgcc cagcaccgcc
      361 atcctgcggg tggacacctc tggcatggcc ggcgcgctga gataccgggc cggccagttc
      421 gcccaattgc aggttcccgg taccaacgta tggcgcaact actcctacgc ccatccggcc
      481 gacggccgcg gtgagtgcga gttcatcatc aggttgctgc cggacggcgt gatgtcgaat
      541 tatcttcgcg accgcgccca gcccggtgac catatcgcgc tgcgctgcag caagggcagc
      601 ttttatctgc gcccgatcgt gcgaccggtg atcctggtcg ccggaggaac cggcctgtca
      661 gcgatcctgg cgatggccca gagcctggat gccgatgtcg ctcacccggt ctacctgctc
      721 tacggggtcg agcgcaccga agacctgtgc aagctcgacg aactcaccga gctgcgccgc
      781 cgcgttggcc gcctggaggt gcacgtcgtc gtcgctcgcc cggaccccga ctgggatggg
      841 cgcaccgggc tggtcaccga cctgctcgac gagcggatgc tggcgagcgg tgacgccgac
      901 gtgtatctgt gcggtccggt cgccatggtc gacgcagccc gaacctggct ggaccacaat
      961 ggctttcacc gtgtcgggtt gtactacgag aagttcgtgg ccagcggggc ggcgcgccgc
     1021 cgcaccccgg ctcggctgga ttacgcgggc gtggacattg ccgaggtgtg ccgccgcggc
     1081 cgcggcaccg cggtggtcat cggcggcagc atcgcgggca tcgcggcggc gaaaatgctc
     1141 agcgagacct tcgatcgcgt catcgtgctg gagaaggacg gcccgcaccg tcgccgcgag
     1201 ggcaggccgg gcgcggcaca gggttggcac ctgcaccacc tgctgaccgc cgggcagatc
     1261 gagctggagc gcatcttccc tggcatcgtc gacgacatgg tgcgcgaggg agcgttcaag
     1321 gtcgacatgg ccgcgcagta ccgtatccgg ctgggcggca cctggaagaa gcccggcact
     1381 agtgacatcg agatcgtctg cgcgggaagg ccgctgctcg aatggtgtgt gcgccgccgg
     1441 ctcgacgacg aaccgcgcat cgacttccgc tacgaatcgg aggtggccga tctcgccttc
     1501 gaccgcgcca acaatgccat cgtcggcgtc gccgtggaca atggcgacgc cgacggaggc
     1561 gacggtttgc aggtggtgcc cgccgagttc gtcgtggacg cgtcgggcaa gaacacccgc
     1621 gtgccggagt tcttggagcg tctcggtgtt ggcgctcccg aggccgagca ggacatcatc
     1681 aactgcttct actccacgat gcagcaccgg gttccgccgg agcggcggtg gcaggacaag
     1741 gtgatggtga tctgctatgc gtaccgccct ttcgaggata cctacgccgc gcagtactac
     1801 accgacagct cccgcaccat cctgtccacc tcactggtgg cctacaactg ctattcgccg
     1861 ccgcgtaccg cccgagaatt ccgcgcgttc gccgacctga tgccgtcccc ggtcatcggg
     1921 gagaacatcg acgggctgga gccggcatcg cccatctaca atttccgcta tcccaacatg
     1981 ctgcggctgc gctacgagaa gaagcgcaac ctgccgcggg ctttgctggc ggtgggcgat
     2041 gcctacacca gcgccgaccc ggtgtcgggt ctgggtatga gcctggcgct caaggaagtt
     2101 cgggagatgc aggcgctgct ggctaaatac ggcgccggtc accgggatct gccgcgccgg
     2161 tactaccggg cgatcgccaa gatggccgac acggcctggt tcgtgatccg cgagcagaac
     2221 ctgcgcttcg actggatgaa ggacgtcgac aagaagcgcc cgttctattt cggtgtgctg
     2281 acctggtaca tggaccgcgt gctggagctg gtgcatgacg atctcgacgc gtaccgggaa
     2341 ttcttggccg tcgtccatct ggtcaagccg ccgtcggcgc tgatgcgacc caggatcgcc
     2401 agccgcgtcc tcggcaaatg ggcacgaacc cgattgtcgg gccagaagac gttgattgcc
     2461 cgcaactacg aaaatcatcc gataccagcc gaacccgcgg accaacttgt aaacgcttag
//
