LOCUS       sequence-442253-         273 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To express VapBC35(Rv1962) Toxin antitoxin in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 273)
  AUTHORS   Panagoda NT, Balazsi G, Sampson NS
  TITLE     Mycobacterium tuberculosis Mce3R TetR-like Repressor Forms an 
            Asymmetric Four-Helix Bundle and Binds a Nonpalindrome 
            Sequencedagger.
  JOURNAL   ACS Chem Biol. 2024 Dec 20;19(12):2580-2592. doi: 
            10.1021/acschembio.4c00687. Epub 2024 Nov 15.
  PUBMED    39545866
REFERENCE   2  (bases 1 to 273)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acschembio.4c00687"; journalName: "ACS Chem Biol"; date: 
            "2024-12-20- 20"; volume: "19"; issue: "12"; pages: "2580-2592"
COMMENT     Sequence Label: pET28b-VapBC35
FEATURES             Location/Qualifiers
     source          1..273
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gtgaatgagg tgtccatacg aacgctcaac caggagacgt ccaaggtcct ggcccgcgtc
       61 aagcgcggtg aagagatcaa cctgactgag cgcggcaagg ttatcgcccg aataatcccg
      121 gcttctgccg gccctctcga ctcactgatc agcaccggca gtgtgcaacc ggcgagagtg
      181 catggcccgg cgcctcggcc cacaattccg atgcgcggcg gtctcgactc gggaacgctg
      241 ttggagcgca tgcgcgccga ggagcggtac tag
//
