LOCUS       sequence-442262-        2482 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To express igr3 (Rv3542c-Rv3541c-Rv3540c) with K253E mutation in 
            Rv3542c in Mycobacteria .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Panagoda NT, Balazsi G, Sampson NS
  TITLE     Mycobacterium tuberculosis Mce3R TetR-like Repressor Forms an 
            Asymmetric Four-Helix Bundle and Binds a Nonpalindrome 
            Sequencedagger.
  JOURNAL   ACS Chem Biol. 2024 Dec 20;19(12):2580-2592. doi: 
            10.1021/acschembio.4c00687. Epub 2024 Nov 15.
  PUBMED    39545866
REFERENCE   2  (bases 1 to 2482)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acschembio.4c00687"; journalName: "ACS Chem Biol"; date: 
            "2024-12-20- 20"; volume: "19"; issue: "12"; pages: "2580-2592"
COMMENT     Sequence Label: pMV261-igr3K253E
FEATURES             Location/Qualifiers
     source          1..2482
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gtgaccgggg tgagcgacat tcaggaagcg gttgcccaga tcaaggcggc cgggcccagc
       61 aagccgcgcc tggcccgtga cccggtgaac cagccgatga tcaacaactg ggtggaggcc
      121 atcggcgacc gcaaccccat ctatgtggac gacgcggcgg ctcgcgccgc cggccacccc
      181 ggaatcgtcg cgccaccggc catgattcag gtgtggacca tgatgggtct gggcggagtg
      241 cgtccgaagg acgatccgct gggacccatc atcaagctgt tcgacgacgc cggctacatc
      301 ggtgtggttg ccaccaactg cgagcagacc taccaccgct acctattgcc cggggagcaa
      361 gtcagcatca gcgccgagct cggtgacgtc gtcggaccca agcaaaccgc gctcggcgag
      421 ggctggttca tcaaccagca catcgtatgg caagtcgggg acgaggacgt cgcagagatg
      481 aattggcgca tcctcaaatt caagcccgcg ggttctccga gctcggtgcc tgacgatttg
      541 gatcccgacg ctatgatgcg tccctcgtcg tcgcgggaca ccgcgttttt ctgggatgga
      601 gtcaaggccc acgaattgcg gatccagcgg cttgccgatg gcagcctgcg gcacccaccg
      661 gtgccggcgg tgtggcaaga caaatcggtg ccgatcaact acgtcgtctc cagcggtcgc
      721 ggcacggtgt tcagcttcgt ggtgcatcac gcgccggagg ttccgggtcg cacggtgccc
      781 ttcgtgatcg cgctggtgga gctcgaggaa ggggtacgca tgctgggcga gctgcggggg
      841 gccgatcctg ctcgtgttgc gatcggtatg ccggtacgag cgacctatat cgatttcccc
      901 gactggagcc tctacgcatg ggagcctgac gaatgacggt tgttggtgct gtgctgcctg
      961 agctgaagct ctacggagac ccgacgttca tcgtctcaac ggcgctggct accagggact
     1021 tccaggatgt gcatcatgat cgagacaagg ccgtcgccca ggggtcgaaa gacattttcg
     1081 tcaacatcct caccgacacc gggctggtgc aacgctacgt caccgactgg gcgggcccgt
     1141 cggcgctgat caagtcgatt gggctgcggc tgggggtgcc gtggtacgcc tacgacacgg
     1201 tgacgttctc cggtgaggtg accgcggtga acgacggctt gatcacggta aaggtggtgg
     1261 gccgcaatac ccttggcgac cacgtgaccg cgacggtgga actttcgatg agggattcgt
     1321 agtgttatcg ggtcaggcgg ccatcgtcgg tatcggcgcc accgactttt cgaagaactc
     1381 cggtcgaagt gagctgcggc tggcggccga ggcggtgttg gatgcgttgg ccgatgcggg
     1441 cctgagcccg accgatgtcg acgggctgac cacgttcacg atggacacca acaccgaaat
     1501 cgccgtggcg cgtgcggccg gcatcggcga gctgacgttc ttctccaaga tccactacgg
     1561 cggtggcgcc gcatgtgcga ccgtgcagca cgccgctatg gcagtggcca ccggggtggc
     1621 tgacgtcgtg gtggcgtatc gggcattcaa cgaacgatcc ggcatgcggt tcggtcaggt
     1681 gcaaactcgt ttgaccgaga atgccgactc caccggcgtg gacaattcgt tttcgtatcc
     1741 gcacgggctc tccacgcccg ccgcgcaagt ggcgatgatc gctcgccggt acatgcacct
     1801 gtctggtgcg accagccggg acttcggtgc tgtctcggtg gccgaccgca agcatgccgc
     1861 caacaacccc aaggcgtact tctacggcaa gccgataacc attgaggacc accagaattc
     1921 gaggtggatc gccgagccgc tgcggctgct ggactgctgc caggagaccg acggcgcggt
     1981 cgcgatcgtg gtgacgtcag ctgcgcgcgc acgggacctc aagcagcgcc cggtggtcat
     2041 tgaggcggct gcgcagggct gcagtccaga ccagtacacg atggtcagct actaccggcc
     2101 ggaactcgac ggcctgcccg agatgggcct ggtgggccgg cagctatggg cgcagtcggg
     2161 gctgacgccg gccgatgtcc agaccgcagt cctctacgac cacttcacgc cgtttaccct
     2221 gattcagttg gaggagttgg gattctgcgg caagggcgaa gcgaaagact tcatcgccga
     2281 cggcgcgatc gaggtgggcg ggcggctgcc catcaacacc cacggcggtc aactcggcga
     2341 agcctacatc catggcatga acggcatcgc ggagggggtg cggcagctgc gcggcacctc
     2401 ggtgaacccg gtggcgggcg tcgagcatgt gctcgtcacc gcgggcaccg gggtgcctac
     2461 gtccgggctg atcctgggtt aa
//
