LOCUS       sequence-442266-        1032 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To express TrxB2(Rv3913) in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Panagoda NT, Balazsi G, Sampson NS
  TITLE     Mycobacterium tuberculosis Mce3R TetR-like Repressor Forms an 
            Asymmetric Four-Helix Bundle and Binds a Nonpalindrome 
            Sequencedagger.
  JOURNAL   ACS Chem Biol. 2024 Dec 20;19(12):2580-2592. doi: 
            10.1021/acschembio.4c00687. Epub 2024 Nov 15.
  PUBMED    39545866
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acschembio.4c00687"; journalName: "ACS Chem Biol"; date: 
            "2024-12-20- 20"; volume: "19"; issue: "12"; pages: "2580-2592"
COMMENT     Sequence Label: pET28a(+)-TrxB2
FEATURES             Location/Qualifiers
     source          1..1032
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..24
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 ctagaagtat tatttcaagg acccatgaca gctccgcctg ttcatgatag agcgcatcat
       61 ccggttcgtg acgtgattgt tattggctcc ggtccggcag gctacaccgc ggcgctgtat
      121 gcggcgcgtg cgcaactggc gccgcttgtg ttcgagggca cctcgttcgg cggtgctctg
      181 atgacgacca ctgacgtgga aaactacccg ggctttcgta atggcattac cggaccggaa
      241 ctgatggatg agatgcgtga acaggctctt cgctttggtg ctgacctgcg catggaagat
      301 gttgagagcg tctccctgca tggtccgctg aaaagcgtag ttactgcgga cggtcagacc
      361 caccgtgcta gggcggtgat cctggctatg ggtgcggcgg cgcgttatct gcaggttccg
      421 ggtgaacaag aactgctggg tcgtggagtc agcagctgcg cgacgtgcga tggtttcttc
      481 tttcgtgacc aggatattgc cgtaatcggc ggcggcgata gcgcaatgga ggaagcaacc
      541 tttctgaccc gtttcgccag aagcgtgacc ttagtgcacc gccgtgacga gttccgtgct
      601 tccaagatca tgctggatcg tgctcgcaac aacgataaaa tccgcttttt gacgaaccac
      661 accgttgttg cagttgacgg cgacaccacc gtgacgggct tgcgcgtgcg tgataccaat
      721 accggtgcag agacaacgct gccagtcacg ggcgtttttg ttgccatcgg ccacgaaccg
      781 cgtagtggtt tggtgcgcga ggccatcgac gtggacccgg atggctacgt gctcgtccag
      841 ggtcgcacca cctctactag cttgccgggc gtgttcgcgg ccggtgactt ggttgaccgt
      901 acctatcgtc aagctgttac cgcagcgggt tctggttgtg cggcggcaat cgatgcggaa
      961 cgttggctgg cagagcacgc ggccaccggt gaggcggaca gcaccgacgc actgattggt
     1021 gctcaacgtt aa
//
