LOCUS       sequence-442283-       11585 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  An SspB-tagged maltose binding protein (MBP) with an infrared 
            fluorescent protein for visualization. Used to demonstrate 
            condensate dissolution using the OptoMBP system..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11585)
  AUTHORS   Brumbaugh-Reed EH, Aoki K, Toettcher JE
  TITLE     Rapid and reversible dissolution of biomolecular condensates using 
            light-controlled recruitment of a solubility tag
  JOURNAL   bioRxiv 2024.01.16.575860
REFERENCE   2  (bases 1 to 11585)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.01.16.575860"
COMMENT     Sequence Label: pHR myc-MBP-SspB-iRFP-NLS
FEATURES             Location/Qualifiers
     source          1..11585
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             223..267
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    552..669
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        822..1229
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      1295..1304
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1304..1333
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1346..2443
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="KIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEE
                     KFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLI
                     AYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAA
                     DGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGET
                     AMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLE
                     NYLLTDEGLEAVNKDKPLGAVALKSYEEELVKDPRIAATMENAQKGEIMPNIPQMSAFW
                     YAVRTAVINAASGRQTVDEALKDAQT"
     primer_bind     2417..2440
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             2519..2848
                     /codon_start=1
                     /product="SspB from Haemophilus influenzae, with the 
                     mutations Y11K and A15E to disrupt the natural dimerization
                     of the protein"
                     /label=SspB nano
                     /note="dimerizes with iLID at high affinity to create iLID 
                     nano (Guntas et al., 2015)"
                     /translation="SSPKRPKLLREYYDWLVDNSFTPYLVVDATYLGVNVPVEYVKDGQ
                     IVLNLSASATGNLQLTNDFIQFNARFKGVSRELYIPMGAALAIYARENGDGVMFEPEEI
                     YDELNI"
     CDS             2852..3811
                     /codon_start=1
                     /product="naturally monomeric infrared fluorescent protein 
                     derived from a truncated bacteriophytochrome (Yu et al., 
                     2015)"
                     /label=mIFP
                     /translation="MSVPLTTSAFGHAFLANCEREQIHLAGSIQPHGILLAVKEPDNVV
                     IQASINAAEFLNTNSVVGRPLRDLGGDLPLQILPHLNGPLHLAPMTLRCTVGSPPRRVD
                     CTIHRPSNGGLIVELEPATKTTNIAPALDGAFHRITSSSSLMGLCDETATIIREITGYD
                     RVMVVRFDEEGNGEILSERRRADLEAFLGNRYPASTIPQIARRLYEHNRVRLLVDVNYT
                     PVPLQPRISPLNGRDLDMSLSCLRSMSPIHQKYMQDMGVGATLVCSLMVSGRLWGLIAC
                     HHYEPRFVPFHIRAAGEALAETCAIRIATLESFAQSQSK"
     CDS             3824..3850
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     misc_feature    3951..4539
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4004..4024)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4422..4433)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4542..4558)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4543..4559)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4649..4882
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(4907..4925)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4908..4925)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5215..5234)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5448..5467)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5567..5589
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5627..5645)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5713..5817
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5818..5886,5887..6678)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6036..6055)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6849..7437
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7338..7357
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7591..7608
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7678..7698)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7683..8012
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7863..7998
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          9146..9211
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9341..9361
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9786..9920
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9823..9842)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9877..9896
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             10088..10721
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10768..10893
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    join(11390..11585,1..38)
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga
       61 aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa
      121 cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc
      181 ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta
      241 ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg
      301 tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct
      361 gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac
      421 ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga
      481 gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcca aatggcagta
      541 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
      601 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
      661 caaaattttc gggtttatta cagggacagc agagatccag tttggatcga taagcttgat
      721 atcgaattcc tgcagccccg ataaaataaa agattttatt tagtctccag aaaaaggggg
      781 gaatgaaaga ccccacctgt aggtttggca agctagctgc agtaacgcca ttttgcaagg
      841 catggaaaaa taccaaacca agaatagaga agttcagatc aagggcgggt acatgaaaat
      901 agctaacgtt gggccaaaca ggatatctgc ggtgagcagt ttcggccccg gcccggggcc
      961 aagaacagat ggtcaccgca gtttcggccc cggcccgagg ccaagaacag atggtcccca
     1021 gatatggccc aaccctcagc agtttcttaa gacccatcag atgtttccag gctcccccaa
     1081 ggacctgaaa tgaccctgcg ccttatttga attaaccaat cagcctgctt ctcgcttctg
     1141 ttcgcgcgct tctgcttccc gagctctata aaagagctca caacccctca ctcggcgcgc
     1201 cagtcctccg acagactgag tcgcccgggg gggatctgga gctctcgaga attctcacgc
     1261 gtcaagtgga gcaaggcagg tggacagtgg atccgccacc atggaacaaa aactcatctc
     1321 agaagaggat ctgggaggag ggtctaaaat cgaagaaggt aaactggtaa tctggattaa
     1381 cggcgataaa ggctataacg gtctcgctga agtcggtaag aaattcgaga aagataccgg
     1441 aattaaagtc accgttgagc atccggataa actggaagag aaattcccac aggttgcggc
     1501 aactggcgat ggccctgaca ttatcttctg ggcacacgac cgctttggtg gctacgctca
     1561 atctggcctg ttggctgaaa tcaccccgga caaagcgttc caggacaagc tgtatccgtt
     1621 tacctgggat gccgtacgtt acaacggcaa gctgattgct tacccgatcg ctgttgaagc
     1681 gttatcgctg atttataaca aagatctgct gccgaacccg ccaaaaacct gggaagagat
     1741 cccggcgctg gataaagaac tgaaagcgaa aggtaagagc gcgctgatgt tcaacctgca
     1801 agaaccgtac ttcacctggc cgctgattgc tgctgacggg ggttatgcgt tcaagtatga
     1861 aaacggcaag tacgacatta aagacgtggg cgtggataac gctggcgcga aagcgggtct
     1921 gaccttcctg gttgacctga ttaaaaacaa acacatgaat gcagacaccg attactccat
     1981 cgcagaagct gcctttaata aaggcgaaac agcgatgacc atcaacggcc cgtgggcatg
     2041 gtccaacatc gacaccagca aagtgaatta tggtgtaacg gtactgccga ccttcaaggg
     2101 tcaaccatcc aaaccgttcg ttggcgtgct gagcgcaggt attaacgccg ccagtccgaa
     2161 caaagagctg gcaaaagagt tcctcgaaaa ctatctgctg actgatgaag gtctggaagc
     2221 ggttaataaa gacaaaccgc tgggtgccgt agcgctgaag tcttacgagg aagagttggt
     2281 gaaagatccg cgtattgccg ccactatgga aaacgcccag aaaggtgaaa tcatgccgaa
     2341 catcccgcag atgtccgctt tctggtatgc cgtgcgtact gcggtgatca acgccgccag
     2401 cggtcgtcag actgtcgatg aagccctgaa agacgcgcag acttccggac tcagatctcg
     2461 agctcaagct tcgaacgaat tcggcattga tctgagcggc ctgaccctgc aggaattcag
     2521 ctccccgaaa cgccctaagc tgctgcgtga atattacgat tggctggttg ataacagctt
     2581 taccccatat ctggtggtgg atgccacata cctgggcgtg aacgtgcccg tggagtatgt
     2641 gaaagacggt cagatcgtgc tgaatctgtc tgcaagtgcg accggcaacc tgcaactgac
     2701 aaatgatttt atccagttca acgcccgctt taagggcgtg tctcgtgaac tgtatatccc
     2761 gatgggtgcc gctctggcca tttacgctcg cgagaacggc gatggtgtga tgttcgaacc
     2821 agaagaaatc tatgacgagc tgaatattgg tatgtcggta ccgctgacta cctcagcatt
     2881 cggccacgcg tttctggcta actgtgaacg cgagcagatc cacctggcgg gctccattca
     2941 gccgcacggt atcctgctgg ctgtgaaaga gccggacaac gtggtgatcc aggcttctat
     3001 taacgctgcg gagttcctga acaccaactc tgttgttggc cgtccgctgc gtgacctggg
     3061 cggcgatctg cctttgcaga tcctgccgca cctgaacggc ccgctgcacc tggctccgat
     3121 gaccctgcgt tgtaccgtgg gttctccgcc gcgtcgtgtg gactgtacca ttcatcgtcc
     3181 gtctaacggc ggcctgatcg tagaactgga accagcaacc aagaccacta acattgcgcc
     3241 ggctctggac ggtgcgtttc atcgtatcac ttcttcatcc tccctgatgg gcctgtgtga
     3301 cgaaaccgcg actattatcc gtgagattac tggctacgac cgtgtgatgg tagtacgttt
     3361 cgatgaagag ggtaatggcg aaattctgtc cgaacgtcgt cgtgcggacc tggaagcgtt
     3421 cctgggtaac cgctacccgg cgtctactat tccgcagatc gctcgtcgcc tgtacgaaca
     3481 taaccgtgtt cgcctgctgg tagatgtgaa ctatactccg gttccgctac agccgcgcat
     3541 cagcccgctg aacggtcgtg atctggatat gtccctgtct tgcctgcgct ctatgtcccc
     3601 gatccaccag aaatacatgc aggacatggg cgttggcgcg accctggttt gctctctgat
     3661 ggtgtctggt cgtctgtggg gtctgatcgc ttgccaccac tacgaaccgc gcttcgttcc
     3721 gttccacatt cgcgctgctg gcgaagcgct ggcggaaact tgtgcgatcc gcatcgcgac
     3781 gctggagagc tttgcacagt ctcagtccaa aggtggatcc ggtcctgctg ccaagagggt
     3841 caagttggac taggcggccg caactcccac ctgcaacatg cgtgactgac tgaggccgcg
     3901 actctagagt cgacctgcag gcatgcaagc ttgatatcaa gcttatcgat aatcaacctc
     3961 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     4021 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     4081 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     4141 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     4201 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     4261 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     4321 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
     4381 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     4441 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     4501 ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccgt cgacctcgag
     4561 ggaattaatt cgagctcggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
     4621 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa
     4681 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     4741 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     4801 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     4861 tcagtgtgga aaatctctag cagcatctag aattaattcc gtgtattcta tagtgtcacc
     4921 taaatcgtat gtgtatgata cataaggtta tgtattaatt gtagccgcgt tctaacgaca
     4981 atatgtacaa gcctaattgt gtagcatctg gcttactgaa gcagacccta tcatctctct
     5041 cgtaaactgc cgtcagagtc ggtttggttg gacgaacctt ctgagtttct ggtaacgccg
     5101 tcccgcaccc ggaaatggtc agcgaaccaa tcagcagggt catcgctagc cagatcctct
     5161 acgccggacg catcgtggcc ggcatcaccg gcgccacagg tgcggttgct ggcgcctata
     5221 tcgccgacat caccgatggg gaagatcggg ctcgccactt cgggctcatg agcgcttgtt
     5281 tcggcgtggg tatggtggca ggccccgtgg ccgggggact gttgggcgcc atctccttgc
     5341 atgcaccatt ccttgcggcg gcggtgctca acggcctcaa cctactactg ggctgcttcc
     5401 taatgcagga gtcgcataag ggagagcgtc gaatggtgca ctctcagtac aatctgctct
     5461 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     5521 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     5581 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc
     5641 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     5701 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     5761 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     5821 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     5881 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     5941 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     6001 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     6061 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     6121 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     6181 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     6241 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     6301 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     6361 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     6421 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     6481 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     6541 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     6601 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     6661 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     6721 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     6781 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     6841 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     6901 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     6961 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     7021 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     7081 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     7141 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     7201 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     7261 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     7321 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     7381 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     7441 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     7501 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     7561 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     7621 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctgtgga
     7681 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     7741 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     7801 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     7861 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     7921 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     7981 gaggcttttt tggaggccta ggcttttgca aaaagcttgg acacaagaca ggcttgcgag
     8041 atatgtttga gaataccact ttatcccgcg tcagggagag gcagtgcgta aaaagacgcg
     8101 gactcatgtg aaatactggt ttttagtgcg ccagatctct ataatctcgc gcaacctatt
     8161 ttcccctcga acacttttta agccgtagat aaacaggctg ggacacttca catgagcgaa
     8221 aaatacatcg tcacctggga catgttgcag atccatgcac gtaaactcgc aatccgactg
     8281 atgccttctg aacaatggaa aggcattatt gccgtaagcc gtggcggtct gtaccgggtg
     8341 cgttactggc gcgtgaactg ggtattcgtc atgtcgatac cgtttgtatt tccagctacg
     8401 atcacgacaa ccagcgcgag cttaaagtgc tgaaacgcgc agaaggcgat ggcgaaggct
     8461 tcatcgttat tgatgacctg gtggataccg gtggtactgc ggttgcgatt cgtgaaatgt
     8521 atccaaaagc gcactttgtc accatcttcg caaaaccggc tggtcgtccg ctggttgatg
     8581 actatgttgt tgatatcccg caagatacct ggattgaaca gccgtgggat atgggcgtcg
     8641 tattcgtccc gccaatctcc ggtcgctaat cttttcaacg cctggcactg ccgggcgttg
     8701 ttctttttaa cttcaggcgg gttacaatag tttccagtaa gtattctgga ggctgcatcc
     8761 atgacacagg caaacctgag cgaaaccctg ttcaaacccc gctttaaaca tcctgaaacc
     8821 tcgacgctag tccgccgctt taatcacggc gcacaaccgc ctgtgcagtc ggcccttgat
     8881 ggtaaaacca tccctcactg gtatcgcatg attaaccgtc tgatgtggat ctggcgcggc
     8941 attgacccac gcgaaatcct cgacgtccag gcacgtattg tgatgagcga tgccgaacgt
     9001 accgacgatg atttatacga tacggtgatt ggctaccgtg gcggcaactg gatttatgag
     9061 tgggccccgg atctttgtga aggaacctta cttctgtggt gtgacataat tggacaaact
     9121 acctacagag atttaaagct ctaaggtaaa tataaaattt ttaagtgtat aatgtgttaa
     9181 actactgatt ctaattgttt gtgtatttta gattccaacc tatggaactg atgaatggga
     9241 gcagtggtgg aatgccttta atgaggaaaa cctgttttgc tcagaagaaa tgccatctag
     9301 tgatgatgag gctactgctg actctcaaca ttctactcct ccaaaaaaga agagaaaggt
     9361 agaagacccc aaggactttc cttcagaatt gctaagtttt ttgagtcatg ctgtgtttag
     9421 taatagaact cttgcttgct ttgctattta caccacaaag gaaaaagctg cactgctata
     9481 caagaaaatt atggaaaaat attctgtaac ctttataagt aggcataaca gttataatca
     9541 taacatactg ttttttctta ctccacacag gcatagagtg tctgctatta ataactatgc
     9601 tcaaaaattg tgtaccttta gctttttaat ttgtaaaggg gttaataagg aatatttgat
     9661 gtatagtgcc ttgactagag atcataatca gccataccac atttgtagag gttttacttg
     9721 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
     9781 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     9841 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     9901 tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat cccaaacttc
     9961 ccaccccata ccctattacc actgccaatt acctgtggtt tcatttactc taaacctgtg
    10021 attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact
    10081 tagtagttgg aagggctaat tcactcccaa agaagacaag atatccttga tctgtggatc
    10141 taccacacac aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga
    10201 tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa
    10261 gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat
    10321 gacccggaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg
    10381 gcccgagagc tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga
    10441 ctttccgctg gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc
    10501 ctcagatcct gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca
    10561 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
    10621 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
    10681 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac
    10741 ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg
    10801 cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga
    10861 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg
    10921 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata
    10981 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca
    11041 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa
    11101 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata
    11161 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc
    11221 gcacagcaag cggccggccg ctgatcttca gacctggagg aggagatatg agggacaatt
    11281 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
    11341 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
    11401 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg
    11461 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
    11521 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
    11581 gaatc
//
