LOCUS       sequence-442292-       10544 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A lentiviral vector expressing BFP and the TetON transcription 
            factor rtTA..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10544)
  AUTHORS   Brumbaugh-Reed EH, Aoki K, Toettcher JE
  TITLE     Rapid and reversible dissolution of biomolecular condensates using 
            light-controlled recruitment of a solubility tag
  JOURNAL   bioRxiv 2024.01.16.575860
REFERENCE   2  (bases 1 to 10544)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.01.16.575860"
COMMENT     Sequence Label: pHR hEF1A-BFP-P2A-rtTA-WPRE
FEATURES             Location/Qualifiers
     source          1..10544
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             253..297
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    582..699
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        852..1259
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             1331..2029
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     CDS             2039..2095
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2096..2842
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    2940..3528
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2993..3013)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3411..3422)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3531..3547)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3532..3548)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3638..3871
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(3896..3914)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3897..3914)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4204..4223)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(4437..4456)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4556..4578
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4616..4634)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4702..4806
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4807..4875,4876..5667)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5025..5044)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5838..6426
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6327..6346
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6580..6597
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(6667..6687)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6672..7001
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6852..6987
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          8135..8200
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8330..8350
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8775..8909
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8812..8831)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8866..8885
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9077..9710
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9757..9882
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    join(10379..10544,1..68)
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa
       61 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg
      121 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag
      181 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa
      241 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg
      301 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga
      361 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag
      421 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc
      481 gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac
      541 ggatctcgac ggtatcgcca aatggcagta ttcatccaca attttaaaag aaaagggggg
      601 attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact
      661 aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc
      721 agagatccag tttggatcga taagcttgat atcgaattcc tgcagccccg ataaaataaa
      781 agattttatt tagtctccag aaaaaggggg gaatgaaaga ccccacctgt aggtttggca
      841 agctagctgc agtaacgcca ttttgcaagg catggaaaaa taccaaacca agaatagaga
      901 agttcagatc aagggcgggt acatgaaaat agctaacgtt gggccaaaca ggatatctgc
      961 ggtgagcagt ttcggccccg gcccggggcc aagaacagat ggtcaccgca gtttcggccc
     1021 cggcccgagg ccaagaacag atggtcccca gatatggccc aaccctcagc agtttcttaa
     1081 gacccatcag atgtttccag gctcccccaa ggacctgaaa tgaccctgcg ccttatttga
     1141 attaaccaat cagcctgctt ctcgcttctg ttcgcgcgct tctgcttccc gagctctata
     1201 aaagagctca caacccctca ctcggcgcgc cagtcctccg acagactgag tcgcccgggg
     1261 gggatctgga gctctcgaga attctcacgc gtcaagtgga gcaaggcagg tggacagtgg
     1321 atccgccacc atgagcgagc tgattaagga gaacatgcac atgaagctgt acatggaggg
     1381 caccgtggac aaccatcact tcaagtgcac atccgagggc gaaggcaagc cctacgaggg
     1441 cacccagacc atgagaatca aggtggtcga gggcggccct ctccccttcg ccttcgacat
     1501 cctggctact agcttcctct acggcagcaa gaccttcatc aaccacaccc agggcatccc
     1561 cgacttcttc aagcagtcct tccctgaggg cttcacatgg gagagagtca ccacatacga
     1621 agacgggggc gtgctgaccg ctacccagga caccagcctc caggacggct gcctcatcta
     1681 caacgtcaag atcagagggg tgaacttcac atccaacggc cctgtgatgc agaagaaaac
     1741 actcggctgg gaggccttca ccgagacgct gtaccccgct gacggcggcc tggaaggcag
     1801 aaacgacatg gccctgaagc tcgtgggcgg gagccatctg atcgcaaaca tcaagaccac
     1861 atatagatcc aagaaacccg ctaagaacct caagatgcct ggcgtctact atgtggacta
     1921 cagactggaa agaatcaagg aggccaacaa cgagacctac gtcgagcagc acgaggtggc
     1981 agtggccaga tactgcgacc tccctagcaa actggggcac aagcttaatg gaagcggagc
     2041 tactaacttc agcctgctga agcaggctgg agacgtggag gagaaccctg gacctatgag
     2101 ccggctggac aagagcaaag tgatcaacag cgccctggaa ctgctgaacg gcgtgggcat
     2161 cgagggcctg accacccgga agctggccca gaaactgggc gtggaacagc ccaccctgta
     2221 ctggcacgtg aagaacaagc gggccctgct ggacgccctg cccatcgaga tgctggaccg
     2281 gcaccacacc cacagctgcc ctctggaagg cgagagctgg caggacttcc tgcggaacaa
     2341 cgccaagagc tacagatgcg ccctgctgag ccaccgggac ggcgccaaag tgcacctggg
     2401 caccagaccc accgagaagc agtacgagac actggaaaac cagctggcct tcctgtgcca
     2461 gcagggcttc agcctggaaa acgccctgta cgccctgagc gccgtgggcc acttcaccct
     2521 gggctgcgtg ctggaagaac aggaacacca ggtcgccaaa gaggaaagag agacacccac
     2581 caccgacagc atgccccccc tgctgaagca ggccatcgag ctgttcgaca gacagggcgc
     2641 cgagcccgcc ttcctgttcg gcctggaact gatcatctgc ggcctcgaga agcagctgaa
     2701 gtgcgagagc ggcggaccca ccgacgccct ggacgacttc gacctggaca tgctgcccgc
     2761 cgatgccctg gatgattttg atctggatat gctccctgct gacgctctcg atgacttcga
     2821 tctcgatatg ctgccaggct aggcggccgc aactcccacc tgcaacatgc gtgactgact
     2881 gaggccgcga ctctagagtc gacctgcagg catgcaagct tgatatcaag cttatcgata
     2941 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     3001 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     3061 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     3121 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     3181 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     3241 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     3301 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg
     3361 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     3421 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     3481 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgtc
     3541 gacctcgagg gaattaattc gagctcggta cctttaagac caatgactta caaggcagct
     3601 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
     3661 cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc
     3721 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     3781 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     3841 cccttttagt cagtgtggaa aatctctagc agcatctaga attaattccg tgtattctat
     3901 agtgtcacct aaatcgtatg tgtatgatac ataaggttat gtattaattg tagccgcgtt
     3961 ctaacgacaa tatgtacaag cctaattgtg tagcatctgg cttactgaag cagaccctat
     4021 catctctctc gtaaactgcc gtcagagtcg gtttggttgg acgaaccttc tgagtttctg
     4081 gtaacgccgt cccgcacccg gaaatggtca gcgaaccaat cagcagggtc atcgctagcc
     4141 agatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg
     4201 gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga
     4261 gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca
     4321 tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg
     4381 gctgcttcct aatgcaggag tcgcataagg gagagcgtcg aatggtgcac tctcagtaca
     4441 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
     4501 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     4561 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc
     4621 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     4681 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     4741 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     4801 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     4861 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     4921 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     4981 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     5041 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     5101 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     5161 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     5221 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     5281 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     5341 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     5401 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     5461 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     5521 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     5581 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     5641 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     5701 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     5761 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     5821 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     5881 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     5941 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     6001 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     6061 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     6121 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     6181 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     6241 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     6301 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     6361 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     6421 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     6481 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     6541 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     6601 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     6661 agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     6721 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     6781 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     6841 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     6901 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     6961 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagcttgga cacaagacag
     7021 gcttgcgaga tatgtttgag aataccactt tatcccgcgt cagggagagg cagtgcgtaa
     7081 aaagacgcgg actcatgtga aatactggtt tttagtgcgc cagatctcta taatctcgcg
     7141 caacctattt tcccctcgaa cactttttaa gccgtagata aacaggctgg gacacttcac
     7201 atgagcgaaa aatacatcgt cacctgggac atgttgcaga tccatgcacg taaactcgca
     7261 atccgactga tgccttctga acaatggaaa ggcattattg ccgtaagccg tggcggtctg
     7321 taccgggtgc gttactggcg cgtgaactgg gtattcgtca tgtcgatacc gtttgtattt
     7381 ccagctacga tcacgacaac cagcgcgagc ttaaagtgct gaaacgcgca gaaggcgatg
     7441 gcgaaggctt catcgttatt gatgacctgg tggataccgg tggtactgcg gttgcgattc
     7501 gtgaaatgta tccaaaagcg cactttgtca ccatcttcgc aaaaccggct ggtcgtccgc
     7561 tggttgatga ctatgttgtt gatatcccgc aagatacctg gattgaacag ccgtgggata
     7621 tgggcgtcgt attcgtcccg ccaatctccg gtcgctaatc ttttcaacgc ctggcactgc
     7681 cgggcgttgt tctttttaac ttcaggcggg ttacaatagt ttccagtaag tattctggag
     7741 gctgcatcca tgacacaggc aaacctgagc gaaaccctgt tcaaaccccg ctttaaacat
     7801 cctgaaacct cgacgctagt ccgccgcttt aatcacggcg cacaaccgcc tgtgcagtcg
     7861 gcccttgatg gtaaaaccat ccctcactgg tatcgcatga ttaaccgtct gatgtggatc
     7921 tggcgcggca ttgacccacg cgaaatcctc gacgtccagg cacgtattgt gatgagcgat
     7981 gccgaacgta ccgacgatga tttatacgat acggtgattg gctaccgtgg cggcaactgg
     8041 atttatgagt gggccccgga tctttgtgaa ggaaccttac ttctgtggtg tgacataatt
     8101 ggacaaacta cctacagaga tttaaagctc taaggtaaat ataaaatttt taagtgtata
     8161 atgtgttaaa ctactgattc taattgtttg tgtattttag attccaacct atggaactga
     8221 tgaatgggag cagtggtgga atgcctttaa tgaggaaaac ctgttttgct cagaagaaat
     8281 gccatctagt gatgatgagg ctactgctga ctctcaacat tctactcctc caaaaaagaa
     8341 gagaaaggta gaagacccca aggactttcc ttcagaattg ctaagttttt tgagtcatgc
     8401 tgtgtttagt aatagaactc ttgcttgctt tgctatttac accacaaagg aaaaagctgc
     8461 actgctatac aagaaaatta tggaaaaata ttctgtaacc tttataagta ggcataacag
     8521 ttataatcat aacatactgt tttttcttac tccacacagg catagagtgt ctgctattaa
     8581 taactatgct caaaaattgt gtacctttag ctttttaatt tgtaaagggg ttaataagga
     8641 atatttgatg tatagtgcct tgactagaga tcataatcag ccataccaca tttgtagagg
     8701 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
     8761 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     8821 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     8881 tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc
     8941 ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt catttactct
     9001 aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta
     9061 ctacaaactt agtagttgga agggctaatt cactcccaaa gaagacaaga tatccttgat
     9121 ctgtggatct accacacaca aggctacttc cctgattagc agaactacac accagggcca
     9181 ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt tgagccagat
     9241 aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt gagcctgcat
     9301 gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg cctagcattt
     9361 catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata tcgagcttgc
     9421 tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg gactggggag
     9481 tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg ggtctctctg
     9541 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     9601 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     9661 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
     9721 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
     9781 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
     9841 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
     9901 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
     9961 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
    10021 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
    10081 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
    10141 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
    10201 aagaccaccg cacagcaagc ggccggccgc tgatcttcag acctggagga ggagatatga
    10261 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
    10321 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
    10381 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
    10441 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
    10501 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtc
//
