LOCUS       sequence-442312-       10965 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A complete OptoMBP cassette for dissolution of FUS-CHOP condensates.
            .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10965)
  AUTHORS   Brumbaugh-Reed EH, Aoki K, Toettcher JE
  TITLE     Rapid and reversible dissolution of biomolecular condensates using 
            light-controlled recruitment of a solubility tag
  JOURNAL   bioRxiv 2024.01.16.575860
REFERENCE   2  (bases 1 to 10965)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.01.16.575860"
COMMENT     Sequence Label: 
            pPB-TetOn-FUS-CHOP-FusionRed-FRB-iLID-WPRE-SFFV-MBP-SspB-irFP-NLS-WP
            RE
FEATURES             Location/Qualifiers
     source          1..10965
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(24..43)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     143..165
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(203..221)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        289..393
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(394..462,463..1254)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(612..631)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1425..2013
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1914..1933
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2167..2184
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2301..2322
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2337..2342,2343..2360,2361..2367)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2375..2391
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2380..2402
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2399..2415
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2399..2415
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   2450..2484
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        2865..3243
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     protein_bind    2873..2891
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2909..2927
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2945..2963
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2981..2999
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3017..3035
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3053..3071
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3089..3107
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     3152..3176
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    3289..3313
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3322..3351
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3732..3743
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             4498..5193
                     /codon_start=1
                     /product="monomeric red fluorescent protein optimized for 
                     use as a fusion tag (Shemiakina et al., 2012)"
                     /label=FusionRed
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMPMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFMYGSRTFIKHPPGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKVRGVNFPANGPVMQKKTLGWEASTETMYPADGGLEGACDMAL
                     KLVGGGHLICNLETTYRSKKPATNLKMPGVYNVDHRLERIKEADDETYVEQHEVAVARY
                     STGGAGDGGK"
     CDS             5206..5484
                     /codon_start=1
                     /product="T2098L mutant of FKBP-rapamycin binding domain of
                     human FRAP"
                     /label=FRB*
                     /note="binds synthetic ligands such as AP21967"
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRISK"
     CDS             5527..5964
                     /codon_start=1
                     /product="improved light-inducible dimer when used with 
                     SspB nano or SspB micro (Guntas et al., 2015)"
                     /label=iLID
                     /translation="LATTLERIEKNFVITDPRLPDNPIIFASDSFLQLTEYSREEILGR
                     NCRFLQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFWNVFHLQPMRDYKGDVQ
                     YFIGVQLDGTERLHGAAEREAVCLIKKTAFQIAEAANDENYF"
     misc_feature    6072..6660
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(6543..6554)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        6813..7220
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      7286..7295
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7295..7324
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             7337..8434
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="KIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEE
                     KFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLI
                     AYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAA
                     DGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGET
                     AMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLE
                     NYLLTDEGLEAVNKDKPLGAVALKSYEEELVKDPRIAATMENAQKGEIMPNIPQMSAFW
                     YAVRTAVINAASGRQTVDEALKDAQT"
     primer_bind     8408..8431
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             8510..8839
                     /codon_start=1
                     /product="SspB from Haemophilus influenzae, with the 
                     mutations Y11K and A15E to disrupt the natural dimerization
                     of the protein"
                     /label=SspB nano
                     /note="dimerizes with iLID at high affinity to create iLID 
                     nano (Guntas et al., 2015)"
                     /translation="SSPKRPKLLREYYDWLVDNSFTPYLVVDATYLGVNVPVEYVKDGQ
                     IVLNLSASATGNLQLTNDFIQFNARFKGVSRELYIPMGAALAIYARENGDGVMFEPEEI
                     YDELNI"
     CDS             8843..9802
                     /codon_start=1
                     /product="naturally monomeric infrared fluorescent protein 
                     derived from a truncated bacteriophytochrome (Yu et al., 
                     2015)"
                     /label=mIFP
                     /translation="MSVPLTTSAFGHAFLANCEREQIHLAGSIQPHGILLAVKEPDNVV
                     IQASINAAEFLNTNSVVGRPLRDLGGDLPLQILPHLNGPLHLAPMTLRCTVGSPPRRVD
                     CTIHRPSNGGLIVELEPATKTTNIAPALDGAFHRITSSSSLMGLCDETATIIREITGYD
                     RVMVVRFDEEGNGEILSERRRADLEAFLGNRYPASTIPQIARRLYEHNRVRLLVDVNYT
                     PVPLQPRISPLNGRDLDMSLSCLRSMSPIHQKYMQDMGVGATLVCSLMVSGRLWGLIAC
                     HHYEPRFVPFHIRAAGEALAETCAIRIATLESFAQSQSK"
     CDS             9815..9841
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     misc_feature    9942..10530
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(10413..10424)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(10533..10549)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10534..10550)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     repeat_region   10815..10877
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc
       61 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
      121 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
      181 ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
      241 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
      301 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
      361 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
      421 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
      481 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
      541 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
      601 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
      661 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
      721 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
      781 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
      841 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
      901 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
      961 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     1021 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     1081 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     1141 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     1201 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     1261 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     1321 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     1381 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     1441 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     1501 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     1561 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     1621 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     1681 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     1741 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     1801 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     1861 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     1921 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     1981 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     2041 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     2101 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     2161 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     2221 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     2281 ggcagtgatc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     2341 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     2401 ggaaacagct atgaccatga ttacgccggg gcgcgccatt ctagattaac cctagaaaga
     2461 tagtctgcgt aaaattgacg catgcattct tgaaatattg ctctctcttt ctaaatagcg
     2521 cgaatccgtc gctgtgcatt taggacatct cagtcgccgc ttggagctcc cgtgaggcgt
     2581 gcttgtcaat gcggtaagtg tcactgattt tgaactataa cgaccgcgtg agtcaaaatg
     2641 acgcatgatt atcttttacg tgacttttaa gatttaactc atacgataat tatattgtta
     2701 tttcatgttc tacttacgtg ataacttatt atatatatat tttcttgtta tagatatcaa
     2761 ctagaatgct agcatgggcc cagatctccg aggggacccg acaggcccgc agggacagca
     2821 gagatccagt ttggatcgat cgatcacgag actagcctcg agttggcttt actccctatc
     2881 agtgatagag aacgtatgaa gagtttactc cctatcagtg atagagaacg tatgcagact
     2941 ttactcccta tcagtgatag agaacgtata aggagtttac tccctatcag tgatagagaa
     3001 cgtatgacca gtttactccc tatcagtgat agagaacgta tctacagttt actccctatc
     3061 agtgatagag aacgtatatc cagtttactc cctatcagtg atagagaacg tataagcttt
     3121 aggcgtgtac ggtgggcgcc tataaaagca gagctcgttt agtgaaccgt cagatcgcct
     3181 ggagcaattc cacaacactt ttgtcttata ccaactttcc gtaccacttc ctaccctcgt
     3241 aaaccggttc gtcgactagt ccagtgtggt ggaattctgc agatatcaac aagtttgtac
     3301 aaaaaagcag gctccaccat ggaacaaaaa ctcatctcag aagaggatct gatggcctca
     3361 aacgattata cccaacaagc aacccaaagc tatggggcct accccaccca gcccgggcag
     3421 ggctattccc agcagagcag tcagccctac ggacagcaga gttacagtgg ttatagccag
     3481 tccacggaca cttcaggcta tggccagagc agctattctt cttatggcca gagccagaac
     3541 acaggctatg gaactcagtc aactccccag ggatatggct cgactggcgg ctatggcagt
     3601 agccagagct cccaatcgtc ttacgggcag cagtcctcct atcctggcta tggccagcag
     3661 ccagctccca gcagcacctc gggaagttac ggtagcagtt ctcagagcag cagctatggg
     3721 cagccccaga gtgggagcta cagccagcag cctagctatg gtggacagca gcaaagctat
     3781 ggacagcagc aaagctataa tccccctcag ggctatggac agcagaacca gtacaacagc
     3841 agcagtggtg gtggaggtgg aggtggaggt ggaggtaact atggccaaga tcaatcctcc
     3901 atgagtagtg gtggtggcag tggtggcggt tatggcaatc aagaccagag tggtggaggt
     3961 ggcagcggtg gctatggaca gcaggaccgt ggagcagctg agtcattgcc tttctccttc
     4021 gggacactgt ccagctggga gctggaagcc tggtatgagg acctgcaaga ggtcctgtct
     4081 tcagatgaaa atgggggtac ctatgtttca cctcctggaa atgaagagga agaatcaaaa
     4141 atcttcacca ctcttgaccc tgcttctctg gcttggctga ctgaggagga gccagaacca
     4201 gcagaggtca caagcacctc ccagagccct cactctccag attccagtca gagctccctg
     4261 gctcaggagg aagaggagga agaccaaggg agaaccagga aacggaaaca gagtggtcat
     4321 tccccagccc gggctggaaa gcagcgcatg aaggagaaag aacaggagaa tgaaaggaaa
     4381 gtggcacagc tagctgaaga gaatgaacgg ctcaagcagg aaatcgagcg cctgaccagg
     4441 gaagtagagg cgactcgccg agctctgatt gaccgaatgg tgaatctgca ccaagcaatg
     4501 gtgagcgagc tgattaagga gaacatgccc atgaagctgt acatggaggg caccgtgaac
     4561 aaccaccact tcaagtgcac atccgagggc gaaggcaagc cctacgaggg cacccagacc
     4621 atgagaatca aggtcgtcga gggcggccct ctccccttcg ccttcgacat cctggctacc
     4681 agcttcatgt acggcagcag aaccttcatc aagcaccctc cgggcatccc cgacttcttt
     4741 aagcagtcct tccctgaggg cttcacatgg gagagagtca ccacatacga agacgggggc
     4801 gtgctgaccg ctacccagga caccagcctc caggacggct gcctcatcta caacgtcaag
     4861 gttagagggg tgaacttccc agccaacggc cctgtgatgc agaagaaaac actcggctgg
     4921 gaggcctcca ccgagacgat gtaccccgct gacggcggcc tggaaggcgc atgtgacatg
     4981 gccctgaagc tcgtgggcgg gggccacctg atctgcaacc ttgagaccac atacagatcc
     5041 aagaaacccg ctacgaacct caagatgccc ggcgtctaca acgtggacca cagactggaa
     5101 agaatcaagg aggccgacga tgagacctac gtcgagcagc acgaggtggc tgtggccaga
     5161 tactctactg gtggcgctgg tgatggaggt aaagcggcca cgcgtatcct ctggcatgag
     5221 atgtggcatg aaggcctgga agaggcatct cgtttgtact ttggggaaag gaacgtgaaa
     5281 ggcatgtttg aggtgctgga gcccttgcat gctatgatgg aacggggccc ccagactctg
     5341 aaggaaacat cctttaatca ggcctatggt cgagatttaa tggaggccca agagtggtgc
     5401 aggaagtaca tgaaatcagg gaatgtcaag gacctcctcc aagcctggga cctctattat
     5461 catgtgttcc gacgaatctc aaagggaagc ggaggtagtg ctggtggtgg tggatccggt
     5521 gagtttctgg caaccacact ggaacggatc gagaaaaatt tcgtgattac tgatccgaga
     5581 ctgcctgaca acccaatcat ttttgcgagc gattccttcc tgcagctgac agaatattct
     5641 cgggaagaga tcctggggcg caattgccgt tttctgcagg gacccgagac agaccgtgcc
     5701 actgttcgga aaatcagaga tgctattgac aaccagactg aagtgaccgt tcagctgatc
     5761 aattatacca agagcggcaa gaagttctgg aacgtgttcc acctgcagcc gatgcgcgat
     5821 tataagggcg acgtccagta cttcattggc gtgcagctgg atggcaccga acgtcttcat
     5881 ggcgccgctg agcgtgaggc ggtctgcctg atcaaaaaga cagcctttca gattgctgag
     5941 gcagcgaacg acgaaaatta cttttgacct gcaggcggcc gcaactccca cctgcaacat
     6001 gcgtgactga ctgaggccgc gactctagag tcgacctgca ggcatgcaag cttgatatca
     6061 agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     6121 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     6181 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     6241 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     6301 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     6361 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     6421 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     6481 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     6541 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     6601 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     6661 atcgcccgcc ccacgacccg cagcgcccga ccgaaaggag cgcacgaccc catgcatcga
     6721 tactagttcc gataaaataa aagattttat ttagtctcca gaaaaagggg ggaatgaaag
     6781 accccacctg taggtttggc aagctagctg cagtaacgcc attttgcaag gcatggaaaa
     6841 ataccaaacc aagaatagag aagttcagat caagggcggg tacatgaaaa tagctaacgt
     6901 tgggccaaac aggatatctg cggtgagcag tttcggcccc ggcccggggc caagaacaga
     6961 tggtcaccgc agtttcggcc ccggcccgag gccaagaaca gatggtcccc agatatggcc
     7021 caaccctcag cagtttctta agacccatca gatgtttcca ggctccccca aggacctgaa
     7081 atgaccctgc gccttatttg aattaaccaa tcagcctgct tctcgcttct gttcgcgcgc
     7141 ttctgcttcc cgagctctat aaaagagctc acaacccctc actcggcgcg ccagtcctcc
     7201 gacagactga gtcgcccggg ggggatctgg agctctcgag aattctcacg cgtcaagtgg
     7261 agcaaggcag gtggacagtg gatccgccac catggaacaa aaactcatct cagaagagga
     7321 tctgggagga gggtctaaaa tcgaagaagg taaactggta atctggatta acggcgataa
     7381 aggctataac ggtctcgctg aagtcggtaa gaaattcgag aaagataccg gaattaaagt
     7441 caccgttgag catccggata aactggaaga gaaattccca caggttgcgg caactggcga
     7501 tggccctgac attatcttct gggcacacga ccgctttggt ggctacgctc aatctggcct
     7561 gttggctgaa atcaccccgg acaaagcgtt ccaggacaag ctgtatccgt ttacctggga
     7621 tgccgtacgt tacaacggca agctgattgc ttacccgatc gctgttgaag cgttatcgct
     7681 gatttataac aaagatctgc tgccgaaccc gccaaaaacc tgggaagaga tcccggcgct
     7741 ggataaagaa ctgaaagcga aaggtaagag cgcgctgatg ttcaacctgc aagaaccgta
     7801 cttcacctgg ccgctgattg ctgctgacgg gggttatgcg ttcaagtatg aaaacggcaa
     7861 gtacgacatt aaagacgtgg gcgtggataa cgctggcgcg aaagcgggtc tgaccttcct
     7921 ggttgacctg attaaaaaca aacacatgaa tgcagacacc gattactcca tcgcagaagc
     7981 tgcctttaat aaaggcgaaa cagcgatgac catcaacggc ccgtgggcat ggtccaacat
     8041 cgacaccagc aaagtgaatt atggtgtaac ggtactgccg accttcaagg gtcaaccatc
     8101 caaaccgttc gttggcgtgc tgagcgcagg tattaacgcc gccagtccga acaaagagct
     8161 ggcaaaagag ttcctcgaaa actatctgct gactgatgaa ggtctggaag cggttaataa
     8221 agacaaaccg ctgggtgccg tagcgctgaa gtcttacgag gaagagttgg tgaaagatcc
     8281 gcgtattgcc gccactatgg aaaacgccca gaaaggtgaa atcatgccga acatcccgca
     8341 gatgtccgct ttctggtatg ccgtgcgtac tgcggtgatc aacgccgcca gcggtcgtca
     8401 gactgtcgat gaagccctga aagacgcgca gacttccgga ctcagatctc gagctcaagc
     8461 ttcgaacgaa ttcggcattg atctgagcgg cctgaccctg caggaattca gctccccgaa
     8521 acgccctaag ctgctgcgtg aatattacga ttggctggtt gataacagct ttaccccata
     8581 tctggtggtg gatgccacat acctgggcgt gaacgtgccc gtggagtatg tgaaagacgg
     8641 tcagatcgtg ctgaatctgt ctgcaagtgc gaccggcaac ctgcaactga caaatgattt
     8701 tatccagttc aacgcccgct ttaagggcgt gtctcgtgaa ctgtatatcc cgatgggtgc
     8761 cgctctggcc atttacgctc gcgagaacgg cgatggtgtg atgttcgaac cagaagaaat
     8821 ctatgacgag ctgaatattg gtatgtcggt accgctgact acctcagcat tcggccacgc
     8881 gtttctggct aactgtgaac gcgagcagat ccacctggcg ggctccattc agccgcacgg
     8941 tatcctgctg gctgtgaaag agccggacaa cgtggtgatc caggcttcta ttaacgctgc
     9001 ggagttcctg aacaccaact ctgttgttgg ccgtccgctg cgtgacctgg gcggcgatct
     9061 gcctttgcag atcctgccgc acctgaacgg cccgctgcac ctggctccga tgaccctgcg
     9121 ttgtaccgtg ggttctccgc cgcgtcgtgt ggactgtacc attcatcgtc cgtctaacgg
     9181 cggcctgatc gtagaactgg aaccagcaac caagaccact aacattgcgc cggctctgga
     9241 cggtgcgttt catcgtatca cttcttcatc ctccctgatg ggcctgtgtg acgaaaccgc
     9301 gactattatc cgtgagatta ctggctacga ccgtgtgatg gtagtacgtt tcgatgaaga
     9361 gggtaatggc gaaattctgt ccgaacgtcg tcgtgcggac ctggaagcgt tcctgggtaa
     9421 ccgctacccg gcgtctacta ttccgcagat cgctcgtcgc ctgtacgaac ataaccgtgt
     9481 tcgcctgctg gtagatgtga actatactcc ggttccgcta cagccgcgca tcagcccgct
     9541 gaacggtcgt gatctggata tgtccctgtc ttgcctgcgc tctatgtccc cgatccacca
     9601 gaaatacatg caggacatgg gcgttggcgc gaccctggtt tgctctctga tggtgtctgg
     9661 tcgtctgtgg ggtctgatcg cttgccacca ctacgaaccg cgcttcgttc cgttccacat
     9721 tcgcgctgct ggcgaagcgc tggcggaaac ttgtgcgatc cgcatcgcga cgctggagag
     9781 ctttgcacag tctcagtcca aaggtggatc cggtcctgct gccaagaggg tcaagttgga
     9841 ctaggcggcc gcaactccca cctgcaacat gcgtgactga ctgaggccgc gactctagag
     9901 tcgacctgca ggcatgcaag cttgatatca agcttatcga taatcaacct ctggattaca
     9961 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
    10021 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
    10081 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
    10141 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
    10201 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
    10261 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
    10321 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga
    10381 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
    10441 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
    10501 gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgacctcga gggaattaat
    10561 tcgagctcgg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
    10621 ttaaaagaaa aggggggact aaaagttttg ttactttata gaagaaattt tgagtttttg
    10681 ttttttttta ataaataaat aaacataaat aaattgtttg ttgaatttat tattagtatg
    10741 taagtgtaaa tataataaaa cttaatatct attcaaatta ataaataaac ctcgatatac
    10801 agaccgataa aacacatgcg tcaattttac gcatgattat ctttaacgta cgtcacaata
    10861 tgattatctt tctagggtta atctagtata cgcgtatgcg gccgcttaat taatccatgg
    10921 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttca
//
