LOCUS       sequence-442319-       16513 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16513)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 16513)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0662
FEATURES             Location/Qualifiers
     source          1..16513
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    12370..12958
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(12423..12443)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(12841..12852)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             13030..13263
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    13341..13462
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13378..13397)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     13432..13451
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      13502..13637
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(13657..13676)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(13658..13676)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(13686..13703)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(13686..13702)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13695..13717)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      13844..14299
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(13931..13950)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     14141..14162
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        14325..14429
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(14430..14498,14499..15290)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(14648..14667)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      15461..16049
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     15950..15969
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     16203..16220
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    16337..16358
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(16373..16378,16379..16396,16397..16403)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    16411..16427
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     16416..16438
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     16435..16451
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     16435..16451
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     16470..16490
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        16472..16490
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgaaa atgaaaatac agaaaaaaga
     4141 gaagcagttg tcaaatttaa aagttttgaa tcactcccca atgtctgatg cctctgtcaa
     4201 ttttgactac aaatctccat ccccatttga ctgcagcact gatcaagaag agaaaattga
     4261 agatgttgct agtcactgtc tgcctcagaa ggacctgtat actgctgaag aggaagctgc
     4321 tacccttttt cctaggaaaa tgacatccca taatgggatg gaggacagtg gaggaggagg
     4381 tactggagtg aagaagaaac ggaagaaaaa ggagccagga gaccaagagg gtgcagcaaa
     4441 gggaagcaag gacagagagc ccaagccaaa gaggaaacga gaaccgaaag agccaaagga
     4501 acccagaaag gccaaggagc cgaagaaggc caaggagcac aaggagccga agcaaaaaga
     4561 tggggcaaag aaggcacgga agccccggga ggcctcgggc accaaggagg ccaaagagaa
     4621 gaggagctgc actgactctg cagccaggac gaagtccagg aaggccagca aggagcaagg
     4681 accaacccca gtggagaaaa agaagaaagg aaaaaggaaa agtgaaacta cagtggagag
     4741 tttagagctg gatcagggcc tgacgaaccc atctctgcgg agtcctgagg agtccactga
     4801 gtctacagac agccagaaac gacgctcggg aaggcaagta aagcgcagaa aatacaatga
     4861 ggacctggac ttcaaagtgg tggatgatga tggggaaaca attgctgttc ttggagctgg
     4921 tcgaacatct gcactctcag cctctacact ggcctggcag gcggaggagc ctccagaaga
     4981 tgatgcaaac atcattgaga agatcctggc atctaagact gtccaggagg ttcacccagg
     5041 agaacctccg ttcgacttgg agctgttcta cgttaagtat agaaattttt cctacttaca
     5101 ttgtaaatgg gccacaatgg aagagctcga aaaggatcct cgcatcgcac agaagatcaa
     5161 gcgatttagg aataaacaag cccagatgaa gcacattttt acggagcctg atgaagactt
     5221 gttcaatcca gactatgtag aagttgatcg catcttggag gtggcccaca ccaaggatgc
     5281 agaaacaggg gaggaggtaa cacattacct ggtgaagtgg tgctcactac catatgaaga
     5341 aagcacgtgg gagctagagg aagatgtaga tcctgcaaaa gttaaagaat ttgaatctct
     5401 tcaagttctc cctgaaatta agcatgtgga gcggcctgct tcagactcct ggcagaaact
     5461 tgagaagtct cgcgagtata agaacagtaa ccagctccgg gagtaccagc tggaagggat
     5521 gaactggctt ctttttaact ggtataacag aaaaaactgt attttggctg atgagatggg
     5581 cctagggaaa accatccagt ccatcacatt cctttcagaa atatttctga gaggaatcca
     5641 cggccctttt ctcattatcg cccctctctc caccatcact aactgggagc gggagttccg
     5701 gacatggaca gagatgaatg ccattgtgta ccacggcagc cagatcagca ggcagatgat
     5761 ccagcagtat gaaatggtgt acagagacgc ccagggaaac cccctttcag gagtcttcaa
     5821 gttccacgtc gtcatcacaa catttgaaat gatcctagca gactgcccag agttgaagaa
     5881 gattcactgg agctgtgtga taattgatga agcccacaga ctgaagaata ggaactgcaa
     5941 acttctggag ggtctaaagc ttatggccct ggaacataaa gtgcttctca ctggaacacc
     6001 cttgcagaac tctgtggagg agctcttcag tttgttaaat tttctggagc catcacagtt
     6061 tccttcagag accgctttct tggaggaatt tggagatctg aaaacagagg agcaggtaaa
     6121 gaaactgcag tctatcctaa aaccaatgat gcttcggcgg ctgaaagatg atgtggaaaa
     6181 gaaccttgct cccaaacaag agacgatcat tgaggtggaa ctgaccaata tccagaaaaa
     6241 gtactaccgt gccatcctcg agaagaactt ttccttcctg accaaggggg caaatcagca
     6301 caacatgccc aatctcatca acaccatgat ggagctgagg aagtgctgta accatcccta
     6361 cctgatcaat ggagcagagg agaaaattct agaagatttc cgaaaaaccc acagccctga
     6421 tgcccctgac tttcagctgc aggccatgat tcaggcagca ggaaagcttg tgttgattga
     6481 taaactactc cctaagctga ttgcaggtgg ccacaaagta ctcatcttct cccagatggt
     6541 gcgctgcctc gacatcctag aagattacct catccagaga agatacacct atgagcgaat
     6601 tgatgggcga gtacggggaa acctgcgcca ggcagccatc gaccggttct gtaagccaga
     6661 ttcagaccgc tttgtctttc ttctgtgcac cagagcggga ggcctgggga tcaatctcac
     6721 agctgctgat acctgcatca tatttgattc tgactggaac ccacaaaatg acttgcaggc
     6781 tcaggcccga tgtcaccgca taggccagag caaagctgtg aaggtgtatc gcctcatcac
     6841 tcgaaattcc tacgagcgcg agatgtttga caaggccagc ctaaagctgg ggctggacaa
     6901 ggctgttctt caggacatca accgaaaggg cggcaccaat ggggtacagc agctctcaaa
     6961 aatggaggtg gaggacctac tccggaaagg tgcttatgga gccttaatgg atgaagaaga
     7021 tgaaggctcc aagttctgtg aagaagacat agaccagatt ctgcagaggc gaacgcacac
     7081 catcaccatc cagtctgagg ggaaagggtc cacttttgcc aaggctagct ttgtggcttc
     7141 aggaaacaga acagatattt ccttagatga tcctaacttt tggcagaaat gggctaaaat
     7201 agctgaacta gacactgaag caaagaatga aaaggaaagc ttagtgatcg accgacctcg
     7261 cgtgagaaag cagaccaaac actacaactc gtttgaggaa gacgagctca tggagttttc
     7321 agagttagac agcgactcag acgaaaggcc cacgagatcc aggcgcctca atgacaaagc
     7381 caggcgctac ctccgagcgg agtgcttccg ggtagagaag aacctgctca tctttggctg
     7441 gggccggtgg aaggacatcc tgactcatgg ccgattcaag tggcatctga acgagaagga
     7501 catggagatg atttgccgtg ccctcctggt gtactgtgtc aagcattata agggggacga
     7561 gaagatcaag agtttcattt gggaactgat cacacctacc aaagatgggc aagcccagac
     7621 cctccagaac cactcaggct tatctgcccc agtccccaga gggaggaagg ggaagaagac
     7681 gaagaaccag ttgctaatcc cagagctaaa ggatgcagat tggctagcca cctgcaaccc
     7741 cgaggtggtc ttgcatgatg atggctataa gaaacacctc aaacagcact gcaacaaagt
     7801 acttttgcga gtccggatgc tgtactacct aaaagctgaa atactgggag aagcagctga
     7861 gaaagcattt gaaggatctc ctgccaggga gctggatgta cctctgcctg acatcgacta
     7921 catggagatc ccagtggact ggtgggatgc tgaagccgat aagtcacttc tcattggcgt
     7981 gttcaagcat ggttatgaaa ggtacaatgc catgcgagca gacccagcac tttgcttcct
     8041 ggagaaagtt gggatgcccg atgagaagtc cctttctgca gaacagggtg ttacagatgg
     8101 gacctcagac attcctgaaa gaggcaacac agataaagaa gacaatgctg aggacaaagt
     8161 agatggcctc cagaaacaaa cggagagttc cagtgatgga ggtgatggcg tttttagcga
     8221 aaagaaggat gacagccggg cagcccagga tggctccgac ccagacaaat cgccctggcc
     8281 agtttcctcc gccctcacag ctcgtctcag acgtctggtc actgtttacc agcgctgcaa
     8341 ccgcaaggaa ctgtgccggc ctgaaattct gggaccaggt aaccaaggat attgggttca
     8401 ggaagagatg ttcaggagaa cctcagaaat ggacctcatc aacaaggaag cccaaaagag
     8461 gtggactagg agagaacaag cagacttcta tagaacagtg tcttcctttg gtgttgttta
     8521 cgatcaagaa aagaaaacct ttgactggac acagttccgc atcatttccc gtttggacaa
     8581 gaagtcggat gagagcctgg aacagtattt ttatagtttt gtggccatgt gccggaatgt
     8641 ctgtcgtcta cccacatgga aagatggcgg tcccccagat accaccatct acgttgaacc
     8701 catcactgag gaacgtgctg caagaactct gtaccgcatt gaactgttac ggaaagtccg
     8761 agagcaagtg ctcaagtgcc ctcagctgca tgaacgcctc cagctgtgca ggcccagcct
     8821 ctacctccca gtctggtggg agtgtgggaa gcatgatcga gacctgctca tcggcactgc
     8881 caaacatggg ctgaaccgca ctgactgtta catcatgaac gacccccagc tgtccttcct
     8941 ggatgcctat agaaactatg cccagcataa aagatctggc acccaggcac caggaaatct
     9001 ctgttgcctt taccagacca actccaagtt atatgaatct cttacatatt ctcaaatgag
     9061 taggacttca gagtcccttg aaaatgaacc tgaaaatcta gtgagagtag aaagcagaga
     9121 tgatcatctc agcctgcctg atgtgacatg tgaaaacttt atttctaaag ttcaggatgt
     9181 catttccatc aaccatgatg aaagtctgct gcctgagtcc ttagagagca tgatgtatgg
     9241 taagaaggtg ctcagccaag aaccaagctc ttttcaggag agcccaagta ccaatactga
     9301 atctagaaaa gatgttatta ccatctcaat aagcaaagat gggaactgcc agtctggtgg
     9361 ccctgaggca gaaatagctt ctggccctac ttttatgggt agcttagaag caggaggagt
     9421 agctcaagca aacatcaaaa atggaaaaca tttgttgatg tctatttcaa aggaagggga
     9481 gctctgctgc agtgaggcag gacagagacc tgaaaacatt ggccagctgg aagccaagtg
     9541 tttagcttcc ccttccttga atccaggaaa tgaaagtggg tttgtagata tgtgcagtct
     9601 tagtgtctgt gactccaaaa gaaacctgtc atcagatcag caattaattg atttattgga
     9661 aaacaaaagc ttagaaagta aattgatttt gagtcagaac cacagtgatg aggaggaaga
     9721 agaggaggaa aacgaggagg aaaacttagc catggcagta ggcatggggg aaaggccaga
     9781 ggtattgcat ctcacggagc ccactactaa catctcaagg gaaaagaacc aaggcttcca
     9841 agatgaaacc aagaaaggaa gcttagaggt ggcaaaccag actcctgggc tacagagggc
     9901 tttccccgct ccagcagcct gtcagtgcca ctgcaaacac atggagaggt ggatgcatgg
     9961 cctcgagaat gatgaatttg aaatcgagaa acccaaggct tatatcccag atctgttcaa
    10021 aagtaaaacc aatactatcg ccatggaggg tgaacccact gctattccat cacagccgtt
    10081 taaagtgaag catgagcttt taaaagaacc ttggaaagaa agtgcagagg ggcaaaacgt
    10141 tttccccaca tatcctcttg aaggaagtga gctcaaatca gaagacatgg attttgagaa
    10201 taaagatgat tatgatagag acggaaactg ccatagtcaa gattatccag ggaagtactc
    10261 tgaagaggag agcaagagct caacatcggg catcacagga gacattgggg atgagctaca
    10321 ggaggctcga gctcccacta ttgctcagct gctacaggag aaaactctct attccttctc
    10381 tgagtggcca aaggaccgcg tgataattaa ccgcctagat aatatctgcc acgtggtgtt
    10441 aaaggggaag tggccctcta gccagcagta tgagccctca ggcacactgc ccaccccggt
    10501 attaaccagc agtgctggtt ctcgaaccag cctctcagag ccggaagcag cagaacacag
    10561 cttcagcaac ggcgcagcat tggcggccca gatccacaag gagagcttct tagctccagt
    10621 attcacaaag gatgaacaaa agcacaggcg tccctatgag tttgaggtgg agagggatgc
    10681 aaaggctcgg ggcctggagc agttctctgc cacccacggg cacaccccta tcatcctcaa
    10741 tggctggcat ggggagtcag ctatggacct ctcctgctca tcagaggggt ccccaggagc
    10801 cacatcccct ttcccagtga gcgccagcac ccctaagatt ggggctatca gttcacttca
    10861 gggagccctt ggcatggact tgtctgggat tctgcaagct ggcctgatcc atcctgtgac
    10921 tggacagatt gtcaatggaa gcctcagaag agatgatgca gccacgagga ggcggagagg
    10981 gaggcggaaa catgttgaag gagggatgga cctcatcttt ttgaaggagc agacacttca
    11041 ggcgggaatc ttggaagtcc atgaagaccc agggcaggcc accttgagca ccacacaccc
    11101 tgaggggcca gggcctgcca cctcggctcc tgagccagct acggcagcca gcagccaagc
    11161 cgagaaatcc attcccagca agagtctgct tgactggcta aggcagcagg ctgactactc
    11221 cttagaagtt cctggctttg gggcaaattt ttcagacaaa ccaaagcaga ggaggccacg
    11281 ctgtaaagaa cctggaaaat tagatgtcag ctccctgagc ggggaagaga gagttcctgc
    11341 catccccaag gagccaggac tgagggggtt tcttccagaa aacaagttca atcacactct
    11401 ggctgagcct attcttcgag atacgggccc ccgcaggagg gggaggcggc ctcggagcga
    11461 actcctgaag gctccttcca ttgtggcaga ctctccctct ggaatggggc cactgttcat
    11521 gaatggactg attgctggga tggacctggt aggacttcag aacatgagaa atatgccagg
    11581 catccccctc accgggctgg tggggtttcc agctggcttt gccacgatgc caacaggtga
    11641 agaggtcaaa agtaccctga gcatgctgcc catgatgctg ccaggcatgg ctgctgtgcc
    11701 ccagatgttt ggtgttgggg gactcctcag tccacccatg gcaaccacct gcacttccac
    11761 tgctccggcg tctctatcaa gcacaacgaa aagtggtacg gcagtgactg aaaagactgc
    11821 ggaagacaag ccgagtagcc atgatgtgaa aacagacact ttagctgagg acaagcctgg
    11881 tccaggtcca ttttctgatc agtctgaacc tgcaataact actagtagtc ctgtggcttt
    11941 taacccattt ctcatcccag gagtatctcc tggactcatt tacccatcca tgttcctctc
    12001 ccctggtatg ggcatggctc tgccagccat gcagcaggcc agacactcgg aaatagtagg
    12061 tctggagagc cagaagagga agaagaagaa gacaaagggg gacaacccca actcccaccc
    12121 agagcctgct cccagctgtg aaagggagcc cagcggtgat gagaactgtg ccgaacccag
    12181 tgcccctttg cccgcagaga gagaacatgg ggcacaggct ggggaggggg cactcaaaga
    12241 ctccaacaac gacaccaatt agtaatgaac tagtccgtaa cttgaaagta tttcgatttc
    12301 ttggctttat atatcttgtg gaaaggacga tcagttctgc cgggtcacgt ctcgacgcgt
    12361 taagtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
    12421 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
    12481 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
    12541 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
    12601 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
    12661 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
    12721 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
    12781 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
    12841 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
    12901 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcgt
    12961 cgactttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
    13021 aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta
    13081 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
    13141 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
    13201 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
    13261 gcagtacgta tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
    13321 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca
    13381 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
    13441 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc
    13501 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
    13561 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
    13621 aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg
    13681 cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
    13741 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
    13801 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc
    13861 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
    13921 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
    13981 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
    14041 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
    14101 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
    14161 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
    14221 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
    14281 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
    14341 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
    14401 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
    14461 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
    14521 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
    14581 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
    14641 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
    14701 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
    14761 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
    14821 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
    14881 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
    14941 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
    15001 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
    15061 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
    15121 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
    15181 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
    15241 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
    15301 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
    15361 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
    15421 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
    15481 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
    15541 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
    15601 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
    15661 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
    15721 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
    15781 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
    15841 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
    15901 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
    15961 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
    16021 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
    16081 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
    16141 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
    16201 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
    16261 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
    16321 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
    16381 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
    16441 acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc
    16501 tggagctgca agc
//
